GREMLIN Database
ALF1 - Fructose-bisphosphate aldolase class 1
UniProt: P0A991 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14062
Length: 350 (301)
Sequences: 408 (196)
Seq/√Len: 11.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
163_A195_V5.3641.00
149_L182_I5.0641.00
130_L197_V4.8281.00
319_L338_V4.7771.00
216_V242_E4.1520.99
277_A281_M4.0070.99
200_A228_A3.7760.99
327_S330_D3.5660.98
154_E188_R3.3210.97
34_D38_R3.0410.95
306_A314_G2.9240.94
87_A256_D2.7470.92
248_K257_D2.7060.91
164_V185_A2.5390.88
113_Y155_Q2.5180.88
80_S247_Y2.4680.87
59_T63_A2.4490.86
188_R191_E2.4430.86
82_G274_Y2.4380.86
51_R55_T2.4280.86
131_V161_A2.3910.85
47_P120_S2.3890.85
258_R262_K2.2890.82
58_N64_G2.2780.82
148_T176_R2.2570.81
256_D261_S2.2440.81
48_A55_T2.2100.80
236_V279_C2.1960.79
182_I198_L2.1580.78
270_D273_R2.1230.77
240_M301_D2.0920.76
264_T270_D2.0750.75
40_M284_A2.0720.75
296_E330_D2.0600.75
131_V151_A2.0150.73
111_S159_M1.9770.71
36_V54_Q1.9350.70
52_N162_V1.9170.69
240_M272_V1.9100.69
299_L333_K1.9090.69
258_R261_S1.8830.67
335_I338_V1.8560.66
113_Y151_A1.8530.66
229_A236_V1.8380.66
329_A333_K1.8230.65
140_S144_T1.7530.62
52_N195_V1.7490.61
48_A157_F1.7420.61
95_K122_R1.7270.60
240_M314_G1.6900.59
200_A236_V1.6740.58
108_C197_V1.6600.57
155_Q158_N1.6560.57
104_A335_I1.6550.57
57_Y284_A1.6480.57
151_A155_Q1.6390.56
56_L108_C1.6110.55
104_A338_V1.5910.54
40_M118_S1.5800.53
104_A319_L1.5740.53
257_D261_S1.5600.52
151_A232_G1.5460.52
229_A279_C1.5390.51
95_K99_E1.5330.51
152_S187_E1.5140.50
55_T162_V1.4940.49
52_N55_T1.4750.48
217_S243_N1.4610.48
44_N48_A1.4450.47
146_D181_E1.4400.47
117_A232_G1.4330.46
133_L153_V1.3930.44
45_R49_V1.3850.44
319_L335_I1.3810.44
297_T326_K1.3780.44
208_K215_H1.3770.44
287_I315_M1.3680.43
247_Y257_D1.3670.43
48_A131_V1.3640.43
272_V312_A1.3580.43
87_A258_R1.3560.43
113_Y183_S1.3260.41
146_D174_E1.3250.41
113_Y152_S1.3130.41
300_S326_K1.3080.40
45_R234_D1.2910.40
187_E232_G1.2810.39
53_M326_K1.2800.39
173_E332_V1.2780.39
151_A180_E1.2740.39
35_Y38_R1.2520.38
208_K246_G1.2520.38
220_L247_Y1.2370.37
205_S292_A1.2170.36
44_N117_A1.2080.36
131_V149_L1.2050.36
118_S283_R1.2040.36
144_T176_R1.1960.35
44_N232_G1.1950.35
35_Y57_Y1.1900.35
146_D178_Q1.1760.34
40_M57_Y1.1750.34
216_V292_A1.1640.34
145_Y173_E1.1590.34
200_A241_A1.1580.34
166_A186_F1.1450.33
36_V41_I1.1370.33
56_L345_S1.1340.32
59_T108_C1.1270.32
248_K261_S1.1250.32
216_V243_N1.1190.32
46_P113_Y1.1180.32
48_A51_R1.1130.32
295_G298_D1.1100.31
48_A158_N1.1100.31
256_D259_V1.1060.31
151_A187_E1.0990.31
200_A229_A1.0990.31
68_L107_N1.0950.31
90_L329_A1.0900.31
108_C304_R1.0890.31
296_E334_L1.0830.30
39_V113_Y1.0480.29
104_A292_A1.0470.29
228_A232_G1.0420.29
99_E328_M1.0410.29
56_L99_E1.0400.29
47_P345_S1.0270.28
259_V272_V1.0260.28
207_F221_T1.0190.28
53_M195_V1.0190.28
98_V111_S1.0100.27
258_R264_T1.0090.27
57_Y315_M1.0090.27
216_V344_D1.0030.27
323_A335_I1.0000.27
168_I236_V0.9890.27
55_T188_R0.9830.26
173_E205_S0.9810.26
305_T310_K0.9810.26
240_M305_T0.9790.26
229_A280_Y0.9740.26
99_E103_E0.9710.26
228_A236_V0.9700.26
301_D305_T0.9700.26
256_D262_K0.9670.26
164_V182_I0.9670.26
67_Y278_N0.9660.26
57_Y68_L0.9630.26
117_A158_N0.9570.25
61_R283_R0.9540.25
205_S344_D0.9530.25
151_A183_S0.9460.25
228_A241_A0.9450.25
35_Y281_M0.9440.25
155_Q159_M0.9440.25
44_N155_Q0.9400.25
323_A327_S0.9360.25
46_P49_V0.9220.24
58_N195_V0.9200.24
33_H262_K0.9180.24
287_I334_L0.9100.24
325_K338_V0.9060.24
205_S208_K0.9040.23
62_L65_T0.9000.23
135_H230_T0.8990.23
58_N262_K0.8990.23
133_L197_V0.8940.23
48_A228_A0.8920.23
225_N279_C0.8900.23
152_S188_R0.8860.23
155_Q180_E0.8860.23
149_L185_A0.8820.23
83_A179_I0.8800.23
197_V276_L0.8800.23
274_Y277_A0.8730.22
106_C339_Q0.8700.22
35_Y42_D0.8650.22
37_D126_R0.8560.22
102_I299_L0.8540.22
145_Y257_D0.8530.22
202_L215_H0.8500.22
56_L68_L0.8500.22
329_A345_S0.8470.22
202_L232_G0.8460.22
46_P155_Q0.8450.21
57_Y272_V0.8410.21
326_K336_N0.8400.21
54_Q262_K0.8400.21
107_N210_D0.8380.21
108_C130_L0.8370.21
148_T180_E0.8350.21
240_M312_A0.8340.21
173_E206_A0.8340.21
113_Y131_V0.8330.21
218_A222_G0.8300.21
299_L303_V0.8290.21
148_T163_A0.8270.21
162_V195_V0.8250.21
40_M52_N0.8210.21
40_M232_G0.8200.21
260_Y277_A0.8190.21
48_A155_Q0.8190.21
58_N68_L0.8170.21
289_S318_I0.8170.21
48_A52_N0.8160.21
217_S258_R0.8160.21
136_N176_R0.8150.21
148_T179_I0.8140.20
99_E261_S0.8110.20
98_V119_V0.8030.20
37_D44_N0.7990.20
68_L149_L0.7960.20
40_M43_N0.7940.20
35_Y243_N0.7920.20
48_A232_G0.7910.20
174_E178_Q0.7840.20
275_Q285_G0.7830.20
103_E126_R0.7780.19
140_S178_Q0.7780.19
55_T236_V0.7770.19
65_T208_K0.7750.19
265_S271_L0.7710.19
35_Y315_M0.7690.19
49_V155_Q0.7660.19
82_G99_E0.7660.19
258_R265_S0.7660.19
48_A81_A0.7640.19
205_S294_G0.7610.19
56_L154_E0.7590.19
90_L164_V0.7590.19
83_A319_L0.7580.19
178_Q342_Y0.7560.19
48_A120_S0.7560.19
151_A173_E0.7560.19
184_A187_E0.7550.19
205_S229_A0.7530.19
158_N232_G0.7490.18
240_M306_A0.7480.18
37_D236_V0.7390.18
42_D46_P0.7380.18
175_S179_I0.7380.18
65_T209_K0.7340.18
36_V131_V0.7340.18
296_E300_S0.7320.18
159_M242_E0.7320.18
98_V331_G0.7270.18
133_L164_V0.7240.18
86_A259_V0.7230.18
81_A304_R0.7210.18
70_I108_C0.7200.18
143_N173_E0.7130.17
297_T301_D0.7130.17
191_E281_M0.7120.17
80_S259_V0.7110.17
51_R162_V0.7100.17
304_R334_L0.7080.17
145_Y216_V0.7050.17
310_K314_G0.7030.17
40_M197_V0.7010.17
154_E183_S0.7000.17
164_V303_V0.7000.17
52_N157_F0.6990.17
37_D45_R0.6970.17
133_L185_A0.6950.17
113_Y158_N0.6940.17
142_P186_F0.6890.17
153_V185_A0.6880.17
43_N118_S0.6870.17
34_D164_V0.6870.17
146_D330_D0.6870.17
106_C137_E0.6840.17
259_V262_K0.6840.17
73_V111_S0.6830.17
91_Y96_N0.6800.17
259_V264_T0.6770.16
300_S341_V0.6710.16
57_Y118_S0.6710.16
87_A283_R0.6700.16
145_Y217_S0.6690.16
48_A77_V0.6680.16
81_A209_K0.6630.16
128_P165_G0.6630.16
108_C216_V0.6590.16
96_N99_E0.6540.16
209_K215_H0.6530.16
54_Q58_N0.6510.16
40_M157_F0.6460.16
285_G315_M0.6450.16
204_N322_K0.6450.16
133_L208_K0.6440.16
71_L235_I0.6430.16
300_S344_D0.6410.16
336_N340_D0.6390.15
72_P100_L0.6380.15
210_D258_R0.6360.15
99_E163_A0.6340.15
55_T59_T0.6340.15
102_I217_S0.6330.15
243_N248_K0.6330.15
105_G344_D0.6290.15
146_D227_L0.6260.15
154_E162_V0.6260.15
290_G321_R0.6250.15
290_G316_G0.6250.15
316_G321_R0.6250.15
290_G320_G0.6250.15
316_G320_G0.6250.15
115_V136_N0.6240.15
87_A332_V0.6240.15
291_G321_R0.6230.15
291_G316_G0.6230.15
291_G320_G0.6230.15
117_A228_A0.6210.15
50_L121_R0.6200.15
134_N199_W0.6180.15
117_A159_M0.6160.15
122_R126_R0.6140.15
281_M344_D0.6130.15
55_T131_V0.6120.15
35_Y283_R0.6120.15
48_A200_A0.6090.15
177_R273_R0.6080.15
91_Y278_N0.6080.15
218_A265_S0.6050.15
72_P124_A0.6030.15
131_V229_A0.6030.15
39_V47_P0.6030.15
79_H132_K0.6020.15
78_E132_K0.6000.15
69_S339_Q0.5990.14
42_D216_V0.5960.14
127_I341_V0.5960.14
116_L162_V0.5960.14
38_R283_R0.5960.14
242_E292_A0.5950.14
77_V158_N0.5950.14
178_Q181_E0.5920.14
84_S168_I0.5920.14
134_N165_G0.5910.14
38_R115_V0.5910.14
180_E183_S0.5900.14
198_L278_N0.5900.14
38_R301_D0.5880.14
55_T209_K0.5880.14
46_P131_V0.5850.14
46_P194_M0.5840.14
43_N95_K0.5840.14
232_G328_M0.5820.14
36_V39_V0.5810.14
296_E326_K0.5770.14
155_Q297_T0.5770.14
115_V119_V0.5740.14
158_N326_K0.5740.14
261_S264_T0.5720.14
147_Q314_G0.5720.14
120_S312_A0.5720.14
99_E345_S0.5710.14
54_Q326_K0.5690.14
87_A269_I0.5680.14
180_E228_A0.5680.14
36_V157_F0.5660.14
35_Y249_A0.5660.14
90_L99_E0.5650.14
56_L59_T0.5630.14
55_T163_A0.5620.14
110_A165_G0.5620.14
43_N50_L0.5610.14
265_S270_D0.5610.14
257_D264_T0.5600.14
111_S249_A0.5580.14
82_G259_V0.5570.13
147_Q166_A0.5570.13
37_D56_L0.5560.13
154_E192_L0.5550.13
276_L287_I0.5540.13
48_A113_Y0.5540.13
248_K262_K0.5540.13
268_P304_R0.5530.13
144_T205_S0.5520.13
258_R344_D0.5500.13
294_G327_S0.5490.13
68_L345_S0.5490.13
305_T314_G0.5480.13
179_I231_I0.5470.13
40_M265_S0.5450.13
34_D301_D0.5440.13
79_H134_N0.5420.13
83_A168_I0.5410.13
175_S262_K0.5410.13
154_E179_I0.5400.13
149_L184_A0.5390.13
80_S220_L0.5370.13
271_L314_G0.5370.13
36_V338_V0.5360.13
38_R41_I0.5360.13
36_V279_C0.5350.13
101_A199_W0.5340.13
39_V131_V0.5340.13
200_A232_G0.5340.13
197_V279_C0.5330.13
87_A249_A0.5330.13
59_T301_D0.5330.13
45_R186_F0.5320.13
44_N190_H0.5320.13
299_L330_D0.5310.13
187_E257_D0.5300.13
174_E329_A0.5290.13
61_R86_A0.5280.13
323_A338_V0.5270.13
77_V131_V0.5260.13
165_G199_W0.5260.13
47_P247_Y0.5260.13
300_S329_A0.5260.13
83_A202_L0.5260.13
82_G133_L0.5250.13
163_A272_V0.5240.13
319_L323_A0.5230.13
239_K289_S0.5210.13
300_S334_L0.5210.13
163_A228_A0.5200.13
76_G80_S0.5180.13
218_A310_K0.5180.13
203_R318_I0.5150.13
268_P273_R0.5150.13
242_E344_D0.5140.13
78_E134_N0.5140.13
118_S284_A0.5140.13
306_A317_L0.5130.13
99_E164_V0.5120.13
56_L163_A0.5120.13
54_Q164_V0.5110.12
137_E179_I0.5110.12
232_G261_S0.5100.12
184_A296_E0.5100.12
52_N200_A0.5090.12
44_N115_V0.5090.12
192_L196_T0.5060.12
208_K271_L0.5060.12
77_V150_Y0.5050.12
181_E294_G0.5050.12
165_G282_G0.5050.12
333_K336_N0.5050.12
100_L289_S0.5050.12
101_A128_P0.5040.12
137_E175_S0.5040.12
101_A134_N0.5030.12
187_E211_G0.5030.12
36_V40_M0.5020.12
68_L210_D0.5020.12
57_Y108_C0.5010.12
38_R42_D0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3iv3A 2 0.8286 100 0.613 Contact Map
3fokA 3 0.7543 100 0.617 Contact Map
4mozA 5 0.8257 100 0.621 Contact Map
1to3A 2 0.7543 100 0.701 Contact Map
3glcA 6 0.76 100 0.774 Contact Map
1w8sA 4 0.7171 100 0.779 Contact Map
2qjgA 6 0.7514 100 0.81 Contact Map
3oa3A 2 0.68 99.9 0.835 Contact Map
3r12A 2 0.7086 99.8 0.863 Contact Map
3ndoA 2 0.6257 99.8 0.867 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0093 seconds.