RECA - ECOLI - GREMLIN Contacts

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RECA - Protein RecA

UniProt:	P0A7G6	Sequences: MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10823
Length:	353 (329)
Sequences:	2521 (964)
Seq/√Len:	53.2

ECOLI - RECA
BACSU - RECA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
111_D	114_N	6.461	1.00
57_L	80_V	5.249	1.00
198_M	214_N	4.195	1.00
224_L	245_V	4.020	1.00
308_A	312_D	3.765	1.00
267_E	311_K	3.721	1.00
80_V	141_V	3.597	1.00
45_S	274_E	3.457	1.00
131_D	182_N	3.378	1.00
319_E	323_K	3.310	1.00
54_A	252_I	3.238	1.00
47_S	274_E	3.042	1.00
264_L	307_T	3.020	1.00
52_L	222_V	2.989	1.00
43_T	57_L	2.976	1.00
309_W	316_T	2.975	1.00
57_L	189_L	2.958	1.00
224_L	247_V	2.896	1.00
42_S	87_E	2.831	1.00
40_T	54_A	2.775	1.00
245_V	261_F	2.720	1.00
19_E	24_K	2.612	1.00
321_E	325_R	2.566	1.00
63_V	189_L	2.501	1.00
94_I	130_C	2.465	1.00
284_L	320_I	2.447	1.00
279_G	324_V	2.436	1.00
121_D	125_Q	2.398	1.00
161_G	174_Q	2.384	1.00
295_K	323_K	2.334	1.00
229_I	244_R	2.333	1.00
275_L	317_A	2.322	1.00
309_W	313_N	2.271	1.00
41_I	84_A	2.243	1.00
294_Y	319_E	2.222	1.00
84_A	141_V	2.185	1.00
272_Y	311_K	2.158	1.00
183_L	190_L	2.144	1.00
91_C	141_V	2.122	1.00
231_A	238_V	2.119	1.00
43_T	80_V	2.086	1.00
200_I	209_T	2.058	1.00
122_T	156_I	1.970	1.00
244_R	258_Q	1.948	1.00
202_V	206_N	1.931	1.00
164_H	174_Q	1.925	1.00
107_K	267_E	1.923	1.00
153_K	157_E	1.863	0.99
81_I	93_F	1.858	0.99
10_A	14_A	1.856	0.99
50_I	328_L	1.841	0.99
81_I	143_V	1.841	0.99
286_E	296_G	1.819	0.99
17_Q	21_Q	1.814	0.99
277_D	303_K	1.773	0.99
80_V	191_I	1.732	0.99
280_V	292_Y	1.709	0.99
41_I	189_L	1.666	0.99
299_I	316_T	1.662	0.99
295_K	319_E	1.661	0.99
202_V	207_P	1.658	0.99
196_I	225_D	1.642	0.98
154_A	167_L	1.638	0.98
242_E	260_E	1.637	0.98
313_N	316_T	1.622	0.98
18_I	26_S	1.621	0.98
81_I	91_C	1.604	0.98
304_A	307_T	1.556	0.98
77_T	193_I	1.552	0.98
11_L	15_L	1.529	0.97
282_E	328_L	1.522	0.97
76_L	226_I	1.519	0.97
171_M	176_M	1.480	0.97
230_G	242_E	1.476	0.97
276_V	285_I	1.473	0.97
318_K	321_E	1.472	0.97
5_E	8_Q	1.464	0.97
9_K	12_A	1.451	0.97
242_E	262_Q	1.422	0.96
273_G	277_D	1.420	0.96
314_P	317_A	1.417	0.96
6_N	9_K	1.399	0.96
234_E	304_A	1.379	0.95
228_R	231_A	1.374	0.95
273_G	303_K	1.374	0.95
78_L	100_L	1.362	0.95
168_A	211_T	1.361	0.95
276_V	303_K	1.357	0.95
318_K	322_K	1.355	0.95
165_M	200_I	1.333	0.94
322_K	326_E	1.332	0.94
282_E	324_V	1.311	0.94
279_G	317_A	1.310	0.94
90_T	139_V	1.301	0.94
271_F	321_E	1.301	0.94
57_L	63_V	1.287	0.93
228_R	241_S	1.282	0.93
314_P	318_K	1.281	0.93
306_A	309_W	1.278	0.93
308_A	311_K	1.272	0.93
229_I	242_E	1.268	0.93
323_K	326_E	1.261	0.93
232_V	239_V	1.243	0.92
92_A	133_L	1.214	0.91
79_Q	83_A	1.212	0.91
200_I	214_N	1.211	0.91
274_E	278_L	1.210	0.91
68_P	227_R	1.208	0.91
18_I	27_I	1.191	0.90
130_C	190_L	1.190	0.90
46_L	50_I	1.178	0.90
68_P	225_D	1.169	0.89
164_H	167_L	1.164	0.89
225_D	229_I	1.153	0.89
272_Y	317_A	1.151	0.89
39_E	59_M	1.149	0.89
293_S	298_K	1.146	0.88
29_R	114_N	1.139	0.88
62_I	180_A	1.131	0.88
262_Q	303_K	1.126	0.88
14_A	132_A	1.124	0.87
282_E	327_L	1.122	0.87
93_F	100_L	1.119	0.87
59_M	84_A	1.101	0.86
183_L	189_L	1.096	0.86
203_M	209_T	1.094	0.86
137_G	266_G	1.089	0.86
30_L	112_I	1.087	0.86
141_V	191_I	1.070	0.85
291_W	301_Q	1.057	0.84
244_R	260_E	1.054	0.84
184_K	187_N	1.054	0.84
9_K	13_A	1.052	0.84
165_M	203_M	1.052	0.84
201_G	207_P	1.045	0.83
300_G	306_A	1.044	0.83
57_L	141_V	1.043	0.83
303_K	307_T	1.042	0.83
47_S	278_L	1.035	0.83
283_K	327_L	1.026	0.82
211_T	214_N	1.017	0.82
13_A	17_Q	1.015	0.81
130_C	144_V	1.012	0.81
134_A	183_L	1.008	0.81
182_N	186_S	1.003	0.81
163_S	174_Q	1.003	0.81
96_A	150_L	0.994	0.80
299_I	309_W	0.989	0.80
242_E	303_K	0.986	0.80
48_L	224_L	0.985	0.79
14_A	17_Q	0.983	0.79
315_E	318_K	0.977	0.79
130_C	134_A	0.976	0.79
14_A	18_I	0.975	0.79
323_K	327_L	0.972	0.79
151_T	181_G	0.969	0.78
92_A	116_L	0.959	0.78
8_Q	90_T	0.957	0.77
139_V	142_I	0.955	0.77
326_E	330_S	0.954	0.77
33_D	36_M	0.952	0.77
42_S	49_D	0.945	0.77
8_Q	12_A	0.942	0.76
242_E	277_D	0.938	0.76
78_L	93_F	0.936	0.76
83_A	271_F	0.926	0.75
206_N	225_D	0.922	0.75
59_M	89_K	0.919	0.75
103_I	107_K	0.918	0.74
286_E	295_K	0.917	0.74
276_V	279_G	0.915	0.74
96_A	152_P	0.910	0.74
83_A	87_E	0.903	0.73
40_T	252_I	0.898	0.73
130_C	142_I	0.893	0.72
276_V	306_A	0.892	0.72
270_N	307_T	0.891	0.72
81_I	141_V	0.882	0.71
12_A	16_G	0.876	0.71
311_K	317_A	0.873	0.71
208_E	227_R	0.868	0.70
139_V	188_T	0.864	0.70
93_F	117_C	0.863	0.70
274_E	279_G	0.862	0.70
122_T	153_K	0.858	0.69
74_T	93_F	0.852	0.69
317_A	321_E	0.849	0.69
47_S	259_A	0.848	0.68
89_K	140_D	0.847	0.68
204_F	207_P	0.845	0.68
163_S	167_L	0.844	0.68
50_I	281_K	0.842	0.68
288_A	298_K	0.841	0.68
284_L	324_V	0.841	0.68
283_K	286_E	0.840	0.68
45_S	269_I	0.837	0.67
154_A	164_H	0.835	0.67
122_T	157_E	0.832	0.67
84_A	114_N	0.832	0.67
7_K	10_A	0.831	0.67
38_V	252_I	0.831	0.67
179_L	183_L	0.823	0.66
36_M	102_P	0.823	0.66
290_A	301_Q	0.823	0.66
13_A	16_G	0.820	0.66
11_L	138_A	0.818	0.66
239_V	264_L	0.811	0.65
298_K	305_N	0.810	0.65
134_A	171_M	0.810	0.65
317_A	324_V	0.806	0.65
15_L	27_I	0.806	0.65
262_Q	270_N	0.806	0.65
93_F	98_H	0.806	0.65
328_L	331_N	0.805	0.64
160_I	178_K	0.799	0.64
324_V	328_L	0.799	0.64
47_S	50_I	0.797	0.64
14_A	137_G	0.793	0.63
96_A	211_T	0.790	0.63
315_E	319_E	0.789	0.63
200_I	230_G	0.787	0.63
310_L	320_I	0.787	0.63
262_Q	273_G	0.785	0.62
64_E	223_R	0.784	0.62
271_F	275_L	0.784	0.62
196_I	286_E	0.780	0.62
5_E	12_A	0.775	0.61
270_N	304_A	0.771	0.61
46_L	325_R	0.769	0.61
241_S	263_I	0.767	0.61
10_A	24_K	0.763	0.60
147_V	172_M	0.763	0.60
30_L	93_F	0.758	0.60
77_T	81_I	0.753	0.59
135_R	186_S	0.747	0.59
76_L	190_L	0.743	0.58
9_K	16_G	0.742	0.58
238_V	265_Y	0.742	0.58
322_K	329_L	0.742	0.58
77_T	93_F	0.741	0.58
226_I	245_V	0.738	0.58
11_L	32_E	0.737	0.58
200_I	211_T	0.733	0.57
107_K	286_E	0.732	0.57
322_K	331_N	0.730	0.57
30_L	106_R	0.727	0.57
102_P	214_N	0.726	0.56
151_T	214_N	0.726	0.56
21_Q	230_G	0.723	0.56
155_E	177_R	0.723	0.56
36_M	39_E	0.723	0.56
237_N	257_K	0.720	0.56
104_Y	266_G	0.719	0.56
65_I	191_I	0.717	0.56
267_E	270_N	0.717	0.56
132_A	325_R	0.716	0.55
241_S	265_Y	0.715	0.55
48_L	52_L	0.708	0.55
73_K	145_D	0.707	0.54
56_G	73_K	0.707	0.54
79_Q	89_K	0.706	0.54
294_Y	320_I	0.704	0.54
198_M	211_T	0.702	0.54
127_L	175_A	0.701	0.54
19_E	27_I	0.691	0.53
226_I	243_T	0.690	0.53
39_E	130_C	0.690	0.53
289_G	302_G	0.689	0.53
8_Q	231_A	0.688	0.52
39_E	187_N	0.687	0.52
230_G	236_E	0.685	0.52
328_L	332_P	0.684	0.52
236_E	258_Q	0.680	0.52
5_E	318_K	0.678	0.51
278_L	282_E	0.676	0.51
8_Q	16_G	0.675	0.51
56_G	67_G	0.673	0.51
141_V	189_L	0.669	0.50
131_D	242_E	0.665	0.50
307_T	311_K	0.665	0.50
5_E	9_K	0.664	0.50
6_N	13_A	0.663	0.50
306_A	310_L	0.662	0.50
98_H	126_A	0.661	0.50
76_L	191_I	0.659	0.49
36_M	40_T	0.658	0.49
130_C	179_L	0.656	0.49
193_I	198_M	0.656	0.49
16_G	20_K	0.655	0.49
9_K	22_F	0.655	0.49
279_G	286_E	0.654	0.49
326_E	329_L	0.650	0.48
305_N	308_A	0.644	0.48
116_L	139_V	0.643	0.48
231_A	258_Q	0.642	0.48
16_G	34_R	0.639	0.47
280_V	287_K	0.639	0.47
14_A	135_R	0.637	0.47
165_M	204_F	0.635	0.47
48_L	76_L	0.634	0.47
192_F	216_L	0.633	0.47
16_G	117_C	0.633	0.47
32_E	35_S	0.633	0.47
125_Q	287_K	0.632	0.47
296_G	299_I	0.632	0.47
84_A	189_L	0.631	0.46
67_G	73_K	0.629	0.46
138_A	326_E	0.629	0.46
116_L	133_L	0.628	0.46
22_F	196_I	0.628	0.46
29_R	32_E	0.627	0.46
322_K	330_S	0.625	0.46
293_S	306_A	0.624	0.46
325_R	328_L	0.623	0.46
56_G	145_D	0.621	0.45
146_S	212_G	0.620	0.45
149_A	216_L	0.619	0.45
201_G	204_F	0.619	0.45
50_I	282_E	0.618	0.45
295_K	298_K	0.618	0.45
10_A	13_A	0.617	0.45
243_T	265_Y	0.615	0.45
193_I	214_N	0.615	0.45
277_D	280_V	0.612	0.44
19_E	26_S	0.612	0.44
231_A	243_T	0.612	0.44
193_I	298_K	0.611	0.44
90_T	180_A	0.611	0.44
39_E	87_E	0.610	0.44
264_L	273_G	0.609	0.44
285_I	306_A	0.608	0.44
79_Q	269_I	0.608	0.44
79_Q	84_A	0.607	0.44
80_V	91_C	0.607	0.44
182_N	235_G	0.607	0.44
259_A	275_L	0.607	0.44
7_K	12_A	0.606	0.44
160_I	171_M	0.605	0.44
11_L	102_P	0.604	0.44
10_A	17_Q	0.604	0.44
151_T	156_I	0.604	0.44
228_R	244_R	0.603	0.44
199_K	207_P	0.603	0.44
162_D	167_L	0.602	0.43
131_D	135_R	0.602	0.43
33_D	274_E	0.602	0.43
316_T	319_E	0.600	0.43
83_A	86_R	0.600	0.43
16_G	330_S	0.599	0.43
107_K	266_G	0.598	0.43
327_L	332_P	0.598	0.43
48_L	191_I	0.597	0.43
192_F	250_N	0.597	0.43
6_N	19_E	0.596	0.43
27_I	116_L	0.596	0.43
100_L	110_V	0.596	0.43
306_A	328_L	0.595	0.43
250_N	253_A	0.595	0.43
63_V	222_V	0.592	0.42
52_L	247_V	0.592	0.42
261_F	269_I	0.592	0.42
80_V	189_L	0.591	0.42
234_E	239_V	0.588	0.42
204_F	238_V	0.588	0.42
283_K	288_A	0.588	0.42
59_M	140_D	0.587	0.42
203_M	206_N	0.587	0.42
159_E	178_K	0.586	0.42
14_A	21_Q	0.583	0.41
327_L	330_S	0.582	0.41
327_L	331_N	0.581	0.41
84_A	91_C	0.581	0.41
90_T	114_N	0.580	0.41
196_I	230_G	0.580	0.41
323_K	329_L	0.580	0.41
104_Y	265_Y	0.580	0.41
9_K	17_Q	0.576	0.41
316_T	324_V	0.575	0.41
98_H	184_K	0.575	0.41
203_M	207_P	0.574	0.41
8_Q	13_A	0.572	0.40
97_E	146_S	0.572	0.40
170_R	173_S	0.571	0.40
312_D	332_P	0.570	0.40
234_E	237_N	0.569	0.40
20_K	236_E	0.568	0.40
16_G	286_E	0.566	0.40
34_R	37_D	0.565	0.40
225_D	244_R	0.564	0.40
326_E	331_N	0.563	0.39
242_E	304_A	0.562	0.39
134_A	179_L	0.562	0.39
188_T	272_Y	0.561	0.39
66_Y	225_D	0.560	0.39
196_I	214_N	0.560	0.39
91_C	131_D	0.559	0.39
272_Y	307_T	0.557	0.39
15_L	116_L	0.556	0.39
316_T	320_I	0.556	0.39
27_I	92_A	0.555	0.39
233_K	236_E	0.554	0.39
282_E	330_S	0.554	0.39
176_M	182_N	0.554	0.39
238_V	243_T	0.553	0.38
15_L	132_A	0.552	0.38
18_I	23_G	0.552	0.38
12_A	46_L	0.552	0.38
14_A	189_L	0.552	0.38
184_K	242_E	0.552	0.38
229_I	258_Q	0.551	0.38
318_K	326_E	0.551	0.38
89_K	185_Q	0.549	0.38
92_A	219_Y	0.549	0.38
322_K	332_P	0.548	0.38
276_V	297_E	0.548	0.38
144_V	176_M	0.548	0.38
112_I	234_E	0.547	0.38
206_N	227_R	0.547	0.38
121_D	160_I	0.547	0.38
50_I	330_S	0.547	0.38
16_G	196_I	0.545	0.38
7_K	327_L	0.545	0.38
32_E	329_L	0.544	0.38
180_A	211_T	0.543	0.37
318_K	331_N	0.543	0.37
320_I	324_V	0.542	0.37
35_S	102_P	0.541	0.37
204_F	239_V	0.541	0.37
112_I	193_I	0.538	0.37
236_E	242_E	0.538	0.37
46_L	324_V	0.538	0.37
108_L	132_A	0.537	0.37
176_M	192_F	0.536	0.37
13_A	116_L	0.535	0.37
322_K	327_L	0.534	0.37
29_R	154_A	0.534	0.37
184_K	243_T	0.533	0.36
207_P	227_R	0.532	0.36
262_Q	307_T	0.531	0.36
54_A	132_A	0.530	0.36
302_G	305_N	0.530	0.36
159_E	164_H	0.529	0.36
321_E	324_V	0.529	0.36
21_Q	24_K	0.527	0.36
134_A	142_I	0.526	0.36
47_S	57_L	0.525	0.36
67_G	145_D	0.525	0.36
16_G	283_K	0.525	0.36
116_L	132_A	0.525	0.36
189_L	325_R	0.525	0.36
286_E	293_S	0.525	0.36
222_V	252_I	0.524	0.36
21_Q	76_L	0.524	0.36
270_N	273_G	0.523	0.35
97_E	212_G	0.523	0.35
13_A	331_N	0.522	0.35
16_G	333_N	0.522	0.35
242_E	273_G	0.522	0.35
306_A	313_N	0.520	0.35
6_N	329_L	0.520	0.35
43_T	49_D	0.520	0.35
37_D	226_I	0.519	0.35
8_Q	33_D	0.518	0.35
38_V	221_S	0.517	0.35
117_C	261_F	0.517	0.35
267_E	272_Y	0.516	0.35
8_Q	132_A	0.516	0.35
282_E	329_L	0.516	0.35
264_L	270_N	0.516	0.35
62_I	187_N	0.516	0.35
45_S	230_G	0.516	0.35
275_L	321_E	0.515	0.35
234_E	308_A	0.515	0.35
47_S	261_F	0.515	0.35
310_L	316_T	0.515	0.35
12_A	330_S	0.514	0.35
243_T	261_F	0.513	0.34
322_K	333_N	0.510	0.34
108_L	291_W	0.510	0.34
29_R	313_N	0.510	0.34
57_L	81_I	0.510	0.34
32_E	36_M	0.510	0.34
243_T	257_K	0.509	0.34
59_M	87_E	0.507	0.34
16_G	329_L	0.506	0.34
43_T	222_V	0.506	0.34
137_G	186_S	0.504	0.34
18_I	22_F	0.503	0.34
6_N	309_W	0.503	0.34
6_N	17_Q	0.502	0.33
231_A	265_Y	0.502	0.33
234_E	283_K	0.502	0.33
10_A	19_E	0.500	0.33
159_E	204_F	0.500	0.33

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3hr8A	1	0.9518	100	0.363	Contact Map
4ppfA	1	0.8782	100	0.365	Contact Map
3cmwA	1	0.8924	100	0.37	Contact Map
3cmuA	1	0.8867	100	0.375	Contact Map
1u94A	3	0.8669	100	0.387	Contact Map
1xp8A	3	0.8414	100	0.434	Contact Map
3io5A	2	0.7082	100	0.438	Contact Map
2z43A	1	0.6572	100	0.637	Contact Map
3ldaA	1	0.6544	100	0.641	Contact Map
1v5wA	5	0.6232	100	0.643	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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