GREMLIN Database
ASPG1 - L-asparaginase 1
UniProt: P0A962 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10045
Length: 338 (317)
Sequences: 3449 (2521)
Seq/√Len: 141.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
101_L114_V4.4281.00
227_R259_E4.2411.00
5_S48_D4.1071.00
216_T240_R3.8671.00
97_T114_V3.7161.00
52_H74_D3.4891.00
105_L112_V3.3691.00
149_L161_T3.2721.00
227_R256_E3.0251.00
318_D321_T2.9651.00
321_T324_K2.9601.00
7_Y82_Y2.8981.00
58_M67_D2.7401.00
9_A54_Y2.5671.00
312_L325_A2.5141.00
50_T82_Y2.4051.00
156_Y174_S2.3711.00
236_A309_L2.3621.00
230_L260_A2.3511.00
5_S50_T2.3261.00
65_P220_G2.2641.00
86_V139_A2.2321.00
240_R302_V2.2141.00
150_F155_L2.2141.00
75_I87_I2.2141.00
213_G234_V2.1741.00
10_Y16_G2.1681.00
258_Q262_D2.1421.00
270_L297_G2.1391.00
79_Y110_K2.1371.00
156_Y161_T2.1211.00
164_A173_A2.1101.00
79_Y107_N2.0211.00
79_Y85_F1.9991.00
107_N110_K1.9921.00
7_Y78_H1.9921.00
155_L181_L1.9571.00
268_V326_M1.9281.00
72_A104_M1.9251.00
94_M149_L1.8991.00
269_N294_V1.8771.00
134_N153_N1.8771.00
62_D249_Q1.8701.00
312_L322_I1.8191.00
9_A74_D1.8041.00
223_A227_R1.7911.00
9_A52_H1.7911.00
68_W219_P1.7801.00
64_T67_D1.7681.00
138_V155_L1.7641.00
184_G187_I1.7601.00
241_S269_N1.7401.00
268_V297_G1.7391.00
102_S159_N1.7251.00
319_T323_R1.7081.00
55_T67_D1.6851.00
319_T322_I1.6801.00
31_L51_I1.6641.00
54_Y74_D1.6561.00
65_P310_H1.6311.00
227_R231_R1.6251.00
115_T151_F1.6191.00
256_E259_E1.5841.00
68_W307_T1.5831.00
164_A171_A1.5711.00
268_V309_L1.5561.00
214_V302_V1.5541.00
63_M67_D1.5511.00
134_N150_F1.5501.00
225_V229_F1.5401.00
89_H97_T1.5251.00
223_A256_E1.5241.00
100_A104_M1.5231.00
209_P235_K1.5021.00
76_K80_D1.4951.00
20_S25_I1.4911.00
211_P222_S1.4571.00
75_I112_V1.4421.00
266_V322_I1.4361.00
323_R327_S1.4311.00
74_D77_A1.4301.00
226_V260_A1.4251.00
138_V150_F1.4091.00
78_H81_D1.4031.00
133_L137_Y1.3921.00
74_D78_H1.3751.00
259_E263_R1.3531.00
32_Q36_A1.3311.00
69_Q104_M1.3271.00
109_G144_I1.3261.00
24_Y27_V1.3031.00
211_P235_K1.2941.00
237_L260_A1.2871.00
165_H275_S1.2871.00
215_V226_V1.2841.00
10_Y31_L1.2781.00
68_W100_A1.2761.00
211_P224_D1.2701.00
166_A271_T1.2681.00
30_H33_R1.2641.00
146_E157_R1.2511.00
270_L302_V1.2491.00
184_G188_R1.2461.00
154_R170_D1.2351.00
258_Q292_A1.2281.00
81_D110_K1.2151.00
73_E206_P1.2111.00
72_A76_K1.2051.00
226_V256_E1.2031.00
10_Y53_E1.2021.00
52_H78_H1.1931.00
228_N232_Q1.1881.00
126_S129_Q1.1831.00
287_N291_H1.1590.99
58_M63_M1.1540.99
29_G121_L1.1320.99
130_I134_N1.1300.99
216_T302_V1.1280.99
73_E77_A1.1170.99
266_V295_I1.1140.99
86_V140_A1.1040.99
65_P68_W1.1040.99
226_V253_F1.0980.99
86_V136_L1.0980.99
4_K142_Y1.0930.99
210_Q314_S1.0920.99
6_I83_D1.0910.99
212_I313_L1.0900.99
316_E325_A1.0880.99
67_D70_H1.0870.99
211_P234_V1.0860.99
217_I248_P1.0830.99
71_I87_I1.0780.99
17_M24_Y1.0740.99
230_L265_I1.0680.99
229_F234_V1.0650.99
94_M116_G1.0550.99
309_L322_I1.0510.99
5_S83_D1.0510.99
87_I114_V1.0490.99
215_V229_F1.0440.99
312_L316_E1.0300.99
75_I85_F1.0290.99
223_A259_E1.0270.99
261_S267_V1.0240.99
41_F136_L1.0230.99
190_L193_P1.0230.99
239_L257_L1.0200.99
182_E190_L1.0170.99
55_T71_I1.0150.99
148_T180_L1.0150.99
213_G229_F1.0070.99
61_S246_N1.0060.99
116_G151_F0.9980.98
47_P140_A0.9900.98
66_E70_H0.9860.98
85_F110_K0.9830.98
93_T242_Y0.9780.98
19_R23_G0.9720.98
261_S293_G0.9720.98
31_L34_Q0.9690.98
91_T116_G0.9660.98
39_P133_L0.9610.98
15_I128_G0.9610.98
247_A257_L0.9540.98
96_Y272_Q0.9540.98
156_Y179_P0.9480.98
257_L294_V0.9480.98
18_Q33_R0.9460.98
224_D228_N0.9360.98
217_I239_L0.9360.98
317_L321_T0.9300.98
119_I169_F0.9280.98
306_L310_H0.9240.97
99_S103_F0.9230.97
272_Q303_E0.9230.97
94_M114_V0.9220.97
288_A291_H0.9210.97
246_N286_G0.9190.97
50_T78_H0.9150.97
95_A303_E0.9140.97
9_A87_I0.9080.97
162_T302_V0.8990.97
48_D83_D0.8950.97
38_M41_F0.8820.97
102_S158_G0.8800.97
69_Q73_E0.8790.97
91_T163_K0.8770.97
166_A277_K0.8750.96
6_I140_A0.8750.96
76_K206_P0.8740.96
150_F183_A0.8740.96
160_R304_A0.8670.96
217_I253_F0.8580.96
269_N280_M0.8500.96
65_P219_P0.8470.96
84_G111_P0.8470.96
53_E57_L0.8470.96
40_E129_Q0.8440.96
167_D244_V0.8420.96
189_R193_P0.8410.96
160_R299_D0.8410.96
76_K204_V0.8400.96
46_M133_L0.8390.96
7_Y52_H0.8370.95
26_P29_G0.8300.95
95_A272_Q0.8290.95
138_V183_A0.8270.95
145_N194_P0.8270.95
47_P136_L0.8220.95
305_T326_M0.8190.95
125_R130_I0.8150.95
270_L305_T0.8100.95
113_I148_T0.8090.95
92_D243_G0.8050.94
8_V132_L0.8030.94
8_V88_L0.8020.94
280_M290_A0.8000.94
99_S159_N0.7990.94
224_D232_Q0.7980.94
25_I28_S0.7980.94
231_R259_E0.7940.94
316_E321_T0.7920.94
257_L261_S0.7900.94
226_V257_L0.7890.94
154_R179_P0.7890.94
146_E180_L0.7880.94
261_S294_V0.7860.94
165_H168_G0.7850.94
221_I253_F0.7840.94
321_T325_A0.7800.93
9_A78_H0.7780.93
261_S292_A0.7780.93
212_I238_I0.7760.93
230_L256_E0.7720.93
165_H276_G0.7720.93
11_T89_H0.7710.93
164_A274_M0.7700.93
230_L233_P0.7690.93
200_G203_I0.7690.93
289_L294_V0.7660.93
77_A80_D0.7660.93
148_T155_L0.7650.93
191_N194_P0.7610.93
16_G88_L0.7600.93
99_S307_T0.7600.93
59_D62_D0.7570.92
183_A188_R0.7570.92
221_I248_P0.7570.92
102_S157_R0.7550.92
87_I101_L0.7530.92
157_R180_L0.7520.92
163_K243_G0.7520.92
130_I169_F0.7490.92
152_N170_D0.7490.92
217_I247_A0.7480.92
259_E262_D0.7480.92
300_M308_K0.7460.92
113_I139_A0.7450.92
302_V305_T0.7400.92
166_A273_C0.7380.91
236_A305_T0.7370.91
230_L237_L0.7310.91
75_I79_Y0.7310.91
217_I242_Y0.7300.91
78_H85_F0.7290.91
295_I326_M0.7290.91
189_R194_P0.7270.91
82_Y110_K0.7230.91
162_T301_T0.7210.90
121_L126_S0.7200.90
190_L194_P0.7190.90
247_A253_F0.7180.90
154_R173_A0.7150.90
8_V136_L0.7120.90
242_Y248_P0.7110.90
78_H82_Y0.7110.90
261_S264_G0.7100.90
70_H74_D0.7100.90
230_L263_R0.7080.90
82_Y85_F0.7070.90
255_Q258_Q0.7060.89
238_I268_V0.7050.89
235_K265_I0.7040.89
280_M296_G0.7030.89
55_T70_H0.7020.89
103_F308_K0.7020.89
16_G57_L0.7000.89
75_I105_L0.7000.89
106_E205_H0.6970.89
235_K317_L0.6960.89
266_V319_T0.6960.89
316_E319_T0.6950.89
301_T304_A0.6940.89
92_D246_N0.6930.89
121_L128_G0.6880.88
215_V225_V0.6870.88
255_Q259_E0.6850.88
7_Y50_T0.6850.88
95_A161_T0.6840.88
4_K83_D0.6840.88
318_D325_A0.6830.88
279_N282_G0.6830.88
320_E324_K0.6800.88
127_D163_K0.6790.88
72_A100_A0.6770.87
221_I226_V0.6760.87
38_M136_L0.6760.87
157_R160_R0.6750.87
15_I88_L0.6730.87
217_I221_I0.6710.87
175_P275_S0.6710.87
61_S242_Y0.6710.87
103_F311_Y0.6690.87
17_M27_V0.6680.87
167_D279_N0.6670.87
145_N148_T0.6670.87
160_R308_K0.6640.86
8_V34_Q0.6630.86
98_A149_L0.6620.86
79_Y105_L0.6610.86
19_R122_A0.6560.86
215_V221_I0.6560.86
20_S26_P0.6510.85
229_F233_P0.6490.85
68_W97_T0.6490.85
154_R182_E0.6490.85
135_A150_F0.6480.85
210_Q235_K0.6440.85
197_H200_G0.6420.85
311_Y315_Q0.6420.85
114_V158_G0.6400.84
65_P69_Q0.6400.84
205_H315_Q0.6380.84
256_E260_A0.6370.84
244_V279_N0.6360.84
45_E48_D0.6360.84
99_S308_K0.6350.84
115_T135_A0.6330.84
170_D173_A0.6320.84
15_I117_S0.6290.83
11_T71_I0.6290.83
96_Y306_L0.6280.83
223_A252_A0.6270.83
106_E203_I0.6230.83
23_G28_S0.6220.83
113_I138_V0.6220.83
213_G222_S0.6200.82
151_F156_Y0.6180.82
120_P123_E0.6180.82
219_P303_E0.6170.82
49_F86_V0.6150.82
70_H77_A0.6130.82
244_V269_N0.6120.82
76_K106_E0.6120.82
288_A292_A0.6110.82
243_G246_N0.6110.82
297_G305_T0.6060.81
98_A161_T0.6040.81
304_A308_K0.6030.81
318_D324_K0.6030.81
260_A265_I0.6020.81
254_L292_A0.6020.81
236_A270_L0.6020.81
39_P47_P0.6020.81
17_M61_S0.6020.81
103_F159_N0.6020.81
236_A266_V0.6010.81
118_Q171_A0.6010.81
289_L292_A0.6010.81
46_M140_A0.6000.80
17_M26_P0.6000.80
155_L188_R0.6000.80
62_D285_T0.5990.80
236_A313_L0.5980.80
223_A226_V0.5970.80
118_Q243_G0.5970.80
88_L132_L0.5950.80
103_F304_A0.5940.80
10_Y17_M0.5940.80
260_A266_V0.5940.80
239_L247_A0.5940.80
297_G326_M0.5930.80
72_A105_L0.5900.79
71_I74_D0.5890.79
116_G163_K0.5880.79
174_S178_P0.5880.79
73_E207_I0.5870.79
260_A267_V0.5870.79
176_N299_D0.5800.78
241_S247_A0.5760.78
280_M286_G0.5760.78
19_R123_E0.5730.77
72_A204_V0.5720.77
185_I188_R0.5710.77
9_A75_I0.5690.77
213_G221_I0.5690.77
99_S158_G0.5690.77
73_E210_Q0.5680.77
32_Q50_T0.5680.77
59_D158_G0.5660.76
29_G53_E0.5660.76
23_G26_P0.5650.76
54_Y220_G0.5650.76
68_W101_L0.5650.76
59_D94_M0.5630.76
18_Q27_V0.5620.76
167_D277_K0.5620.76
79_Y82_Y0.5610.76
177_L193_P0.5600.76
30_H34_Q0.5600.76
254_L258_Q0.5600.76
66_E263_R0.5600.76
162_T274_M0.5590.76
237_L257_L0.5580.75
7_Y75_I0.5560.75
76_K107_N0.5550.75
195_A200_G0.5510.75
225_V234_V0.5500.74
233_P240_R0.5500.74
118_Q163_K0.5440.74
277_K298_A0.5410.73
24_Y123_E0.5410.73
144_I148_T0.5400.73
138_V148_T0.5400.73
228_N231_R0.5370.73
266_V313_L0.5370.73
67_D71_I0.5360.73
10_Y51_I0.5340.72
148_T181_L0.5340.72
38_M47_P0.5330.72
134_N183_A0.5330.72
12_G15_I0.5330.72
196_P199_E0.5330.72
69_Q207_I0.5330.72
234_V265_I0.5310.72
76_K105_L0.5310.72
135_A138_V0.5310.72
190_L298_A0.5300.72
63_M97_T0.5290.72
227_R230_L0.5280.71
35_L39_P0.5270.71
16_G31_L0.5270.71
322_I326_M0.5260.71
225_V228_N0.5250.71
68_W89_H0.5250.71
98_A114_V0.5240.71
246_N289_L0.5230.71
60_S89_H0.5230.71
72_A101_L0.5230.71
218_Y310_H0.5220.71
118_Q169_F0.5200.70
91_T156_Y0.5200.70
278_V296_G0.5200.70
69_Q100_A0.5200.70
60_S245_G0.5190.70
32_Q37_L0.5180.70
184_G244_V0.5170.70
304_A307_T0.5170.70
137_Y141_N0.5170.70
119_I125_R0.5150.70
151_F171_A0.5150.70
11_T14_T0.5120.69
300_M304_A0.5110.69
46_M49_F0.5110.69
162_T303_E0.5100.69
92_D283_Y0.5090.69
124_L153_N0.5080.68
231_R263_R0.5070.68
55_T58_M0.5070.68
316_E323_R0.5040.68
226_V258_Q0.5040.68
24_Y126_S0.5020.68
214_V306_L0.5010.67
195_A198_G0.5010.67
118_Q244_V0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2himA 4 0.9704 100 0.04 Contact Map
4q0mA 2 0.9645 100 0.065 Contact Map
1wlsA 2 0.9675 100 0.066 Contact Map
2d6fA 2 0.9793 100 0.069 Contact Map
1zq1A 2 0.9822 100 0.07 Contact Map
4r8kA 3 0.9852 100 0.073 Contact Map
1wsaA 4 0.926 100 0.106 Contact Map
2wltA 4 0.932 100 0.108 Contact Map
1nnsA 4 0.9408 100 0.11 Contact Map
3nxkA 4 0.9349 100 0.113 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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