ENGB - ECOLI - GREMLIN Contacts

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ENGB - Probable GTP-binding protein EngB

UniProt:	P0A6P7	Sequences: MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALNTLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEYLEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLNMVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11203
Length:	210 (165)
Sequences:	27241 (17064)
Seq/√Len:	1328.4

ECOLI - ENGB DER YFJP YKFA ERA MNME OBG HFLX FEOB CRFC IF2 EFTU1 EFTU2 YEEP SELB CYSN
BACSU - ENGB ERA DER MNME HFLX OBG YPBR IF2 EFTU
HALSA - ENGB Q9HMD6 Q9HQ88 Q9HMC2 Y1111 IF2P Q9HRX2 Q9HP92 IF2G Q9HPE4
SULSO - Q7LXR6 HFLX IF2P Q7LXL0 IF2G Q97W17

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
45_T	183_K	2.907	1.00
176_E	192_K	2.889	1.00
113_V	143_L	2.665	1.00
192_K	195_T	2.525	1.00
174_Q	192_K	2.241	1.00
118_I	144_L	2.238	1.00
67_L	75_R	2.210	1.00
192_K	196_W	2.189	1.00
147_A	177_T	2.108	1.00
41_S	180_S	2.078	1.00
116_M	142_V	1.941	1.00
28_E	75_R	1.926	1.00
44_N	55_T	1.910	1.00
30_A	109_L	1.895	1.00
72_D	194_D	1.876	1.00
45_T	186_V	1.798	1.00
111_G	141_L	1.786	1.00
113_V	193_L	1.771	1.00
143_L	178_F	1.727	1.00
115_L	145_T	1.716	1.00
69_E	73_G	1.676	1.00
141_L	192_K	1.674	1.00
159_L	177_T	1.661	1.00
144_L	177_T	1.651	1.00
42_A	115_L	1.645	1.00
161_M	165_A	1.623	1.00
130_I	134_V	1.547	1.00
143_L	189_L	1.543	1.00
31_F	113_V	1.528	1.00
69_E	75_R	1.486	1.00
103_L	132_W	1.479	1.00
160_N	164_E	1.433	1.00
47_T	68_F	1.429	1.00
159_L	163_R	1.421	1.00
158_Q	161_M	1.416	1.00
67_L	77_V	1.406	1.00
53_A	66_N	1.402	1.00
113_V	141_L	1.399	1.00
31_F	43_L	1.391	1.00
132_W	135_D	1.380	1.00
114_V	130_I	1.374	1.00
145_T	180_S	1.362	1.00
42_A	189_L	1.358	1.00
27_I	197_F	1.327	1.00
152_S	155_R	1.326	1.00
43_L	53_A	1.323	1.00
160_N	163_R	1.314	1.00
144_L	175_V	1.309	1.00
74_K	194_D	1.294	1.00
114_V	140_V	1.288	1.00
117_D	120_H	1.276	1.00
131_E	135_D	1.269	1.00
34_R	126_D	1.269	1.00
40_S	53_A	1.255	1.00
95_W	98_A	1.250	1.00
26_G	75_R	1.247	1.00
120_H	123_K	1.243	1.00
146_K	149_K	1.241	1.00
72_D	198_S	1.239	1.00
97_R	100_G	1.228	1.00
127_Q	165_A	1.228	1.00
185_G	188_K	1.217	1.00
158_Q	162_V	1.214	1.00
142_V	175_V	1.210	1.00
141_L	176_E	1.209	1.00
91_M	94_K	1.202	1.00
119_R	149_K	1.192	1.00
29_V	193_L	1.191	1.00
128_Q	132_W	1.186	1.00
129_M	133_A	1.180	1.00
186_V	190_R	1.173	1.00
187_D	191_Q	1.158	1.00
139_A	196_W	1.158	1.00
42_A	46_L	1.155	1.00
65_I	77_V	1.144	1.00
26_G	69_E	1.141	1.00
194_D	198_S	1.140	1.00
188_K	191_Q	1.138	1.00
29_V	111_G	1.117	1.00
151_A	154_A	1.116	1.00
56_S	61_R	1.116	1.00
174_Q	196_W	1.112	1.00
131_E	134_V	1.104	1.00
111_G	196_W	1.093	1.00
161_M	164_E	1.086	1.00
92_K	95_W	1.074	1.00
36_N	57_K	1.074	1.00
128_Q	131_E	1.071	1.00
130_I	133_A	1.061	1.00
93_R	97_R	1.051	1.00
188_K	192_K	1.049	1.00
93_R	96_Q	1.043	1.00
53_A	68_F	1.043	1.00
46_L	190_R	1.041	1.00
178_F	189_L	1.041	1.00
191_Q	195_T	1.035	1.00
100_G	104_E	1.015	1.00
190_R	194_D	1.007	1.00
190_R	193_L	0.984	1.00
54_R	61_R	0.983	1.00
118_I	147_A	0.975	1.00
44_N	53_A	0.960	1.00
174_Q	177_T	0.959	1.00
95_W	99_L	0.951	1.00
46_L	76_L	0.950	1.00
42_A	178_F	0.950	1.00
26_G	73_G	0.946	1.00
101_E	104_E	0.945	1.00
187_D	190_R	0.937	1.00
35_S	59_P	0.936	1.00
182_K	185_G	0.934	1.00
150_L	155_R	0.922	1.00
125_L	128_Q	0.921	1.00
124_D	128_Q	0.919	1.00
124_D	127_Q	0.916	1.00
109_L	112_L	0.914	1.00
97_R	101_E	0.906	1.00
159_L	162_V	0.898	1.00
189_L	193_L	0.896	1.00
116_M	144_L	0.896	1.00
141_L	196_W	0.890	1.00
31_F	42_A	0.887	1.00
94_K	98_A	0.883	1.00
43_L	68_F	0.882	1.00
122_L	127_Q	0.879	1.00
99_L	102_Y	0.879	1.00
129_M	132_W	0.877	1.00
96_Q	99_L	0.866	1.00
127_Q	131_E	0.861	1.00
147_A	184_Q	0.859	1.00
90_E	95_W	0.851	1.00
29_V	76_L	0.838	1.00
29_V	74_K	0.837	1.00
120_H	126_D	0.833	1.00
183_K	186_V	0.831	1.00
65_I	105_K	0.827	1.00
94_K	97_R	0.823	1.00
176_E	188_K	0.821	1.00
126_D	130_I	0.819	1.00
117_D	146_K	0.818	1.00
70_V	190_R	0.817	1.00
30_A	79_L	0.814	1.00
141_L	174_Q	0.814	1.00
191_Q	194_D	0.813	1.00
96_Q	100_G	0.812	1.00
82_Y	102_Y	0.808	1.00
118_I	150_L	0.805	1.00
34_R	125_L	0.802	1.00
28_E	110_Q	0.790	1.00
42_A	143_L	0.788	1.00
32_A	106_R	0.784	1.00
107_Q	136_S	0.784	1.00
126_D	129_M	0.779	1.00
28_E	77_V	0.776	1.00
99_L	103_L	0.765	1.00
140_V	173_V	0.762	1.00
111_G	139_A	0.760	1.00
113_V	189_L	0.757	1.00
34_R	120_H	0.753	1.00
41_S	55_T	0.751	1.00
45_T	180_S	0.745	1.00
45_T	178_F	0.742	1.00
147_A	155_R	0.738	1.00
114_V	133_A	0.735	1.00
114_V	142_V	0.735	1.00
178_F	186_V	0.732	1.00
42_A	186_V	0.731	1.00
196_W	199_E	0.730	1.00
117_D	145_T	0.725	1.00
67_L	108_S	0.725	1.00
70_V	74_K	0.722	1.00
28_E	108_S	0.722	1.00
101_E	105_K	0.713	1.00
83_G	95_W	0.707	1.00
133_A	140_V	0.705	1.00
130_I	140_V	0.703	1.00
104_E	107_Q	0.693	1.00
92_K	96_Q	0.693	1.00
111_G	193_L	0.690	1.00
32_A	112_L	0.682	1.00
151_A	177_T	0.681	1.00
47_T	70_V	0.675	1.00
143_L	176_E	0.674	1.00
142_V	173_V	0.673	1.00
42_A	180_S	0.671	1.00
163_R	175_V	0.670	1.00
122_L	126_D	0.667	1.00
49_Q	68_F	0.656	1.00
122_L	125_L	0.652	1.00
70_V	76_L	0.651	1.00
41_S	178_F	0.643	1.00
174_Q	195_T	0.641	1.00
28_E	67_L	0.639	1.00
195_T	198_S	0.636	1.00
155_R	177_T	0.629	1.00
123_K	127_Q	0.626	1.00
98_A	101_E	0.619	1.00
92_K	97_R	0.619	1.00
89_E	92_K	0.618	1.00
90_E	93_R	0.618	1.00
83_G	99_L	0.617	1.00
89_E	93_R	0.607	1.00
31_F	53_A	0.606	1.00
90_E	94_K	0.602	1.00
45_T	181_L	0.601	1.00
95_W	102_Y	0.592	1.00
112_L	138_I	0.591	1.00
77_V	105_K	0.579	1.00
185_G	189_L	0.578	1.00
181_L	184_Q	0.577	1.00
91_M	95_W	0.576	1.00
23_S	26_G	0.576	1.00
165_A	170_N	0.573	1.00
43_L	47_T	0.570	1.00
151_A	155_R	0.564	1.00
62_T	65_I	0.563	1.00
149_K	152_S	0.561	1.00
48_N	190_R	0.561	1.00
195_T	199_E	0.556	1.00
162_V	166_V	0.554	1.00
136_S	139_A	0.554	1.00
129_M	134_V	0.552	1.00
172_D	175_V	0.544	1.00
37_A	117_D	0.540	1.00
115_L	178_F	0.540	1.00
92_K	102_Y	0.539	1.00
37_A	40_S	0.535	1.00
176_E	185_G	0.535	1.00
115_L	180_S	0.533	1.00
65_I	79_L	0.533	1.00
53_A	56_S	0.533	1.00
43_L	76_L	0.530	1.00
64_L	80_P	0.528	1.00
34_R	123_K	0.528	1.00
82_Y	99_L	0.526	1.00
30_A	112_L	0.522	1.00
80_P	98_A	0.521	1.00
54_R	58_T	0.520	1.00
54_R	66_N	0.519	1.00
74_K	197_F	0.516	1.00
106_R	112_L	0.514	1.00
63_Q	91_M	0.514	1.00
178_F	181_L	0.514	1.00
97_R	104_E	0.513	1.00
147_A	150_L	0.502	1.00
78_D	81_G	0.502	1.00
40_S	55_T	0.502	1.00
112_L	133_A	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1puiA	1	0.8095	100	0.336	Contact Map
1sviA	1	0.8667	100	0.363	Contact Map
3ievA	1	0.8619	100	0.367	Contact Map
1mkyA	1	0.7571	100	0.371	Contact Map
3j65o	1	0.9381	100	0.378	Contact Map
1egaA	2	0.8524	100	0.38	Contact Map
3j8gX	1	0.9095	100	0.38	Contact Map
3pqcA	1	0.9238	100	0.383	Contact Map
2j69A	1	0.9429	100	0.388	Contact Map
4dcuA	1	0.9429	100	0.39	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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