KEFF - ECOLI - GREMLIN Contacts

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KEFF - Glutathione-regulated potassium-efflux system ancillary protein KefF

UniProt:	P0A754	Sequences: MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAHHG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11568
Length:	176 (165)
Sequences:	3762 (2747)
Seq/√Len:	213.8

ECOLI - KEFF KEFG YCAK MDAB AZOR
BACSU - YDEQ GS14 YRKL AZOR2 AZOR1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
31_E	55_R	3.910	1.00
76_L	80_K	2.567	1.00
7_A	73_L	2.489	1.00
33_R	52_A	2.474	1.00
34_S	37_Q	2.434	1.00
50_Q	86_W	2.295	1.00
70_I	128_L	2.192	1.00
31_E	52_A	2.185	1.00
6_Y	17_N	2.161	1.00
51_E	55_R	2.084	1.00
65_M	104_V	2.074	1.00
99_H	138_N	2.034	1.00
58_L	99_H	2.031	1.00
100_L	132_A	2.026	1.00
33_R	38_L	1.986	1.00
18_K	22_E	1.937	1.00
54_S	86_W	1.904	1.00
152_D	155_T	1.901	1.00
59_I	137_L	1.891	1.00
47_A	51_E	1.853	1.00
21_L	25_R	1.851	1.00
51_E	54_S	1.821	1.00
153_D	157_E	1.817	1.00
75_K	79_D	1.788	1.00
120_G	123_V	1.776	1.00
48_A	52_A	1.759	1.00
31_E	56_A	1.668	1.00
3_L	52_A	1.657	1.00
161_R	164_K	1.652	1.00
38_L	52_A	1.647	1.00
23_Q	164_K	1.642	1.00
72_P	76_L	1.619	1.00
7_A	36_Y	1.613	1.00
49_E	77_W	1.595	1.00
4_I	21_L	1.560	1.00
16_A	105_T	1.539	1.00
22_E	25_R	1.538	1.00
78_I	82_F	1.519	1.00
62_Q	163_Y	1.516	1.00
155_T	158_G	1.514	1.00
103_A	163_Y	1.489	1.00
151_C	156_L	1.408	1.00
33_R	49_E	1.399	1.00
159_Q	162_H	1.384	1.00
19_R	23_Q	1.365	1.00
96_H	136_G	1.361	1.00
22_E	26_T	1.354	1.00
157_E	161_R	1.335	1.00
48_A	51_E	1.330	1.00
17_N	32_I	1.317	1.00
50_Q	87_A	1.317	1.00
46_I	50_Q	1.315	1.00
100_L	137_L	1.308	1.00
23_Q	26_T	1.304	1.00
121_F	144_A	1.299	1.00
38_L	48_A	1.286	1.00
62_Q	103_A	1.283	1.00
143_F	162_H	1.255	1.00
163_Y	167_L	1.244	1.00
36_Y	73_L	1.220	1.00
103_A	143_F	1.219	1.00
158_G	162_H	1.207	1.00
45_D	48_A	1.200	1.00
123_V	126_Q	1.179	1.00
110_E	146_H	1.175	1.00
19_R	164_K	1.159	1.00
110_E	114_E	1.155	1.00
132_A	137_L	1.154	1.00
20_M	24_A	1.134	1.00
145_M	151_C	1.133	1.00
60_V	101_L	1.115	1.00
130_A	133_I	1.115	1.00
42_F	76_L	1.113	1.00
161_R	165_Q	1.111	1.00
19_R	22_E	1.109	1.00
7_A	74_L	1.109	1.00
165_Q	168_L	1.095	1.00
56_A	59_I	1.090	1.00
95_L	137_L	1.089	1.00
21_L	32_I	1.087	1.00
106_T	146_H	1.085	1.00
63_H	128_L	1.080	1.00
82_F	135_C	1.075	1.00
97_G	138_N	1.070	1.00
163_Y	166_R	1.064	1.00
164_K	168_L	1.064	1.00
89_G	94_A	1.061	1.00
65_M	121_F	1.055	1.00
23_Q	27_L	1.049	1.00
38_L	49_E	1.031	1.00
52_A	55_R	1.020	1.00
5_I	33_R	1.013	1.00
70_I	74_L	1.009	1.00
23_Q	168_L	1.009	1.00
47_A	50_Q	1.008	1.00
63_H	102_W	1.000	1.00
54_S	94_A	0.993	1.00
3_L	56_A	0.992	1.00
109_G	112_H	0.980	1.00
57_D	98_K	0.978	1.00
145_M	159_Q	0.974	1.00
7_A	35_L	0.969	1.00
9_P	66_Q	0.957	1.00
67_W	79_D	0.955	1.00
156_L	160_A	0.942	0.99
29_G	55_R	0.941	0.99
6_Y	14_S	0.939	0.99
40_P	76_L	0.926	0.99
15_H	160_A	0.918	0.99
50_Q	83_S	0.914	0.99
40_P	43_N	0.895	0.99
26_T	164_K	0.888	0.99
27_L	168_L	0.884	0.99
99_H	140_L	0.862	0.99
84_H	88_Y	0.851	0.99
126_Q	129_Q	0.851	0.99
14_S	17_N	0.846	0.99
72_P	80_K	0.845	0.99
25_R	29_G	0.838	0.99
103_A	145_M	0.835	0.99
78_I	131_T	0.823	0.99
15_H	153_D	0.821	0.99
68_Y	134_Y	0.818	0.99
84_H	90_H	0.816	0.99
151_C	155_T	0.814	0.99
10_Y	13_H	0.814	0.99
22_E	164_K	0.808	0.98
106_T	144_A	0.802	0.98
101_L	166_R	0.802	0.98
23_Q	167_L	0.797	0.98
53_L	59_I	0.797	0.98
104_V	144_A	0.795	0.98
104_V	125_S	0.793	0.98
33_R	77_W	0.790	0.98
39_Y	44_I	0.785	0.98
15_H	156_L	0.775	0.98
61_W	132_A	0.769	0.98
158_G	161_R	0.766	0.98
58_L	101_L	0.762	0.98
106_T	121_F	0.762	0.98
9_P	13_H	0.761	0.98
5_I	77_W	0.751	0.98
17_N	62_Q	0.751	0.98
19_R	160_A	0.742	0.97
101_L	143_F	0.736	0.97
141_P	166_R	0.733	0.97
156_L	159_Q	0.731	0.97
4_I	60_V	0.730	0.97
20_M	103_A	0.729	0.97
13_H	76_L	0.720	0.97
65_M	124_L	0.718	0.97
63_H	104_V	0.718	0.97
65_M	113_F	0.718	0.97
67_W	88_Y	0.717	0.97
90_H	93_T	0.716	0.97
49_E	52_A	0.713	0.97
119_P	125_S	0.710	0.97
53_L	61_W	0.706	0.96
106_T	113_F	0.702	0.96
53_L	77_W	0.697	0.96
7_A	34_S	0.695	0.96
152_D	156_L	0.694	0.96
5_I	61_W	0.687	0.96
160_A	163_Y	0.687	0.96
20_M	163_Y	0.685	0.96
118_H	123_V	0.681	0.96
102_W	128_L	0.680	0.96
100_L	139_W	0.677	0.95
63_H	125_S	0.671	0.95
28_E	58_L	0.670	0.95
11_P	37_Q	0.670	0.95
124_L	130_A	0.670	0.95
40_P	44_I	0.669	0.95
162_H	165_Q	0.667	0.95
131_T	135_C	0.666	0.95
24_A	163_Y	0.649	0.94
85_G	89_G	0.648	0.94
113_F	117_A	0.647	0.94
132_A	139_W	0.645	0.94
63_H	74_L	0.643	0.94
50_Q	81_V	0.640	0.94
68_Y	130_A	0.638	0.94
42_F	83_S	0.631	0.93
40_P	80_K	0.627	0.93
108_G	147_C	0.624	0.93
160_A	164_K	0.623	0.93
126_Q	130_A	0.621	0.93
73_L	77_W	0.620	0.93
45_D	49_E	0.618	0.93
70_I	78_I	0.617	0.93
53_L	82_F	0.616	0.93
3_L	33_R	0.615	0.93
154_E	158_G	0.615	0.93
121_F	125_S	0.613	0.92
44_I	48_A	0.608	0.92
63_H	121_F	0.603	0.92
71_P	79_D	0.602	0.92
99_H	136_G	0.601	0.92
143_F	166_R	0.595	0.91
123_V	129_Q	0.595	0.91
16_A	103_A	0.594	0.91
4_I	20_M	0.594	0.91
93_T	96_H	0.593	0.91
14_S	18_K	0.588	0.91
129_Q	142_P	0.585	0.91
19_R	157_E	0.585	0.91
117_A	123_V	0.580	0.90
47_A	158_G	0.579	0.90
63_H	71_P	0.576	0.90
15_H	19_R	0.575	0.90
20_M	145_M	0.575	0.90
119_P	123_V	0.574	0.90
143_F	163_Y	0.573	0.90
20_M	167_L	0.573	0.90
6_Y	11_P	0.570	0.89
16_A	159_Q	0.569	0.89
6_Y	21_L	0.565	0.89
148_T	156_L	0.561	0.89
43_N	76_L	0.561	0.89
89_G	92_G	0.560	0.89
43_N	80_K	0.558	0.88
80_K	83_S	0.557	0.88
68_Y	108_G	0.552	0.88
153_D	156_L	0.550	0.88
160_A	167_L	0.548	0.88
87_A	95_L	0.546	0.87
51_E	165_Q	0.545	0.87
104_V	121_F	0.545	0.87
74_L	78_I	0.544	0.87
121_F	128_L	0.543	0.87
50_Q	84_H	0.539	0.87
151_C	159_Q	0.539	0.87
30_V	35_L	0.535	0.86
47_A	83_S	0.531	0.86
63_H	70_I	0.531	0.86
61_W	74_L	0.525	0.85
16_A	28_E	0.525	0.85
95_L	136_G	0.521	0.85
161_R	168_L	0.519	0.85
4_I	30_V	0.519	0.85
119_P	126_Q	0.519	0.85
99_H	139_W	0.518	0.85
9_P	36_Y	0.509	0.83
129_Q	132_A	0.506	0.83
120_G	126_Q	0.506	0.83
147_C	150_I	0.505	0.83
48_A	150_I	0.502	0.83
108_G	113_F	0.500	0.82

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1d4aA	2	1	100	0.394	Contact Map
4gi5A	2	1	100	0.417	Contact Map
3l9wA	2	0.9886	100	0.423	Contact Map
2amjA	2	0.9205	100	0.44	Contact Map
4fglA	2	1	100	0.463	Contact Map
3rpeA	2	0.9432	100	0.465	Contact Map
3f2vA	2	0.9489	100	0.465	Contact Map
3lcmA	2	0.9773	100	0.467	Contact Map
3ha2A	2	0.9205	100	0.472	Contact Map
4r81A	4	0.983	100	0.493	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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