CISY - ECOLI - GREMLIN Contacts

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CISY - Citrate synthase

UniProt:	P0ABH7	Sequences: MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFDPGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYDEFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREIAAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILERAMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKMLEEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKDDLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGWIAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10402
Length:	427 (369)
Sequences:	4094 (1947)
Seq/√Len:	101.3

ECOLI - CISY PRPC
BACSU - CISY3 CISY2 CISY
HALSA - Q9HNH4
SULSO - CISY

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
242_A	255_A	7.162	1.00
76_Q	420_D	4.138	1.00
239_V	256_G	3.855	1.00
94_E	100_Q	3.507	1.00
98_Q	102_D	3.453	1.00
292_A	299_F	3.327	1.00
196_G	217_E	3.192	1.00
73_P	76_Q	3.099	1.00
213_N	329_E	3.052	1.00
151_D	154_N	3.019	1.00
81_S	421_F	2.993	1.00
79_T	321_E	2.924	1.00
342_E	345_N	2.924	1.00
193_S	196_G	2.740	1.00
284_H	287_E	2.682	1.00
242_A	259_S	2.634	1.00
97_T	100_Q	2.624	1.00
324_H	341_M	2.606	1.00
247_A	261_W	2.580	1.00
280_S	284_H	2.490	1.00
85_E	101_Y	2.479	1.00
234_A	245_S	2.458	1.00
216_L	373_M	2.423	1.00
277_E	371_K	2.374	1.00
139_T	257_I	2.355	1.00
94_E	419_R	2.345	1.00
295_K	299_F	2.316	1.00
286_P	342_E	2.300	1.00
241_T	407_A	2.282	1.00
197_N	201_M	2.142	1.00
238_T	259_S	2.108	1.00
162_A	393_I	2.065	1.00
195_A	221_D	2.024	1.00
173_A	386_M	2.001	1.00
87_C	390_V	1.961	1.00
287_E	291_R	1.935	1.00
321_E	325_E	1.922	1.00
245_S	259_S	1.911	1.00
286_P	346_I	1.909	1.00
88_Y	94_E	1.844	1.00
48_T	405_K	1.844	1.00
178_K	183_Q	1.836	1.00
215_I	329_E	1.832	1.00
243_G	252_C	1.810	1.00
192_L	196_G	1.809	1.00
82_N	101_Y	1.791	1.00
120_R	123_H	1.789	1.00
282_V	342_E	1.762	1.00
290_R	294_D	1.750	1.00
324_H	328_K	1.738	1.00
218_R	221_D	1.733	1.00
340_A	368_I	1.730	1.00
375_I	383_I	1.721	1.00
116_E	120_R	1.720	1.00
72_F	419_R	1.715	1.00
193_S	217_E	1.715	1.00
138_I	172_M	1.712	1.00
154_N	157_H	1.711	1.00
243_G	396_W	1.710	1.00
223_I	370_L	1.677	1.00
169_M	389_T	1.676	1.00
102_D	106_T	1.668	1.00
270_E	274_K	1.665	1.00
277_E	377_S	1.661	1.00
342_E	346_I	1.657	1.00
137_G	140_G	1.623	1.00
136_C	258_A	1.617	1.00
58_T	229_D	1.605	1.00
291_R	295_K	1.597	1.00
75_D	317_T	1.586	1.00
202_M	375_I	1.572	1.00
351_P	355_E	1.566	1.00
214_P	218_R	1.554	1.00
334_D	371_K	1.539	1.00
136_C	140_G	1.537	1.00
91_L	166_L	1.523	1.00
99_E	103_E	1.512	1.00
222_R	322_T	1.499	1.00
134_V	176_C	1.482	1.00
189_R	200_N	1.472	1.00
107_T	163_F	1.467	1.00
213_N	216_L	1.463	1.00
139_T	169_M	1.458	1.00
189_R	211_E	1.448	1.00
255_A	259_S	1.443	1.00
86_V	225_I	1.440	1.00
223_I	319_M	1.428	1.00
88_Y	100_Q	1.426	1.00
321_E	324_H	1.424	1.00
84_L	108_V	1.424	1.00
111_H	164_R	1.423	1.00
174_A	198_F	1.416	1.00
328_K	334_D	1.405	1.00
188_P	197_N	1.401	1.00
218_R	222_R	1.384	1.00
121_L	141_A	1.372	0.99
234_A	259_S	1.361	0.99
189_R	192_L	1.356	0.99
279_I	292_A	1.354	0.99
76_Q	80_D	1.349	0.99
66_I	73_P	1.346	0.99
106_T	110_R	1.341	0.99
238_T	255_A	1.331	0.99
120_R	124_A	1.329	0.99
112_T	194_Y	1.312	0.99
188_P	201_M	1.310	0.99
282_V	345_N	1.293	0.99
265_H	268_A	1.291	0.99
119_T	175_M	1.290	0.99
323_C	368_I	1.277	0.99
228_A	390_V	1.267	0.99
186_V	201_M	1.261	0.99
112_T	190_N	1.258	0.99
312_Y	317_T	1.254	0.99
180_S	378_S	1.252	0.99
70_R	93_G	1.238	0.99
283_K	287_E	1.231	0.99
48_T	307_R	1.225	0.99
249_P	393_I	1.224	0.99
238_T	242_A	1.220	0.99
72_F	95_K	1.218	0.99
304_F	347_A	1.204	0.99
320_R	344_E	1.200	0.99
251_A	261_W	1.184	0.98
69_H	228_A	1.183	0.98
247_A	251_A	1.179	0.98
289_V	347_A	1.178	0.98
107_T	160_I	1.177	0.98
157_H	161_A	1.175	0.98
161_A	249_P	1.170	0.98
102_D	105_K	1.170	0.98
294_D	298_S	1.161	0.98
238_T	241_T	1.158	0.98
90_L	390_V	1.158	0.98
91_L	393_I	1.153	0.98
89_I	95_K	1.149	0.98
231_E	410_R	1.147	0.98
132_M	269_N	1.140	0.98
301_L	347_A	1.139	0.98
260_L	385_A	1.136	0.98
155_P	401_S	1.136	0.98
81_S	225_I	1.132	0.98
215_I	325_E	1.128	0.98
103_E	107_T	1.128	0.98
123_H	184_P	1.126	0.98
54_E	412_L	1.120	0.98
173_A	382_V	1.114	0.98
105_K	109_T	1.112	0.98
88_Y	92_N	1.111	0.98
92_N	159_E	1.110	0.98
135_M	173_A	1.109	0.98
111_H	163_F	1.109	0.98
281_S	284_H	1.106	0.98
282_V	339_V	1.104	0.98
72_F	89_I	1.097	0.97
320_R	324_H	1.094	0.97
319_M	369_I	1.090	0.97
322_T	325_E	1.089	0.97
253_I	393_I	1.087	0.97
281_S	335_D	1.080	0.97
75_D	318_V	1.079	0.97
203_F	216_L	1.078	0.97
83_Y	390_V	1.069	0.97
387_A	390_V	1.067	0.97
165_L	253_I	1.063	0.97
160_I	164_R	1.059	0.97
264_A	300_R	1.054	0.97
184_P	187_Y	1.043	0.97
253_I	389_T	1.042	0.97
186_V	200_N	1.036	0.96
81_S	85_E	1.034	0.96
397_S	401_S	1.030	0.96
76_Q	79_T	1.030	0.96
239_V	396_W	1.030	0.96
285_I	342_E	1.029	0.96
99_E	102_D	1.029	0.96
371_K	377_S	1.028	0.96
54_E	414_T	1.025	0.96
279_I	285_I	1.019	0.96
182_G	186_V	1.007	0.96
279_I	288_F	0.997	0.96
221_D	225_I	0.997	0.96
82_N	221_D	0.994	0.95
331_G	334_D	0.993	0.95
114_I	175_M	0.992	0.95
247_A	252_C	0.991	0.95
341_M	345_N	0.990	0.95
286_P	290_R	0.989	0.95
275_M	292_A	0.989	0.95
186_V	204_S	0.987	0.95
320_R	341_M	0.985	0.95
113_M	187_Y	0.983	0.95
249_P	396_W	0.973	0.95
159_E	397_S	0.972	0.95
130_H	270_E	0.970	0.95
69_H	86_V	0.968	0.95
114_I	172_M	0.968	0.95
340_A	367_G	0.960	0.95
171_T	188_P	0.958	0.94
276_L	343_L	0.957	0.94
278_E	299_F	0.956	0.94
289_V	346_I	0.955	0.94
196_G	214_P	0.954	0.94
74_I	318_V	0.953	0.94
85_E	421_F	0.946	0.94
94_E	97_T	0.945	0.94
197_N	200_N	0.941	0.94
288_F	292_A	0.938	0.94
136_C	254_A	0.937	0.94
45_P	402_D	0.933	0.94
290_R	351_P	0.925	0.93
240_R	399_M	0.924	0.93
279_I	343_L	0.922	0.93
44_D	414_T	0.921	0.93
292_A	298_S	0.921	0.93
122_F	176_C	0.911	0.93
180_S	184_P	0.908	0.93
277_E	336_L	0.906	0.93
216_L	326_V	0.900	0.92
280_S	335_D	0.899	0.92
271_A	274_K	0.897	0.92
338_E	341_M	0.891	0.92
53_C	232_Q	0.879	0.91
274_K	377_S	0.879	0.91
91_L	390_V	0.877	0.91
140_G	258_A	0.875	0.91
195_A	217_E	0.874	0.91
287_E	290_R	0.873	0.91
340_A	364_F	0.872	0.91
227_H	362_V	0.871	0.91
100_Q	103_E	0.869	0.91
72_F	77_L	0.868	0.91
192_L	197_N	0.866	0.91
90_L	228_A	0.866	0.91
58_T	231_E	0.864	0.91
88_Y	104_F	0.858	0.90
179_Y	183_Q	0.856	0.90
177_Y	181_I	0.856	0.90
269_N	363_D	0.856	0.90
246_G	264_A	0.855	0.90
125_F	144_A	0.844	0.90
64_E	311_N	0.842	0.90
119_T	123_H	0.841	0.90
245_S	406_I	0.837	0.89
224_L	386_M	0.837	0.89
230_H	309_Y	0.835	0.89
348_L	352_Y	0.834	0.89
195_A	220_M	0.834	0.89
118_I	138_I	0.832	0.89
122_F	138_I	0.830	0.89
101_Y	105_K	0.830	0.89
84_L	195_A	0.829	0.89
268_A	361_N	0.821	0.88
218_R	322_T	0.821	0.88
232_Q	410_R	0.820	0.88
166_L	194_Y	0.819	0.88
108_V	163_F	0.819	0.88
323_C	340_A	0.818	0.88
161_A	250_F	0.816	0.88
257_I	385_A	0.816	0.88
272_A	276_L	0.813	0.88
260_L	384_F	0.811	0.88
280_S	283_K	0.811	0.88
282_V	286_P	0.809	0.88
235_S	265_H	0.808	0.88
282_V	338_E	0.805	0.87
354_I	357_K	0.805	0.87
272_A	339_V	0.805	0.87
68_L	418_K	0.803	0.87
192_L	217_E	0.799	0.87
85_E	95_K	0.794	0.87
334_D	338_E	0.794	0.87
68_L	417_E	0.792	0.87
352_Y	355_E	0.791	0.87
266_G	384_F	0.790	0.86
82_N	85_E	0.790	0.86
151_D	157_H	0.789	0.86
237_S	241_T	0.786	0.86
125_F	141_A	0.785	0.86
111_H	160_I	0.783	0.86
121_L	124_A	0.782	0.86
322_T	369_I	0.782	0.86
62_G	65_G	0.780	0.86
218_R	325_E	0.779	0.86
269_N	381_T	0.779	0.86
319_M	366_S	0.776	0.85
107_T	111_H	0.774	0.85
215_I	219_A	0.773	0.85
113_M	190_N	0.773	0.85
171_T	201_M	0.772	0.85
162_A	397_S	0.772	0.85
157_H	160_I	0.771	0.85
183_Q	186_V	0.770	0.85
324_H	337_L	0.769	0.85
312_Y	320_R	0.768	0.85
337_L	341_M	0.768	0.85
205_T	212_V	0.767	0.85
227_H	269_N	0.767	0.85
273_L	380_F	0.766	0.85
265_H	363_D	0.765	0.85
237_S	407_A	0.764	0.85
56_K	414_T	0.764	0.85
148_D	151_D	0.763	0.85
158_R	161_A	0.763	0.85
192_L	212_V	0.762	0.84
146_Y	164_R	0.761	0.84
260_L	266_G	0.759	0.84
58_T	68_L	0.759	0.84
113_M	160_I	0.758	0.84
61_D	64_E	0.757	0.84
216_L	330_L	0.755	0.84
195_A	224_L	0.754	0.84
275_M	288_F	0.751	0.84
87_C	104_F	0.750	0.84
78_A	225_I	0.748	0.83
62_G	311_N	0.748	0.83
252_C	396_W	0.742	0.83
265_H	384_F	0.741	0.83
178_K	186_V	0.740	0.83
87_C	166_L	0.740	0.83
118_I	141_A	0.739	0.83
351_P	354_I	0.733	0.82
294_D	351_P	0.733	0.82
148_D	160_I	0.731	0.82
274_K	277_E	0.729	0.82
181_I	376_P	0.728	0.82
292_A	295_K	0.726	0.81
193_S	221_D	0.725	0.81
304_F	360_P	0.724	0.81
82_N	218_R	0.724	0.81
117_Q	120_R	0.720	0.81
246_G	263_P	0.719	0.81
284_H	291_R	0.719	0.81
278_E	292_A	0.715	0.80
167_S	170_P	0.711	0.80
283_K	286_P	0.708	0.80
83_Y	87_C	0.707	0.80
121_L	125_F	0.706	0.80
118_I	121_L	0.705	0.80
164_R	168_K	0.705	0.80
110_R	190_N	0.705	0.80
290_R	355_E	0.704	0.79
227_H	366_S	0.701	0.79
69_H	89_I	0.700	0.79
47_F	237_S	0.699	0.79
122_F	185_F	0.698	0.79
179_Y	184_P	0.697	0.79
117_Q	145_F	0.696	0.79
135_M	257_I	0.694	0.79
291_R	299_F	0.693	0.78
179_Y	182_G	0.690	0.78
285_I	346_I	0.690	0.78
276_L	340_A	0.687	0.78
336_L	371_K	0.687	0.78
59_F	413_Y	0.687	0.78
82_N	105_K	0.686	0.78
212_V	217_E	0.686	0.78
86_V	228_A	0.684	0.77
175_M	201_M	0.682	0.77
109_T	190_N	0.680	0.77
165_L	249_P	0.679	0.77
158_R	400_H	0.679	0.77
323_C	369_I	0.678	0.77
77_L	86_V	0.675	0.77
333_K	338_E	0.674	0.76
114_I	119_T	0.672	0.76
52_S	237_S	0.667	0.76
77_L	95_K	0.666	0.76
260_L	269_N	0.665	0.76
140_G	254_A	0.662	0.75
123_H	175_M	0.660	0.75
47_F	50_T	0.660	0.75
68_L	73_P	0.657	0.75
115_H	118_I	0.656	0.75
223_I	227_H	0.656	0.75
107_T	110_R	0.655	0.74
59_F	64_E	0.654	0.74
116_E	187_Y	0.653	0.74
353_F	358_L	0.653	0.74
242_A	252_C	0.652	0.74
235_S	266_G	0.651	0.74
148_D	164_R	0.649	0.74
175_M	185_F	0.644	0.73
233_N	362_V	0.641	0.73
301_L	356_K	0.640	0.73
289_V	339_V	0.638	0.72
230_H	384_F	0.637	0.72
402_D	405_K	0.637	0.72
83_Y	221_D	0.635	0.72
192_L	200_N	0.634	0.72
338_E	342_E	0.633	0.72
389_T	393_I	0.632	0.72
362_V	366_S	0.631	0.72
55_S	60_I	0.631	0.72
231_E	411_Q	0.630	0.72
108_V	167_S	0.627	0.71
248_N	251_A	0.627	0.71
416_Y	419_R	0.625	0.71
122_F	179_Y	0.624	0.71
271_A	302_M	0.623	0.71
230_H	233_N	0.622	0.71
222_R	225_I	0.622	0.71
287_E	294_D	0.619	0.70
224_L	387_A	0.619	0.70
286_P	289_V	0.619	0.70
47_F	52_S	0.618	0.70
246_G	400_H	0.617	0.70
300_R	406_I	0.616	0.70
74_I	226_L	0.614	0.70
377_S	380_F	0.613	0.69
236_T	395_H	0.611	0.69
81_S	221_D	0.611	0.69
227_H	363_D	0.609	0.69
139_T	165_L	0.609	0.69
233_N	384_F	0.609	0.69
111_H	168_K	0.609	0.69
335_D	338_E	0.608	0.69
180_S	183_Q	0.607	0.69
174_A	178_K	0.607	0.69
72_F	94_E	0.607	0.69
272_A	362_V	0.605	0.68
320_R	365_Y	0.604	0.68
286_P	354_I	0.604	0.68
162_A	249_P	0.602	0.68
248_N	261_W	0.600	0.68
218_R	225_I	0.599	0.68
230_H	266_G	0.599	0.68
375_I	379_M	0.599	0.68
233_N	309_Y	0.598	0.68
309_Y	392_W	0.598	0.68
236_T	381_T	0.596	0.67
44_D	50_T	0.595	0.67
273_L	377_S	0.595	0.67
195_A	199_L	0.594	0.67
221_D	405_K	0.593	0.67
331_G	422_K	0.592	0.67
143_A	248_N	0.592	0.67
232_Q	240_R	0.592	0.67
158_R	162_A	0.592	0.67
319_M	323_C	0.590	0.67
226_L	318_V	0.588	0.66
285_I	339_V	0.588	0.66
273_L	371_K	0.587	0.66
166_L	390_V	0.586	0.66
385_A	392_W	0.585	0.66
235_S	384_F	0.583	0.66
72_F	76_Q	0.583	0.66
67_L	74_I	0.583	0.66
274_K	278_E	0.581	0.65
344_E	360_P	0.581	0.65
103_E	106_T	0.581	0.65
290_R	293_K	0.580	0.65
246_G	403_G	0.580	0.65
327_L	337_L	0.580	0.65
111_H	256_G	0.579	0.65
174_A	201_M	0.579	0.65
282_V	285_I	0.578	0.65
326_V	369_I	0.577	0.65
163_F	167_S	0.575	0.65
362_V	369_I	0.574	0.64
169_M	172_M	0.573	0.64
144_A	175_M	0.573	0.64
222_R	325_E	0.573	0.64
166_L	393_I	0.572	0.64
323_C	337_L	0.569	0.64
358_L	364_F	0.569	0.64
235_S	363_D	0.569	0.64
182_G	185_F	0.569	0.64
236_T	240_R	0.567	0.63
348_L	355_E	0.566	0.63
70_R	87_C	0.566	0.63
294_D	299_F	0.565	0.63
279_I	289_V	0.565	0.63
275_M	279_I	0.564	0.63
123_H	127_R	0.563	0.63
156_R	160_I	0.562	0.63
310_K	357_K	0.562	0.63
291_R	294_D	0.562	0.63
367_G	384_F	0.561	0.63
263_P	268_A	0.561	0.63
347_A	352_Y	0.558	0.62
223_I	383_I	0.557	0.62
233_N	306_H	0.557	0.62
265_H	305_G	0.557	0.62
49_S	417_E	0.555	0.62
53_C	60_I	0.553	0.62
286_P	324_H	0.552	0.61
121_L	138_I	0.551	0.61
47_F	407_A	0.551	0.61
42_T	294_D	0.550	0.61
55_S	58_T	0.550	0.61
277_E	380_F	0.549	0.61
189_R	197_N	0.549	0.61
288_F	299_F	0.547	0.61
165_L	389_T	0.543	0.60
231_E	358_L	0.539	0.60
273_L	340_A	0.538	0.59
305_G	361_N	0.536	0.59
87_C	91_L	0.535	0.59
59_F	62_G	0.535	0.59
238_T	356_K	0.533	0.59
199_L	217_E	0.533	0.59
273_L	368_I	0.532	0.59
182_G	187_Y	0.532	0.59
132_M	260_L	0.531	0.59
198_F	386_M	0.530	0.58
125_F	137_G	0.529	0.58
319_M	370_L	0.529	0.58
230_H	235_S	0.528	0.58
114_I	171_T	0.528	0.58
47_F	51_A	0.527	0.58
158_R	397_S	0.526	0.58
278_E	282_V	0.526	0.58
140_G	143_A	0.524	0.58
66_I	75_D	0.524	0.58
116_E	184_P	0.523	0.57
290_R	354_I	0.523	0.57
73_P	420_D	0.523	0.57
168_K	171_T	0.523	0.57
128_D	147_H	0.521	0.57
229_D	393_I	0.520	0.57
114_I	118_I	0.520	0.57
396_W	411_Q	0.520	0.57
205_T	211_E	0.520	0.57
118_I	186_V	0.520	0.57
49_S	60_I	0.519	0.57
219_A	369_I	0.519	0.57
252_C	259_S	0.517	0.57
149_S	161_A	0.517	0.57
69_H	226_L	0.517	0.57
232_Q	302_M	0.516	0.56
312_Y	316_A	0.514	0.56
66_I	418_K	0.514	0.56
124_A	128_D	0.513	0.56
183_Q	187_Y	0.513	0.56
87_C	240_R	0.513	0.56
52_S	60_I	0.512	0.56
307_R	310_K	0.510	0.55
77_L	89_I	0.510	0.55
292_A	301_L	0.509	0.55
98_Q	205_T	0.509	0.55
381_T	384_F	0.508	0.55
235_S	260_L	0.508	0.55
240_R	276_L	0.506	0.55
199_L	220_M	0.504	0.55
244_S	318_V	0.504	0.55
293_K	352_Y	0.504	0.55
67_L	71_G	0.502	0.54
283_K	331_G	0.502	0.54
295_K	298_S	0.501	0.54
200_N	211_E	0.500	0.54
188_P	192_L	0.500	0.54
81_S	101_Y	0.500	0.54
61_D	66_I	0.500	0.54

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4xghA	5	0.904	100	0.076	Contact Map
2h12A	4	0.9859	100	0.078	Contact Map
4jadA	5	0.993	100	0.088	Contact Map
4tvmA	2	0.8806	100	0.093	Contact Map
3msuA	2	0.9719	100	0.105	Contact Map
2ibpA	2	0.9251	100	0.122	Contact Map
1iomA	2	0.8735	100	0.128	Contact Map
1aj8A	2	0.8665	100	0.132	Contact Map
1o7xA	2	0.8665	100	0.134	Contact Map
1vgpA	2	0.8712	100	0.134	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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