GREMLIN Database
HYBA - Hydrogenase-2 operon protein HybA
UniProt: P0AAJ8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11799
Length: 328 (263)
Sequences: 569 (346)
Seq/√Len: 21.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
132_D135_T5.4801.00
194_V202_V4.6471.00
215_K250_L3.9511.00
162_Y171_L3.6091.00
215_K253_E3.0781.00
265_T269_Y3.0060.99
213_E216_K2.9840.99
65_R83_T2.9630.99
121_V129_L2.9490.99
60_I192_G2.8610.99
211_M250_L2.8010.99
203_I213_E2.7070.99
54_V76_N2.7040.99
60_I183_E2.5740.98
45_T266_G2.5020.98
85_N109_K2.4600.98
40_M261_V2.4010.97
39_G210_L2.3390.97
118_P131_K2.3340.97
132_D139_H2.3010.96
42_Y261_V2.3000.96
116_V260_Q2.2230.96
207_R211_M2.2210.96
178_N186_D2.1810.95
125_P146_T2.1800.95
211_M253_E2.1790.95
78_K161_K2.1730.95
183_E192_G2.1390.94
252_G255_E2.1150.94
61_N175_E2.0990.94
30_N33_P2.0870.94
184_R195_E1.9460.91
149_R152_M1.9140.90
41_L262_L1.8500.89
212_A216_K1.8330.88
48_V199_A1.8250.88
319_H322_G1.8000.87
29_E32_P1.7840.87
117_D122_S1.7200.85
298_M302_A1.7190.85
152_M171_L1.7020.84
36_G208_E1.6920.84
38_L137_I1.6910.84
31_R34_I1.6690.83
317_N322_G1.6530.82
78_K88_Q1.6160.81
212_A215_K1.5960.80
40_M202_V1.5740.79
296_G300_P1.5730.79
211_M215_K1.5690.79
295_K299_L1.5560.78
41_L210_L1.5550.78
58_Q83_T1.5310.77
43_D258_G1.5240.77
206_T209_E1.5050.76
152_M162_Y1.4950.75
149_R162_Y1.4790.74
290_Q293_L1.4480.73
272_L279_D1.4340.72
32_P35_P1.4060.71
42_Y176_L1.4060.71
142_K153_V1.3980.70
65_R68_Q1.3970.70
108_I287_E1.3870.70
61_N176_L1.3790.69
157_Y191_P1.3690.68
87_I107_Y1.3620.68
160_P257_G1.3360.67
126_V149_R1.3270.66
42_Y111_Q1.3260.66
46_L203_I1.3120.65
315_T319_H1.3110.65
37_S116_V1.2950.64
31_R35_P1.2920.64
33_P36_G1.2910.64
302_A311_V1.2890.64
309_V314_N1.2880.64
316_K319_H1.2880.64
318_D322_G1.2870.64
163_D171_L1.2850.63
211_M252_G1.2450.61
48_V52_A1.2390.61
137_I185_L1.2330.60
210_L262_L1.2230.60
99_D102_E1.2220.59
210_L264_L1.2220.59
38_L206_T1.2110.59
308_T314_N1.2100.59
274_L277_L1.2040.58
308_T313_R1.2010.58
157_Y259_T1.1890.57
159_V316_K1.1730.56
118_P135_T1.1670.56
313_R317_N1.1630.56
250_L262_L1.1620.56
130_K141_D1.1550.55
81_P163_D1.1450.54
205_G210_L1.1420.54
184_R189_G1.1400.54
54_V75_N1.1390.54
163_D166_N1.1380.54
196_V313_R1.1380.54
29_E34_I1.1360.54
85_N111_Q1.1330.54
208_E212_A1.1310.53
98_K102_E1.1290.53
317_N320_H1.1270.53
251_Y258_G1.1250.53
109_K261_V1.1240.53
80_S159_V1.1220.53
216_K219_A1.1080.52
44_S106_A1.1000.51
46_L200_G1.0850.50
302_A310_L1.0790.50
290_Q294_Y1.0750.50
249_H253_E1.0740.50
249_H255_E1.0740.50
213_E296_G1.0670.49
294_Y300_P1.0610.49
305_A312_R1.0570.49
42_Y263_V1.0550.48
154_A261_V1.0450.48
44_S265_T1.0400.47
312_R316_K1.0390.47
51_Q76_N1.0350.47
140_Y154_A1.0310.47
286_S291_H1.0250.46
162_Y265_T1.0210.46
221_K225_E1.0180.46
176_L261_V1.0170.46
60_I184_R1.0170.46
170_A224_S1.0140.46
258_G263_V1.0130.46
118_P257_G1.0080.45
29_E33_P1.0030.45
143_D293_L0.9940.44
29_E36_G0.9910.44
111_Q261_V0.9840.44
51_Q225_E0.9790.43
215_K219_A0.9700.43
30_N208_E0.9680.43
123_V150_Y0.9640.42
38_L204_F0.9580.42
90_W275_P0.9530.42
191_P194_V0.9510.42
28_A31_R0.9480.41
79_L186_D0.9420.41
290_Q300_P0.9370.41
109_K148_C0.9360.41
162_Y297_M0.9350.40
284_A303_V0.9310.40
61_N200_G0.9300.40
277_L280_L0.9280.40
272_L275_P0.9250.40
69_G117_D0.9240.40
128_A144_V0.9220.40
84_N172_H0.9200.40
30_N34_I0.9190.39
42_Y146_T0.9160.39
40_M194_V0.9140.39
63_P84_N0.9120.39
66_N80_S0.9120.39
37_S91_T0.9100.39
42_Y109_K0.9100.39
74_S170_A0.9070.39
42_Y125_P0.9060.39
211_M218_L0.8820.37
287_E294_Y0.8740.37
290_Q304_L0.8680.36
37_S260_Q0.8660.36
90_W273_D0.8630.36
122_S287_E0.8570.36
80_S264_L0.8500.35
129_L140_Y0.8490.35
216_K220_L0.8490.35
140_Y169_G0.8460.35
256_G274_L0.8450.35
287_E291_H0.8430.35
304_L307_L0.8400.35
195_E221_K0.8310.34
63_P66_N0.8270.34
61_N84_N0.8260.34
279_D321_D0.8240.34
218_L264_L0.8240.34
298_M301_L0.8230.33
132_D137_I0.8220.33
312_R319_H0.8170.33
35_P100_Q0.8100.33
54_V111_Q0.8090.33
310_L315_T0.8070.33
52_A59_D0.8020.32
72_T88_Q0.7920.32
111_Q152_M0.7830.31
121_V199_A0.7800.31
32_P142_K0.7780.31
141_D144_V0.7780.31
218_L223_G0.7770.31
135_T139_H0.7770.31
170_A209_E0.7750.31
122_S286_S0.7720.31
38_L176_L0.7690.30
48_V161_K0.7690.30
36_G206_T0.7680.30
131_K200_G0.7650.30
81_P162_Y0.7630.30
104_G202_V0.7590.30
119_N154_A0.7580.30
91_T103_N0.7580.30
199_A291_H0.7580.30
54_V71_Q0.7570.30
62_F65_R0.7560.30
315_T318_D0.7550.30
101_E264_L0.7540.30
296_G302_A0.7540.30
51_Q74_S0.7520.29
70_E73_W0.7500.29
163_D170_A0.7490.29
215_K254_K0.7460.29
214_A217_R0.7460.29
44_S48_V0.7440.29
59_D159_V0.7370.29
118_P259_T0.7370.29
42_Y274_L0.7280.28
295_K311_V0.7280.28
34_I37_S0.7270.28
75_N89_V0.7270.28
308_T312_R0.7250.28
305_A318_D0.7140.27
55_T166_N0.7130.27
45_T217_R0.7130.27
185_L192_G0.7100.27
141_D216_K0.7010.27
52_A196_V0.6990.27
111_Q263_V0.6970.26
39_G204_F0.6970.26
297_M300_P0.6960.26
163_D169_G0.6950.26
118_P280_L0.6920.26
42_Y106_A0.6910.26
126_V154_A0.6880.26
54_V127_S0.6860.26
190_L302_A0.6810.26
309_V313_R0.6790.26
87_I132_D0.6790.26
299_L302_A0.6750.25
123_V146_T0.6740.25
64_E79_L0.6740.25
44_S263_V0.6720.25
190_L194_V0.6720.25
54_V86_I0.6710.25
168_F201_A0.6710.25
32_P36_G0.6670.25
221_K264_L0.6620.25
139_H178_N0.6550.24
81_P322_G0.6540.24
311_V320_H0.6530.24
146_T202_V0.6510.24
185_L302_A0.6500.24
141_D178_N0.6470.24
52_A161_K0.6470.24
162_Y212_A0.6470.24
152_M261_V0.6450.24
85_N88_Q0.6450.24
60_I74_S0.6440.24
52_A60_I0.6390.24
297_M309_V0.6390.24
58_Q295_K0.6370.23
175_E224_S0.6360.23
170_A284_A0.6330.23
92_S162_Y0.6300.23
277_L313_R0.6300.23
82_Y167_P0.6290.23
277_L283_G0.6270.23
159_V248_P0.6260.23
79_L129_L0.6250.23
198_P202_V0.6250.23
79_L161_K0.6220.23
28_A60_I0.6220.23
226_Y269_Y0.6210.23
208_E320_H0.6200.23
287_E292_T0.6190.23
290_Q296_G0.6180.23
186_D250_L0.6150.22
149_R153_V0.6150.22
158_N202_V0.6120.22
109_K263_V0.6110.22
249_H266_G0.6100.22
79_L175_E0.6090.22
69_G72_T0.6090.22
33_P134_K0.6070.22
69_G319_H0.6070.22
129_L202_V0.6060.22
222_P225_E0.6060.22
269_Y272_L0.6010.22
219_A250_L0.5980.22
122_S201_A0.5930.21
135_T260_Q0.5930.21
169_G225_E0.5920.21
31_R142_K0.5890.21
63_P82_Y0.5890.21
102_E322_G0.5870.21
98_K298_M0.5850.21
207_R262_L0.5850.21
137_I178_N0.5840.21
149_R253_E0.5840.21
305_A309_V0.5820.21
149_R294_Y0.5800.21
46_L98_K0.5790.21
134_K200_G0.5770.21
87_I137_I0.5720.20
41_L264_L0.5690.20
280_L283_G0.5690.20
80_S170_A0.5680.20
64_E172_H0.5680.20
31_R208_E0.5660.20
198_P259_T0.5660.20
30_N35_P0.5640.20
295_K298_M0.5640.20
39_G44_S0.5580.20
148_C157_Y0.5570.20
67_P80_S0.5570.20
253_E262_L0.5560.20
35_P141_D0.5500.20
265_T268_P0.5480.19
253_E286_S0.5480.19
48_V76_N0.5460.19
118_P160_P0.5460.19
213_E219_A0.5450.19
80_S320_H0.5430.19
30_N36_G0.5420.19
303_V311_V0.5410.19
215_K262_L0.5370.19
212_A270_E0.5360.19
64_E84_N0.5360.19
86_I194_V0.5310.19
252_G258_G0.5310.19
52_A116_V0.5300.19
58_Q108_I0.5290.19
109_K198_P0.5260.19
156_P193_C0.5250.18
125_P300_P0.5240.18
299_L307_L0.5230.18
312_R318_D0.5220.18
81_P153_V0.5200.18
304_L308_T0.5180.18
55_T301_L0.5170.18
40_M111_Q0.5150.18
163_D256_G0.5120.18
293_L306_G0.5110.18
194_V201_A0.5100.18
40_M152_M0.5060.18
59_D317_N0.5060.18
281_S287_E0.5060.18
41_L135_T0.5050.18
313_R316_K0.5010.18
47_C197_C0.5000.18
124_C155_C0.5000.18
47_C50_C0.5000.18
47_C53_C0.5000.18
47_C57_C0.5000.18
47_C120_C0.5000.18
47_C174_C0.5000.18
47_C173_K0.5000.18
47_C155_C0.5000.18
47_C112_C0.5000.18
47_C151_C0.5000.18
47_C115_C0.5000.18
47_C124_C0.5000.18
47_C145_C0.5000.18
145_C155_C0.5000.18
124_C174_C0.5000.18
124_C173_K0.5000.18
124_C145_C0.5000.18
53_C197_C0.5000.18
120_C197_C0.5000.18
120_C174_C0.5000.18
120_C173_K0.5000.18
120_C155_C0.5000.18
120_C151_C0.5000.18
120_C145_C0.5000.18
124_C197_C0.5000.18
53_C174_C0.5000.18
145_C151_C0.5000.18
53_C112_C0.5000.18
53_C115_C0.5000.18
57_C197_C0.5000.18
53_C120_C0.5000.18
53_C124_C0.5000.18
53_C145_C0.5000.18
53_C151_C0.5000.18
53_C155_C0.5000.18
53_C173_K0.5000.18
57_C173_K0.5000.18
115_C197_C0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1kqfB 3 0.8445 100 0.529 Contact Map
1h0hB 1 0.6341 100 0.629 Contact Map
2ivfB 1 0.7927 100 0.655 Contact Map
1ti6B 2 0.7591 100 0.68 Contact Map
2vpzB 2 0.5884 100 0.704 Contact Map
1q16B 2 0.8628 100 0.715 Contact Map
4z3xE 2 0.5183 99.9 0.783 Contact Map
3mm5B 2 0.7256 99.8 0.831 Contact Map
2fgoA 1 0.2317 99.4 0.88 Contact Map
1rgvA 1 0.2409 99.3 0.884 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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