GREMLIN Database
RL31 - 50S ribosomal protein L31
UniProt: P0A7M9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10889
Length: 70 (65)
Sequences: 1340 (615)
Seq/√Len: 76.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
11_E23_K4.9531.00
9_Y25_R3.0261.00
14_A22_M2.7111.00
15_S21_V2.1031.00
13_T23_K2.1001.00
2_K6_H2.0551.00
20_N39_K2.0341.00
13_T21_V1.9721.00
58_D62_K1.8801.00
10_E30_H1.8351.00
18_C37_C1.7551.00
11_E25_R1.6151.00
18_C40_C1.5820.99
50_D57_V1.5370.99
13_T31_D1.4960.99
14_A34_L1.4920.99
37_C40_C1.4600.99
15_S33_N1.4130.99
14_A24_I1.4070.99
47_K54_G1.3890.99
10_E25_R1.1960.96
50_D54_G1.1610.96
58_D61_N1.1360.95
61_N64_F1.1260.95
49_R52_A1.0880.94
12_I30_H1.0640.93
17_S36_V1.0560.93
36_V51_V1.0340.92
12_I28_V1.0180.91
3_K28_V1.0150.91
19_G40_C1.0030.91
19_G37_C0.9960.91
56_R63_R0.9930.90
12_I31_D0.9890.90
4_D8_K0.9850.90
44_F51_V0.9830.90
14_A32_L0.9550.89
14_A21_V0.9040.86
61_N66_I0.9000.86
32_L35_D0.8940.85
15_S31_D0.8500.82
52_A57_V0.8370.81
49_R53_T0.8340.81
36_V55_G0.8230.80
36_V58_D0.8130.79
17_S44_F0.8060.79
55_G64_F0.8030.78
47_K50_D0.7950.78
9_Y49_R0.7770.76
17_S33_N0.7670.75
20_N37_C0.7660.75
50_D53_T0.7630.75
54_G61_N0.7560.74
26_S48_Q0.7510.74
36_V52_A0.7490.73
36_V50_D0.7380.72
12_I26_S0.7280.71
58_D66_I0.7180.70
20_N38_S0.7170.70
2_K23_K0.7100.69
8_K30_H0.7100.69
59_R62_K0.6940.68
55_G62_K0.6850.67
20_N40_C0.6820.66
14_A35_D0.6730.65
16_C37_C0.6680.65
3_K8_K0.6660.64
51_V58_D0.6620.64
53_T61_N0.6530.63
33_N44_F0.6520.63
17_S34_L0.6430.62
16_C40_C0.6370.61
35_D53_T0.6200.59
4_D21_V0.6070.57
56_R64_F0.6020.57
42_P52_A0.5890.55
18_C39_K0.5830.54
35_D52_A0.5710.53
24_I48_Q0.5650.52
54_G58_D0.5600.51
22_M39_K0.5570.51
36_V62_K0.5500.50
12_I15_S0.5460.50
5_I10_E0.5420.49
17_S57_V0.5400.49
17_S40_C0.5400.49
53_T57_V0.5360.48
4_D30_H0.5320.48
54_G63_R0.5320.48
30_H34_L0.5290.47
61_N65_N0.5200.46
36_V44_F0.5170.46
17_S37_C0.5120.45
48_Q63_R0.5060.45
8_K24_I0.5050.44
36_V54_G0.5020.44
39_K54_G0.5000.44
38_S62_K0.5000.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vs6Z 1 1 100 0.124 Contact Map
3f1f4 1 0.4286 100 0.129 Contact Map
1nkwY 1 0.9857 100 0.138 Contact Map
4rb64 1 0.9857 100 0.14 Contact Map
3bbo1 1 0.9714 100 0.185 Contact Map
1vw4V 1 0.6571 99.9 0.3 Contact Map
1x0gA 4 0.3857 28 0.908 Contact Map
2apnA 1 0.3429 27.4 0.909 Contact Map
2d2aA 2 0.3429 27.1 0.909 Contact Map
3cb4D 1 0.5857 20.7 0.913 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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