GREMLIN Database
PFKA - ATP-dependent 6-phosphofructokinase isozyme 1
UniProt: P0A796 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10699
Length: 320 (305)
Sequences: 4629 (2523)
Seq/√Len: 144.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_K34_E4.5711.00
34_E52_Q3.7051.00
182_A190_V3.4101.00
90_L95_I3.2391.00
6_G38_I3.2331.00
39_Y51_V3.1471.00
100_V106_S3.0621.00
283_R294_H2.9301.00
88_E92_K2.8611.00
111_M115_E2.7781.00
141_F258_V2.6451.00
48_D92_K2.5601.00
54_D57_S2.5251.00
178_T220_A2.5041.00
37_G69_L2.4391.00
275_L294_H2.4271.00
147_V177_L2.3551.00
48_D93_R2.3471.00
280_Y283_R2.2961.00
200_E238_E2.2591.00
39_Y49_R2.1921.00
234_F238_E2.1591.00
151_I220_A2.1441.00
43_L47_E2.1441.00
48_D89_N2.1381.00
233_H237_K2.1361.00
36_M52_Q2.1221.00
50_M93_R2.1101.00
89_N92_K2.1001.00
141_F145_S2.0691.00
36_M50_M2.0511.00
144_L177_L2.0161.00
28_A35_V2.0021.00
33_L277_L1.9791.00
4_K36_M1.9681.00
37_G53_L1.9471.00
207_K211_A1.9381.00
7_V24_V1.9361.00
235_I239_T1.9291.00
192_V307_P1.9011.00
6_G95_I1.8931.00
24_V99_V1.8861.00
145_S258_V1.8691.00
27_S274_D1.8361.00
79_D82_I1.8311.00
271_Y274_D1.8241.00
286_G293_V1.8031.00
40_D49_R1.8021.00
271_Y292_L1.7781.00
234_F237_K1.7731.00
169_V220_A1.7621.00
140_F180_A1.7391.00
84_A88_E1.7241.00
147_V181_A1.7171.00
274_D278_A1.6901.00
203_V238_E1.6821.00
283_R296_D1.6771.00
280_Y294_H1.6691.00
115_E298_I1.6671.00
288_Q293_V1.6661.00
4_K94_G1.6491.00
61_M68_F1.6331.00
150_A251_I1.6201.00
38_I50_M1.6041.00
99_V276_L1.5761.00
80_E84_A1.5631.00
189_F202_L1.5591.00
29_L55_R1.5591.00
208_A211_A1.5521.00
38_I45_L1.5431.00
20_A123_L1.5421.00
40_D162_Q1.5391.00
154_L167_V1.5321.00
51_V57_S1.5171.00
149_E153_R1.5141.00
166_V231_L1.5121.00
31_E277_L1.4921.00
107_Y122_G1.4861.00
88_E91_K1.4761.00
40_D70_G1.4711.00
203_V235_I1.4651.00
112_R115_E1.4201.00
229_D233_H1.4071.00
87_I116_M1.3991.00
75_P78_R1.3931.00
151_I167_V1.3921.00
151_I185_G1.3761.00
42_Y109_G1.3561.00
97_A276_L1.3421.00
199_R234_F1.3361.00
85_V88_E1.3191.00
138_I265_A1.3081.00
206_I217_A1.2701.00
22_R266_S1.2651.00
288_Q295_H1.2601.00
169_V178_T1.2551.00
151_I218_I1.2541.00
24_V273_I1.2541.00
152_D262_R1.2481.00
8_L45_L1.2461.00
275_L283_R1.2441.00
3_K96_D1.2441.00
286_G295_H1.2441.00
221_I231_L1.2411.00
114_T282_G1.2351.00
271_Y275_L1.2311.00
204_N208_A1.2211.00
24_V269_G1.2151.00
296_D299_D1.2131.00
166_V219_V1.2081.00
111_M298_I1.2041.00
27_S31_E1.1911.00
28_A33_L1.1831.00
230_E234_F1.1721.00
232_A245_A1.1651.00
7_V101_I1.1641.00
36_M93_R1.1621.00
87_I91_K1.1591.00
121_I283_R1.1571.00
10_S106_S1.1551.00
271_Y294_H1.1521.00
20_A138_I1.1500.99
25_V58_V1.1360.99
188_E214_K1.1280.99
46_Y74_F1.1180.99
110_A120_C1.1050.99
73_R76_E1.1010.99
246_T251_I1.0970.99
90_L98_L1.0910.99
151_I181_A1.0850.99
5_I99_V1.0720.99
100_V110_A1.0690.99
284_C295_H1.0660.99
9_T17_M1.0660.99
8_L38_I1.0650.99
105_G109_G1.0630.99
75_P105_G1.0620.99
201_D204_N1.0570.99
43_L74_F1.0570.99
27_S270_A1.0570.99
143_A177_L1.0520.99
70_G162_Q1.0500.99
236_E242_E1.0500.99
175_G194_E1.0490.99
82_I85_V1.0480.99
84_A116_M1.0480.99
107_Y284_C1.0470.99
132_K303_N1.0400.99
146_T153_R1.0370.99
275_L285_V1.0350.99
5_I28_A1.0330.99
36_M95_I1.0300.99
188_E209_G1.0210.99
28_A273_I1.0070.99
209_G212_K1.0070.99
207_K238_E1.0040.99
205_E208_A1.0030.99
83_R112_R1.0020.99
76_E85_V1.0020.99
25_V29_L0.9920.99
114_T281_G0.9850.98
108_M112_R0.9830.98
23_G269_G0.9790.98
246_T249_G0.9780.98
173_Y304_M0.9750.98
208_A212_K0.9700.98
233_H236_E0.9700.98
110_A297_I0.9610.98
25_V35_V0.9540.98
8_L100_V0.9450.98
12_G128_D0.9430.98
179_L192_V0.9390.98
144_L147_V0.9390.98
198_S201_D0.9280.98
158_S165_S0.9260.98
166_V243_T0.9260.98
136_Y264_L0.9120.97
6_G98_L0.9110.97
146_T149_E0.9050.97
46_Y89_N0.8910.97
17_M125_G0.8900.97
204_N207_K0.8900.97
231_L235_I0.8880.97
127_I170_M0.8820.97
167_V218_I0.8790.97
232_A236_E0.8780.97
164_I235_I0.8730.97
101_I125_G0.8650.96
154_L165_S0.8650.96
9_T67_T0.8640.96
295_H300_A0.8630.96
7_V21_I0.8560.96
73_R77_F0.8530.96
259_P263_I0.8470.96
148_V185_G0.8460.96
232_A243_T0.8450.96
127_I177_L0.8430.96
87_I118_F0.8380.96
136_Y260_Y0.8370.96
260_Y264_L0.8330.96
120_C297_I0.8320.96
12_G73_R0.8320.96
165_S246_T0.8300.96
13_D157_T0.8220.95
229_D232_A0.8180.95
209_G217_A0.8150.95
128_D172_R0.8140.95
84_A91_K0.8130.95
74_F82_I0.8070.95
83_R116_M0.8070.95
6_G36_M0.8060.95
210_I241_R0.8050.95
74_F105_G0.7950.94
104_D108_M0.7940.94
132_K300_A0.7890.94
267_R292_L0.7880.94
5_I273_I0.7880.94
85_V89_N0.7860.94
133_G300_A0.7850.94
203_V207_K0.7830.94
229_D247_V0.7800.94
70_G215_K0.7780.94
229_D245_A0.7730.93
114_T120_C0.7720.93
14_A17_M0.7700.93
189_F205_E0.7690.93
87_I113_L0.7680.93
264_L267_R0.7650.93
26_R266_S0.7640.93
166_V232_A0.7640.93
297_I301_I0.7580.93
182_A187_C0.7560.93
97_A119_P0.7560.93
228_V235_I0.7550.93
200_E204_N0.7530.93
89_N93_R0.7520.93
108_M301_I0.7520.93
268_M287_I0.7500.92
21_I68_F0.7500.92
201_D205_E0.7500.92
239_T243_T0.7490.92
166_V235_I0.7460.92
230_E233_H0.7450.92
144_L259_P0.7440.92
31_E274_D0.7430.92
21_I101_I0.7430.92
20_A101_I0.7410.92
25_V53_L0.7410.92
147_V248_L0.7390.92
268_M292_L0.7380.92
90_L118_F0.7330.92
123_L285_V0.7320.91
285_V294_H0.7320.91
105_G128_D0.7320.91
228_V245_A0.7300.91
67_T160_S0.7300.91
25_V28_A0.7280.91
9_T13_D0.7240.91
73_R78_R0.7220.91
175_G193_P0.7210.91
5_I97_A0.7210.91
104_D174_C0.7160.91
275_L280_Y0.7150.91
141_F260_Y0.7130.90
263_I267_R0.7130.90
4_K95_I0.7130.90
131_I300_A0.7070.90
293_V296_D0.7070.90
232_A239_T0.7060.90
133_G295_H0.7040.90
108_M111_M0.7030.90
204_N211_A0.7000.90
38_I89_N0.6990.90
77_F83_R0.6980.89
231_L239_T0.6930.89
140_F176_D0.6930.89
268_M285_V0.6920.89
43_L76_E0.6900.89
119_P281_G0.6900.89
131_I268_M0.6870.89
123_L138_I0.6850.89
169_V177_L0.6850.89
174_C180_A0.6850.89
196_E226_C0.6830.88
12_G104_D0.6790.88
287_I290_E0.6790.88
134_T284_C0.6760.88
189_F219_V0.6740.88
244_R250_H0.6720.88
130_D253_R0.6710.88
248_L252_Q0.6700.87
4_K96_D0.6690.87
27_S273_I0.6670.87
77_F82_I0.6650.87
73_R128_D0.6640.87
275_L278_A0.6610.87
15_P146_T0.6610.87
38_I48_D0.6590.87
123_L272_A0.6590.87
199_R203_V0.6560.86
3_K33_L0.6520.86
81_N84_A0.6520.86
178_T190_V0.6450.85
159_S215_K0.6440.85
67_T71_S0.6440.85
192_V224_H0.6440.85
59_S63_N0.6430.85
27_S30_T0.6430.85
42_Y45_L0.6410.85
170_M252_Q0.6400.85
193_P224_H0.6400.85
133_G286_G0.6390.85
10_S76_E0.6390.85
207_K239_T0.6380.85
199_R231_L0.6380.85
164_I219_V0.6360.85
247_V252_Q0.6360.85
22_R26_R0.6360.85
50_M95_I0.6350.85
91_K94_G0.6330.84
7_V28_A0.6330.84
274_D277_L0.6320.84
45_L98_L0.6300.84
33_L276_L0.6300.84
235_I243_T0.6290.84
91_K117_G0.6290.84
240_G243_T0.6290.84
206_I219_V0.6280.84
278_A294_H0.6270.84
144_L180_A0.6270.84
5_I276_L0.6260.84
6_G50_M0.6260.84
53_L68_F0.6260.84
67_T156_D0.6260.84
133_G303_N0.6250.84
200_E203_V0.6240.84
202_L206_I0.6230.83
38_I95_I0.6210.83
125_G138_I0.6200.83
271_Y278_A0.6180.83
104_D172_R0.6160.83
90_L119_P0.6150.83
154_L157_T0.6150.83
173_Y226_C0.6150.83
29_L58_V0.6140.83
191_V202_L0.6140.83
164_I217_A0.6090.82
226_C231_L0.6080.82
74_F78_R0.6070.82
288_Q291_Q0.6070.82
128_D170_M0.6070.82
164_I216_H0.6070.82
73_R109_G0.6060.82
10_S109_G0.6030.81
207_K210_I0.6020.81
12_G223_E0.6020.81
267_R287_I0.6020.81
12_G105_G0.6010.81
121_I275_L0.5990.81
14_A125_G0.5990.81
260_Y287_I0.5990.81
57_S60_D0.5980.81
155_R216_H0.5980.81
176_D308_F0.5960.81
58_V61_M0.5950.81
26_R59_S0.5950.81
74_F77_F0.5950.81
260_Y267_R0.5940.80
80_E116_M0.5920.80
286_G294_H0.5910.80
10_S75_P0.5900.80
167_V220_A0.5870.80
152_D158_S0.5850.79
99_V123_L0.5790.79
205_E209_G0.5780.79
83_R109_G0.5780.79
147_V169_V0.5780.79
191_V205_E0.5730.78
33_L273_I0.5730.78
154_L218_I0.5730.78
184_A263_I0.5720.78
19_A266_S0.5710.78
42_Y74_F0.5700.78
154_L158_S0.5690.78
131_I172_R0.5680.77
26_R57_S0.5670.77
130_D172_R0.5650.77
62_I266_S0.5650.77
176_D180_A0.5640.77
172_R224_H0.5640.77
147_V151_I0.5640.77
38_I93_R0.5630.77
75_P79_D0.5610.77
42_Y105_G0.5590.76
295_H299_D0.5590.76
8_L98_L0.5570.76
111_M297_I0.5560.76
53_L69_L0.5560.76
197_F225_M0.5520.75
75_P86_A0.5510.75
83_R87_I0.5500.75
192_V195_V0.5490.75
53_L58_V0.5480.75
104_D128_D0.5480.75
135_D289_N0.5460.75
221_I228_V0.5450.74
64_R256_S0.5450.74
30_T277_L0.5440.74
203_V234_F0.5440.74
10_S13_D0.5430.74
268_M286_G0.5430.74
155_R218_I0.5410.74
111_M282_G0.5410.74
69_L101_I0.5400.74
16_G20_A0.5390.74
42_Y137_T0.5390.74
167_V185_G0.5380.74
232_A235_I0.5370.73
276_L279_G0.5370.73
104_D130_D0.5350.73
29_L35_V0.5310.73
284_C297_I0.5290.72
6_G90_L0.5290.72
154_L246_T0.5280.72
196_E225_M0.5260.72
78_R83_R0.5250.72
86_A113_L0.5240.72
26_R55_R0.5230.71
45_L86_A0.5210.71
12_G130_D0.5190.71
212_K219_V0.5180.71
155_R159_S0.5180.71
155_R209_G0.5170.71
48_D85_V0.5170.71
15_P142_T0.5170.71
220_A231_L0.5170.71
111_M114_T0.5160.70
19_A62_I0.5160.70
144_L181_A0.5150.70
270_A274_D0.5150.70
7_V69_L0.5130.70
60_D216_H0.5120.70
209_G216_H0.5120.70
130_D170_M0.5120.70
59_S209_G0.5100.70
26_R30_T0.5100.70
11_G103_G0.5090.69
23_G138_I0.5090.69
189_F217_A0.5070.69
134_T286_G0.5060.69
73_R105_G0.5060.69
105_G108_M0.5050.69
72_A106_S0.5050.69
134_T268_M0.5020.68
287_I292_L0.5010.68
189_F201_D0.5010.68
24_V101_I0.5010.68
111_M301_I0.5000.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1pfkA 3 0.9969 100 0.032 Contact Map
1zxxA 3 0.9938 100 0.033 Contact Map
4i7eA 3 0.9938 100 0.034 Contact Map
3o8oB 1 1 100 0.056 Contact Map
4xz2A 3 0.9812 100 0.063 Contact Map
3hnoA 2 0.9875 100 0.064 Contact Map
3opyB 2 0.9969 100 0.067 Contact Map
3o8oA 1 0.9969 100 0.067 Contact Map
3opyA 2 0.9969 100 0.072 Contact Map
2higA 3 0.975 100 0.104 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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