GREMLIN Database
MSCL - Large-conductance mechanosensitive channel
UniProt: P0A742 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11180
Length: 136 (123)
Sequences: 2898 (1864)
Seq/√Len: 168.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_S38_A3.6411.00
5_K9_E3.3671.00
117_K120_V2.9821.00
6_E97_K2.6461.00
6_E93_F2.3701.00
90_F94_M2.3321.00
31_K34_S2.2661.00
6_E100_N2.1691.00
117_K123_T2.0921.00
30_G34_S2.0211.00
74_H77_V1.9531.00
57_F75_Y1.9321.00
17_V99_I1.9321.00
35_S39_D1.9191.00
3_I94_M1.9161.00
39_D77_V1.8711.00
116_T119_E1.8311.00
36_L40_I1.7351.00
58_A72_V1.7001.00
114_A120_V1.6981.00
19_L23_V1.6671.00
103_N106_K1.6461.00
40_I73_M1.6171.00
23_V30_G1.5711.00
3_I90_F1.5471.00
36_L78_F1.5241.00
118_E121_L1.5201.00
93_F97_K1.5001.00
116_T120_V1.4871.00
120_V123_T1.4761.00
94_M98_L1.4321.00
84_D88_V1.4311.00
31_K84_D1.4261.00
11_A89_A1.4111.00
19_L22_G1.4101.00
9_E104_R1.4031.00
115_P120_V1.3861.00
77_V81_N1.3751.00
80_Q84_D1.3701.00
58_A70_A1.3201.00
115_P123_T1.2871.00
44_P73_M1.2721.00
54_F79_I1.2431.00
124_E130_K1.2341.00
128_L131_E1.2311.00
105_K108_E1.1841.00
123_T128_L1.1711.00
103_N107_E1.1691.00
11_A86_L1.1561.00
46_G54_F1.1271.00
58_A74_H1.1261.00
34_S37_V1.1191.00
27_A31_K1.0891.00
32_I82_V1.0801.00
115_P119_E1.0721.00
124_E127_D1.0330.99
34_S84_D1.0220.99
51_G57_F1.0130.99
16_V36_L1.0080.99
119_E123_T1.0080.99
7_F89_A1.0070.99
104_R108_E1.0060.99
58_A68_I1.0060.99
39_D78_F1.0020.99
60_T71_V0.9950.99
7_F90_F0.9890.99
117_K124_E0.9840.99
115_P124_E0.9770.99
24_I95_A0.9760.99
92_I95_A0.9750.99
104_R107_E0.9520.99
122_L126_R0.9500.99
60_T72_V0.9400.99
39_D81_N0.9370.99
16_V23_V0.9350.99
13_R103_N0.9260.99
98_L102_L0.9070.98
17_V95_A0.9070.98
58_A69_P0.8750.98
46_G71_V0.8750.98
116_T121_L0.8650.98
35_S77_V0.8600.98
100_N103_N0.8560.98
39_D74_H0.8560.98
40_I78_F0.8500.98
119_E128_L0.8470.97
42_M46_G0.8330.97
15_N18_D0.8330.97
24_I91_A0.8280.97
49_I57_F0.8250.97
41_I87_I0.8120.97
47_L61_L0.8120.97
44_P48_L0.8040.97
4_I86_L0.8030.97
42_M80_Q0.7920.96
109_P113_P0.7820.96
24_I37_V0.7700.96
101_K105_K0.7680.96
118_E123_T0.7660.95
23_V27_A0.7620.95
42_M54_F0.7500.95
118_E128_L0.7490.95
54_F83_F0.7470.95
42_M45_L0.7470.95
35_S78_F0.7360.94
109_P112_A0.7310.94
44_P49_I0.7250.94
53_D56_Q0.7210.94
60_T70_A0.7200.94
86_L90_F0.7180.94
25_I92_I0.7120.93
16_V32_I0.7120.93
30_G91_A0.7100.93
59_V73_M0.7080.93
98_L101_K0.7060.93
21_V96_I0.7020.93
112_A115_P0.7010.93
121_L126_R0.6990.93
111_A114_A0.6970.93
9_E100_N0.6970.93
80_Q88_V0.6890.92
26_G99_I0.6870.92
96_I100_N0.6850.92
12_M16_V0.6840.92
93_F98_L0.6820.92
38_A42_M0.6710.91
11_A22_G0.6680.91
78_F82_V0.6680.91
58_A61_L0.6630.91
54_F80_Q0.6530.90
37_V42_M0.6520.90
117_K128_L0.6510.90
83_F87_I0.6460.89
116_T123_T0.6460.89
5_K8_R0.6370.89
30_G88_V0.6370.89
30_G33_V0.6320.88
24_I45_L0.6310.88
55_K80_Q0.6300.88
102_L106_K0.6300.88
41_I91_A0.6290.88
46_G76_G0.6290.88
46_G49_I0.6200.88
49_I52_I0.6190.87
87_I90_F0.6130.87
59_V75_Y0.6080.86
18_D26_G0.6030.86
57_F60_T0.6030.86
75_Y79_I0.5970.85
7_F11_A0.5940.85
61_L70_A0.5860.84
46_G51_G0.5860.84
101_K104_R0.5830.84
79_I83_F0.5820.84
81_N84_D0.5690.83
99_I103_N0.5680.83
21_V92_I0.5680.83
50_G55_K0.5650.82
115_P118_E0.5630.82
114_A117_K0.5620.82
95_A98_L0.5590.82
24_I88_V0.5540.81
13_R18_D0.5440.80
22_G92_I0.5420.80
41_I95_A0.5420.80
18_D22_G0.5390.79
126_R130_K0.5390.79
13_R16_V0.5390.79
26_G30_G0.5350.79
48_L51_G0.5280.78
19_L25_I0.5280.78
17_V21_V0.5270.78
58_A75_Y0.5260.78
68_I72_V0.5230.77
22_G100_N0.5220.77
120_V128_L0.5200.77
50_G54_F0.5190.77
55_K77_V0.5120.76
57_F79_I0.5100.75
113_P116_T0.5100.75
50_G80_Q0.5100.75
25_I29_F0.5060.75
52_I57_F0.5040.75
110_A114_A0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2oarA 7 0.9044 100 0.135 Contact Map
3hzqA 3 0.6765 100 0.3 Contact Map
4lkuA 3 0.2132 96.4 0.862 Contact Map
2kluA 1 0.4191 46 0.927 Contact Map
4h33A 3 0.5882 24.9 0.937 Contact Map
2j69A 1 0.6397 18.7 0.94 Contact Map
2yfvC 1 0.1838 13.6 0.944 Contact Map
3rlbA 1 0.6691 10.7 0.947 Contact Map
4rfsS 1 0.6397 10.2 0.947 Contact Map
4uotA 3 0.1471 9.8 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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