GREMLIN Database
PHOU - Phosphate-specific transport system accessory protein PhoU
UniProt: P0A9K7 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10735
Length: 241 (211)
Sequences: 4744 (3578)
Seq/√Len: 246.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_G143_L3.4461.00
141_D160_E3.3121.00
41_T45_N2.8991.00
33_E140_H2.6941.00
38_D53_R2.5231.00
144_D148_R2.5111.00
100_E104_D2.4951.00
62_N104_D2.4541.00
40_I132_G2.3471.00
61_V103_G2.3431.00
165_D204_D2.3311.00
157_I210_C2.2591.00
98_E208_N2.2161.00
38_D57_G2.1331.00
164_V203_G2.1251.00
58_D104_D2.1091.00
161_D165_D2.1041.00
24_Q71_A2.0591.00
37_S136_I2.0361.00
141_D156_R2.0251.00
32_V99_L1.9721.00
177_T181_E1.9101.00
35_Q61_V1.9071.00
134_H163_K1.8911.00
44_H129_E1.8481.00
98_E205_R1.8341.00
38_D50_L1.7941.00
52_K114_L1.7751.00
41_T50_L1.7671.00
58_D100_E1.7611.00
135_T164_V1.7431.00
165_D200_E1.7211.00
32_V61_V1.7141.00
127_S174_Q1.6941.00
92_I146_F1.6761.00
39_A106_A1.6731.00
62_N100_E1.6701.00
52_K56_E1.6581.00
141_D153_E1.6491.00
137_Q141_D1.6271.00
95_T209_I1.6141.00
160_E163_K1.5201.00
33_E37_S1.4741.00
58_D62_N1.4631.00
63_M67_A1.4621.00
161_D200_E1.4401.00
91_V216_Y1.4251.00
144_D153_E1.3981.00
179_M186_I1.3951.00
102_I202_I1.3951.00
27_T64_M1.3681.00
171_I196_A1.3661.00
36_L202_I1.3621.00
108_K198_S1.3541.00
135_T199_I1.3501.00
109_I195_C1.3491.00
25_V92_I1.3461.00
163_K166_Q1.3401.00
142_V206_C1.3341.00
138_M203_G1.3241.00
139_L206_C1.3061.00
142_V213_I1.3031.00
30_G33_E1.2921.00
48_S117_F1.2801.00
154_A214_F1.2741.00
150_D153_E1.2661.00
21_I75_I1.2631.00
200_E204_D1.2401.00
39_A113_A1.2391.00
57_G60_N1.2261.00
102_I139_L1.2261.00
161_D204_D1.2211.00
28_M65_E1.2151.00
55_I58_D1.2001.00
11_S79_R1.2001.00
152_D155_V1.1961.00
72_C89_V1.1821.00
156_R160_E1.1791.00
54_V110_C1.1731.00
74_R78_K1.1701.00
47_D50_L1.1701.00
134_H137_Q1.1651.00
75_I89_V1.1611.00
151_I217_V1.1571.00
208_N211_E1.1551.00
134_H160_E1.1541.00
35_Q54_V1.1491.00
33_E136_I1.1421.00
42_A54_V1.1351.00
155_V214_F1.1091.00
24_Q27_T1.0991.00
137_Q160_E1.0981.00
34_Q38_D1.0981.00
153_E156_R1.0921.00
53_R57_G1.0921.00
112_T191_T1.0891.00
24_Q67_A1.0871.00
88_L149_M1.0811.00
168_Y196_A1.0761.00
32_V103_G1.0731.00
148_R153_E1.0591.00
65_E94_K1.0551.00
35_Q57_G1.0541.00
162_K165_D1.0501.00
101_R201_R1.0431.00
51_A114_L1.0331.00
144_D147_A1.0321.00
145_A157_I1.0281.00
32_V102_I1.0251.00
58_D107_D1.0241.00
67_A71_A1.0241.00
17_E71_A1.0131.00
135_T206_C1.0111.00
115_E191_T1.0041.00
126_V129_E1.0031.00
175_L189_V1.0031.00
130_S163_K1.0021.00
73_V215_Y0.9991.00
134_H164_V0.9871.00
14_F75_I0.9811.00
127_S170_G0.9781.00
22_R88_L0.9661.00
72_C93_S0.9621.00
31_M61_V0.9521.00
48_S114_L0.9501.00
28_M64_M0.9491.00
31_M60_N0.9491.00
72_C96_I0.9471.00
73_V90_M0.9431.00
121_H124_L0.9431.00
55_I114_L0.9411.00
133_R137_Q0.9351.00
59_K62_N0.9331.00
138_M164_V0.9291.00
49_D52_K0.9281.00
200_E203_G0.9231.00
25_V96_I0.9221.00
42_A51_A0.9211.00
50_L53_R0.9141.00
131_L168_Y0.9141.00
21_I89_V0.9131.00
151_I214_F0.9021.00
113_A195_C0.8971.00
46_Q116_K0.8730.99
65_E96_I0.8690.99
94_K97_A0.8690.99
20_S71_A0.8650.99
12_G15_N0.8620.99
47_D51_A0.8610.99
22_R149_M0.8580.99
38_D41_T0.8550.99
177_T180_M0.8550.99
21_I72_C0.8510.99
28_M61_V0.8500.99
37_S140_H0.8500.99
166_Q170_G0.8480.99
130_S133_R0.8410.99
136_I140_H0.8400.99
30_G144_D0.8370.99
42_A113_A0.8300.99
48_S52_K0.8270.99
68_I97_A0.8270.99
30_G147_A0.8190.99
150_D154_A0.8120.99
140_H144_D0.8100.99
19_E23_T0.8070.99
15_N19_E0.8060.99
36_L106_A0.8060.99
26_M143_L0.8040.99
53_R56_E0.7960.99
145_A154_A0.7910.99
165_D203_G0.7870.99
14_F18_L0.7860.99
174_Q178_Y0.7850.99
179_M193_L0.7820.99
55_I110_C0.7810.99
54_V57_G0.7800.99
42_A46_Q0.7640.99
123_P126_V0.7590.99
98_E105_V0.7540.98
25_V95_T0.7530.98
100_E208_N0.7520.98
151_I155_V0.7510.98
173_R177_T0.7480.98
60_N63_M0.7450.98
94_K212_F0.7410.98
92_I95_T0.7400.98
71_A74_R0.7370.98
40_I199_I0.7350.98
206_C213_I0.7320.98
167_E170_G0.7290.98
90_M93_S0.7280.98
31_M64_M0.7270.98
127_S130_S0.7260.98
28_M96_I0.7260.98
77_A86_L0.7260.98
98_E212_F0.7250.98
134_H167_E0.7240.98
207_Q211_E0.7230.98
197_R205_R0.7220.98
172_V193_L0.7210.98
45_N50_L0.7210.98
19_E22_R0.7200.98
78_K83_A0.7190.98
27_T31_M0.7180.98
111_R169_E0.7170.98
112_T195_C0.7140.98
180_M184_R0.7140.98
126_V130_S0.7090.98
13_Q16_A0.7060.98
51_A110_C0.7060.98
131_L167_E0.6990.98
172_V176_M0.6930.97
32_V106_A0.6850.97
155_V159_R0.6820.97
105_V202_I0.6820.97
132_G199_I0.6810.97
138_M206_C0.6790.97
42_A50_L0.6780.97
35_Q39_A0.6760.97
105_V205_R0.6750.97
43_M195_C0.6710.97
105_V198_S0.6710.97
24_Q68_I0.6640.97
141_D144_D0.6640.97
102_I206_C0.6630.97
179_M189_V0.6610.97
190_L193_L0.6590.96
124_L184_R0.6590.96
37_S41_T0.6580.96
116_K188_S0.6540.96
39_A109_I0.6530.96
69_D94_K0.6520.96
157_I160_E0.6500.96
78_K81_P0.6480.96
174_Q177_T0.6470.96
127_S178_Y0.6470.96
130_S167_E0.6450.96
50_L54_V0.6450.96
69_D212_F0.6420.96
101_R205_R0.6420.96
182_D185_T0.6390.96
46_Q120_Q0.6370.96
96_I212_F0.6330.96
35_Q106_A0.6330.96
72_C76_I0.6310.96
131_L199_I0.6290.95
100_E103_G0.6260.95
162_K207_Q0.6120.95
168_Y172_V0.6120.95
138_M161_D0.6100.95
101_R208_N0.6070.94
132_G136_I0.6030.94
155_V218_K0.5990.94
40_I44_H0.5980.94
186_I189_V0.5980.94
51_A55_I0.5980.94
185_T189_V0.5960.94
35_Q103_G0.5920.94
88_L216_Y0.5920.94
106_A202_I0.5910.94
206_C210_C0.5900.94
124_L192_A0.5900.94
105_V168_Y0.5890.94
112_T198_S0.5870.93
95_T212_F0.5860.93
170_G173_R0.5840.93
152_D156_R0.5830.93
161_D203_G0.5820.93
20_S24_Q0.5800.93
42_A47_D0.5800.93
129_E133_R0.5770.93
40_I128_L0.5760.93
108_K112_T0.5750.93
46_Q117_F0.5750.93
22_R147_A0.5710.93
124_L175_L0.5680.92
130_S134_H0.5670.92
27_T30_G0.5660.92
180_M187_P0.5650.92
65_E69_D0.5640.92
77_A81_P0.5640.92
178_Y181_E0.5630.92
115_E119_Q0.5560.92
66_V69_D0.5540.91
122_Q125_L0.5530.91
158_Y211_E0.5530.91
33_E147_A0.5520.91
14_F17_E0.5520.91
72_C212_F0.5450.91
55_I107_D0.5450.91
34_Q37_S0.5420.90
69_D72_C0.5360.90
29_G99_L0.5350.90
135_T202_I0.5340.90
158_Y207_Q0.5340.90
139_L142_V0.5330.90
99_L139_L0.5330.90
83_A87_R0.5300.89
83_A86_L0.5270.89
81_P86_L0.5250.89
51_A58_D0.5250.89
203_G211_E0.5240.89
104_D208_N0.5240.89
121_H178_Y0.5230.89
93_S96_I0.5220.89
68_I96_I0.5180.88
123_P178_Y0.5160.88
194_F197_R0.5120.88
34_Q57_G0.5120.88
137_Q163_K0.5090.88
23_T27_T0.5090.88
17_E79_R0.5070.87
11_S82_T0.5070.87
108_K207_Q0.5020.87
51_A113_A0.5000.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q25A 2 0.8714 100 0.207 Contact Map
1sumB 2 0.9336 100 0.211 Contact Map
1xwmA 2 0.8797 100 0.221 Contact Map
2i0mA 2 0.8589 100 0.223 Contact Map
1t72A 5 0.8672 100 0.259 Contact Map
2oltA 5 0.8257 99.9 0.5 Contact Map
3l39A 3 0.8257 99.6 0.68 Contact Map
1vctA 3 0.7593 97.3 0.848 Contact Map
1krqA 6 0.6639 25.3 0.938 Contact Map
1eumA 6 0.6473 24.7 0.939 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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