GREMLIN Database
TPIS - Triosephosphate isomerase
UniProt: P0A858 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11015
Length: 255 (244)
Sequences: 4614 (3008)
Seq/√Len: 192.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_A62_G3.2551.00
135_E176_K3.1771.00
184_Q226_D3.1521.00
139_T186_V2.7941.00
138_K141_E2.6191.00
44_E78_T2.2891.00
238_K241_A2.2831.00
13_N71_S2.2721.00
148_D193_H2.2341.00
143_C186_V2.1501.00
140_E189_F2.0971.00
125_L150_V2.0901.00
113_F125_L2.0591.00
116_L121_L2.0341.00
133_E142_V2.0061.00
106_D145_R1.9751.00
155_G158_A1.9301.00
74_F101_Y1.9281.00
3_H37_A1.9221.00
215_A241_A1.9221.00
60_M89_Q1.9011.00
213_V230_A1.8911.00
38_V246_V1.8891.00
106_D149_A1.8771.00
144_A189_F1.8761.00
133_E138_K1.8471.00
5_L163_V1.8441.00
51_K61_L1.8401.00
66_V80_A1.8381.00
131_E135_E1.7921.00
70_L118_E1.7711.00
83_L88_A1.7671.00
131_E176_K1.7501.00
199_A203_E1.7441.00
37_A206_I1.6791.00
181_A224_Q1.6701.00
66_V116_L1.6621.00
7_M124_V1.6621.00
63_A83_L1.6621.00
7_M39_A1.6521.00
183_A221_L1.6291.00
109_I125_L1.6251.00
117_K162_A1.5981.00
68_L111_K1.5941.00
45_M78_T1.5771.00
180_P220_E1.5711.00
14_G18_M1.5691.00
5_L39_A1.5681.00
41_A92_I1.5511.00
194_I205_V1.5451.00
69_N111_K1.5321.00
140_E185_A1.5271.00
127_I143_C1.5271.00
130_T169_V1.5241.00
91_I116_L1.5071.00
130_T133_E1.5001.00
198_D201_I1.4931.00
29_E243_A1.4791.00
48_D52_R1.4641.00
66_V93_I1.4511.00
69_N74_F1.4381.00
122_T161_G1.4371.00
188_K192_D1.4341.00
54_A61_L1.4301.00
21_E25_N1.3891.00
65_N102_H1.3881.00
6_V222_F1.3881.00
195_A199_A1.3761.00
110_A114_A1.3661.00
105_S108_L1.3631.00
213_V222_F1.3551.00
19_V50_A1.3511.00
42_P64_Q1.3461.00
92_I124_V1.3361.00
160_E204_Q1.3291.00
23_V50_A1.3191.00
144_A193_H1.3141.00
2_R228_D1.2801.00
67_D102_H1.2721.00
70_L119_Q1.2691.00
183_A227_I1.2651.00
65_N77_E1.2621.00
65_N112_K1.2601.00
19_V46_Y1.2571.00
158_A161_G1.2541.00
68_L108_L1.2521.00
114_A118_E1.2401.00
144_A148_D1.2311.00
42_P50_A1.2251.00
191_R207_I1.2241.00
115_V118_E1.2231.00
177_S221_L1.2041.00
183_A224_Q1.1971.00
100_T130_T1.1951.00
5_L208_Q1.1881.00
15_S18_M1.1861.00
134_N182_Q1.1831.00
90_Y122_T1.1801.00
39_A90_Y1.1771.00
77_E102_H1.1751.00
127_I164_I1.1741.00
189_F193_H1.1631.00
67_D115_V1.1631.00
91_I121_L1.1511.00
80_A115_V1.1501.00
127_I147_I1.1491.00
22_L239_A1.1491.00
63_A91_I1.1391.00
15_S85_D1.1271.00
184_Q225_P1.1241.00
14_G19_V1.1241.00
80_A119_Q1.1231.00
180_P183_A1.1181.00
216_S241_A1.1101.00
110_A153_T1.1071.00
25_N240_D1.1061.00
41_A124_V1.0991.00
195_A202_A1.0981.00
133_E145_R1.0931.00
130_T142_V1.0741.00
142_V169_V1.0721.00
47_I63_A1.0721.00
115_V119_Q1.0671.00
43_P46_Y1.0631.00
70_L115_V1.0611.00
218_A222_F1.0581.00
17_H85_D1.0461.00
4_P222_F1.0461.00
182_Q185_A1.0441.00
68_L102_H1.0411.00
195_A203_E1.0311.00
114_A153_T1.0301.00
66_V91_I1.0221.00
70_L81_A1.0221.00
218_A245_I1.0191.00
93_I113_F1.0151.00
54_A59_I1.0081.00
132_A136_A1.0071.00
244_V247_K0.9971.00
222_F230_A0.9971.00
110_A150_V0.9941.00
214_N241_A0.9890.99
193_H196_K0.9890.99
16_R49_M0.9830.99
213_V232_V0.9810.99
81_A119_Q0.9770.99
24_S28_K0.9740.99
52_R55_E0.9730.99
141_E144_A0.9700.99
47_I61_L0.9700.99
192_D196_K0.9570.99
12_L79_S0.9550.99
4_P206_I0.9520.99
192_D195_A0.9470.99
49_M52_R0.9450.99
181_A185_A0.9420.99
37_A60_M0.9380.99
5_L206_I0.9290.99
127_I169_V0.9260.99
24_S53_E0.9240.99
224_Q227_I0.9160.99
189_F192_D0.9100.99
184_Q224_Q0.9080.99
221_L227_I0.9070.99
89_Q122_T0.9070.99
20_H53_E0.9050.99
16_R46_Y0.9030.99
16_R48_D0.9020.99
154_Q158_A0.8980.99
191_R226_D0.8970.99
200_N204_Q0.8950.99
60_M90_Y0.8920.99
139_T143_C0.8840.99
22_L26_L0.8780.99
84_K119_Q0.8720.99
27_R57_S0.8650.99
43_P82_M0.8650.99
159_F162_A0.8620.99
15_S46_Y0.8590.99
117_K161_G0.8590.99
20_H24_S0.8590.99
71_S81_A0.8580.99
147_I190_I0.8570.99
45_M48_D0.8540.98
63_A88_A0.8490.98
100_T169_V0.8400.98
17_H21_E0.8390.98
55_E58_H0.8350.98
145_R149_A0.8330.98
90_Y123_P0.8260.98
51_K86_I0.8250.98
134_N185_A0.8250.98
22_L50_A0.8210.98
26_L239_A0.8200.98
113_F162_A0.8200.98
107_E111_K0.8200.98
113_F150_V0.8190.98
80_A116_L0.8140.98
140_E144_A0.8140.98
23_V54_A0.8130.98
25_N29_E0.8120.98
148_D152_K0.8110.98
61_L88_A0.8110.98
159_F205_V0.8080.98
24_S27_R0.8060.98
188_K226_D0.7970.98
45_M86_I0.7960.98
46_Y82_M0.7960.98
23_V53_E0.7940.98
144_A196_K0.7940.98
122_T163_V0.7940.98
69_N102_H0.7930.98
93_I116_L0.7910.97
103_K108_L0.7890.97
222_F248_A0.7860.97
127_I190_I0.7800.97
113_F117_K0.7790.97
190_I207_I0.7770.97
14_G71_S0.7760.97
25_N239_A0.7730.97
243_A247_K0.7720.97
50_A61_L0.7720.97
221_L230_A0.7680.97
6_V218_A0.7670.97
181_A220_E0.7650.97
14_G22_L0.7620.97
48_D86_I0.7570.97
77_E98_R0.7560.97
240_D244_V0.7510.97
84_K120_G0.7490.97
218_A230_A0.7420.96
20_H49_M0.7400.96
107_E149_A0.7390.96
232_V245_I0.7380.96
131_E174_T0.7380.96
19_V49_M0.7350.96
184_Q188_K0.7340.96
164_I190_I0.7300.96
19_V22_L0.7220.96
154_Q162_A0.7210.96
188_K225_P0.7200.96
150_V153_T0.7180.96
159_F201_I0.7180.96
215_A244_V0.7120.95
114_A158_A0.7100.95
7_M41_A0.7010.95
195_A198_D0.6970.95
241_A244_V0.6950.95
29_E240_D0.6950.95
191_R228_D0.6950.95
45_M82_M0.6930.95
39_A124_V0.6800.94
143_C190_I0.6780.94
66_V119_Q0.6780.94
27_R53_E0.6780.94
218_A248_A0.6780.94
149_A152_K0.6750.94
62_G90_Y0.6750.94
90_Y163_V0.6660.93
19_V23_V0.6600.93
37_A122_T0.6580.93
141_E145_R0.6580.93
27_R56_G0.6570.93
38_V42_P0.6550.93
116_L123_P0.6520.93
140_E186_V0.6520.93
51_K89_Q0.6520.93
51_K54_A0.6480.92
108_L111_K0.6480.92
143_C189_F0.6460.92
16_R85_D0.6440.92
32_G247_K0.6420.92
180_P224_Q0.6410.92
123_P159_F0.6410.92
27_R55_E0.6400.92
178_A221_L0.6370.92
18_M21_E0.6340.92
80_A121_L0.6340.92
214_N217_N0.6340.92
111_K153_T0.6240.91
110_A154_Q0.6230.91
76_G98_R0.6220.91
178_A183_A0.6210.91
39_A62_G0.6190.91
78_T82_M0.6140.90
117_K154_Q0.6090.90
26_L40_I0.6090.90
39_A163_V0.6090.90
147_I193_H0.6070.90
43_P78_T0.6020.89
49_M53_E0.6020.89
4_P228_D0.6010.89
198_D202_A0.5990.89
51_K87_G0.5960.89
67_D108_L0.5930.89
125_L147_I0.5900.89
3_H206_I0.5890.88
187_H227_I0.5860.88
26_L243_A0.5850.88
125_L162_A0.5850.88
142_V145_R0.5820.88
140_E143_C0.5820.88
40_I50_A0.5790.88
9_N231_L0.5760.87
46_Y85_D0.5750.87
38_V242_F0.5720.87
14_G46_Y0.5720.87
90_Y124_V0.5710.87
174_T177_S0.5670.87
29_E247_K0.5670.87
151_L159_F0.5640.86
187_H218_A0.5630.86
216_S220_E0.5620.86
66_V83_L0.5610.86
26_L59_I0.5600.86
23_V27_R0.5580.86
113_F154_Q0.5580.86
28_K31_A0.5520.85
234_G237_L0.5500.85
232_V236_S0.5500.85
50_A54_A0.5490.85
217_N221_L0.5480.85
33_V36_C0.5470.85
22_L40_I0.5450.84
67_D101_Y0.5440.84
197_V201_I0.5430.84
220_E223_A0.5400.84
25_N28_K0.5390.84
74_F102_H0.5370.83
219_A223_A0.5370.83
68_L112_K0.5360.83
109_I150_V0.5350.83
243_A246_V0.5350.83
127_I186_V0.5340.83
51_K88_A0.5320.83
33_V58_H0.5320.83
20_H27_R0.5300.83
66_V121_L0.5260.82
105_S109_I0.5260.82
54_A57_S0.5230.82
83_L86_I0.5230.82
23_V59_I0.5230.82
93_I109_I0.5200.81
21_E24_S0.5160.81
20_H52_R0.5160.81
164_I194_I0.5150.81
245_I248_A0.5130.81
177_S230_A0.5120.80
48_D51_K0.5120.80
114_A152_K0.5110.80
12_L67_D0.5110.80
73_A234_G0.5090.80
38_V245_I0.5080.80
244_V248_A0.5070.80
125_L164_I0.5070.80
179_T182_Q0.5060.80
177_S180_P0.5060.80
28_K32_G0.5050.80
8_G237_L0.5030.79
216_S244_V0.5030.79
214_N230_A0.5020.79
77_E101_Y0.5020.79
155_G160_E0.5010.79
67_D74_F0.5010.79
138_K145_R0.5010.79
15_S81_A0.5000.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1aw2A 2 0.9922 100 0.056 Contact Map
4mvaA 2 0.9961 100 0.061 Contact Map
3m9yA 2 0.9765 100 0.07 Contact Map
3kxqA 2 0.9333 100 0.072 Contact Map
1m6jA 2 0.9882 100 0.072 Contact Map
1o5xA 2 0.9608 100 0.072 Contact Map
3ta6A 2 0.9843 100 0.073 Contact Map
1yyaA 2 0.9765 100 0.074 Contact Map
2j27A 2 0.9686 100 0.074 Contact Map
2btmA 2 0.9765 100 0.075 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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