GREMLIN Database
T1MK - Type I restriction enzyme EcoKI M protein
UniProt: P08957 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10458
Length: 529 (441)
Sequences: 6086 (4039)
Seq/√Len: 192.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
326_R330_D5.6671.00
159_I307_A4.2971.00
300_L306_A4.0021.00
323_D326_R3.9231.00
261_H301_H3.8911.00
293_M310_V3.4511.00
399_Q403_R3.3471.00
394_T397_H3.2841.00
53_G58_D3.2611.00
296_I306_A3.1921.00
161_H188_Y2.9271.00
478_S481_E2.9201.00
375_V435_N2.8261.00
433_E437_N2.7921.00
244_I249_T2.7331.00
436_K440_Q2.6871.00
159_I264_A2.6301.00
327_D331_K2.6041.00
75_L79_G2.5911.00
296_I308_V2.5121.00
433_E436_K2.4241.00
307_A354_V2.3951.00
12_K15_D2.3941.00
171_V185_A2.3711.00
459_K463_L2.3701.00
244_I250_L2.3421.00
431_D434_E2.2781.00
76_V79_G2.2661.00
176_A225_A2.1171.00
157_K184_E2.0981.00
335_H358_T2.0961.00
461_D464_D2.0951.00
172_Q299_T2.0681.00
307_A356_F2.0351.00
432_S440_Q1.9731.00
157_K395_D1.9681.00
244_I248_N1.9381.00
322_T326_R1.9231.00
297_I332_C1.9151.00
175_A265_T1.9081.00
176_A214_G1.8991.00
161_H402_E1.8971.00
69_Q73_K1.8851.00
297_I328_L1.8791.00
396_E399_Q1.8711.00
472_D475_D1.8671.00
179_A225_A1.8441.00
38_K42_E1.8321.00
300_L304_G1.8141.00
480_P484_V1.8121.00
265_T296_I1.8061.00
470_D474_I1.7941.00
8_A12_K1.7841.00
312_D339_R1.7781.00
411_G415_R1.7681.00
432_S436_K1.7571.00
315_L337_I1.7471.00
467_W471_K1.7381.00
329_M375_V1.7251.00
175_A292_F1.7241.00
480_P483_D1.7221.00
400_P404_V1.7201.00
471_K475_D1.7111.00
428_E434_E1.6891.00
309_V354_V1.6881.00
438_T441_H1.6841.00
471_K474_I1.6751.00
330_D436_K1.6671.00
402_E405_Y1.6661.00
248_N251_G1.6591.00
380_L401_F1.6451.00
270_G273_A1.6381.00
469_K472_D1.6181.00
445_S449_K1.6041.00
410_H415_R1.5921.00
163_L262_I1.5741.00
443_A446_R1.5561.00
403_R407_E1.5531.00
157_K161_H1.5531.00
476_A483_D1.5491.00
473_S477_D1.5371.00
71_Y78_L1.5351.00
305_R358_T1.5281.00
460_S464_D1.5171.00
45_Q57_D1.5121.00
148_Q218_V1.5081.00
477_D481_E1.5071.00
164_K188_Y1.4951.00
238_L244_I1.4921.00
443_A447_W1.4901.00
74_M77_H1.4881.00
462_S466_S1.4831.00
302_P359_K1.4811.00
297_I333_H1.4741.00
216_E221_T1.4731.00
247_G252_S1.4671.00
109_N112_S1.4661.00
464_D467_W1.4601.00
397_H400_P1.4501.00
464_D468_L1.4431.00
330_D440_Q1.4381.00
466_S470_D1.4371.00
160_I188_Y1.4321.00
184_E187_R1.4291.00
9_K112_S1.4181.00
411_G414_P1.4181.00
71_Y75_L1.4181.00
374_D431_D1.4151.00
458_A461_D1.4021.00
474_I478_S1.3941.00
434_E437_N1.3911.00
71_Y79_G1.3851.00
12_K109_N1.3551.00
85_L88_A1.3541.00
158_T162_L1.3471.00
26_N29_N1.3441.00
343_G349_G1.3411.00
442_L447_W1.3391.00
169_E262_I1.3331.00
476_A479_L1.3301.00
216_E222_R1.3291.00
458_A464_D1.3181.00
459_K467_W1.3171.00
450_F454_W1.3161.00
475_D478_S1.3111.00
400_P403_R1.3101.00
478_S482_P1.3041.00
174_P265_T1.2981.00
426_E430_A1.2841.00
156_I181_F1.2791.00
481_E485_L1.2791.00
479_L482_P1.2751.00
328_L333_H1.2741.00
332_C361_T1.2731.00
164_K406_G1.2721.00
60_K111_D1.2691.00
385_P392_P1.2671.00
57_D61_S1.2581.00
51_P54_Y1.2511.00
163_L307_A1.2421.00
160_I264_A1.2421.00
398_L402_E1.2421.00
444_T447_W1.2421.00
334_L357_F1.2291.00
312_D351_K1.2201.00
462_S465_I1.2201.00
337_I355_L1.2171.00
434_E438_T1.2111.00
408_D411_G1.2091.00
85_L89_V1.2071.00
247_G251_G1.2021.00
463_L467_W1.1971.00
395_D399_Q1.1931.00
270_G275_T1.1931.00
444_T448_R1.1921.00
66_E70_F1.1901.00
383_N400_P1.1841.00
332_C373_D1.1791.00
58_D61_S1.1731.00
467_W470_D1.1731.00
465_I468_L1.1731.00
213_I260_A1.1731.00
52_E55_R1.1711.00
461_D465_I1.1691.00
67_Q70_F1.1671.00
461_D469_K1.1671.00
458_A462_S1.1631.00
407_E411_G1.1611.00
313_N317_E1.1601.00
461_D472_D1.1601.00
342_T349_G1.1591.00
343_G351_K1.1571.00
64_G70_F1.1491.00
64_G67_Q1.1441.00
336_T356_F1.1401.00
246_L249_T1.1311.00
312_D316_F1.1271.00
90_F93_V1.1271.00
189_V210_R1.1221.00
444_T449_K1.1211.00
342_T351_K1.1101.00
383_N397_H1.1071.00
455_I458_A1.1061.00
338_L356_F1.1051.00
270_G288_K1.1051.00
440_Q446_R1.1041.00
74_M79_G1.1041.00
446_R449_K1.1031.00
270_G274_G1.0951.00
464_D472_D1.0931.00
75_L78_L1.0921.00
442_L445_S1.0921.00
174_P296_I1.0871.00
72_R76_V1.0861.00
58_D62_R1.0751.00
274_G278_T1.0731.00
424_A427_T1.0701.00
338_L354_V1.0671.00
159_I354_V1.0651.00
470_D473_S1.0621.00
310_V355_L1.0601.00
50_L56_W1.0601.00
303_G360_G1.0561.00
427_T430_A1.0551.00
172_Q249_T1.0531.00
442_L448_R1.0531.00
39_M43_T1.0511.00
52_E58_D1.0501.00
160_I185_A1.0491.00
247_G250_L1.0481.00
464_D471_K1.0451.00
404_V411_G1.0401.00
460_S463_L1.0381.00
164_K192_Q1.0301.00
348_Q351_K1.0281.00
66_E69_Q1.0281.00
477_D480_P1.0271.00
432_S435_N1.0251.00
310_V314_V1.0221.00
341_P352_T1.0211.00
170_V258_P1.0161.00
475_D479_L1.0121.00
67_Q75_L1.0121.00
313_N351_K1.0081.00
461_D468_L1.0071.00
468_L472_D1.0011.00
447_W455_I1.0011.00
305_R335_H0.9991.00
262_I305_R0.9971.00
171_V189_V0.9961.00
86_V90_F0.9940.99
310_V353_N0.9910.99
458_A472_D0.9910.99
443_A448_R0.9910.99
49_Y52_E0.9880.99
374_D429_V0.9860.99
246_L251_G0.9850.99
34_L37_L0.9820.99
259_K302_P0.9820.99
472_D476_A0.9790.99
172_Q260_A0.9790.99
446_R453_E0.9750.99
156_I180_G0.9690.99
455_I459_K0.9680.99
60_K107_V0.9670.99
458_A469_K0.9620.99
461_D471_K0.9620.99
476_A480_P0.9590.99
271_S278_T0.9590.99
172_Q263_V0.9580.99
157_K187_R0.9410.99
56_W60_K0.9410.99
289_Q311_P0.9390.99
479_L483_D0.9380.99
11_W14_C0.9370.99
104_T108_S0.9350.99
443_A454_W0.9340.99
474_I477_D0.9310.99
169_E261_H0.9290.99
376_W380_L0.9270.99
428_E431_D0.9260.99
404_V414_P0.9250.99
271_S275_T0.9230.99
23_S28_V0.9220.99
454_W461_D0.9210.99
275_T278_T0.9160.99
5_D9_K0.9130.99
29_N220_G0.9130.99
464_D475_D0.9070.99
376_W379_D0.9030.99
460_S468_L0.9010.99
62_R70_F0.8950.99
445_S448_R0.8940.99
83_K86_V0.8930.99
410_H428_E0.8920.99
399_Q402_E0.8890.99
442_L451_S0.8870.99
423_N427_T0.8870.99
430_A435_N0.8860.99
312_D341_P0.8840.99
465_I479_L0.8840.99
423_N426_E0.8790.99
410_H413_S0.8790.99
445_S450_F0.8780.99
446_R451_S0.8760.99
284_P291_C0.8730.99
89_V93_V0.8720.99
334_L355_L0.8720.99
441_H444_T0.8720.99
332_C359_K0.8710.99
440_Q447_W0.8710.99
334_L337_I0.8710.99
312_D348_Q0.8660.99
441_H446_R0.8650.99
246_L250_L0.8650.99
16_N105_A0.8650.99
54_Y77_H0.8640.99
229_C238_L0.8600.99
84_K90_F0.8590.99
407_E410_H0.8560.98
77_H80_E0.8540.98
269_F352_T0.8520.98
265_T308_V0.8510.98
266_N345_F0.8490.98
447_W451_S0.8490.98
438_T447_W0.8480.98
37_L41_K0.8470.98
16_N109_N0.8460.98
375_V378_Y0.8450.98
317_E320_K0.8430.98
425_E428_E0.8430.98
340_L350_V0.8400.98
450_F455_I0.8390.98
73_K77_H0.8380.98
244_I247_G0.8380.98
169_E305_R0.8370.98
273_A276_N0.8360.98
36_F227_M0.8360.98
465_I469_K0.8340.98
328_L334_L0.8330.98
458_A471_K0.8330.98
72_R94_S0.8320.98
468_L471_K0.8320.98
430_A434_E0.8290.98
446_R455_I0.8210.98
272_A286_S0.8210.98
68_L72_R0.8180.98
447_W454_W0.8170.98
424_A430_A0.8100.98
73_K76_V0.8090.98
289_Q310_V0.8080.98
472_D482_P0.8070.98
448_R453_E0.8070.98
440_Q444_T0.8060.98
442_L449_K0.8030.98
448_R455_I0.8020.98
311_P350_V0.8010.98
295_H298_E0.8010.98
389_K393_F0.8000.98
297_I357_F0.7990.98
63_I70_F0.7960.98
456_R459_K0.7950.98
271_S288_K0.7930.98
468_L475_D0.7920.98
179_A182_L0.7900.97
395_D402_E0.7880.97
185_A264_A0.7850.97
437_N441_H0.7840.97
408_D429_V0.7840.97
442_L446_R0.7830.97
380_L386_S0.7830.97
244_I251_G0.7810.97
308_V357_F0.7790.97
23_S218_V0.7780.97
385_P394_T0.7770.97
55_R58_D0.7750.97
306_A357_F0.7750.97
293_M355_L0.7750.97
269_F350_V0.7730.97
451_S454_W0.7720.97
446_R450_F0.7700.97
408_D414_P0.7690.97
342_T348_Q0.7690.97
340_L344_I0.7690.97
187_R232_H0.7650.97
57_D60_K0.7640.97
426_E429_V0.7610.97
448_R452_R0.7600.97
435_N438_T0.7590.97
387_F392_P0.7580.97
388_G392_P0.7580.97
96_T99_E0.7570.97
322_T439_D0.7570.97
188_Y191_S0.7550.97
452_R456_R0.7550.97
70_F73_K0.7520.97
472_D479_L0.7500.97
182_L214_G0.7490.97
187_R190_K0.7480.96
439_D446_R0.7480.96
101_K105_A0.7470.96
159_I181_F0.7470.96
162_L356_F0.7450.96
465_I472_D0.7440.96
293_M308_V0.7430.96
124_R129_D0.7420.96
125_D228_N0.7420.96
459_K465_I0.7410.96
343_G348_Q0.7400.96
186_D212_F0.7380.96
408_D413_S0.7340.96
70_F74_M0.7320.96
271_S274_G0.7310.96
314_V355_L0.7310.96
436_K439_D0.7300.96
105_A109_N0.7290.96
425_E430_A0.7280.96
458_A465_I0.7270.96
430_A433_E0.7260.96
473_S476_A0.7240.96
314_V353_N0.7230.96
427_T431_D0.7230.96
344_I348_Q0.7220.96
444_T451_S0.7210.96
133_G137_K0.7210.96
15_D18_R0.7200.96
453_E456_R0.7200.96
337_I353_N0.7170.96
469_K483_D0.7150.95
30_E33_S0.7140.95
70_F75_L0.7130.95
23_S29_N0.7110.95
156_I184_E0.7110.95
434_E447_W0.7100.95
68_L71_Y0.7090.95
294_Q327_D0.7080.95
449_K452_R0.7080.95
53_G56_W0.7070.95
29_N33_S0.7060.95
49_Y53_G0.7050.95
55_R60_K0.7050.95
153_R180_G0.7050.95
170_V211_A0.7040.95
452_R455_I0.7030.95
162_L338_L0.7020.95
28_V32_A0.7020.95
336_T405_Y0.7010.95
273_A278_T0.6990.95
465_I471_K0.6980.95
86_V89_V0.6980.95
181_F264_A0.6950.95
462_S472_D0.6930.95
441_H447_W0.6920.95
340_L345_F0.6920.95
12_K16_N0.6900.95
375_V379_D0.6890.94
42_E45_Q0.6880.94
261_H303_G0.6880.94
294_Q324_I0.6870.94
150_F221_T0.6870.94
447_W450_F0.6870.94
372_T378_Y0.6870.94
441_H445_S0.6850.94
339_R351_K0.6840.94
437_N440_Q0.6830.94
445_S452_R0.6770.94
320_K323_D0.6760.94
458_A475_D0.6760.94
170_V213_I0.6750.94
289_Q313_N0.6750.94
170_V261_H0.6740.94
154_P392_P0.6730.94
23_S144_S0.6730.94
469_K480_P0.6720.94
460_S478_S0.6720.94
448_R454_W0.6710.94
334_L375_V0.6690.94
161_H399_Q0.6690.94
309_V349_G0.6690.94
263_V300_L0.6680.94
172_Q215_L0.6650.93
423_N429_V0.6650.93
415_R427_T0.6640.93
176_A179_A0.6630.93
444_T455_I0.6600.93
41_K44_G0.6570.93
312_D342_T0.6560.93
443_A453_E0.6560.93
397_H404_V0.6550.93
79_G82_D0.6540.93
50_L54_Y0.6540.93
446_R454_W0.6530.93
462_S483_D0.6530.93
303_G358_T0.6520.93
344_I350_V0.6520.93
466_S480_P0.6510.93
158_T402_E0.6510.93
270_G278_T0.6510.93
53_G73_K0.6480.92
372_T376_W0.6480.92
398_L405_Y0.6480.92
444_T454_W0.6470.92
447_W453_E0.6460.92
313_N348_Q0.6460.92
151_T266_N0.6460.92
74_M78_L0.6460.92
11_W137_K0.6450.92
463_L466_S0.6450.92
464_D469_K0.6440.92
41_K45_Q0.6430.92
312_D350_V0.6420.92
33_S36_F0.6420.92
460_S466_S0.6410.92
466_S469_K0.6410.92
456_R460_S0.6410.92
284_P294_Q0.6400.92
463_L468_L0.6380.92
84_K87_Q0.6380.92
33_S44_G0.6360.92
406_G410_H0.6360.92
85_L90_F0.6310.91
430_A440_Q0.6300.91
439_D442_L0.6300.91
6_L130_M0.6300.91
439_D447_W0.6280.91
400_P411_G0.6280.91
319_G426_E0.6270.91
311_P348_Q0.6260.91
425_E429_V0.6260.91
68_L74_M0.6250.91
382_T386_S0.6210.91
383_N387_F0.6210.91
377_V380_L0.6200.91
36_F41_K0.6200.91
170_V301_H0.6200.91
29_N32_A0.6190.91
311_P351_K0.6180.91
78_L82_D0.6170.91
272_A291_C0.6170.91
155_L344_I0.6170.91
444_T450_F0.6150.90
221_T224_L0.6150.90
53_G57_D0.6130.90
297_I359_K0.6120.90
478_S483_D0.6100.90
435_N441_H0.6090.90
10_L130_M0.6090.90
445_S455_I0.6080.90
294_Q298_E0.6060.90
371_C376_W0.6060.90
67_Q78_L0.6050.90
59_L62_R0.6040.90
11_W18_R0.6040.90
63_I66_E0.5990.89
340_L352_T0.5950.89
5_D8_A0.5930.89
90_F94_S0.5920.89
443_A449_K0.5920.89
25_Q29_N0.5920.89
473_S481_E0.5890.88
435_N440_Q0.5890.88
380_L389_K0.5890.88
340_L348_Q0.5880.88
476_A482_P0.5870.88
471_K476_A0.5870.88
183_I229_C0.5850.88
228_N232_H0.5840.88
270_G312_D0.5830.88
313_N350_V0.5820.88
462_S476_A0.5820.88
346_Y350_V0.5820.88
449_K453_E0.5820.88
395_D398_L0.5810.88
469_K478_S0.5810.88
259_K301_H0.5790.88
153_R184_E0.5780.87
13_L106_L0.5770.87
273_A286_S0.5760.87
478_S485_L0.5750.87
342_T350_V0.5740.87
59_L107_V0.5730.87
443_A458_A0.5720.87
33_S41_K0.5710.87
70_F78_L0.5690.87
481_E484_V0.5680.87
311_P352_T0.5680.87
448_R451_S0.5680.87
26_N96_T0.5670.86
435_N439_D0.5670.86
65_Q71_Y0.5660.86
451_S455_I0.5660.86
455_I463_L0.5660.86
335_H356_F0.5640.86
439_D444_T0.5630.86
56_W71_Y0.5630.86
263_V296_I0.5630.86
385_P388_G0.5620.86
475_D482_P0.5610.86
273_A288_K0.5600.86
297_I306_A0.5600.86
375_V380_L0.5590.86
26_N31_L0.5570.86
313_N341_P0.5550.85
38_K41_K0.5530.85
105_A108_S0.5520.85
67_Q71_Y0.5510.85
9_K12_K0.5500.85
423_N428_E0.5490.85
14_C17_L0.5480.85
312_D349_G0.5470.84
458_A467_W0.5470.84
341_P351_K0.5460.84
225_A238_L0.5460.84
384_M387_F0.5450.84
317_E321_G0.5440.84
14_C27_Y0.5440.84
342_T352_T0.5440.84
7_V346_Y0.5440.84
469_K476_A0.5430.84
335_H408_D0.5430.84
463_L470_D0.5430.84
135_L224_L0.5430.84
469_K473_S0.5410.84
76_V80_E0.5380.84
83_K87_Q0.5380.84
272_A275_T0.5380.84
313_N329_M0.5370.83
461_D475_D0.5350.83
409_P415_R0.5340.83
91_H94_S0.5330.83
294_Q297_I0.5330.83
410_H414_P0.5330.83
456_R463_L0.5320.83
389_K392_P0.5310.83
50_L53_G0.5300.83
439_D448_R0.5280.82
450_F453_E0.5280.82
460_S472_D0.5280.82
56_W59_L0.5280.82
471_K478_S0.5270.82
51_P55_R0.5270.82
315_L353_N0.5270.82
313_N316_F0.5260.82
376_W383_N0.5260.82
272_A276_N0.5260.82
459_K462_S0.5250.82
95_T99_E0.5240.82
67_Q74_M0.5230.82
406_G409_P0.5220.82
406_G439_D0.5210.82
136_Q146_A0.5190.81
17_L22_V0.5190.81
449_K454_W0.5180.81
423_N431_D0.5180.81
174_P295_H0.5180.81
346_Y349_G0.5180.81
339_R353_N0.5170.81
343_G352_T0.5160.81
71_Y76_V0.5150.81
60_K108_S0.5140.81
97_I100_P0.5130.81
146_A218_V0.5130.81
182_L229_C0.5110.80
14_C137_K0.5090.80
340_L343_G0.5090.80
386_S391_T0.5090.80
319_G322_T0.5090.80
161_H191_S0.5080.80
189_V194_N0.5080.80
87_Q93_V0.5070.80
387_F390_R0.5070.80
14_C18_R0.5060.80
465_I480_P0.5060.80
287_N314_V0.5060.80
344_I349_G0.5040.79
441_H449_K0.5040.79
294_Q308_V0.5030.79
289_Q314_V0.5030.79
16_N38_K0.5030.79
438_T444_T0.5030.79
460_S471_K0.5020.79
400_P405_Y0.5000.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ar0A 1 0.8771 100 0.299 Contact Map
3lkdA 1 0.7618 100 0.354 Contact Map
3ufbA 1 0.7902 100 0.362 Contact Map
3khkA 1 0.7958 100 0.375 Contact Map
2okcA 1 0.7921 100 0.375 Contact Map
3s1sA 1 0.8904 100 0.564 Contact Map
2f8lA 1 0.5917 100 0.582 Contact Map
2ih2A 1 0.6068 100 0.695 Contact Map
3v97A 1 0.8185 99.9 0.774 Contact Map
3lduA 1 0.5879 99.8 0.802 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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