GREMLIN Database
HYBE - Hydrogenase-2 operon protein HybE
UniProt: P0AAN1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11803
Length: 162 (145)
Sequences: 228 (185)
Seq/√Len: 15.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_Y124_Q4.8271.00
20_F105_Y4.0981.00
42_V45_F3.9781.00
62_L114_L3.2170.99
89_Y92_M3.1890.99
88_P135_L3.1410.99
149_R152_L2.9140.98
52_W69_G2.5900.95
111_M126_L2.5110.94
45_F54_G2.4630.94
39_P120_I2.2960.91
90_G135_L2.1810.88
17_Q42_V2.1530.88
149_R153_L2.1170.87
86_Q91_T2.0850.86
94_F155_G2.0790.86
82_K93_T2.0370.85
109_S112_S2.0270.84
69_G74_W1.9420.82
20_F40_V1.7810.76
129_D133_M1.7610.75
87_L134_I1.7180.73
66_I88_P1.7150.73
57_I128_D1.6820.71
48_F135_L1.6600.70
85_L106_L1.5750.66
94_F108_C1.5670.66
98_E104_Q1.5610.65
111_M117_S1.5590.65
12_P52_W1.5300.64
129_D132_R1.5290.64
87_L97_G1.5090.62
55_C62_L1.4680.60
50_G129_D1.4660.60
56_V86_Q1.4440.59
19_A23_I1.3910.56
148_G152_L1.3250.52
84_G126_L1.3140.52
121_E136_S1.3100.51
32_S80_S1.2770.50
27_S56_V1.2490.48
67_F100_D1.2460.48
89_Y130_C1.2120.46
49_E122_E1.2070.46
40_V103_S1.2040.45
82_K107_S1.1660.43
27_S99_L1.1600.43
74_W77_R1.1580.43
88_P154_F1.1480.42
12_P88_P1.1400.42
20_F155_G1.1350.42
23_I115_S1.1350.42
65_V97_G1.1230.41
57_I124_Q1.1130.40
46_T88_P1.0970.40
65_V116_H1.0930.39
11_S15_Q1.0910.39
43_S129_D1.0880.39
56_V105_Y1.0800.39
46_T135_L1.0770.38
58_T113_P1.0690.38
147_A151_A1.0670.38
119_S122_E1.0420.37
35_H38_M1.0350.36
64_A134_I1.0290.36
44_D123_G1.0150.35
62_L111_M1.0000.34
118_M122_E0.9990.34
55_C130_C0.9990.34
52_W138_P0.9940.34
58_T126_L0.9930.34
78_K81_E0.9920.34
44_D128_D0.9750.33
97_G112_S0.9570.32
62_L117_S0.9480.32
119_S125_R0.9300.31
33_F51_Q0.9250.31
93_T111_M0.9240.31
147_A154_F0.9200.30
47_L52_W0.9180.30
57_I86_Q0.9100.30
150_R153_L0.9040.30
18_A22_E0.8850.29
52_W66_I0.8700.28
27_S30_D0.8680.28
83_I97_G0.8630.28
56_V68_P0.8520.27
74_W105_Y0.8500.27
16_V128_D0.8470.27
23_I151_A0.8450.27
107_S112_S0.8430.27
86_Q126_L0.8400.27
36_P139_V0.8380.27
30_D134_I0.8360.27
15_Q125_R0.8350.26
114_L117_S0.8340.26
117_S127_T0.8340.26
143_D149_R0.8330.26
67_F105_Y0.8240.26
126_L130_C0.8050.25
77_R85_L0.8030.25
20_F102_V0.8030.25
118_M145_P0.8000.25
144_V153_L0.8000.25
38_M114_L0.7990.25
94_F110_L0.7950.25
13_K20_F0.7950.25
26_R36_P0.7890.24
30_D131_A0.7790.24
17_Q23_I0.7730.24
43_S57_I0.7670.24
53_T131_A0.7610.23
22_E26_R0.7560.23
13_K100_D0.7550.23
32_S110_L0.7480.23
22_E78_K0.7480.23
95_T123_G0.7450.23
40_V140_T0.7420.23
16_V29_H0.7410.23
76_L119_S0.7390.22
89_Y93_T0.7320.22
22_E102_V0.7250.22
142_P151_A0.7240.22
142_P146_H0.7220.22
55_C131_A0.7200.22
18_A82_K0.7190.22
19_A102_V0.7120.21
27_S119_S0.7120.21
24_A85_L0.7060.21
71_D138_P0.7060.21
84_G92_M0.7000.21
12_P47_L0.6990.21
80_S123_G0.6950.21
46_T90_G0.6940.21
45_F127_T0.6900.21
38_M123_G0.6890.21
36_P101_G0.6840.20
52_W148_G0.6790.20
24_A34_L0.6780.20
96_V152_L0.6690.20
96_V133_M0.6690.20
49_E72_Q0.6600.19
124_Q128_D0.6590.19
14_A70_P0.6490.19
125_R152_L0.6480.19
48_F75_P0.6470.19
48_F66_I0.6430.19
57_I132_R0.6420.19
52_W136_S0.6420.19
33_F68_P0.6420.19
140_T143_D0.6380.19
58_T121_E0.6360.19
16_V67_F0.6350.19
29_H50_G0.6330.19
35_H120_I0.6310.18
50_G120_I0.6250.18
46_T136_S0.6230.18
54_G59_P0.6220.18
31_L47_L0.6190.18
17_Q20_F0.6190.18
55_C80_S0.6180.18
16_V64_A0.6150.18
114_L132_R0.6140.18
61_M118_M0.6130.18
26_R96_V0.6090.18
39_P87_L0.6070.18
56_V99_L0.6070.18
85_L138_P0.6000.17
12_P74_W0.5980.17
99_L105_Y0.5960.17
86_Q98_E0.5940.17
28_M109_S0.5910.17
105_Y110_L0.5880.17
133_M136_S0.5870.17
35_H117_S0.5740.17
93_T96_V0.5710.17
83_I106_L0.5640.16
83_I96_V0.5620.16
32_S123_G0.5600.16
39_P51_Q0.5600.16
23_I94_F0.5580.16
70_P97_G0.5580.16
12_P58_T0.5570.16
35_H46_T0.5540.16
14_A66_I0.5540.16
40_V90_G0.5530.16
14_A86_Q0.5510.16
34_L107_S0.5500.16
20_F99_L0.5490.16
70_P139_V0.5460.16
65_V107_S0.5430.16
62_L95_T0.5420.16
15_Q135_L0.5420.16
17_Q41_Y0.5420.16
64_A135_L0.5410.16
126_L135_L0.5400.16
132_R142_P0.5360.15
70_P119_S0.5350.15
87_L90_G0.5330.15
16_V56_V0.5330.15
17_Q105_Y0.5300.15
68_P77_R0.5290.15
56_V73_L0.5250.15
61_M72_Q0.5250.15
53_T87_L0.5250.15
25_R140_T0.5250.15
74_W135_L0.5240.15
13_K30_D0.5230.15
57_I134_I0.5220.15
14_A22_E0.5190.15
16_V41_Y0.5170.15
143_D147_A0.5150.15
31_L34_L0.5110.15
43_S111_M0.5110.15
51_Q72_Q0.5100.15
22_E29_H0.5070.15
144_V149_R0.5020.14
92_M118_M0.5000.14
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2kc5A 1 0.9938 100 0.12 Contact Map
5aj3o 1 0.216 8.9 0.958 Contact Map
1q90R 1 0.1358 7 0.96 Contact Map
2pq4B 1 0.1111 4.9 0.963 Contact Map
4jdcA 5 0.9506 4.9 0.963 Contact Map
2puzA 2 0.358 4.2 0.964 Contact Map
3ecmA 1 0.4938 3.8 0.965 Contact Map
2a2lA 5 0.3272 2.9 0.967 Contact Map
2imhA 2 0.3333 2.3 0.969 Contact Map
4i15A 2 0.4815 2.3 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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