ASPG1 - ECOLI - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

ASPG1 - L-asparaginase 1

UniProt:	P0A962	Sequences: MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10045
Length:	338 (317)
Sequences:	3449 (2521)
Seq/√Len:	141.6

ECOLI - ASPG1 ASPG2
BACSU - ASPG1 ASPG2
HALSA - GATD
SULSO - GATD

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
101_L	114_V	4.428	1.00
227_R	259_E	4.241	1.00
5_S	48_D	4.107	1.00
216_T	240_R	3.867	1.00
97_T	114_V	3.716	1.00
52_H	74_D	3.489	1.00
105_L	112_V	3.369	1.00
149_L	161_T	3.272	1.00
227_R	256_E	3.025	1.00
318_D	321_T	2.965	1.00
321_T	324_K	2.960	1.00
7_Y	82_Y	2.898	1.00
58_M	67_D	2.740	1.00
9_A	54_Y	2.567	1.00
312_L	325_A	2.514	1.00
50_T	82_Y	2.405	1.00
156_Y	174_S	2.371	1.00
236_A	309_L	2.362	1.00
230_L	260_A	2.351	1.00
5_S	50_T	2.326	1.00
65_P	220_G	2.264	1.00
86_V	139_A	2.232	1.00
240_R	302_V	2.214	1.00
150_F	155_L	2.214	1.00
75_I	87_I	2.214	1.00
213_G	234_V	2.174	1.00
10_Y	16_G	2.168	1.00
258_Q	262_D	2.142	1.00
270_L	297_G	2.139	1.00
79_Y	110_K	2.137	1.00
156_Y	161_T	2.121	1.00
164_A	173_A	2.110	1.00
79_Y	107_N	2.021	1.00
79_Y	85_F	1.999	1.00
107_N	110_K	1.992	1.00
7_Y	78_H	1.992	1.00
155_L	181_L	1.957	1.00
268_V	326_M	1.928	1.00
72_A	104_M	1.925	1.00
94_M	149_L	1.899	1.00
269_N	294_V	1.877	1.00
134_N	153_N	1.877	1.00
62_D	249_Q	1.870	1.00
312_L	322_I	1.819	1.00
9_A	74_D	1.804	1.00
223_A	227_R	1.791	1.00
9_A	52_H	1.791	1.00
68_W	219_P	1.780	1.00
64_T	67_D	1.768	1.00
138_V	155_L	1.764	1.00
184_G	187_I	1.760	1.00
241_S	269_N	1.740	1.00
268_V	297_G	1.739	1.00
102_S	159_N	1.725	1.00
319_T	323_R	1.708	1.00
55_T	67_D	1.685	1.00
319_T	322_I	1.680	1.00
31_L	51_I	1.664	1.00
54_Y	74_D	1.656	1.00
65_P	310_H	1.631	1.00
227_R	231_R	1.625	1.00
115_T	151_F	1.619	1.00
256_E	259_E	1.584	1.00
68_W	307_T	1.583	1.00
164_A	171_A	1.571	1.00
268_V	309_L	1.556	1.00
214_V	302_V	1.554	1.00
63_M	67_D	1.551	1.00
134_N	150_F	1.550	1.00
225_V	229_F	1.540	1.00
89_H	97_T	1.525	1.00
223_A	256_E	1.524	1.00
100_A	104_M	1.523	1.00
209_P	235_K	1.502	1.00
76_K	80_D	1.495	1.00
20_S	25_I	1.491	1.00
211_P	222_S	1.457	1.00
75_I	112_V	1.442	1.00
266_V	322_I	1.436	1.00
323_R	327_S	1.431	1.00
74_D	77_A	1.430	1.00
226_V	260_A	1.425	1.00
138_V	150_F	1.409	1.00
78_H	81_D	1.403	1.00
133_L	137_Y	1.392	1.00
74_D	78_H	1.375	1.00
259_E	263_R	1.353	1.00
32_Q	36_A	1.331	1.00
69_Q	104_M	1.327	1.00
109_G	144_I	1.326	1.00
24_Y	27_V	1.303	1.00
211_P	235_K	1.294	1.00
237_L	260_A	1.287	1.00
165_H	275_S	1.287	1.00
215_V	226_V	1.284	1.00
10_Y	31_L	1.278	1.00
68_W	100_A	1.276	1.00
211_P	224_D	1.270	1.00
166_A	271_T	1.268	1.00
30_H	33_R	1.264	1.00
146_E	157_R	1.251	1.00
270_L	302_V	1.249	1.00
184_G	188_R	1.246	1.00
154_R	170_D	1.235	1.00
258_Q	292_A	1.228	1.00
81_D	110_K	1.215	1.00
73_E	206_P	1.211	1.00
72_A	76_K	1.205	1.00
226_V	256_E	1.203	1.00
10_Y	53_E	1.202	1.00
52_H	78_H	1.193	1.00
228_N	232_Q	1.188	1.00
126_S	129_Q	1.183	1.00
287_N	291_H	1.159	0.99
58_M	63_M	1.154	0.99
29_G	121_L	1.132	0.99
130_I	134_N	1.130	0.99
216_T	302_V	1.128	0.99
73_E	77_A	1.117	0.99
266_V	295_I	1.114	0.99
86_V	140_A	1.104	0.99
65_P	68_W	1.104	0.99
226_V	253_F	1.098	0.99
86_V	136_L	1.098	0.99
4_K	142_Y	1.093	0.99
210_Q	314_S	1.092	0.99
6_I	83_D	1.091	0.99
212_I	313_L	1.090	0.99
316_E	325_A	1.088	0.99
67_D	70_H	1.087	0.99
211_P	234_V	1.086	0.99
217_I	248_P	1.083	0.99
71_I	87_I	1.078	0.99
17_M	24_Y	1.074	0.99
230_L	265_I	1.068	0.99
229_F	234_V	1.065	0.99
94_M	116_G	1.055	0.99
309_L	322_I	1.051	0.99
5_S	83_D	1.051	0.99
87_I	114_V	1.049	0.99
215_V	229_F	1.044	0.99
312_L	316_E	1.030	0.99
75_I	85_F	1.029	0.99
223_A	259_E	1.027	0.99
261_S	267_V	1.024	0.99
41_F	136_L	1.023	0.99
190_L	193_P	1.023	0.99
239_L	257_L	1.020	0.99
182_E	190_L	1.017	0.99
55_T	71_I	1.015	0.99
148_T	180_L	1.015	0.99
213_G	229_F	1.007	0.99
61_S	246_N	1.006	0.99
116_G	151_F	0.998	0.98
47_P	140_A	0.990	0.98
66_E	70_H	0.986	0.98
85_F	110_K	0.983	0.98
93_T	242_Y	0.978	0.98
19_R	23_G	0.972	0.98
261_S	293_G	0.972	0.98
31_L	34_Q	0.969	0.98
91_T	116_G	0.966	0.98
39_P	133_L	0.961	0.98
15_I	128_G	0.961	0.98
247_A	257_L	0.954	0.98
96_Y	272_Q	0.954	0.98
156_Y	179_P	0.948	0.98
257_L	294_V	0.948	0.98
18_Q	33_R	0.946	0.98
224_D	228_N	0.936	0.98
217_I	239_L	0.936	0.98
317_L	321_T	0.930	0.98
119_I	169_F	0.928	0.98
306_L	310_H	0.924	0.97
99_S	103_F	0.923	0.97
272_Q	303_E	0.923	0.97
94_M	114_V	0.922	0.97
288_A	291_H	0.921	0.97
246_N	286_G	0.919	0.97
50_T	78_H	0.915	0.97
95_A	303_E	0.914	0.97
9_A	87_I	0.908	0.97
162_T	302_V	0.899	0.97
48_D	83_D	0.895	0.97
38_M	41_F	0.882	0.97
102_S	158_G	0.880	0.97
69_Q	73_E	0.879	0.97
91_T	163_K	0.877	0.97
166_A	277_K	0.875	0.96
6_I	140_A	0.875	0.96
76_K	206_P	0.874	0.96
150_F	183_A	0.874	0.96
160_R	304_A	0.867	0.96
217_I	253_F	0.858	0.96
269_N	280_M	0.850	0.96
65_P	219_P	0.847	0.96
84_G	111_P	0.847	0.96
53_E	57_L	0.847	0.96
40_E	129_Q	0.844	0.96
167_D	244_V	0.842	0.96
189_R	193_P	0.841	0.96
160_R	299_D	0.841	0.96
76_K	204_V	0.840	0.96
46_M	133_L	0.839	0.96
7_Y	52_H	0.837	0.95
26_P	29_G	0.830	0.95
95_A	272_Q	0.829	0.95
138_V	183_A	0.827	0.95
145_N	194_P	0.827	0.95
47_P	136_L	0.822	0.95
305_T	326_M	0.819	0.95
125_R	130_I	0.815	0.95
270_L	305_T	0.810	0.95
113_I	148_T	0.809	0.95
92_D	243_G	0.805	0.94
8_V	132_L	0.803	0.94
8_V	88_L	0.802	0.94
280_M	290_A	0.800	0.94
99_S	159_N	0.799	0.94
224_D	232_Q	0.798	0.94
25_I	28_S	0.798	0.94
231_R	259_E	0.794	0.94
316_E	321_T	0.792	0.94
257_L	261_S	0.790	0.94
226_V	257_L	0.789	0.94
154_R	179_P	0.789	0.94
146_E	180_L	0.788	0.94
261_S	294_V	0.786	0.94
165_H	168_G	0.785	0.94
221_I	253_F	0.784	0.94
321_T	325_A	0.780	0.93
9_A	78_H	0.778	0.93
261_S	292_A	0.778	0.93
212_I	238_I	0.776	0.93
230_L	256_E	0.772	0.93
165_H	276_G	0.772	0.93
11_T	89_H	0.771	0.93
164_A	274_M	0.770	0.93
230_L	233_P	0.769	0.93
200_G	203_I	0.769	0.93
289_L	294_V	0.766	0.93
77_A	80_D	0.766	0.93
148_T	155_L	0.765	0.93
191_N	194_P	0.761	0.93
16_G	88_L	0.760	0.93
99_S	307_T	0.760	0.93
59_D	62_D	0.757	0.92
183_A	188_R	0.757	0.92
221_I	248_P	0.757	0.92
102_S	157_R	0.755	0.92
87_I	101_L	0.753	0.92
157_R	180_L	0.752	0.92
163_K	243_G	0.752	0.92
130_I	169_F	0.749	0.92
152_N	170_D	0.749	0.92
217_I	247_A	0.748	0.92
259_E	262_D	0.748	0.92
300_M	308_K	0.746	0.92
113_I	139_A	0.745	0.92
302_V	305_T	0.740	0.92
166_A	273_C	0.738	0.91
236_A	305_T	0.737	0.91
230_L	237_L	0.731	0.91
75_I	79_Y	0.731	0.91
217_I	242_Y	0.730	0.91
78_H	85_F	0.729	0.91
295_I	326_M	0.729	0.91
189_R	194_P	0.727	0.91
82_Y	110_K	0.723	0.91
162_T	301_T	0.721	0.90
121_L	126_S	0.720	0.90
190_L	194_P	0.719	0.90
247_A	253_F	0.718	0.90
154_R	173_A	0.715	0.90
8_V	136_L	0.712	0.90
242_Y	248_P	0.711	0.90
78_H	82_Y	0.711	0.90
261_S	264_G	0.710	0.90
70_H	74_D	0.710	0.90
230_L	263_R	0.708	0.90
82_Y	85_F	0.707	0.90
255_Q	258_Q	0.706	0.89
238_I	268_V	0.705	0.89
235_K	265_I	0.704	0.89
280_M	296_G	0.703	0.89
55_T	70_H	0.702	0.89
103_F	308_K	0.702	0.89
16_G	57_L	0.700	0.89
75_I	105_L	0.700	0.89
106_E	205_H	0.697	0.89
235_K	317_L	0.696	0.89
266_V	319_T	0.696	0.89
316_E	319_T	0.695	0.89
301_T	304_A	0.694	0.89
92_D	246_N	0.693	0.89
121_L	128_G	0.688	0.88
215_V	225_V	0.687	0.88
255_Q	259_E	0.685	0.88
7_Y	50_T	0.685	0.88
95_A	161_T	0.684	0.88
4_K	83_D	0.684	0.88
318_D	325_A	0.683	0.88
279_N	282_G	0.683	0.88
320_E	324_K	0.680	0.88
127_D	163_K	0.679	0.88
72_A	100_A	0.677	0.87
221_I	226_V	0.676	0.87
38_M	136_L	0.676	0.87
157_R	160_R	0.675	0.87
15_I	88_L	0.673	0.87
217_I	221_I	0.671	0.87
175_P	275_S	0.671	0.87
61_S	242_Y	0.671	0.87
103_F	311_Y	0.669	0.87
17_M	27_V	0.668	0.87
167_D	279_N	0.667	0.87
145_N	148_T	0.667	0.87
160_R	308_K	0.664	0.86
8_V	34_Q	0.663	0.86
98_A	149_L	0.662	0.86
79_Y	105_L	0.661	0.86
19_R	122_A	0.656	0.86
215_V	221_I	0.656	0.86
20_S	26_P	0.651	0.85
229_F	233_P	0.649	0.85
68_W	97_T	0.649	0.85
154_R	182_E	0.649	0.85
135_A	150_F	0.648	0.85
210_Q	235_K	0.644	0.85
197_H	200_G	0.642	0.85
311_Y	315_Q	0.642	0.85
114_V	158_G	0.640	0.84
65_P	69_Q	0.640	0.84
205_H	315_Q	0.638	0.84
256_E	260_A	0.637	0.84
244_V	279_N	0.636	0.84
45_E	48_D	0.636	0.84
99_S	308_K	0.635	0.84
115_T	135_A	0.633	0.84
170_D	173_A	0.632	0.84
15_I	117_S	0.629	0.83
11_T	71_I	0.629	0.83
96_Y	306_L	0.628	0.83
223_A	252_A	0.627	0.83
106_E	203_I	0.623	0.83
23_G	28_S	0.622	0.83
113_I	138_V	0.622	0.83
213_G	222_S	0.620	0.82
151_F	156_Y	0.618	0.82
120_P	123_E	0.618	0.82
219_P	303_E	0.617	0.82
49_F	86_V	0.615	0.82
70_H	77_A	0.613	0.82
244_V	269_N	0.612	0.82
76_K	106_E	0.612	0.82
288_A	292_A	0.611	0.82
243_G	246_N	0.611	0.82
297_G	305_T	0.606	0.81
98_A	161_T	0.604	0.81
304_A	308_K	0.603	0.81
318_D	324_K	0.603	0.81
260_A	265_I	0.602	0.81
254_L	292_A	0.602	0.81
236_A	270_L	0.602	0.81
39_P	47_P	0.602	0.81
17_M	61_S	0.602	0.81
103_F	159_N	0.602	0.81
236_A	266_V	0.601	0.81
118_Q	171_A	0.601	0.81
289_L	292_A	0.601	0.81
46_M	140_A	0.600	0.80
17_M	26_P	0.600	0.80
155_L	188_R	0.600	0.80
62_D	285_T	0.599	0.80
236_A	313_L	0.598	0.80
223_A	226_V	0.597	0.80
118_Q	243_G	0.597	0.80
88_L	132_L	0.595	0.80
103_F	304_A	0.594	0.80
10_Y	17_M	0.594	0.80
260_A	266_V	0.594	0.80
239_L	247_A	0.594	0.80
297_G	326_M	0.593	0.80
72_A	105_L	0.590	0.79
71_I	74_D	0.589	0.79
116_G	163_K	0.588	0.79
174_S	178_P	0.588	0.79
73_E	207_I	0.587	0.79
260_A	267_V	0.587	0.79
176_N	299_D	0.580	0.78
241_S	247_A	0.576	0.78
280_M	286_G	0.576	0.78
19_R	123_E	0.573	0.77
72_A	204_V	0.572	0.77
185_I	188_R	0.571	0.77
9_A	75_I	0.569	0.77
213_G	221_I	0.569	0.77
99_S	158_G	0.569	0.77
73_E	210_Q	0.568	0.77
32_Q	50_T	0.568	0.77
59_D	158_G	0.566	0.76
29_G	53_E	0.566	0.76
23_G	26_P	0.565	0.76
54_Y	220_G	0.565	0.76
68_W	101_L	0.565	0.76
59_D	94_M	0.563	0.76
18_Q	27_V	0.562	0.76
167_D	277_K	0.562	0.76
79_Y	82_Y	0.561	0.76
177_L	193_P	0.560	0.76
30_H	34_Q	0.560	0.76
254_L	258_Q	0.560	0.76
66_E	263_R	0.560	0.76
162_T	274_M	0.559	0.76
237_L	257_L	0.558	0.75
7_Y	75_I	0.556	0.75
76_K	107_N	0.555	0.75
195_A	200_G	0.551	0.75
225_V	234_V	0.550	0.74
233_P	240_R	0.550	0.74
118_Q	163_K	0.544	0.74
277_K	298_A	0.541	0.73
24_Y	123_E	0.541	0.73
144_I	148_T	0.540	0.73
138_V	148_T	0.540	0.73
228_N	231_R	0.537	0.73
266_V	313_L	0.537	0.73
67_D	71_I	0.536	0.73
10_Y	51_I	0.534	0.72
148_T	181_L	0.534	0.72
38_M	47_P	0.533	0.72
134_N	183_A	0.533	0.72
12_G	15_I	0.533	0.72
196_P	199_E	0.533	0.72
69_Q	207_I	0.533	0.72
234_V	265_I	0.531	0.72
76_K	105_L	0.531	0.72
135_A	138_V	0.531	0.72
190_L	298_A	0.530	0.72
63_M	97_T	0.529	0.72
227_R	230_L	0.528	0.71
35_L	39_P	0.527	0.71
16_G	31_L	0.527	0.71
322_I	326_M	0.526	0.71
225_V	228_N	0.525	0.71
68_W	89_H	0.525	0.71
98_A	114_V	0.524	0.71
246_N	289_L	0.523	0.71
60_S	89_H	0.523	0.71
72_A	101_L	0.523	0.71
218_Y	310_H	0.522	0.71
118_Q	169_F	0.520	0.70
91_T	156_Y	0.520	0.70
278_V	296_G	0.520	0.70
69_Q	100_A	0.520	0.70
60_S	245_G	0.519	0.70
32_Q	37_L	0.518	0.70
184_G	244_V	0.517	0.70
304_A	307_T	0.517	0.70
137_Y	141_N	0.517	0.70
119_I	125_R	0.515	0.70
151_F	171_A	0.515	0.70
11_T	14_T	0.512	0.69
300_M	304_A	0.511	0.69
46_M	49_F	0.511	0.69
162_T	303_E	0.510	0.69
92_D	283_Y	0.509	0.69
124_L	153_N	0.508	0.68
231_R	263_R	0.507	0.68
55_T	58_M	0.507	0.68
316_E	323_R	0.504	0.68
226_V	258_Q	0.504	0.68
24_Y	126_S	0.502	0.68
214_V	306_L	0.501	0.67
195_A	198_G	0.501	0.67
118_Q	244_V	0.500	0.67

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2himA	4	0.9704	100	0.04	Contact Map
4q0mA	2	0.9645	100	0.065	Contact Map
1wlsA	2	0.9675	100	0.066	Contact Map
2d6fA	2	0.9793	100	0.069	Contact Map
1zq1A	2	0.9822	100	0.07	Contact Map
4r8kA	3	0.9852	100	0.073	Contact Map
1wsaA	4	0.926	100	0.106	Contact Map
2wltA	4	0.932	100	0.108	Contact Map
1nnsA	4	0.9408	100	0.11	Contact Map
3nxkA	4	0.9349	100	0.113	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0106 seconds.