GREMLIN Database
PFLA - Pyruvate formate-lyase 1-activating enzyme
UniProt: P0A9N4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10028
Length: 246 (217)
Sequences: 4038 (3053)
Seq/√Len: 207.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
61_V98_E4.1811.00
74_V95_C3.1821.00
136_D146_S2.9281.00
76_A88_V2.8401.00
168_Y197_I2.8001.00
93_R123_V2.7301.00
94_A97_K2.5451.00
92_F102_T2.5321.00
136_D148_H2.4531.00
53_V94_A2.4101.00
96_K125_D2.3281.00
89_R119_E2.2931.00
54_E94_A2.2101.00
129_L154_A2.2071.00
166_I188_T2.2031.00
76_A102_T2.1471.00
25_T56_L2.0461.00
94_A98_E2.0451.00
8_H42_T2.0081.00
140_Q146_S1.9701.00
108_G129_L1.9541.00
127_V157_L1.9531.00
138_I180_S1.8831.00
31_L107_N1.8811.00
155_K190_D1.8661.00
118_D156_Y1.8631.00
163_K195_E1.8581.00
138_I141_N1.8451.00
148_H152_E1.8181.00
155_K159_N1.7751.00
76_A91_W1.7561.00
35_L218_K1.7211.00
158_A192_G1.6841.00
152_E155_K1.6731.00
178_D182_H1.6631.00
142_L174_W1.6401.00
76_A92_F1.6351.00
156_Y160_K1.6311.00
89_R93_R1.6121.00
226_K229_T1.5931.00
32_M109_F1.5891.00
183_R186_E1.5551.00
127_V162_V1.5531.00
54_E58_K1.5501.00
112_R152_E1.5441.00
216_E220_D1.5411.00
112_R156_Y1.5361.00
179_D183_R1.5261.00
57_M95_C1.5071.00
23_F59_E1.4981.00
7_I27_F1.4811.00
81_A104_L1.4601.00
118_D160_K1.4421.00
57_M94_A1.4341.00
139_H147_N1.4291.00
101_H126_L1.4221.00
177_D180_S1.4101.00
11_E14_G1.4081.00
92_F124_T1.4061.00
131_L154_A1.3851.00
133_Q138_I1.3801.00
168_Y184_L1.3631.00
135_N183_R1.3621.00
178_D229_T1.3541.00
154_A166_I1.3501.00
32_M107_N1.3261.00
88_V104_L1.3141.00
26_F79_G1.2861.00
35_L141_N1.2791.00
134_M187_F1.2581.00
182_H186_E1.2421.00
61_V64_R1.2331.00
111_R116_V1.2311.00
27_F76_A1.2281.00
51_V56_L1.2201.00
60_V95_C1.2161.00
164_V193_N1.2101.00
104_L127_V1.2001.00
134_M183_R1.1831.00
134_M180_S1.1761.00
173_G222_V1.1581.00
165_W196_K1.1541.00
22_R75_T1.1391.00
59_E62_T1.1341.00
52_T55_D1.1311.00
106_T129_L1.1311.00
82_I110_V1.1241.00
112_R118_D1.1181.00
211_V220_D1.1081.00
31_L109_F1.1021.00
157_L164_V1.1011.00
22_R73_G1.0951.00
133_Q142_L1.0901.00
95_C100_I1.0891.00
7_I51_V1.0881.00
133_Q180_S1.0881.00
170_V199_L1.0801.00
225_P229_T1.0751.00
93_R119_E1.0731.00
53_V91_W1.0721.00
64_R68_N1.0611.00
192_G195_E1.0601.00
137_E141_N1.0591.00
81_A88_V1.0561.00
31_L83_L1.0551.00
151_L187_F1.0531.00
38_H105_D1.0501.00
96_K123_V1.0441.00
128_M167_R1.0431.00
156_Y159_N1.0401.00
139_H150_T1.0401.00
119_E122_E1.0341.00
188_T197_I1.0281.00
155_K187_F1.0121.00
117_I153_F1.0121.00
55_D58_K1.0091.00
154_A191_M1.0051.00
19_P126_L0.9981.00
52_T87_F0.9961.00
124_T127_V0.9921.00
223_K227_K0.9881.00
93_R97_K0.9771.00
109_F149_R0.9771.00
57_M61_V0.9721.00
49_K55_D0.9711.00
110_V116_V0.9500.99
185_G199_L0.9460.99
89_R123_V0.9450.99
85_A116_V0.9390.99
24_I77_S0.9370.99
138_I177_D0.9340.99
105_D128_M0.9340.99
33_R43_W0.9300.99
45_T83_L0.9290.99
174_W211_V0.9280.99
131_L184_L0.9210.99
204_E207_K0.9110.99
88_V92_F0.9050.99
115_P118_D0.9000.99
18_G22_R0.8850.99
18_G101_H0.8850.99
43_W215_E0.8840.99
121_L160_K0.8810.99
154_A187_F0.8800.99
168_Y180_S0.8770.99
178_D221_G0.8710.99
61_V100_I0.8700.99
170_V181_A0.8680.99
56_L91_W0.8650.99
60_V74_V0.8620.99
26_F39_N0.8610.99
148_H151_L0.8510.99
186_E189_R0.8500.99
158_A164_V0.8490.99
57_M60_V0.8410.99
49_K59_E0.8390.99
158_A191_M0.8370.99
18_G73_G0.8360.99
109_F145_V0.8300.99
59_E63_Y0.8200.98
223_K229_T0.8180.98
121_L162_V0.8180.98
82_I107_N0.8170.98
56_L87_F0.8120.98
108_G153_F0.8100.98
96_K102_T0.8090.98
60_V91_W0.8040.98
82_I104_L0.8040.98
122_E160_K0.8020.98
95_C102_T0.8000.98
118_D122_E0.8000.98
35_L142_L0.7990.98
53_V90_D0.7990.98
101_H125_D0.7970.98
182_H228_E0.7900.98
53_V57_M0.7870.98
147_N151_L0.7860.98
187_F191_M0.7850.98
51_V55_D0.7760.98
102_T124_T0.7750.98
14_G24_I0.7680.98
175_S180_S0.7610.97
135_N180_S0.7600.97
176_D229_T0.7580.97
27_F84_Q0.7550.97
180_S184_L0.7540.97
35_L174_W0.7520.97
57_M98_E0.7510.97
103_C126_L0.7500.97
105_D165_W0.7490.97
172_P176_D0.7470.97
35_L143_V0.7430.97
207_K221_G0.7410.97
85_A89_R0.7390.97
89_R120_L0.7350.97
36_Y171_V0.7290.97
218_K221_G0.7280.97
27_F81_A0.7240.97
32_M150_T0.7240.97
207_K220_D0.7240.97
7_I25_T0.7230.97
179_D182_H0.7160.96
152_E156_Y0.7160.96
215_E218_K0.7110.96
36_Y41_D0.7090.96
58_K61_V0.7080.96
147_N150_T0.7050.96
45_T48_G0.7040.96
12_S63_Y0.7040.96
11_E24_I0.6970.96
124_T162_V0.6940.96
137_E140_Q0.6930.96
133_Q143_V0.6930.96
188_T191_M0.6920.96
63_Y67_M0.6910.96
86_E116_V0.6890.95
36_Y142_L0.6890.95
186_E229_T0.6880.95
136_D147_N0.6870.95
155_K191_M0.6850.95
185_G189_R0.6840.95
168_Y181_A0.6840.95
22_R103_C0.6810.95
103_C128_M0.6720.95
118_D159_N0.6690.95
86_E119_E0.6670.95
31_L110_V0.6660.95
82_I120_L0.6620.94
26_F38_H0.6590.94
182_H185_G0.6580.94
121_L156_Y0.6580.94
157_L160_K0.6570.94
10_F23_F0.6570.94
32_M145_V0.6560.94
138_I171_V0.6550.94
189_R195_E0.6540.94
75_T103_C0.6540.94
171_V175_S0.6500.94
36_Y169_V0.6490.94
202_Y207_K0.6470.94
136_D140_Q0.6460.94
33_R144_G0.6400.93
168_Y199_L0.6340.93
39_N130_D0.6330.93
91_W94_A0.6330.93
120_L123_V0.6330.93
8_H41_D0.6280.93
60_V63_Y0.6240.92
133_Q174_W0.6220.92
57_M91_W0.6220.92
155_K158_A0.6220.92
129_L153_F0.6200.92
165_W198_E0.6190.92
25_T74_V0.6190.92
146_S149_R0.6180.92
126_L165_W0.6170.92
92_F123_V0.6170.92
206_G217_Y0.6150.92
134_M151_L0.6140.92
133_Q175_S0.6140.92
158_A193_N0.6070.91
138_I174_W0.6060.91
108_G154_A0.6050.91
106_T127_V0.6040.91
60_V100_I0.6020.91
92_F120_L0.6010.91
109_F146_S0.5970.91
90_D94_A0.5920.90
10_F56_L0.5850.90
210_W217_Y0.5820.90
60_V67_M0.5810.90
149_R152_E0.5740.89
172_P222_V0.5740.89
184_L197_I0.5730.89
111_R149_R0.5690.89
14_G22_R0.5680.89
139_H143_V0.5650.88
76_A81_A0.5630.88
189_R197_I0.5620.88
58_K98_E0.5620.88
53_V60_V0.5610.88
10_F59_E0.5600.88
25_T60_V0.5560.87
23_F63_Y0.5560.87
22_R26_F0.5550.87
12_S59_E0.5540.87
172_P202_Y0.5540.87
105_D167_R0.5540.87
202_Y210_W0.5530.87
169_V200_L0.5530.87
27_F79_G0.5530.87
120_L124_T0.5530.87
85_A119_E0.5520.87
13_C21_I0.5510.87
154_A164_V0.5490.87
8_H11_E0.5470.87
80_E107_N0.5460.87
121_L124_T0.5450.86
64_R98_E0.5430.86
56_L60_V0.5390.86
104_L120_L0.5380.86
54_E61_V0.5360.86
8_H28_Q0.5350.85
167_R200_L0.5340.85
168_Y188_T0.5330.85
67_M100_I0.5330.85
188_T194_V0.5330.85
34_C37_C0.5250.84
57_M67_M0.5230.84
129_L157_L0.5220.84
185_G197_I0.5210.84
30_C34_C0.5210.84
129_L166_I0.5200.84
145_V150_T0.5200.84
38_H205_L0.5140.83
87_F90_D0.5140.83
11_E15_T0.5120.83
53_V61_V0.5120.83
44_D47_G0.5110.83
86_E89_R0.5110.83
181_A199_L0.5110.83
9_S12_S0.5100.83
25_T91_W0.5100.83
117_I122_E0.5080.82
223_K226_K0.5030.82
119_E123_V0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3c8fA 1 0.9878 100 0.404 Contact Map
3canA 2 0.6341 100 0.574 Contact Map
3rfaA 1 0.9106 100 0.604 Contact Map
2yx0A 1 0.9187 100 0.615 Contact Map
2z2uA 1 0.8211 100 0.624 Contact Map
4njhA 2 0.7602 99.9 0.648 Contact Map
1tv8A 2 0.9024 99.9 0.654 Contact Map
1oltA 1 0.9797 99.9 0.665 Contact Map
4u0oB 1 0.9146 99.9 0.675 Contact Map
2qgqA 1 0.8008 99.9 0.684 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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