GREMLIN Database
KEFF - Glutathione-regulated potassium-efflux system ancillary protein KefF
UniProt: P0A754 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11568
Length: 176 (165)
Sequences: 3762 (2747)
Seq/√Len: 213.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
31_E55_R3.9101.00
76_L80_K2.5671.00
7_A73_L2.4891.00
33_R52_A2.4741.00
34_S37_Q2.4341.00
50_Q86_W2.2951.00
70_I128_L2.1921.00
31_E52_A2.1851.00
6_Y17_N2.1611.00
51_E55_R2.0841.00
65_M104_V2.0741.00
99_H138_N2.0341.00
58_L99_H2.0311.00
100_L132_A2.0261.00
33_R38_L1.9861.00
18_K22_E1.9371.00
54_S86_W1.9041.00
152_D155_T1.9011.00
59_I137_L1.8911.00
47_A51_E1.8531.00
21_L25_R1.8511.00
51_E54_S1.8211.00
153_D157_E1.8171.00
75_K79_D1.7881.00
120_G123_V1.7761.00
48_A52_A1.7591.00
31_E56_A1.6681.00
3_L52_A1.6571.00
161_R164_K1.6521.00
38_L52_A1.6471.00
23_Q164_K1.6421.00
72_P76_L1.6191.00
7_A36_Y1.6131.00
49_E77_W1.5951.00
4_I21_L1.5601.00
16_A105_T1.5391.00
22_E25_R1.5381.00
78_I82_F1.5191.00
62_Q163_Y1.5161.00
155_T158_G1.5141.00
103_A163_Y1.4891.00
151_C156_L1.4081.00
33_R49_E1.3991.00
159_Q162_H1.3841.00
19_R23_Q1.3651.00
96_H136_G1.3611.00
22_E26_T1.3541.00
157_E161_R1.3351.00
48_A51_E1.3301.00
17_N32_I1.3171.00
50_Q87_A1.3171.00
46_I50_Q1.3151.00
100_L137_L1.3081.00
23_Q26_T1.3041.00
121_F144_A1.2991.00
38_L48_A1.2861.00
62_Q103_A1.2831.00
143_F162_H1.2551.00
163_Y167_L1.2441.00
36_Y73_L1.2201.00
103_A143_F1.2191.00
158_G162_H1.2071.00
45_D48_A1.2001.00
123_V126_Q1.1791.00
110_E146_H1.1751.00
19_R164_K1.1591.00
110_E114_E1.1551.00
132_A137_L1.1541.00
20_M24_A1.1341.00
145_M151_C1.1331.00
60_V101_L1.1151.00
130_A133_I1.1151.00
42_F76_L1.1131.00
161_R165_Q1.1111.00
19_R22_E1.1091.00
7_A74_L1.1091.00
165_Q168_L1.0951.00
56_A59_I1.0901.00
95_L137_L1.0891.00
21_L32_I1.0871.00
106_T146_H1.0851.00
63_H128_L1.0801.00
82_F135_C1.0751.00
97_G138_N1.0701.00
163_Y166_R1.0641.00
164_K168_L1.0641.00
89_G94_A1.0611.00
65_M121_F1.0551.00
23_Q27_L1.0491.00
38_L49_E1.0311.00
52_A55_R1.0201.00
5_I33_R1.0131.00
70_I74_L1.0091.00
23_Q168_L1.0091.00
47_A50_Q1.0081.00
63_H102_W1.0001.00
54_S94_A0.9931.00
3_L56_A0.9921.00
109_G112_H0.9801.00
57_D98_K0.9781.00
145_M159_Q0.9741.00
7_A35_L0.9691.00
9_P66_Q0.9571.00
67_W79_D0.9551.00
156_L160_A0.9420.99
29_G55_R0.9410.99
6_Y14_S0.9390.99
40_P76_L0.9260.99
15_H160_A0.9180.99
50_Q83_S0.9140.99
40_P43_N0.8950.99
26_T164_K0.8880.99
27_L168_L0.8840.99
99_H140_L0.8620.99
84_H88_Y0.8510.99
126_Q129_Q0.8510.99
14_S17_N0.8460.99
72_P80_K0.8450.99
25_R29_G0.8380.99
103_A145_M0.8350.99
78_I131_T0.8230.99
15_H153_D0.8210.99
68_Y134_Y0.8180.99
84_H90_H0.8160.99
151_C155_T0.8140.99
10_Y13_H0.8140.99
22_E164_K0.8080.98
106_T144_A0.8020.98
101_L166_R0.8020.98
23_Q167_L0.7970.98
53_L59_I0.7970.98
104_V144_A0.7950.98
104_V125_S0.7930.98
33_R77_W0.7900.98
39_Y44_I0.7850.98
15_H156_L0.7750.98
61_W132_A0.7690.98
158_G161_R0.7660.98
58_L101_L0.7620.98
106_T121_F0.7620.98
9_P13_H0.7610.98
5_I77_W0.7510.98
17_N62_Q0.7510.98
19_R160_A0.7420.97
101_L143_F0.7360.97
141_P166_R0.7330.97
156_L159_Q0.7310.97
4_I60_V0.7300.97
20_M103_A0.7290.97
13_H76_L0.7200.97
65_M124_L0.7180.97
63_H104_V0.7180.97
65_M113_F0.7180.97
67_W88_Y0.7170.97
90_H93_T0.7160.97
49_E52_A0.7130.97
119_P125_S0.7100.97
53_L61_W0.7060.96
106_T113_F0.7020.96
53_L77_W0.6970.96
7_A34_S0.6950.96
152_D156_L0.6940.96
5_I61_W0.6870.96
160_A163_Y0.6870.96
20_M163_Y0.6850.96
118_H123_V0.6810.96
102_W128_L0.6800.96
100_L139_W0.6770.95
63_H125_S0.6710.95
28_E58_L0.6700.95
11_P37_Q0.6700.95
124_L130_A0.6700.95
40_P44_I0.6690.95
162_H165_Q0.6670.95
131_T135_C0.6660.95
24_A163_Y0.6490.94
85_G89_G0.6480.94
113_F117_A0.6470.94
132_A139_W0.6450.94
63_H74_L0.6430.94
50_Q81_V0.6400.94
68_Y130_A0.6380.94
42_F83_S0.6310.93
40_P80_K0.6270.93
108_G147_C0.6240.93
160_A164_K0.6230.93
126_Q130_A0.6210.93
73_L77_W0.6200.93
45_D49_E0.6180.93
70_I78_I0.6170.93
53_L82_F0.6160.93
3_L33_R0.6150.93
154_E158_G0.6150.93
121_F125_S0.6130.92
44_I48_A0.6080.92
63_H121_F0.6030.92
71_P79_D0.6020.92
99_H136_G0.6010.92
143_F166_R0.5950.91
123_V129_Q0.5950.91
16_A103_A0.5940.91
4_I20_M0.5940.91
93_T96_H0.5930.91
14_S18_K0.5880.91
129_Q142_P0.5850.91
19_R157_E0.5850.91
117_A123_V0.5800.90
47_A158_G0.5790.90
63_H71_P0.5760.90
15_H19_R0.5750.90
20_M145_M0.5750.90
119_P123_V0.5740.90
143_F163_Y0.5730.90
20_M167_L0.5730.90
6_Y11_P0.5700.89
16_A159_Q0.5690.89
6_Y21_L0.5650.89
148_T156_L0.5610.89
43_N76_L0.5610.89
89_G92_G0.5600.89
43_N80_K0.5580.88
80_K83_S0.5570.88
68_Y108_G0.5520.88
153_D156_L0.5500.88
160_A167_L0.5480.88
87_A95_L0.5460.87
51_E165_Q0.5450.87
104_V121_F0.5450.87
74_L78_I0.5440.87
121_F128_L0.5430.87
50_Q84_H0.5390.87
151_C159_Q0.5390.87
30_V35_L0.5350.86
47_A83_S0.5310.86
63_H70_I0.5310.86
61_W74_L0.5250.85
16_A28_E0.5250.85
95_L136_G0.5210.85
161_R168_L0.5190.85
4_I30_V0.5190.85
119_P126_Q0.5190.85
99_H139_W0.5180.85
9_P36_Y0.5090.83
129_Q132_A0.5060.83
120_G126_Q0.5060.83
147_C150_I0.5050.83
48_A150_I0.5020.83
108_G113_F0.5000.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1d4aA 2 1 100 0.394 Contact Map
4gi5A 2 1 100 0.417 Contact Map
3l9wA 2 0.9886 100 0.423 Contact Map
2amjA 2 0.9205 100 0.44 Contact Map
4fglA 2 1 100 0.463 Contact Map
3rpeA 2 0.9432 100 0.465 Contact Map
3f2vA 2 0.9489 100 0.465 Contact Map
3lcmA 2 0.9773 100 0.467 Contact Map
3ha2A 2 0.9205 100 0.472 Contact Map
4r81A 4 0.983 100 0.493 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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