GREMLIN Database
SYFA - Phenylalanine--tRNA ligase alpha subunit
UniProt: P08312 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10709
Length: 327 (318)
Sequences: 3533 (1810)
Seq/√Len: 101.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
216_T289_V6.3631.00
135_D138_H4.1771.00
251_T254_S3.6241.00
212_L275_V3.5241.00
278_N281_R3.4631.00
20_D23_A3.1901.00
255_A272_C3.0171.00
240_Q260_M2.9751.00
137_Y142_A2.7741.00
228_H241_I2.6551.00
136_D141_D2.6451.00
14_A30_E2.6021.00
74_R77_E2.5741.00
211_G295_F2.5681.00
115_R306_R2.5361.00
261_G265_K2.5351.00
44_T47_E2.5041.00
261_G264_G2.4301.00
220_F255_A2.4001.00
49_P52_E2.3901.00
227_L270_L2.3881.00
116_I209_M2.3871.00
125_F226_T2.3491.00
10_S14_A2.3371.00
281_R287_P2.2961.00
12_K70_A2.2491.00
281_R288_E2.2461.00
285_I290_Y2.2131.00
269_V302_L2.2011.00
75_K79_E2.1831.00
19_S23_A2.1531.00
213_I218_I2.1301.00
141_D147_G2.0821.00
138_H179_K2.0701.00
10_S37_H2.0681.00
81_A85_A2.0551.00
222_N225_G1.9221.00
85_A89_A1.8771.00
228_H232_R1.8431.00
69_Q73_A1.8361.00
9_A13_A1.8281.00
30_E37_H1.7971.00
58_A62_E1.7531.00
22_A26_N1.7341.00
171_S210_E1.6691.00
116_I299_M1.6631.00
161_D164_R1.6571.00
18_A27_V1.6541.00
139_N168_T1.6171.00
199_D202_H1.6031.00
77_E81_A1.5861.00
175_I283_V1.5831.00
257_V270_L1.5571.00
106_L110_T1.5471.00
50_P53_R1.5391.00
134_E176_R1.5281.00
128_A187_I1.5261.00
13_A17_Q1.5211.00
114_D117_E1.5181.00
154_D246_S1.5091.00
119_F233_N1.5011.00
76_A80_S1.4901.00
106_L114_D1.4841.00
232_R237_E1.4611.00
184_P215_D1.4601.00
177_T181_Q1.4491.00
137_Y179_K1.4491.00
68_Q72_N1.4461.00
36_G39_T1.4421.00
262_K265_K1.4251.00
48_L56_A1.4191.00
80_S84_N1.4021.00
122_E233_N1.4021.00
203_T316_F1.3991.00
178_M185_I1.3941.00
41_Q60_I1.3891.00
15_I18_A1.3861.00
239_L260_M1.3831.00
25_D28_R1.3740.99
21_V79_E1.3690.99
56_A60_I1.3640.99
174_Q275_V1.3490.99
297_M302_L1.3330.99
197_D203_T1.3310.99
197_D204_P1.3250.99
234_F302_L1.3170.99
54_P58_A1.3130.99
40_L43_T1.3130.99
278_N282_N1.3030.99
21_V78_L1.2950.99
46_R53_R1.2900.99
205_M316_F1.2890.99
42_M60_I1.2770.99
214_V280_L1.2760.99
26_N30_E1.2730.99
230_F234_F1.2660.99
145_I169_Q1.2660.99
84_N88_A1.2650.99
9_A12_K1.2560.99
127_V188_I1.2510.99
14_A31_Y1.2480.99
11_A31_Y1.2400.99
59_V62_E1.2350.99
12_K16_S1.2270.99
145_I278_N1.2260.99
67_V71_L1.2260.99
231_L257_V1.2050.99
139_N172_G1.2050.99
15_I27_V1.2040.99
226_T293_F1.2010.99
128_A173_V1.1970.99
309_V315_F1.1960.99
18_A24_L1.1940.99
198_Y203_T1.1850.98
126_T181_Q1.1840.98
244_R258_D1.1820.98
55_A59_V1.1810.98
113_I117_E1.1740.98
111_R307_Y1.1730.98
142_A175_I1.1680.98
21_V82_A1.1670.98
219_S222_N1.1590.98
86_R90_E1.1570.98
113_I299_M1.1550.98
82_A85_A1.1550.98
137_Y141_D1.1510.98
227_L257_V1.1510.98
73_A77_E1.1450.98
23_A26_N1.1420.98
25_D75_K1.1340.98
260_M264_G1.1280.98
52_E55_A1.1220.98
62_E66_Q1.1190.98
228_H243_F1.1190.98
155_H196_N1.1110.98
270_L297_M1.1110.98
60_I63_A1.1040.98
117_E127_V1.1030.98
131_P176_R1.1000.98
123_L229_D1.0970.97
43_T46_R1.0960.97
127_V190_P1.0950.97
65_E69_Q1.0910.97
12_K66_Q1.0890.97
11_A67_V1.0860.97
128_A189_A1.0790.97
37_H40_L1.0760.97
300_E315_F1.0760.97
178_M285_I1.0710.97
56_A59_V1.0660.97
197_D201_T1.0650.97
112_T303_T1.0540.97
72_N76_A1.0530.97
171_S174_Q1.0520.97
286_D289_V1.0510.97
112_T115_R1.0400.97
269_V305_L1.0370.96
82_A86_R1.0350.96
154_D169_Q1.0310.96
142_A283_V1.0240.96
20_D82_A1.0220.96
13_A16_S1.0160.96
257_V272_C1.0150.96
232_R238_D1.0130.96
65_E68_Q1.0120.96
248_F256_E1.0070.96
41_Q44_T1.0040.96
29_V33_G1.0020.96
41_Q56_A1.0020.96
25_D29_V1.0020.96
260_M265_K0.9970.96
88_A91_T0.9970.96
83_L87_L0.9960.96
137_Y282_N0.9950.96
187_I212_L0.9930.95
37_H41_Q0.9900.95
110_T114_D0.9850.95
312_L315_F0.9750.95
117_E188_I0.9720.95
38_L63_A0.9710.95
247_Y276_H0.9690.95
160_F165_L0.9670.95
74_R81_A0.9660.95
322_F326_F0.9640.95
214_V291_S0.9570.94
181_Q185_I0.9540.94
72_N75_K0.9540.94
145_I159_W0.9530.94
136_D155_H0.9520.94
87_L90_E0.9450.94
280_L285_I0.9400.94
213_I226_T0.9360.94
112_T234_F0.9360.94
85_A88_A0.9330.94
50_P54_P0.9320.94
229_D233_N0.9250.93
112_T307_Y0.9230.93
134_E172_G0.9200.93
207_H300_E0.9110.93
38_L67_V0.9090.93
188_I209_M0.9070.93
10_S13_A0.8970.92
169_Q172_G0.8970.92
303_T307_Y0.8890.92
45_L56_A0.8860.92
61_N64_K0.8820.92
197_D202_H0.8800.92
109_V207_H0.8760.91
246_S256_E0.8740.91
40_L44_T0.8680.91
246_S258_D0.8530.90
170_T210_E0.8530.90
39_T43_T0.8530.90
81_A84_N0.8530.90
142_A179_K0.8520.90
276_H283_V0.8520.90
220_F274_M0.8500.90
132_E194_Y0.8480.90
66_Q70_A0.8460.90
70_A73_A0.8450.90
151_A155_H0.8440.90
108_P111_R0.8370.89
173_V189_A0.8370.89
145_I155_H0.8310.89
314_S317_E0.8310.89
44_T56_A0.8230.89
22_A82_A0.8230.89
143_L175_I0.8200.88
209_M300_E0.8200.88
39_T42_M0.8180.88
69_Q72_N0.8150.88
310_T314_S0.8140.88
217_N289_V0.8100.88
116_I188_I0.8080.88
198_Y205_M0.8070.88
70_A74_R0.8060.88
169_Q196_N0.8030.87
48_L52_E0.8020.87
130_G173_V0.8000.87
244_R256_E0.7990.87
28_R75_K0.7950.87
217_N291_S0.7870.86
12_K15_I0.7840.86
198_Y204_P0.7830.86
147_G163_T0.7800.86
322_F325_Q0.7780.86
125_F293_F0.7710.85
78_L82_A0.7700.85
154_D247_Y0.7700.85
11_A15_I0.7680.85
165_L168_T0.7660.85
128_A177_T0.7630.85
241_I257_V0.7580.84
136_D154_D0.7560.84
11_A18_A0.7560.84
160_F163_T0.7550.84
178_M212_L0.7540.84
17_Q23_A0.7420.83
177_T187_I0.7400.83
280_L287_P0.7390.83
42_M64_K0.7380.83
46_R50_P0.7360.82
274_M293_F0.7330.82
66_Q69_Q0.7300.82
179_K283_V0.7290.82
20_D24_L0.7250.81
76_A79_E0.7250.81
177_T185_I0.7210.81
106_L111_R0.7210.81
312_L316_F0.7200.81
159_W196_N0.7200.81
15_I28_R0.7140.80
114_D118_S0.7110.80
203_T317_E0.7100.80
81_A89_A0.7100.80
79_E82_A0.7090.80
136_D151_A0.7090.80
145_I151_A0.7090.80
207_H316_F0.7060.80
320_L324_K0.7050.80
83_L86_R0.7030.79
275_V292_G0.7020.79
109_V205_M0.6960.79
16_S77_E0.6960.79
216_T290_Y0.6950.79
182_Q289_V0.6950.79
21_V25_D0.6940.79
248_F268_E0.6930.78
74_R78_L0.6900.78
116_I297_M0.6880.78
14_A17_Q0.6860.78
62_E65_E0.6860.78
276_H281_R0.6840.78
215_D290_Y0.6810.77
178_M283_V0.6800.77
231_L270_L0.6800.77
177_T183_P0.6790.77
267_L303_T0.6780.77
15_I71_L0.6780.77
21_V75_K0.6780.77
231_L302_L0.6770.77
157_T165_L0.6770.77
239_L259_V0.6760.77
63_A66_Q0.6730.76
242_R266_W0.6730.76
251_T256_E0.6690.76
45_L60_I0.6690.76
261_G267_L0.6680.76
146_P153_A0.6670.76
231_L269_V0.6660.76
200_Q261_G0.6640.75
280_L283_V0.6630.75
173_V177_T0.6630.75
118_S122_E0.6630.75
218_I222_N0.6630.75
199_D204_P0.6620.75
34_K39_T0.6610.75
197_D251_T0.6610.75
49_P55_A0.6600.75
36_G54_P0.6600.75
35_K39_T0.6570.75
200_Q203_T0.6560.75
11_A27_V0.6560.75
22_A29_V0.6550.74
9_A16_S0.6550.74
105_G111_R0.6520.74
136_D145_I0.6520.74
128_A181_Q0.6520.74
201_T220_F0.6510.74
215_D222_N0.6490.74
171_S178_M0.6490.74
76_A81_A0.6480.74
315_F322_F0.6460.73
118_S121_G0.6450.73
255_A295_F0.6450.73
201_T304_M0.6440.73
175_I282_N0.6380.73
229_D232_R0.6370.72
38_L41_Q0.6360.72
22_A25_D0.6350.72
174_Q210_E0.6350.72
154_D168_T0.6310.72
135_D164_R0.6310.72
178_M214_V0.6300.72
304_M308_G0.6270.71
305_L312_L0.6250.71
197_D254_S0.6210.71
145_I148_H0.6200.70
224_K272_C0.6140.70
148_H267_L0.6120.69
198_Y312_L0.6100.69
41_Q63_A0.6080.69
9_A40_L0.6080.69
109_V300_E0.6080.69
259_V269_V0.6070.69
27_V30_E0.6060.69
198_Y316_F0.6060.69
241_I259_V0.6060.69
312_L317_E0.6050.69
121_G127_V0.6050.69
246_S257_V0.6040.68
141_D155_H0.6040.68
137_Y283_V0.6030.68
242_R258_D0.6030.68
274_M280_L0.6020.68
42_M61_N0.6020.68
102_E324_K0.6020.68
15_I67_V0.6020.68
186_R215_D0.6010.68
44_T49_P0.6000.68
43_T60_I0.5960.67
216_T287_P0.5940.67
11_A14_A0.5930.67
185_I212_L0.5930.67
16_S70_A0.5930.67
42_M56_A0.5930.67
45_L48_L0.5880.66
209_M299_M0.5870.66
207_H297_M0.5870.66
119_F229_D0.5830.66
16_S19_S0.5810.65
15_I24_L0.5810.65
183_P285_I0.5770.65
246_S251_T0.5750.65
111_R115_R0.5690.64
32_L68_Q0.5680.64
120_F230_F0.5670.64
243_F255_A0.5660.63
59_V63_A0.5640.63
159_W162_T0.5640.63
202_H312_L0.5630.63
36_G61_N0.5630.63
235_F250_F0.5630.63
134_E139_N0.5630.63
155_H246_S0.5630.63
204_P317_E0.5620.63
113_I207_H0.5610.63
173_V272_C0.5600.63
246_S264_G0.5600.63
277_P286_D0.5590.62
220_F267_L0.5590.62
185_I285_I0.5590.62
314_S322_F0.5580.62
224_K295_F0.5560.62
141_D154_D0.5540.62
276_H280_L0.5540.62
47_E56_A0.5490.61
159_W197_D0.5490.61
16_S37_H0.5490.61
78_L83_L0.5480.61
55_A58_A0.5470.61
12_K65_E0.5470.61
109_V203_T0.5470.61
227_L262_K0.5460.61
271_G298_G0.5440.60
133_I160_F0.5420.60
156_D249_P0.5420.60
33_G39_T0.5410.60
121_G186_R0.5400.60
228_H240_Q0.5400.60
29_V39_T0.5400.60
196_N204_P0.5400.60
174_Q250_F0.5400.60
267_L305_L0.5360.59
42_M53_R0.5350.59
29_V35_K0.5350.59
32_L61_N0.5330.59
135_D160_F0.5330.59
264_G305_L0.5320.59
17_Q77_E0.5320.59
16_S23_A0.5290.58
128_A180_A0.5290.58
171_S275_V0.5280.58
51_E54_P0.5280.58
199_D203_T0.5270.58
220_F323_L0.5260.58
160_F164_R0.5260.58
318_N323_L0.5250.58
109_V315_F0.5240.58
242_R267_L0.5230.57
186_R243_F0.5230.57
204_P207_H0.5220.57
19_S102_E0.5220.57
113_I169_Q0.5210.57
49_P56_A0.5210.57
185_I213_I0.5200.57
78_L89_A0.5200.57
13_A89_A0.5200.57
48_L53_R0.5190.57
22_A40_L0.5170.57
187_I275_V0.5160.56
49_P54_P0.5150.56
108_P243_F0.5140.56
200_Q265_K0.5140.56
318_N322_F0.5140.56
117_E121_G0.5130.56
196_N200_Q0.5120.56
16_S74_R0.5110.56
204_P312_L0.5100.56
13_A22_A0.5100.56
32_L67_V0.5080.55
9_A26_N0.5080.55
154_D196_N0.5080.55
220_F247_Y0.5060.55
41_Q45_L0.5060.55
10_S19_S0.5060.55
115_R233_N0.5050.55
174_Q292_G0.5050.55
115_R119_F0.5050.55
31_Y37_H0.5040.55
169_Q269_V0.5040.55
79_E89_A0.5040.55
13_A84_N0.5030.55
231_L241_I0.5020.54
119_F123_L0.5020.54
190_P210_E0.5010.54
10_S16_S0.5000.54
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4p72C 2 0.7309 100 0.317 Contact Map
1b7yA 2 0.7217 100 0.326 Contact Map
3pcoA 2 0.9878 100 0.345 Contact Map
3l4gA 2 0.9878 100 0.49 Contact Map
3cmqA 1 0.7737 100 0.52 Contact Map
2rhqA 2 0.7615 100 0.567 Contact Map
2du3A 4 0.7829 100 0.586 Contact Map
2du7A 7 0.7706 100 0.605 Contact Map
2odrC 4 0.7584 100 0.616 Contact Map
2odrA 4 0.7584 100 0.624 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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