PFKA - ECOLI - GREMLIN Contacts

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PFKA - ATP-dependent 6-phosphofructokinase isozyme 1

UniProt:	P0A796	Sequences: MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLGLYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIGGDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSSHQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAIVAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLLLAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10699
Length:	320 (305)
Sequences:	4629 (2523)
Seq/√Len:	144.5

ECOLI - PFKA
BACSU - PFKA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
4_K	34_E	4.571	1.00
34_E	52_Q	3.705	1.00
182_A	190_V	3.410	1.00
90_L	95_I	3.239	1.00
6_G	38_I	3.233	1.00
39_Y	51_V	3.147	1.00
100_V	106_S	3.062	1.00
283_R	294_H	2.930	1.00
88_E	92_K	2.861	1.00
111_M	115_E	2.778	1.00
141_F	258_V	2.645	1.00
48_D	92_K	2.560	1.00
54_D	57_S	2.525	1.00
178_T	220_A	2.504	1.00
37_G	69_L	2.439	1.00
275_L	294_H	2.427	1.00
147_V	177_L	2.355	1.00
48_D	93_R	2.347	1.00
280_Y	283_R	2.296	1.00
200_E	238_E	2.259	1.00
39_Y	49_R	2.192	1.00
234_F	238_E	2.159	1.00
151_I	220_A	2.144	1.00
43_L	47_E	2.144	1.00
48_D	89_N	2.138	1.00
233_H	237_K	2.136	1.00
36_M	52_Q	2.122	1.00
50_M	93_R	2.110	1.00
89_N	92_K	2.100	1.00
141_F	145_S	2.069	1.00
36_M	50_M	2.051	1.00
144_L	177_L	2.016	1.00
28_A	35_V	2.002	1.00
33_L	277_L	1.979	1.00
4_K	36_M	1.968	1.00
37_G	53_L	1.947	1.00
207_K	211_A	1.938	1.00
7_V	24_V	1.936	1.00
235_I	239_T	1.929	1.00
192_V	307_P	1.901	1.00
6_G	95_I	1.893	1.00
24_V	99_V	1.886	1.00
145_S	258_V	1.869	1.00
27_S	274_D	1.836	1.00
79_D	82_I	1.831	1.00
271_Y	274_D	1.824	1.00
286_G	293_V	1.803	1.00
40_D	49_R	1.802	1.00
271_Y	292_L	1.778	1.00
234_F	237_K	1.773	1.00
169_V	220_A	1.762	1.00
140_F	180_A	1.739	1.00
84_A	88_E	1.724	1.00
147_V	181_A	1.717	1.00
274_D	278_A	1.690	1.00
203_V	238_E	1.682	1.00
283_R	296_D	1.677	1.00
280_Y	294_H	1.669	1.00
115_E	298_I	1.667	1.00
288_Q	293_V	1.666	1.00
4_K	94_G	1.649	1.00
61_M	68_F	1.633	1.00
150_A	251_I	1.620	1.00
38_I	50_M	1.604	1.00
99_V	276_L	1.576	1.00
80_E	84_A	1.563	1.00
189_F	202_L	1.559	1.00
29_L	55_R	1.559	1.00
208_A	211_A	1.552	1.00
38_I	45_L	1.543	1.00
20_A	123_L	1.542	1.00
40_D	162_Q	1.539	1.00
154_L	167_V	1.532	1.00
51_V	57_S	1.517	1.00
149_E	153_R	1.514	1.00
166_V	231_L	1.512	1.00
31_E	277_L	1.492	1.00
107_Y	122_G	1.486	1.00
88_E	91_K	1.476	1.00
40_D	70_G	1.471	1.00
203_V	235_I	1.465	1.00
112_R	115_E	1.420	1.00
229_D	233_H	1.407	1.00
87_I	116_M	1.399	1.00
75_P	78_R	1.393	1.00
151_I	167_V	1.392	1.00
151_I	185_G	1.376	1.00
42_Y	109_G	1.356	1.00
97_A	276_L	1.342	1.00
199_R	234_F	1.336	1.00
85_V	88_E	1.319	1.00
138_I	265_A	1.308	1.00
206_I	217_A	1.270	1.00
22_R	266_S	1.265	1.00
288_Q	295_H	1.260	1.00
169_V	178_T	1.255	1.00
151_I	218_I	1.254	1.00
24_V	273_I	1.254	1.00
152_D	262_R	1.248	1.00
8_L	45_L	1.246	1.00
275_L	283_R	1.244	1.00
3_K	96_D	1.244	1.00
286_G	295_H	1.244	1.00
221_I	231_L	1.241	1.00
114_T	282_G	1.235	1.00
271_Y	275_L	1.231	1.00
204_N	208_A	1.221	1.00
24_V	269_G	1.215	1.00
296_D	299_D	1.213	1.00
166_V	219_V	1.208	1.00
111_M	298_I	1.204	1.00
27_S	31_E	1.191	1.00
28_A	33_L	1.183	1.00
230_E	234_F	1.172	1.00
232_A	245_A	1.165	1.00
7_V	101_I	1.164	1.00
36_M	93_R	1.162	1.00
87_I	91_K	1.159	1.00
121_I	283_R	1.157	1.00
10_S	106_S	1.155	1.00
271_Y	294_H	1.152	1.00
20_A	138_I	1.150	0.99
25_V	58_V	1.136	0.99
188_E	214_K	1.128	0.99
46_Y	74_F	1.118	0.99
110_A	120_C	1.105	0.99
73_R	76_E	1.101	0.99
246_T	251_I	1.097	0.99
90_L	98_L	1.091	0.99
151_I	181_A	1.085	0.99
5_I	99_V	1.072	0.99
100_V	110_A	1.069	0.99
284_C	295_H	1.066	0.99
9_T	17_M	1.066	0.99
8_L	38_I	1.065	0.99
105_G	109_G	1.063	0.99
75_P	105_G	1.062	0.99
201_D	204_N	1.057	0.99
43_L	74_F	1.057	0.99
27_S	270_A	1.057	0.99
143_A	177_L	1.052	0.99
70_G	162_Q	1.050	0.99
236_E	242_E	1.050	0.99
175_G	194_E	1.049	0.99
82_I	85_V	1.048	0.99
84_A	116_M	1.048	0.99
107_Y	284_C	1.047	0.99
132_K	303_N	1.040	0.99
146_T	153_R	1.037	0.99
275_L	285_V	1.035	0.99
5_I	28_A	1.033	0.99
36_M	95_I	1.030	0.99
188_E	209_G	1.021	0.99
28_A	273_I	1.007	0.99
209_G	212_K	1.007	0.99
207_K	238_E	1.004	0.99
205_E	208_A	1.003	0.99
83_R	112_R	1.002	0.99
76_E	85_V	1.002	0.99
25_V	29_L	0.992	0.99
114_T	281_G	0.985	0.98
108_M	112_R	0.983	0.98
23_G	269_G	0.979	0.98
246_T	249_G	0.978	0.98
173_Y	304_M	0.975	0.98
208_A	212_K	0.970	0.98
233_H	236_E	0.970	0.98
110_A	297_I	0.961	0.98
25_V	35_V	0.954	0.98
8_L	100_V	0.945	0.98
12_G	128_D	0.943	0.98
179_L	192_V	0.939	0.98
144_L	147_V	0.939	0.98
198_S	201_D	0.928	0.98
158_S	165_S	0.926	0.98
166_V	243_T	0.926	0.98
136_Y	264_L	0.912	0.97
6_G	98_L	0.911	0.97
146_T	149_E	0.905	0.97
46_Y	89_N	0.891	0.97
17_M	125_G	0.890	0.97
204_N	207_K	0.890	0.97
231_L	235_I	0.888	0.97
127_I	170_M	0.882	0.97
167_V	218_I	0.879	0.97
232_A	236_E	0.878	0.97
164_I	235_I	0.873	0.97
101_I	125_G	0.865	0.96
154_L	165_S	0.865	0.96
9_T	67_T	0.864	0.96
295_H	300_A	0.863	0.96
7_V	21_I	0.856	0.96
73_R	77_F	0.853	0.96
259_P	263_I	0.847	0.96
148_V	185_G	0.846	0.96
232_A	243_T	0.845	0.96
127_I	177_L	0.843	0.96
87_I	118_F	0.838	0.96
136_Y	260_Y	0.837	0.96
260_Y	264_L	0.833	0.96
120_C	297_I	0.832	0.96
12_G	73_R	0.832	0.96
165_S	246_T	0.830	0.96
13_D	157_T	0.822	0.95
229_D	232_A	0.818	0.95
209_G	217_A	0.815	0.95
128_D	172_R	0.814	0.95
84_A	91_K	0.813	0.95
74_F	82_I	0.807	0.95
83_R	116_M	0.807	0.95
6_G	36_M	0.806	0.95
210_I	241_R	0.805	0.95
74_F	105_G	0.795	0.94
104_D	108_M	0.794	0.94
132_K	300_A	0.789	0.94
267_R	292_L	0.788	0.94
5_I	273_I	0.788	0.94
85_V	89_N	0.786	0.94
133_G	300_A	0.785	0.94
203_V	207_K	0.783	0.94
229_D	247_V	0.780	0.94
70_G	215_K	0.778	0.94
229_D	245_A	0.773	0.93
114_T	120_C	0.772	0.93
14_A	17_M	0.770	0.93
189_F	205_E	0.769	0.93
87_I	113_L	0.768	0.93
264_L	267_R	0.765	0.93
26_R	266_S	0.764	0.93
166_V	232_A	0.764	0.93
297_I	301_I	0.758	0.93
182_A	187_C	0.756	0.93
97_A	119_P	0.756	0.93
228_V	235_I	0.755	0.93
200_E	204_N	0.753	0.93
89_N	93_R	0.752	0.93
108_M	301_I	0.752	0.93
268_M	287_I	0.750	0.92
21_I	68_F	0.750	0.92
201_D	205_E	0.750	0.92
239_T	243_T	0.749	0.92
166_V	235_I	0.746	0.92
230_E	233_H	0.745	0.92
144_L	259_P	0.744	0.92
31_E	274_D	0.743	0.92
21_I	101_I	0.743	0.92
20_A	101_I	0.741	0.92
25_V	53_L	0.741	0.92
147_V	248_L	0.739	0.92
268_M	292_L	0.738	0.92
90_L	118_F	0.733	0.92
123_L	285_V	0.732	0.91
285_V	294_H	0.732	0.91
105_G	128_D	0.732	0.91
228_V	245_A	0.730	0.91
67_T	160_S	0.730	0.91
25_V	28_A	0.728	0.91
9_T	13_D	0.724	0.91
73_R	78_R	0.722	0.91
175_G	193_P	0.721	0.91
5_I	97_A	0.721	0.91
104_D	174_C	0.716	0.91
275_L	280_Y	0.715	0.91
141_F	260_Y	0.713	0.90
263_I	267_R	0.713	0.90
4_K	95_I	0.713	0.90
131_I	300_A	0.707	0.90
293_V	296_D	0.707	0.90
232_A	239_T	0.706	0.90
133_G	295_H	0.704	0.90
108_M	111_M	0.703	0.90
204_N	211_A	0.700	0.90
38_I	89_N	0.699	0.90
77_F	83_R	0.698	0.89
231_L	239_T	0.693	0.89
140_F	176_D	0.693	0.89
268_M	285_V	0.692	0.89
43_L	76_E	0.690	0.89
119_P	281_G	0.690	0.89
131_I	268_M	0.687	0.89
123_L	138_I	0.685	0.89
169_V	177_L	0.685	0.89
174_C	180_A	0.685	0.89
196_E	226_C	0.683	0.88
12_G	104_D	0.679	0.88
287_I	290_E	0.679	0.88
134_T	284_C	0.676	0.88
189_F	219_V	0.674	0.88
244_R	250_H	0.672	0.88
130_D	253_R	0.671	0.88
248_L	252_Q	0.670	0.87
4_K	96_D	0.669	0.87
27_S	273_I	0.667	0.87
77_F	82_I	0.665	0.87
73_R	128_D	0.664	0.87
275_L	278_A	0.661	0.87
15_P	146_T	0.661	0.87
38_I	48_D	0.659	0.87
123_L	272_A	0.659	0.87
199_R	203_V	0.656	0.86
3_K	33_L	0.652	0.86
81_N	84_A	0.652	0.86
178_T	190_V	0.645	0.85
159_S	215_K	0.644	0.85
67_T	71_S	0.644	0.85
192_V	224_H	0.644	0.85
59_S	63_N	0.643	0.85
27_S	30_T	0.643	0.85
42_Y	45_L	0.641	0.85
170_M	252_Q	0.640	0.85
193_P	224_H	0.640	0.85
133_G	286_G	0.639	0.85
10_S	76_E	0.639	0.85
207_K	239_T	0.638	0.85
199_R	231_L	0.638	0.85
164_I	219_V	0.636	0.85
247_V	252_Q	0.636	0.85
22_R	26_R	0.636	0.85
50_M	95_I	0.635	0.85
91_K	94_G	0.633	0.84
7_V	28_A	0.633	0.84
274_D	277_L	0.632	0.84
45_L	98_L	0.630	0.84
33_L	276_L	0.630	0.84
235_I	243_T	0.629	0.84
91_K	117_G	0.629	0.84
240_G	243_T	0.629	0.84
206_I	219_V	0.628	0.84
278_A	294_H	0.627	0.84
144_L	180_A	0.627	0.84
5_I	276_L	0.626	0.84
6_G	50_M	0.626	0.84
53_L	68_F	0.626	0.84
67_T	156_D	0.626	0.84
133_G	303_N	0.625	0.84
200_E	203_V	0.624	0.84
202_L	206_I	0.623	0.83
38_I	95_I	0.621	0.83
125_G	138_I	0.620	0.83
271_Y	278_A	0.618	0.83
104_D	172_R	0.616	0.83
90_L	119_P	0.615	0.83
154_L	157_T	0.615	0.83
173_Y	226_C	0.615	0.83
29_L	58_V	0.614	0.83
191_V	202_L	0.614	0.83
164_I	217_A	0.609	0.82
226_C	231_L	0.608	0.82
74_F	78_R	0.607	0.82
288_Q	291_Q	0.607	0.82
128_D	170_M	0.607	0.82
164_I	216_H	0.607	0.82
73_R	109_G	0.606	0.82
10_S	109_G	0.603	0.81
207_K	210_I	0.602	0.81
12_G	223_E	0.602	0.81
267_R	287_I	0.602	0.81
12_G	105_G	0.601	0.81
121_I	275_L	0.599	0.81
14_A	125_G	0.599	0.81
260_Y	287_I	0.599	0.81
57_S	60_D	0.598	0.81
155_R	216_H	0.598	0.81
176_D	308_F	0.596	0.81
58_V	61_M	0.595	0.81
26_R	59_S	0.595	0.81
74_F	77_F	0.595	0.81
260_Y	267_R	0.594	0.80
80_E	116_M	0.592	0.80
286_G	294_H	0.591	0.80
10_S	75_P	0.590	0.80
167_V	220_A	0.587	0.80
152_D	158_S	0.585	0.79
99_V	123_L	0.579	0.79
205_E	209_G	0.578	0.79
83_R	109_G	0.578	0.79
147_V	169_V	0.578	0.79
191_V	205_E	0.573	0.78
33_L	273_I	0.573	0.78
154_L	218_I	0.573	0.78
184_A	263_I	0.572	0.78
19_A	266_S	0.571	0.78
42_Y	74_F	0.570	0.78
154_L	158_S	0.569	0.78
131_I	172_R	0.568	0.77
26_R	57_S	0.567	0.77
130_D	172_R	0.565	0.77
62_I	266_S	0.565	0.77
176_D	180_A	0.564	0.77
172_R	224_H	0.564	0.77
147_V	151_I	0.564	0.77
38_I	93_R	0.563	0.77
75_P	79_D	0.561	0.77
42_Y	105_G	0.559	0.76
295_H	299_D	0.559	0.76
8_L	98_L	0.557	0.76
111_M	297_I	0.556	0.76
53_L	69_L	0.556	0.76
197_F	225_M	0.552	0.75
75_P	86_A	0.551	0.75
83_R	87_I	0.550	0.75
192_V	195_V	0.549	0.75
53_L	58_V	0.548	0.75
104_D	128_D	0.548	0.75
135_D	289_N	0.546	0.75
221_I	228_V	0.545	0.74
64_R	256_S	0.545	0.74
30_T	277_L	0.544	0.74
203_V	234_F	0.544	0.74
10_S	13_D	0.543	0.74
268_M	286_G	0.543	0.74
155_R	218_I	0.541	0.74
111_M	282_G	0.541	0.74
69_L	101_I	0.540	0.74
16_G	20_A	0.539	0.74
42_Y	137_T	0.539	0.74
167_V	185_G	0.538	0.74
232_A	235_I	0.537	0.73
276_L	279_G	0.537	0.73
104_D	130_D	0.535	0.73
29_L	35_V	0.531	0.73
284_C	297_I	0.529	0.72
6_G	90_L	0.529	0.72
154_L	246_T	0.528	0.72
196_E	225_M	0.526	0.72
78_R	83_R	0.525	0.72
86_A	113_L	0.524	0.72
26_R	55_R	0.523	0.71
45_L	86_A	0.521	0.71
12_G	130_D	0.519	0.71
212_K	219_V	0.518	0.71
155_R	159_S	0.518	0.71
155_R	209_G	0.517	0.71
48_D	85_V	0.517	0.71
15_P	142_T	0.517	0.71
220_A	231_L	0.517	0.71
111_M	114_T	0.516	0.70
19_A	62_I	0.516	0.70
144_L	181_A	0.515	0.70
270_A	274_D	0.515	0.70
7_V	69_L	0.513	0.70
60_D	216_H	0.512	0.70
209_G	216_H	0.512	0.70
130_D	170_M	0.512	0.70
59_S	209_G	0.510	0.70
26_R	30_T	0.510	0.70
11_G	103_G	0.509	0.69
23_G	138_I	0.509	0.69
189_F	217_A	0.507	0.69
134_T	286_G	0.506	0.69
73_R	105_G	0.506	0.69
105_G	108_M	0.505	0.69
72_A	106_S	0.505	0.69
134_T	268_M	0.502	0.68
287_I	292_L	0.501	0.68
189_F	201_D	0.501	0.68
24_V	101_I	0.501	0.68
111_M	301_I	0.500	0.68

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1pfkA	3	0.9969	100	0.032	Contact Map
1zxxA	3	0.9938	100	0.033	Contact Map
4i7eA	3	0.9938	100	0.034	Contact Map
3o8oB	1	1	100	0.056	Contact Map
4xz2A	3	0.9812	100	0.063	Contact Map
3hnoA	2	0.9875	100	0.064	Contact Map
3opyB	2	0.9969	100	0.067	Contact Map
3o8oA	1	0.9969	100	0.067	Contact Map
3opyA	2	0.9969	100	0.072	Contact Map
2higA	3	0.975	100	0.104	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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