GREMLIN Database
ISCS - Cysteine desulfurase IscS
UniProt: P0A6B7 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12677
Length: 404 (344)
Sequences: 26730 (18083)
Seq/√Len: 975.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
276_R367_D3.6151.00
280_G370_I3.4001.00
276_R363_E3.3621.00
152_H184_S3.3511.00
96_H122_T3.3281.00
167_G177_Y3.1221.00
57_Q257_R3.0311.00
162_D165_A3.0251.00
138_E169_M2.8421.00
181_A200_M2.8161.00
101_K125_A2.6701.00
254_E257_R2.5461.00
202_F215_A2.4891.00
96_H142_R2.4721.00
191_D194_Q2.4561.00
138_E172_A2.4061.00
245_P248_Q2.3891.00
96_H120_E2.2781.00
24_M28_T2.2381.00
148_V170_C2.2171.00
170_C175_I2.2021.00
284_I374_R2.1961.00
280_G367_D2.1811.00
164_A194_Q2.1711.00
276_R280_G2.1641.00
63_G191_D2.1561.00
136_E139_A2.1521.00
355_F369_T2.1461.00
272_G363_E2.1391.00
17_P21_E2.1311.00
96_H145_T2.1121.00
280_G283_D2.0261.00
318_K375_K1.9761.00
290_N304_S1.9431.00
79_D106_A1.9301.00
20_A24_M1.9171.00
136_E140_A1.9061.00
122_T140_A1.8881.00
288_Y304_S1.8881.00
17_P28_T1.8711.00
95_K144_D1.8661.00
127_Q131_I1.8581.00
125_A136_E1.8581.00
168_E172_A1.8441.00
150_I166_I1.8391.00
73_S77_E1.7991.00
57_Q253_G1.7901.00
164_A168_E1.7791.00
368_Y372_L1.7741.00
133_D136_E1.7661.00
60_D260_K1.7471.00
318_K372_L1.7381.00
20_A28_T1.7271.00
179_V197_V1.7151.00
68_E220_R1.7141.00
170_C177_Y1.6531.00
288_Y306_N1.6391.00
134_L169_M1.6271.00
72_T78_S1.6201.00
277_L357_L1.6101.00
204_G252_M1.6081.00
197_V200_M1.5891.00
96_H144_D1.5751.00
269_R273_L1.5271.00
79_D178_H1.5271.00
302_N352_S1.5261.00
21_E25_Q1.5161.00
23_M251_G1.5101.00
57_Q260_K1.5051.00
95_K146_I1.5041.00
75_A182_T1.4911.00
113_Q231_H1.4831.00
165_A168_E1.4731.00
82_A199_L1.4721.00
141_M169_M1.4651.00
153_V297_A1.4591.00
275_N279_N1.4551.00
84_K231_H1.4521.00
22_K254_E1.4471.00
169_M173_R1.4421.00
171_R198_D1.4411.00
267_M271_R1.4391.00
21_E24_M1.4241.00
171_R196_K1.4191.00
147_L178_H1.4091.00
280_G374_R1.4061.00
207_I252_M1.3991.00
65_D220_R1.3991.00
22_K25_Q1.3911.00
32_T46_Q1.3901.00
101_K123_Y1.3861.00
135_K138_E1.3821.00
264_A268_E1.3771.00
163_I179_V1.3751.00
282_K293_L1.3611.00
99_T111_C1.3591.00
53_I57_Q1.3571.00
54_A253_G1.3511.00
271_R275_N1.3501.00
168_E196_K1.3471.00
278_W301_L1.3311.00
124_L140_A1.3301.00
210_P251_G1.3281.00
363_E367_D1.3261.00
134_L165_A1.3181.00
199_L218_V1.3131.00
56_N60_D1.3091.00
274_R278_W1.3061.00
127_Q133_D1.2871.00
98_I140_A1.2851.00
138_E173_R1.2841.00
169_M172_A1.2751.00
135_K139_A1.2751.00
50_A250_V1.2741.00
167_G196_K1.2721.00
99_T123_Y1.2581.00
86_A176_I1.2561.00
208_Y358_G1.2451.00
18_R258_I1.2441.00
98_I145_T1.2381.00
10_S300_I1.2371.00
309_E312_S1.2361.00
16_D258_I1.2351.00
277_L366_I1.2341.00
53_I253_G1.2291.00
120_E142_R1.2231.00
67_R220_R1.2231.00
184_S200_M1.2211.00
268_E272_G1.2151.00
61_L185_V1.2131.00
78_S201_S1.2121.00
185_V202_F1.2081.00
153_V184_S1.2081.00
372_L375_K1.2051.00
262_E265_T1.2041.00
322_V353_I1.1971.00
148_V175_I1.1951.00
129_N292_D1.1951.00
362_T365_E1.1931.00
94_G146_I1.1901.00
137_L166_I1.1741.00
15_V20_A1.1731.00
368_Y371_E1.1721.00
58_I215_A1.1711.00
150_I177_Y1.1711.00
137_L148_V1.1711.00
278_W282_K1.1651.00
150_I163_I1.1631.00
49_E53_I1.1611.00
63_G193_S1.1561.00
370_I374_R1.1521.00
69_I217_Y1.1331.00
316_A376_S1.1321.00
160_V188_L1.1321.00
273_L366_I1.1231.00
56_N59_A1.1201.00
134_L138_E1.1191.00
102_T161_Q1.1181.00
51_V249_I1.1111.00
279_N282_K1.1061.00
152_H200_M1.1061.00
56_N66_P1.1031.00
142_R145_T1.1011.00
167_G171_R1.0941.00
280_G371_E1.0921.00
192_L217_Y1.0881.00
189_P264_A1.0871.00
279_N283_D1.0771.00
178_H201_S1.0761.00
283_D374_R1.0691.00
149_S178_H1.0611.00
97_I111_C1.0571.00
253_G257_R1.0561.00
138_E142_R1.0461.00
57_Q60_D1.0321.00
303_V355_F1.0311.00
87_A119_F1.0301.00
112_R115_E1.0291.00
262_E266_E1.0281.00
176_I199_L1.0161.00
171_R177_Y1.0071.00
371_E375_K1.0071.00
138_E165_A1.0051.00
286_E306_N1.0041.00
61_L256_Y1.0041.00
109_D112_R1.0001.00
153_V298_P0.9981.00
269_R272_G0.9961.00
141_M145_T0.9961.00
84_K113_Q0.9871.00
152_H195_L0.9851.00
367_D371_E0.9821.00
265_T268_E0.9791.00
277_L369_T0.9761.00
254_E258_I0.9751.00
10_S356_S0.9741.00
134_L137_L0.9711.00
108_L123_Y0.9651.00
65_D68_E0.9551.00
257_R261_E0.9531.00
58_I256_Y0.9461.00
142_R173_R0.9461.00
198_D219_R0.9441.00
107_V149_S0.9431.00
191_D195_L0.9371.00
170_C173_R0.9361.00
274_R301_L0.9341.00
83_I111_C0.9331.00
148_V166_I0.9321.00
62_V202_F0.9221.00
278_W289_L0.9171.00
277_L301_L0.9151.00
357_L366_I0.9151.00
62_V217_Y0.9141.00
42_R46_Q0.9121.00
364_E367_D0.9111.00
265_T269_R0.9061.00
290_N352_S0.9051.00
290_N302_N0.9021.00
314_I324_S0.8981.00
204_G213_I0.8981.00
20_A27_M0.8951.00
266_E359_R0.8941.00
113_Q116_R0.8921.00
19_V255_A0.8871.00
65_D193_S0.8861.00
266_E269_R0.8861.00
86_A90_Y0.8861.00
304_S351_S0.8811.00
192_L195_L0.8781.00
361_T366_I0.8781.00
95_K120_E0.8771.00
207_I256_Y0.8751.00
151_M155_N0.8751.00
266_E270_L0.8691.00
43_F321_A0.8661.00
213_I249_I0.8631.00
320_L372_L0.8521.00
122_T142_R0.8521.00
59_A64_A0.8451.00
277_L355_F0.8431.00
152_H190_I0.8431.00
213_I248_Q0.8401.00
312_S316_A0.8401.00
204_G209_G0.8371.00
125_A140_A0.8351.00
54_A57_Q0.8301.00
170_C174_G0.8281.00
364_E368_Y0.8241.00
86_A199_L0.8211.00
164_A195_L0.8181.00
152_H163_I0.8181.00
320_L369_T0.8161.00
91_Q95_K0.8151.00
53_I257_R0.8141.00
190_I194_Q0.8121.00
297_A300_I0.8111.00
273_L363_E0.8101.00
320_L368_Y0.8101.00
178_H199_L0.8091.00
277_L370_I0.8011.00
84_K229_Q0.7991.00
64_A68_E0.7981.00
131_I161_Q0.7961.00
46_Q49_E0.7961.00
79_D201_S0.7951.00
82_A216_L0.7921.00
23_M27_M0.7921.00
284_I287_V0.7911.00
101_K126_P0.7871.00
185_V207_I0.7861.00
185_V256_Y0.7751.00
313_L373_V0.7671.00
100_S150_I0.7651.00
167_G195_L0.7631.00
70_V81_L0.7591.00
181_A185_V0.7551.00
281_I303_V0.7541.00
369_T373_V0.7531.00
141_M175_I0.7531.00
62_V192_L0.7481.00
26_F50_A0.7481.00
189_P194_Q0.7481.00
98_I122_T0.7481.00
280_G284_I0.7471.00
287_V305_F0.7451.00
185_V190_I0.7411.00
18_R261_E0.7411.00
284_I370_I0.7401.00
54_A249_I0.7381.00
312_S315_M0.7381.00
219_R222_P0.7361.00
189_P192_L0.7361.00
64_A217_Y0.7331.00
278_W293_L0.7321.00
61_L260_K0.7311.00
27_M247_H0.7301.00
137_L141_M0.7291.00
276_R366_I0.7291.00
299_N302_N0.7281.00
141_M173_R0.7271.00
282_K292_D0.7261.00
208_Y359_R0.7231.00
57_Q256_Y0.7171.00
227_E231_H0.7141.00
12_T33_F0.7141.00
18_R254_E0.7131.00
176_I198_D0.7131.00
196_K219_R0.7111.00
361_T365_E0.7111.00
112_R123_Y0.7091.00
151_M161_Q0.7081.00
99_T149_S0.7071.00
122_T145_T0.7061.00
13_T208_Y0.7051.00
297_A301_L0.7021.00
371_E374_R0.7001.00
58_I71_F0.7001.00
369_T372_L0.7001.00
97_I147_L0.6991.00
25_Q32_T0.6991.00
257_R260_K0.6971.00
189_P267_M0.6891.00
25_Q28_T0.6881.00
99_T121_V0.6871.00
301_L304_S0.6841.00
51_V246_V0.6841.00
18_R21_E0.6811.00
272_G276_R0.6781.00
152_H160_V0.6781.00
313_L376_S0.6761.00
184_S188_L0.6751.00
140_A143_D0.6751.00
50_A53_I0.6751.00
358_G361_T0.6741.00
113_Q117_E0.6741.00
127_Q136_E0.6731.00
57_Q61_L0.6721.00
229_Q233_G0.6721.00
229_Q232_G0.6711.00
241_S244_L0.6691.00
163_I177_Y0.6681.00
134_L166_I0.6661.00
269_R363_E0.6651.00
92_K95_K0.6651.00
48_E52_D0.6641.00
317_L322_V0.6641.00
21_E28_T0.6631.00
304_S348_L0.6621.00
68_E193_S0.6621.00
75_A79_D0.6601.00
17_P20_A0.6601.00
190_I195_L0.6601.00
41_H44_G0.6581.00
55_R236_E0.6561.00
68_E219_R0.6561.00
176_I222_P0.6551.00
111_C121_V0.6541.00
230_M233_G0.6541.00
268_E271_R0.6511.00
215_A252_M0.6491.00
270_L299_N0.6481.00
16_D359_R0.6461.00
278_W297_A0.6461.00
192_L196_K0.6451.00
120_E144_D0.6441.00
22_K26_F0.6431.00
111_C149_S0.6421.00
182_T203_S0.6411.00
166_I177_Y0.6401.00
22_K250_V0.6391.00
322_V355_F0.6391.00
37_A244_L0.6351.00
16_D19_V0.6341.00
82_A178_H0.6331.00
167_G197_V0.6321.00
367_D370_I0.6321.00
181_A184_S0.6311.00
277_L281_I0.6311.00
53_I56_N0.6281.00
124_L136_E0.6281.00
228_A231_H0.6281.00
105_K109_D0.6281.00
167_G179_V0.6231.00
99_T103_E0.6211.00
62_V185_V0.6161.00
18_R262_E0.6161.00
77_E230_M0.6141.00
222_P225_R0.6131.00
165_A169_M0.6131.00
206_K354_R0.6111.00
17_P24_M0.6101.00
108_L112_R0.6091.00
228_A232_G0.6091.00
152_H179_V0.6081.00
114_L119_F0.6071.00
38_S41_H0.6011.00
168_E171_R0.5981.00
91_Q119_F0.5981.00
34_G37_A0.5961.00
52_D55_R0.5941.00
80_N84_K0.5931.00
259_A266_E0.5921.00
75_A201_S0.5921.00
314_I317_L0.5891.00
153_V160_V0.5871.00
138_E141_M0.5871.00
263_M267_M0.5841.00
296_G300_I0.5811.00
77_E81_L0.5811.00
241_S246_V0.5801.00
76_T80_N0.5791.00
231_H234_G0.5791.00
325_G328_C0.5791.00
259_A263_M0.5781.00
298_P301_L0.5761.00
300_I304_S0.5761.00
74_G214_G0.5751.00
124_L137_L0.5741.00
164_A196_K0.5731.00
102_T126_P0.5721.00
14_P33_F0.5721.00
320_L323_S0.5721.00
264_A267_M0.5721.00
276_R370_I0.5711.00
300_I356_S0.5671.00
277_L280_G0.5671.00
314_I322_V0.5671.00
242_G247_H0.5661.00
221_K224_V0.5661.00
299_N303_V0.5661.00
129_N293_L0.5651.00
19_V23_M0.5641.00
240_R246_V0.5631.00
15_V27_M0.5621.00
59_A66_P0.5611.00
82_A86_A0.5601.00
308_V313_L0.5601.00
167_G170_C0.5601.00
25_Q50_A0.5591.00
114_L117_E0.5591.00
281_I287_V0.5591.00
298_P303_V0.5581.00
26_F250_V0.5571.00
204_G207_I0.5571.00
311_E315_M0.5551.00
14_P208_Y0.5551.00
100_S103_E0.5541.00
305_F377_I0.5531.00
98_I124_L0.5521.00
281_I370_I0.5521.00
305_F373_V0.5501.00
189_P263_M0.5501.00
107_V111_C0.5481.00
131_I159_V0.5481.00
179_V200_M0.5471.00
171_R175_I0.5461.00
19_V254_E0.5461.00
327_A330_S0.5451.00
281_I373_V0.5431.00
139_A143_D0.5431.00
320_L324_S0.5431.00
276_R279_N0.5421.00
228_A233_G0.5421.00
200_M217_Y0.5411.00
280_G366_I0.5401.00
73_S76_T0.5391.00
189_P193_S0.5391.00
110_T231_H0.5381.00
60_D63_G0.5381.00
137_L169_M0.5371.00
115_E123_Y0.5361.00
253_G256_Y0.5361.00
190_I200_M0.5351.00
273_L357_L0.5351.00
230_M234_G0.5351.00
256_Y260_K0.5341.00
51_V244_L0.5341.00
137_L170_C0.5331.00
267_M270_L0.5321.00
207_I259_A0.5311.00
284_I377_I0.5291.00
141_M148_V0.5291.00
273_L361_T0.5271.00
300_I303_V0.5251.00
272_G275_N0.5251.00
198_D221_K0.5231.00
190_I193_S0.5231.00
12_T205_H0.5221.00
52_D56_N0.5211.00
16_D262_E0.5211.00
298_P302_N0.5201.00
77_E80_N0.5201.00
33_F37_A0.5171.00
220_R224_V0.5171.00
83_I110_T0.5161.00
81_L229_Q0.5151.00
199_L216_L0.5151.00
208_Y259_A0.5141.00
119_F146_I0.5131.00
258_I359_R0.5131.00
228_A234_G0.5131.00
372_L376_S0.5121.00
236_E240_R0.5111.00
326_S330_S0.5111.00
236_E243_T0.5111.00
34_G41_H0.5091.00
50_A253_G0.5081.00
26_F247_H0.5071.00
64_A192_L0.5071.00
83_I107_V0.5061.00
151_M154_N0.5061.00
305_F313_L0.5041.00
87_A91_Q0.5011.00
193_S196_K0.5001.00
55_R71_F0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lvmA 2 0.9728 100 0.382 Contact Map
3a9zA 2 0.9307 100 0.416 Contact Map
3vaxA 2 0.8663 100 0.421 Contact Map
4isyA 2 0.9257 100 0.423 Contact Map
4hvkA 2 0.9084 100 0.433 Contact Map
1eg5A 2 0.8911 100 0.433 Contact Map
3e9kA 2 0.8713 100 0.438 Contact Map
1kmjA 2 0.9059 100 0.44 Contact Map
4q75A 2 0.9183 100 0.443 Contact Map
1t3iA 2 0.9059 100 0.451 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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