GREMLIN Database
TRMB - tRNA (guanine-N(7)-)-methyltransferase
UniProt: P0A8I5 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11779
Length: 239 (200)
Sequences: 3597 (2370)
Seq/√Len: 167.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
136_R170_Q4.1771.00
83_K110_E4.1691.00
199_K236_E3.4301.00
85_R90_F2.8971.00
132_D168_K2.8041.00
55_D58_A2.7521.00
107_A115_L2.6121.00
138_V165_V2.5731.00
132_D167_S2.4711.00
182_E186_E2.4561.00
62_R89_D2.3621.00
67_T88_Q2.3511.00
127_H164_L2.3431.00
140_L143_P2.2871.00
78_L92_G2.1911.00
177_M188_M2.1681.00
169_L173_G2.1541.00
163_E195_I2.0851.00
140_L175_F2.0811.00
140_L157_V2.0571.00
42_W46_G2.0291.00
159_V191_V2.0111.00
80_A84_D1.9521.00
59_L116_R1.9521.00
49_F52_D1.8781.00
72_F92_G1.8631.00
137_M174_V1.8561.00
187_H191_V1.8501.00
67_T139_Q1.8261.00
67_T137_M1.8151.00
182_E229_G1.7731.00
216_V221_Q1.7711.00
193_S235_F1.7481.00
65_P136_R1.7401.00
56_F66_V1.6401.00
57_P63_E1.6371.00
65_P89_D1.5631.00
166_K197_G1.5531.00
195_I198_Y1.5391.00
82_A112_L1.5371.00
85_R88_Q1.5341.00
76_A221_Q1.5251.00
64_A89_D1.4911.00
103_C117_V1.4751.00
97_S119_C1.4661.00
135_L168_K1.4451.00
80_A83_K1.4411.00
64_A87_E1.4411.00
41_Y108_H1.4411.00
60_F89_D1.4311.00
66_V89_D1.4291.00
157_V188_M1.4161.00
135_L138_V1.4021.00
221_Q225_R1.3961.00
107_A117_V1.3851.00
70_I122_A1.3791.00
166_K198_Y1.3481.00
203_E206_D1.3471.00
127_H167_S1.3431.00
54_L134_S1.3221.00
53_M131_P1.3091.00
182_E231_W1.3001.00
152_N183_P1.2991.00
68_L135_L1.2951.00
198_Y203_E1.2921.00
209_P212_A1.2901.00
93_I122_A1.2871.00
183_P186_E1.2851.00
205_N208_V1.2771.00
151_H154_R1.2571.00
175_F192_M1.2501.00
126_L164_L1.2441.00
62_R66_V1.2341.00
83_K112_L1.2201.00
174_V236_E1.1881.00
138_V169_L1.1881.00
176_H232_D1.1861.00
130_I134_S1.1811.00
159_V194_S1.1571.00
91_L130_I1.1531.00
140_L156_I1.1531.00
174_V234_M1.1461.00
79_V106_S1.1391.00
146_W151_H1.1381.00
120_H129_M1.1381.00
133_N170_Q1.1331.00
77_S139_Q1.1211.00
198_Y202_S1.1051.00
189_L235_F1.0961.00
124_E128_K1.0851.00
179_T185_A1.0781.00
70_I138_V1.0771.00
162_A191_V1.0751.00
152_N187_H1.0701.00
137_M176_H1.0440.99
133_N171_L1.0400.99
106_S110_E1.0390.99
92_G103_C1.0340.99
166_K195_I1.0280.99
56_F130_I1.0160.99
216_V224_H1.0080.99
160_P163_E0.9990.99
68_L138_V0.9960.99
201_L236_E0.9910.99
222_R226_L0.9880.99
156_I161_F0.9860.99
204_S208_V0.9850.99
163_E166_K0.9790.99
74_M102_A0.9650.99
131_P134_S0.9640.99
224_H230_V0.9490.99
68_L93_I0.9400.99
60_F91_L0.9220.99
164_L167_S0.9210.99
149_A152_N0.9090.98
147_H152_N0.9040.98
112_L115_L0.9020.98
77_S81_M0.8990.98
100_V117_V0.8870.98
42_W48_E0.8860.98
63_E89_D0.8840.98
228_H231_W0.8820.98
159_V163_E0.8780.98
81_M85_R0.8740.98
56_F134_S0.8700.98
79_V103_C0.8680.98
67_T81_M0.8670.98
88_Q137_M0.8630.98
37_A41_Y0.8620.98
146_W154_R0.8600.98
81_M215_P0.8590.98
107_A112_L0.8540.98
158_Q187_H0.8530.98
79_V112_L0.8520.98
219_F222_R0.8510.98
223_G228_H0.8510.98
55_D134_S0.8500.98
188_M233_L0.8470.97
223_G226_L0.8450.97
47_V59_L0.8440.97
105_A109_E0.8420.97
192_M198_Y0.8410.97
80_A215_P0.8390.97
47_V52_D0.8310.97
208_V213_S0.8170.97
42_W154_R0.8080.97
67_T90_F0.8080.97
82_A114_N0.8030.96
93_I125_V0.7990.96
81_M139_Q0.7960.96
41_Y44_V0.7870.96
200_N203_E0.7850.96
61_G64_A0.7810.96
40_N108_H0.7780.96
148_K151_H0.7760.96
95_V123_V0.7730.96
37_A105_A0.7700.96
57_P66_V0.7640.95
72_F94_E0.7570.95
100_V119_C0.7550.95
93_I118_M0.7540.95
197_G203_E0.7540.95
79_V107_A0.7500.95
76_A106_S0.7410.95
152_N158_Q0.7400.94
206_D209_P0.7370.94
66_V136_R0.7340.94
197_G201_L0.7320.94
198_Y201_L0.7290.94
192_M233_L0.7270.94
88_Q136_R0.7270.94
205_N209_P0.7250.94
74_M218_K0.7240.94
159_V195_I0.7240.94
47_V50_S0.7150.93
204_S207_Y0.7080.93
166_K171_L0.7050.93
72_F103_C0.7050.93
190_E194_S0.7020.93
41_Y45_M0.7020.93
214_R232_D0.7010.93
140_L165_V0.6950.92
183_P187_H0.6940.92
189_L200_N0.6930.92
78_L115_L0.6910.92
76_A218_K0.6790.92
102_A106_S0.6720.91
177_M184_Y0.6690.91
96_H99_G0.6690.91
102_A105_A0.6670.91
201_L206_D0.6640.91
156_I165_V0.6640.91
164_L168_K0.6610.90
201_L234_M0.6590.90
48_E51_E0.6570.90
150_R153_K0.6550.90
101_G105_A0.6540.90
220_E224_H0.6520.90
70_I140_L0.6500.90
77_S141_F0.6490.90
182_E228_H0.6460.89
193_S200_N0.6420.89
98_P102_A0.6410.89
84_D213_S0.6410.89
106_S109_E0.6410.89
146_W150_R0.6380.89
72_F78_L0.6370.89
105_A108_H0.6370.89
41_Y46_G0.6360.89
95_V119_C0.6350.89
45_M50_S0.6330.88
138_V173_G0.6320.88
79_V110_E0.6310.88
94_E122_A0.6300.88
221_Q224_H0.6300.88
67_T85_R0.6280.88
200_N207_Y0.6250.88
68_L130_I0.6240.88
144_D148_K0.6240.88
76_A80_A0.6230.88
157_V165_V0.6220.88
162_A175_F0.6150.87
142_F230_V0.6110.87
188_M235_F0.6100.87
159_V187_H0.6100.87
192_M235_F0.6020.86
98_P158_Q0.6010.86
65_P88_Q0.6000.86
107_A111_G0.5980.86
189_L206_D0.5940.85
149_A190_E0.5920.85
190_E201_L0.5920.85
47_V51_E0.5870.84
133_N168_K0.5850.84
170_Q173_G0.5850.84
162_A195_I0.5840.84
197_G202_S0.5830.84
123_V164_L0.5810.84
144_D153_K0.5800.84
81_M90_F0.5800.84
79_V83_K0.5790.84
37_A45_M0.5790.84
90_F139_Q0.5770.83
140_L177_M0.5760.83
120_H125_V0.5740.83
160_P164_L0.5720.83
95_V121_D0.5710.83
37_A40_N0.5700.83
197_G200_N0.5690.83
143_P184_Y0.5680.82
94_E97_S0.5670.82
99_G181_W0.5640.82
150_R154_R0.5620.82
90_F114_N0.5610.82
96_H102_A0.5590.81
49_F128_K0.5570.81
200_N234_M0.5560.81
77_S218_K0.5540.81
208_V211_P0.5520.81
213_S234_M0.5520.81
84_D215_P0.5510.81
78_L90_F0.5480.80
144_D154_R0.5460.80
52_D129_M0.5450.80
38_L41_Y0.5450.80
72_F117_V0.5440.80
58_A61_G0.5430.80
40_N43_P0.5420.79
210_R213_S0.5400.79
66_V135_L0.5390.79
99_G104_L0.5390.79
163_E197_G0.5390.79
188_M191_V0.5370.79
202_S205_N0.5320.78
45_M107_A0.5310.78
39_E42_W0.5310.78
122_A126_L0.5300.78
125_V128_K0.5270.78
189_L193_S0.5270.78
224_H228_H0.5270.78
130_I168_K0.5260.77
190_E193_S0.5250.77
42_W45_M0.5240.77
126_L168_K0.5220.77
111_G133_N0.5190.77
152_N191_V0.5160.76
178_A185_A0.5150.76
178_A214_R0.5140.76
61_G89_D0.5140.76
146_W153_K0.5130.76
226_L229_G0.5110.75
147_H151_H0.5070.75
158_Q162_A0.5060.75
96_H121_D0.5060.75
82_A115_L0.5060.75
208_V212_A0.5050.75
163_E167_S0.5050.75
186_E231_W0.5030.74
46_G50_S0.5000.74
78_L139_Q0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ckkA 1 0.7657 100 0.549 Contact Map
3dxyA 1 0.8536 100 0.556 Contact Map
2vdvE 2 0.8117 100 0.56 Contact Map
2fcaA 2 0.8368 100 0.573 Contact Map
1yzhA 1 0.841 100 0.607 Contact Map
4rwzA 1 0.7197 99.6 0.76 Contact Map
3p2eA 1 0.7573 99.6 0.764 Contact Map
1i9gA 3 0.9121 99.5 0.778 Contact Map
2pwyA 3 0.8954 99.5 0.781 Contact Map
1o54A 4 0.8745 99.5 0.782 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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