GREMLIN Database
MGSA - Methylglyoxal synthase
UniProt: P0A731 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12307
Length: 152 (120)
Sequences: 1290 (627)
Seq/√Len: 57.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
53_S62_A4.0231.00
13_H41_V3.7551.00
34_Q57_G2.4261.00
30_V37_L2.4031.00
89_F120_V2.3731.00
29_W89_F2.3041.00
44_A49_G2.2541.00
122_T126_I2.2191.00
13_H43_Y2.1291.00
32_R124_D2.0911.00
64_L79_S2.0511.00
50_N54_R2.0141.00
90_W110_T1.9551.00
40_H84_D1.9471.00
15_A74_V1.9341.00
72_Q75_G1.8921.00
77_L82_K1.8901.00
75_G104_A1.8020.99
14_I85_V1.7880.99
47_T65_S1.7860.99
63_M77_L1.7760.99
17_V74_V1.7670.99
24_Q51_L1.7550.99
64_L112_W1.6960.99
72_Q104_A1.6720.99
43_Y77_L1.6410.99
27_M51_L1.5730.98
61_N77_L1.5520.98
73_Q80_E1.5490.98
29_W124_D1.5260.98
30_V52_I1.5180.98
43_Y83_I1.4540.97
32_R120_V1.4440.97
78_I86_L1.4310.97
110_T118_T1.4300.97
17_V86_L1.4090.97
21_H94_N1.3960.97
99_D103_K1.3940.96
22_C25_M1.3920.96
49_G62_A1.3850.96
50_N62_A1.3710.96
29_W127_I1.3660.96
67_P98_H1.3230.95
46_G62_A1.2780.94
54_R62_A1.2260.93
85_V126_I1.2110.92
63_M83_I1.1910.92
31_E55_A1.1810.92
34_Q38_E1.1790.91
72_Q108_L1.1690.91
24_Q28_S1.1630.91
26_L52_I1.1290.90
35_P38_E1.1200.89
67_P71_D1.1180.89
29_W33_H1.1150.89
52_I56_T1.0950.88
13_H40_H1.0640.87
125_F129_S1.0390.85
33_H124_D1.0320.85
13_H83_I1.0150.84
33_H36_L0.9940.83
83_I113_N0.9860.82
43_Y63_M0.9780.82
106_L116_V0.9700.81
43_Y61_N0.9590.80
40_H85_V0.9500.80
53_S59_N0.9460.79
26_L37_L0.9340.78
32_R81_G0.9260.78
14_I40_H0.9180.77
51_L55_A0.9180.77
103_K110_T0.9080.76
53_S57_G0.8620.73
25_M120_V0.8570.72
98_H101_D0.8480.71
63_M74_V0.8360.70
28_S32_R0.8270.69
67_P101_D0.8250.69
78_I102_V0.8210.69
44_A52_I0.8150.68
115_P121_A0.8130.68
30_V56_T0.8080.68
67_P102_V0.8040.67
71_D98_H0.8020.67
38_E41_V0.8000.67
65_S71_D0.7950.66
40_H129_S0.7920.66
97_P111_V0.7790.65
77_L80_E0.7770.65
32_R36_L0.7750.64
90_W94_N0.7660.64
28_S31_E0.7510.62
65_S98_H0.7490.62
75_G101_D0.7450.61
37_L87_I0.7420.61
97_P102_V0.7200.59
96_V104_A0.7180.59
110_T132_F0.7140.58
29_W118_T0.7140.58
72_Q79_S0.7140.58
20_D23_K0.7130.58
117_A122_T0.7070.57
63_M129_S0.7050.57
65_S97_P0.7040.57
52_I55_A0.6980.56
109_A116_V0.6980.56
98_H102_V0.6970.56
31_E34_Q0.6910.56
36_L128_Q0.6850.55
47_T98_H0.6780.54
28_S87_I0.6750.54
27_M55_A0.6740.54
67_P93_L0.6700.53
16_L30_V0.6680.53
93_L98_H0.6650.53
90_W100_P0.6610.52
109_A112_W0.6590.52
68_M112_W0.6590.52
34_Q53_S0.6590.52
125_F128_Q0.6560.52
67_P105_L0.6460.51
40_H43_Y0.6460.51
90_W116_V0.6410.50
90_W103_K0.6360.50
78_I108_L0.6300.49
41_V59_N0.6290.49
83_I114_I0.6260.49
90_W118_T0.6260.49
20_D91_D0.6230.48
86_L109_A0.6230.48
90_W99_D0.6170.48
41_V61_N0.6110.47
90_W112_W0.6090.47
51_L54_R0.6070.46
86_L116_V0.6020.46
32_R121_A0.5940.45
33_H129_S0.5900.45
67_P111_V0.5880.44
71_D111_V0.5870.44
34_Q56_T0.5860.44
38_E54_R0.5810.44
127_I132_F0.5810.44
21_H96_V0.5670.42
117_A126_I0.5670.42
71_D97_P0.5620.42
36_L127_I0.5620.42
93_L97_P0.5570.41
34_Q59_N0.5540.41
28_S41_V0.5500.40
15_A77_L0.5490.40
73_Q112_W0.5490.40
75_G102_V0.5490.40
106_L118_T0.5490.40
67_P97_P0.5470.40
88_F98_H0.5470.40
90_W106_L0.5460.40
15_A86_L0.5450.40
17_V63_M0.5440.40
113_N126_I0.5420.39
26_L30_V0.5410.39
43_Y49_G0.5410.39
64_L80_E0.5360.39
52_I112_W0.5360.39
91_D105_L0.5340.39
113_N119_N0.5330.39
85_V125_F0.5320.38
16_L52_I0.5320.38
67_P107_R0.5290.38
56_T116_V0.5290.38
71_D101_D0.5270.38
101_D107_R0.5250.38
76_A79_S0.5200.37
73_Q121_A0.5150.37
28_S120_V0.5140.37
31_E61_N0.5120.36
100_P106_L0.5090.36
93_L96_V0.5080.36
46_G66_G0.5020.35
13_H84_D0.5010.35
53_S113_N0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1b93A 5 0.9934 100 0.404 Contact Map
1vmdA 5 1 100 0.425 Contact Map
2xw6A 5 0.8553 100 0.464 Contact Map
2yvqA 2 0.7961 99.9 0.601 Contact Map
1a9xA 3 0.9342 99.9 0.621 Contact Map
1g8mA 2 0.9079 96.2 0.892 Contact Map
3zzmA 2 0.9211 95.8 0.896 Contact Map
1zczA 2 0.8487 84.5 0.923 Contact Map
2qpqA 1 0.9671 46.1 0.942 Contact Map
2h5eA 1 0.8289 46 0.942 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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