GREMLIN Database
HYPB - Hydrogenase isoenzymes nickel incorporation protein HypB
UniProt: P0AAN3 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10484
Length: 290 (236)
Sequences: 746 (431)
Seq/√Len: 28.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
247_N272_T4.7131.00
127_R281_L4.4011.00
131_S285_E4.3211.00
123_E127_R4.3001.00
235_M264_I4.1631.00
124_T280_W3.8881.00
257_A264_I3.8161.00
235_M257_A3.4101.00
125_L134_C3.3571.00
212_V238_N3.1041.00
234_L267_I2.9901.00
171_Q175_D2.9721.00
98_R178_P2.7481.00
209_K231_A2.7001.00
83_D89_N2.5010.99
254_I266_I2.4350.99
174_A178_P2.4280.99
144_V148_A2.4190.99
248_F253_C2.3550.99
219_E246_L2.3500.99
236_L269_I2.2650.98
135_A188_L2.1160.97
171_Q174_A2.1000.97
182_L186_G2.0900.97
105_L287_Q2.0890.97
94_R97_A2.0430.97
252_K256_C2.0400.97
254_I268_L1.9390.96
123_E126_M1.9000.95
254_I258_R1.8810.95
105_L187_I1.8370.94
94_R98_R1.8120.94
91_L171_Q1.7840.93
125_L150_I1.7340.92
240_V268_L1.7190.92
252_K255_A1.7130.92
91_L174_A1.7130.92
223_L256_C1.6900.91
98_R174_A1.6880.91
99_F177_A1.6850.91
110_V228_M1.5930.88
206_E209_K1.5820.88
52_Q55_E1.5790.88
98_R102_R1.5290.86
175_D178_P1.5260.86
267_I279_Q1.5180.86
97_A101_A1.5120.86
123_E281_L1.4650.84
216_T221_K1.4560.83
216_T246_L1.4490.83
126_M153_T1.4480.83
176_A188_L1.4350.82
215_V243_L1.4290.82
90_R97_A1.4200.82
60_Y64_E1.4130.81
127_R278_D1.3810.80
181_P186_G1.3810.80
90_R94_R1.3790.80
110_V233_S1.3610.79
60_Y63_G1.3590.79
237_L246_L1.3560.79
250_V254_I1.3560.79
94_R178_P1.3460.78
135_A185_N1.3340.77
160_V171_Q1.3280.77
98_R182_L1.3000.76
121_L136_V1.2800.74
57_D62_H1.2790.74
110_V235_M1.2770.74
243_L248_F1.2650.74
81_E141_Q1.2550.73
238_N271_A1.2530.73
250_V268_L1.2330.72
213_L235_M1.2320.72
175_D179_R1.2310.71
245_Y272_T1.2290.71
261_N265_E1.2280.71
278_D282_N1.2150.70
94_R174_A1.1930.69
237_L250_V1.1910.69
120_L210_V1.1690.67
138_E165_G1.1620.67
91_L178_P1.1550.66
279_Q282_N1.1540.66
63_G71_G1.1280.65
135_A182_L1.1230.64
133_P185_N1.0990.63
91_L175_D1.0980.62
156_P181_P1.0950.62
161_N165_G1.0940.62
99_F106_V1.0900.62
61_G65_A1.0890.62
90_R181_P1.0830.61
58_L61_G1.0810.61
273_S276_G1.0710.60
110_V211_A1.0380.58
269_I281_L1.0350.58
52_Q78_L1.0270.57
148_A152_A1.0240.57
55_E61_G1.0130.56
219_E260_V1.0040.55
213_L253_C1.0020.55
121_L189_F1.0000.55
265_E286_T0.9880.54
209_K253_C0.9770.53
136_V210_V0.9710.53
230_A238_N0.9710.53
59_H63_G0.9680.53
57_D61_G0.9670.53
230_A253_C0.9520.51
106_V186_G0.9510.51
68_H71_G0.9500.51
107_L187_I0.9450.51
219_E262_P0.9380.50
104_Q186_G0.9360.50
84_V144_V0.9330.50
219_E252_K0.9270.50
141_Q161_N0.9250.49
141_Q164_K0.9220.49
208_H233_S0.9220.49
251_E255_A0.9160.49
256_C259_E0.9150.49
134_C187_I0.9130.48
153_T165_G0.9090.48
54_E58_L0.9080.48
58_L64_E0.9060.48
151_R157_A0.9050.48
240_V275_E0.9040.48
216_T260_V0.9040.48
81_E161_N0.9010.48
207_K227_H0.8940.47
66_G78_L0.8910.47
69_A77_M0.8910.47
190_I206_E0.8860.46
133_P181_P0.8850.46
122_T164_K0.8780.46
125_L284_L0.8730.45
81_E244_P0.8730.45
67_T77_M0.8710.45
71_G74_Q0.8700.45
142_Q223_L0.8670.45
164_K227_H0.8670.45
52_Q56_G0.8650.45
141_Q165_G0.8650.45
228_M231_A0.8620.45
61_G64_E0.8600.44
60_Y65_A0.8590.44
161_N283_W0.8580.44
99_F105_L0.8540.44
250_V257_A0.8500.44
54_E57_D0.8480.44
85_L88_N0.8350.43
105_L208_H0.8350.43
141_Q160_V0.8340.42
268_L275_E0.8340.42
233_S265_E0.8310.42
58_L62_H0.8170.41
112_S211_A0.8080.41
130_D232_A0.8070.40
141_Q202_F0.8070.40
126_M134_C0.8050.40
212_V228_M0.8010.40
64_E68_H0.7970.40
70_P232_A0.7950.40
258_R264_I0.7940.40
79_E83_D0.7930.39
219_E266_I0.7890.39
100_A267_I0.7860.39
158_I286_T0.7820.39
97_A102_R0.7810.39
186_G272_T0.7790.38
282_N286_T0.7760.38
87_K123_E0.7730.38
244_P283_W0.7600.37
80_V201_S0.7560.37
112_S214_S0.7490.36
141_Q185_N0.7490.36
120_L277_M0.7480.36
141_Q227_H0.7470.36
233_S282_N0.7440.36
103_K171_Q0.7420.36
56_G177_A0.7390.36
185_N267_I0.7350.35
155_T230_A0.7350.35
129_K233_S0.7330.35
80_V182_L0.7260.35
95_N99_F0.7260.35
211_A234_L0.7240.35
122_T149_R0.7200.34
63_G73_S0.7190.34
240_V250_V0.7130.34
269_I280_W0.7120.34
56_G60_Y0.7090.33
158_I180_L0.7090.33
110_V227_H0.7070.33
120_L236_L0.7060.33
145_N256_C0.7060.33
91_L227_H0.7050.33
115_S176_A0.7040.33
66_G69_A0.7040.33
78_L103_K0.6990.33
58_L66_G0.6970.33
71_G238_N0.6960.33
160_V232_A0.6960.33
202_F244_P0.6950.33
55_E59_H0.6940.32
56_G62_H0.6930.32
208_H212_V0.6920.32
135_A200_A0.6920.32
141_Q201_S0.6890.32
209_K264_I0.6880.32
157_A174_A0.6880.32
61_G73_S0.6860.32
129_K148_A0.6810.32
100_A207_K0.6800.32
106_V177_A0.6790.31
57_D64_E0.6750.31
74_Q77_M0.6750.31
60_Y74_Q0.6730.31
136_V189_F0.6720.31
115_S279_Q0.6720.31
85_L160_V0.6700.31
53_T59_H0.6650.31
86_D89_N0.6650.31
112_S209_K0.6630.30
132_V271_A0.6560.30
122_T284_L0.6550.30
63_G68_H0.6550.30
57_D60_Y0.6550.30
141_Q206_E0.6490.29
111_S248_F0.6480.29
94_R277_M0.6440.29
277_M281_L0.6430.29
204_L227_H0.6390.29
92_A96_R0.6380.29
85_L232_A0.6370.29
133_P186_G0.6350.29
59_H62_H0.6310.28
77_M80_V0.6300.28
107_L257_A0.6300.28
161_N216_T0.6300.28
247_N268_L0.6290.28
124_T210_V0.6250.28
105_L180_L0.6250.28
135_A186_G0.6240.28
101_A123_E0.6230.28
81_E204_L0.6220.28
61_G66_G0.6220.28
90_R101_A0.6220.28
124_T134_C0.6210.28
106_V188_L0.6170.27
133_P246_L0.6160.27
270_S275_E0.6120.27
120_L234_L0.6120.27
263_E284_L0.6110.27
52_Q64_E0.6090.27
96_R99_F0.6090.27
56_G59_H0.6050.27
102_R184_D0.6050.27
258_R278_D0.6050.27
141_Q150_I0.6040.27
93_E122_T0.6030.27
80_V284_L0.6010.27
201_S227_H0.5990.26
63_G74_Q0.5960.26
135_A176_A0.5950.26
125_L155_T0.5940.26
77_M201_S0.5930.26
61_G69_A0.5920.26
60_Y66_G0.5920.26
110_V231_A0.5890.26
70_P77_M0.5840.26
184_D233_S0.5820.25
213_L264_I0.5810.25
147_A176_A0.5810.25
129_K282_N0.5790.25
88_N92_A0.5780.25
211_A228_M0.5770.25
222_P276_G0.5760.25
213_L237_L0.5740.25
99_F231_A0.5710.25
245_Y283_W0.5700.25
109_L119_T0.5680.25
60_Y71_G0.5660.24
104_Q182_L0.5660.24
157_A213_L0.5660.24
55_E58_L0.5640.24
199_P209_K0.5640.24
124_T284_L0.5630.24
53_T58_L0.5630.24
237_L243_L0.5620.24
169_D234_L0.5620.24
84_V201_S0.5590.24
230_A259_E0.5590.24
67_T74_Q0.5590.24
119_T124_T0.5580.24
150_I203_D0.5580.24
110_V209_K0.5570.24
112_S228_M0.5550.24
110_V208_H0.5550.24
85_L92_A0.5520.24
119_T271_A0.5520.24
234_L286_T0.5500.24
236_L258_R0.5490.24
90_R93_E0.5480.23
159_Q203_D0.5470.23
211_A264_I0.5450.23
236_L244_P0.5450.23
189_F230_A0.5430.23
90_R174_A0.5430.23
189_F267_I0.5430.23
75_R78_L0.5420.23
105_L175_D0.5410.23
158_I179_R0.5410.23
269_I277_M0.5400.23
110_V264_I0.5400.23
111_S228_M0.5380.23
54_E60_Y0.5380.23
142_Q188_L0.5370.23
110_V212_V0.5370.23
188_L204_L0.5370.23
272_T282_N0.5370.23
102_R133_P0.5340.23
70_P74_Q0.5340.23
76_R79_E0.5340.23
74_Q86_D0.5310.23
53_T130_D0.5290.22
105_L264_I0.5290.22
223_L260_V0.5280.22
122_T201_S0.5270.22
129_K235_M0.5230.22
169_D227_H0.5220.22
64_E77_M0.5220.22
86_D283_W0.5220.22
110_V230_A0.5210.22
81_E202_F0.5210.22
139_G149_R0.5210.22
181_P185_N0.5180.22
55_E183_D0.5180.22
161_N202_F0.5170.22
138_E220_D0.5140.22
282_N285_E0.5140.22
75_R79_E0.5130.22
52_Q62_H0.5120.22
54_E63_G0.5100.21
187_I208_H0.5100.21
130_D263_E0.5090.21
153_T157_A0.5080.21
101_A263_E0.5050.21
104_Q251_E0.5050.21
86_D212_V0.5050.21
138_E153_T0.5040.21
126_M237_L0.5030.21
98_R101_A0.5000.21
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2wsmA 2 0.7069 100 0.716 Contact Map
2hf9A 2 0.7207 100 0.725 Contact Map
4hi0E 2 0.6379 100 0.756 Contact Map
4lpsA 1 0.7 100 0.758 Contact Map
2qm8A 2 0.8 99.9 0.797 Contact Map
2wwwA 2 0.7552 99.9 0.813 Contact Map
3p32A 2 0.7345 99.9 0.814 Contact Map
1yrbA 2 0.6724 99.8 0.843 Contact Map
3avxA 1 0.7276 99.8 0.845 Contact Map
4xc8A 2 0.8862 99.8 0.848 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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