GREMLIN Database
RF2 - Peptide chain release factor 2
UniProt: P07012 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10762
Length: 365 (335)
Sequences: 5943 (3102)
Seq/√Len: 169.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
299_E302_K4.4141.00
297_E301_Q3.1911.00
301_Q305_A3.0601.00
130_L181_I3.0211.00
33_R59_E2.9961.00
159_R354_D2.9801.00
328_L349_L2.7361.00
31_K71_D2.7291.00
298_L302_K2.7241.00
244_Y273_Q2.7231.00
78_E82_G2.5771.00
156_A183_I2.5721.00
241_I263_I2.5271.00
49_E52_R2.4891.00
127_D225_E2.4881.00
245_R258_E2.4801.00
153_L163_T2.4801.00
239_L263_I2.4571.00
344_N347_A2.4051.00
239_L288_M2.3621.00
149_E181_I2.3481.00
72_Q109_K2.3091.00
37_V56_L2.2601.00
263_I288_M2.1801.00
240_R266_I2.1621.00
302_K306_E2.1591.00
281_H285_D2.1241.00
149_E179_V2.0971.00
159_R191_W2.0881.00
45_D48_N2.0811.00
348_V356_F2.0621.00
36_E40_E2.0311.00
246_T253_H1.9991.00
249_A253_H1.9961.00
136_S217_F1.9931.00
52_R55_A1.9881.00
79_D82_G1.9541.00
69_T116_R1.9431.00
64_E68_D1.9101.00
347_A352_S1.9051.00
112_Q116_R1.8961.00
164_E184_S1.8921.00
300_M304_N1.8641.00
40_E52_R1.8501.00
80_V103_L1.8151.00
132_I145_A1.8111.00
249_A278_R1.8111.00
208_D212_R1.7761.00
148_L218_S1.7531.00
253_H257_T1.7511.00
149_E153_L1.7471.00
75_Q79_D1.7321.00
109_K112_Q1.7151.00
253_H278_R1.7061.00
155_W354_D1.6871.00
296_Y300_M1.6791.00
105_A109_K1.6581.00
242_D264_T1.6411.00
253_H259_S1.6351.00
304_N308_Q1.6351.00
84_L107_E1.6271.00
340_V355_Q1.6261.00
38_N64_E1.6181.00
164_E182_K1.6111.00
300_M303_K1.6061.00
40_E43_Q1.5891.00
277_D282_K1.5811.00
127_D184_S1.5781.00
208_D214_H1.5691.00
41_L57_G1.5681.00
163_T181_I1.5671.00
60_R64_E1.5661.00
35_E38_N1.5381.00
253_H256_R1.5371.00
149_E165_I1.5181.00
38_N60_R1.4871.00
168_E176_I1.4871.00
241_I284_K1.4841.00
277_D286_Q1.4821.00
264_T271_V1.4681.00
333_I356_F1.4601.00
328_L345_T1.4461.00
229_D292_K1.4431.00
299_E303_K1.4231.00
246_T256_R1.4191.00
150_R350_D1.3941.00
276_N286_Q1.3911.00
70_L74_K1.3871.00
76_G109_K1.3771.00
243_V258_E1.3671.00
285_D289_K1.3661.00
162_K184_S1.3621.00
243_V261_V1.3581.00
303_K307_K1.3451.00
290_Q294_K1.3361.00
101_A105_A1.3311.00
51_E54_Q1.3301.00
51_E55_A1.3201.00
240_R264_T1.3191.00
306_E309_A1.3161.00
286_Q289_K1.3001.00
158_S352_S1.2941.00
108_E112_Q1.2911.00
132_I179_V1.2791.00
165_I179_V1.2761.00
156_A163_T1.2571.00
345_T349_L1.2471.00
97_N101_A1.2451.00
161_F192_L1.2441.00
281_H284_K1.2401.00
331_S346_Q1.2311.00
239_L266_I1.2281.00
342_T348_V1.2241.00
68_D72_Q1.2191.00
53_A57_G1.2171.00
28_D32_E1.2161.00
230_I296_Y1.2161.00
315_S321_S1.2141.00
301_Q304_N1.2011.00
33_R36_E1.1921.00
270_I298_L1.1911.00
41_L46_V1.1911.00
34_L60_R1.1791.00
139_T171_G1.1681.00
204_K211_G1.1571.00
325_S336_L1.1541.00
348_V353_L1.1511.00
76_G102_E1.1481.00
125_S187_Y1.1461.00
132_I220_A1.1431.00
182_K225_E1.1371.00
69_T72_Q1.1361.00
217_F316_D1.1281.00
260_A275_Q1.1261.00
98_E102_E1.1171.00
154_R350_D1.1101.00
153_L181_I1.1001.00
43_Q46_V1.0971.00
282_K289_K1.0971.00
333_I348_V1.0951.00
76_G110_L1.0911.00
304_N307_K1.0901.00
129_Y182_K1.0861.00
265_H269_G1.0751.00
191_W358_E1.0671.00
342_T355_Q1.0671.00
208_D211_G1.0671.00
129_Y166_I1.0651.00
183_I192_L1.0580.99
100_V104_D1.0540.99
305_A309_A1.0520.99
107_E111_A1.0480.99
253_H258_E1.0460.99
236_P239_L1.0430.99
72_Q76_G1.0380.99
50_P53_A1.0350.99
244_Y262_R1.0310.99
344_N355_Q1.0290.99
123_Y126_A1.0260.99
191_W354_D1.0230.99
128_C190_G1.0200.99
96_F100_V1.0190.99
243_V253_H1.0170.99
82_G85_E1.0170.99
158_S350_D1.0160.99
176_I179_V1.0150.99
308_Q312_D1.0100.99
97_N100_V1.0070.99
143_D173_V1.0070.99
73_M77_L1.0060.99
35_E39_A1.0050.99
340_V359_A1.0030.99
71_D74_K1.0000.99
130_L183_I0.9950.99
273_Q276_N0.9890.99
342_T345_T0.9860.99
95_T99_A0.9850.99
355_Q359_A0.9840.99
302_K305_A0.9810.99
303_K306_E0.9810.99
74_K78_E0.9760.99
147_M328_L0.9740.99
265_H268_T0.9700.99
50_P54_Q0.9640.99
234_I288_M0.9630.99
155_W191_W0.9580.99
101_A104_D0.9550.99
196_T221_F0.9490.99
33_R40_E0.9490.99
145_A218_S0.9490.99
68_D71_D0.9420.99
221_F311_E0.9420.99
125_S186_D0.9390.99
194_T360_S0.9360.99
67_V71_D0.9270.99
242_D262_R0.9260.99
47_W53_A0.9240.99
142_Q173_V0.9230.99
123_Y300_M0.9200.99
329_D332_R0.9190.99
297_E300_M0.9180.99
311_E314_K0.9180.99
270_I295_L0.9120.98
79_D106_L0.9120.98
31_K74_K0.9120.98
31_K35_E0.9110.98
149_E176_I0.9070.98
161_F188_A0.9070.98
81_S85_E0.9070.98
253_H281_H0.8990.98
276_N290_Q0.8950.98
353_L357_I0.8940.98
62_S186_D0.8940.98
276_N282_K0.8940.98
165_I168_E0.8930.98
32_E36_E0.8880.98
65_A68_D0.8850.98
47_W50_P0.8840.98
29_A32_E0.8790.98
127_D182_K0.8770.98
34_L67_V0.8750.98
239_L267_P0.8740.98
191_W361_L0.8730.98
34_L64_E0.8670.98
266_I269_G0.8660.98
331_S345_T0.8610.98
354_D358_E0.8560.98
82_G86_L0.8480.98
154_R157_E0.8470.98
236_P288_M0.8440.97
130_L192_L0.8440.97
273_Q290_Q0.8420.97
352_S355_Q0.8330.97
202_V325_S0.8320.97
256_R261_V0.8320.97
104_D107_E0.8300.97
284_K288_M0.8280.97
272_T295_L0.8250.97
39_A42_E0.8240.97
166_I182_K0.8230.97
146_S171_G0.8230.97
325_S332_R0.8190.97
94_E98_E0.8170.97
353_L356_F0.8170.97
155_W161_F0.8150.97
263_I284_K0.8130.97
28_D74_K0.8110.97
134_A141_A0.8100.97
292_K296_Y0.8040.97
40_E56_L0.8020.97
243_V249_A0.8020.97
202_V327_V0.8010.97
327_V332_R0.8000.97
126_A227_D0.7970.96
34_L63_L0.7970.96
226_V231_D0.7960.96
104_D108_E0.7960.96
282_K285_D0.7930.96
146_S176_I0.7890.96
278_R289_K0.7890.96
298_L301_Q0.7880.96
80_V106_L0.7870.96
103_L107_E0.7870.96
41_L60_R0.7860.96
26_D29_A0.7830.96
34_L38_N0.7800.96
182_K289_K0.7790.96
168_E179_V0.7770.96
340_V348_V0.7760.96
28_D31_K0.7740.96
204_K329_D0.7740.96
134_A169_S0.7720.96
230_I292_K0.7710.96
123_Y296_Y0.7710.96
231_D292_K0.7700.96
334_K341_E0.7640.95
204_K210_G0.7630.95
111_A114_E0.7620.95
357_I361_L0.7570.95
77_L81_S0.7560.95
295_L298_L0.7550.95
124_D193_R0.7540.95
94_E97_N0.7440.95
73_M76_G0.7410.95
244_Y275_Q0.7400.95
76_G106_L0.7390.95
142_Q146_S0.7380.95
249_A256_R0.7370.95
338_T359_A0.7360.94
242_D266_I0.7360.94
198_V313_N0.7300.94
26_D30_K0.7300.94
43_Q48_N0.7290.94
257_T279_S0.7260.94
241_I261_V0.7260.94
87_A99_A0.7250.94
106_L110_L0.7220.94
338_T360_S0.7220.94
254_V258_E0.7210.94
241_I288_M0.7210.94
80_V110_L0.7180.94
155_W159_R0.7160.94
82_G89_E0.7160.94
346_Q350_D0.7160.94
86_L89_E0.7150.94
219_S317_I0.7150.94
232_I292_K0.7150.94
32_E35_E0.7150.94
72_Q75_Q0.7120.93
166_I290_Q0.7110.93
247_S257_T0.7110.93
158_S354_D0.7110.93
55_A59_E0.7100.93
136_S318_G0.7090.93
38_N42_E0.7050.93
330_D334_K0.7040.93
105_A108_E0.7040.93
71_D75_Q0.7020.93
76_G113_L0.7020.93
76_G80_V0.7010.93
256_R281_H0.6980.93
264_T273_Q0.6980.93
134_A142_Q0.6970.93
336_L339_G0.6970.93
91_D99_A0.6970.93
240_R273_Q0.6950.93
46_V56_L0.6950.93
153_L157_E0.6930.93
358_E362_K0.6930.93
93_E97_N0.6930.93
83_L87_A0.6900.92
274_C286_Q0.6900.92
149_E163_T0.6900.92
80_V84_L0.6880.92
238_D267_P0.6880.92
89_E92_D0.6880.92
147_M349_L0.6840.92
245_R253_H0.6820.92
153_L179_V0.6810.92
126_A226_V0.6800.92
59_E62_S0.6790.92
84_L88_V0.6790.92
306_E310_M0.6780.92
227_D230_I0.6780.92
83_L99_A0.6780.92
98_E101_A0.6770.92
234_I263_I0.6760.92
65_A116_R0.6750.92
95_T98_E0.6750.92
57_G60_R0.6750.92
333_I345_T0.6740.91
338_T356_F0.6690.91
288_M292_K0.6680.91
172_E322_Q0.6660.91
256_R273_Q0.6600.91
78_E81_S0.6600.91
46_V52_R0.6590.91
151_M353_L0.6590.91
217_F318_G0.6500.90
263_I272_T0.6490.90
244_Y264_T0.6480.90
46_V53_A0.6450.90
149_E168_E0.6450.90
226_V293_A0.6430.89
232_I295_L0.6430.89
268_T295_L0.6400.89
167_E180_T0.6380.89
116_R187_Y0.6380.89
310_M313_N0.6350.89
108_E111_A0.6340.89
111_A115_F0.6330.89
274_C290_Q0.6330.89
65_A69_T0.6310.89
338_T341_E0.6290.88
87_A95_T0.6280.88
132_I149_E0.6270.88
142_Q177_K0.6270.88
356_F359_A0.6260.88
63_L67_V0.6250.88
36_E39_A0.6220.88
190_G361_L0.6220.88
31_K67_V0.6220.88
332_R336_L0.6220.88
56_L60_R0.6210.88
85_E89_E0.6190.88
266_I270_I0.6170.87
128_C189_Y0.6150.87
87_A103_L0.6130.87
272_T294_K0.6130.87
244_Y260_A0.6130.87
240_R288_M0.6110.87
272_T290_Q0.6110.87
155_W357_I0.6110.87
80_V102_E0.6100.87
331_S341_E0.6100.87
246_T260_A0.6090.87
77_L110_L0.6070.87
229_D232_I0.6060.87
342_T356_F0.6060.87
343_R347_A0.6030.86
235_N238_D0.6030.86
143_D146_S0.6020.86
92_D95_T0.6000.86
184_S188_A0.5970.86
62_S120_S0.5970.86
140_E214_H0.5970.86
61_S64_E0.5960.86
330_D341_E0.5920.85
307_K311_E0.5920.85
198_V217_F0.5920.85
146_S168_E0.5910.85
249_A258_E0.5910.85
286_Q290_Q0.5890.85
150_R154_R0.5880.85
80_V107_E0.5870.85
119_F193_R0.5870.85
41_L53_A0.5860.85
30_K66_V0.5850.85
309_A312_D0.5850.85
143_D328_L0.5850.85
169_S178_S0.5840.84
133_Q219_S0.5840.84
83_L95_T0.5830.84
84_L103_L0.5830.84
256_R282_K0.5820.84
139_T173_V0.5810.84
210_G214_H0.5810.84
282_K286_Q0.5800.84
226_V296_Y0.5800.84
188_A192_L0.5780.84
32_E43_Q0.5770.84
154_R158_S0.5760.84
132_I218_S0.5760.84
209_S214_H0.5750.84
246_T273_Q0.5720.83
39_A43_Q0.5710.83
347_A350_D0.5700.83
55_A58_K0.5690.83
141_A198_V0.5670.83
79_D83_L0.5650.83
244_Y271_V0.5640.82
146_S173_V0.5600.82
141_A214_H0.5570.82
147_M206_P0.5560.82
52_R56_L0.5550.81
167_E178_S0.5550.81
253_H279_S0.5540.81
134_A168_E0.5530.81
83_L102_E0.5500.81
83_L86_L0.5500.81
66_V116_R0.5480.81
150_R153_L0.5480.81
87_A96_F0.5450.80
82_G98_E0.5440.80
161_F191_W0.5430.80
230_I300_M0.5420.80
141_A218_S0.5410.80
272_T277_D0.5410.80
198_V219_S0.5400.80
135_G217_F0.5390.79
234_I292_K0.5390.79
81_S84_L0.5390.79
226_V300_M0.5370.79
243_V281_H0.5360.79
62_S125_S0.5350.79
53_A60_R0.5320.79
223_Y293_A0.5320.79
131_D212_R0.5320.79
31_K34_L0.5310.78
295_L299_E0.5300.78
30_K63_L0.5290.78
217_F321_S0.5290.78
333_I342_T0.5290.78
147_M201_L0.5280.78
69_T113_L0.5260.78
135_G198_V0.5250.78
134_A177_K0.5230.77
331_S336_L0.5220.77
243_V256_R0.5210.77
241_I264_T0.5210.77
258_E281_H0.5210.77
247_S273_Q0.5210.77
107_E110_L0.5210.77
48_N53_A0.5190.77
249_A284_K0.5180.77
261_V272_T0.5170.77
238_D266_I0.5160.77
221_F294_K0.5160.77
240_R257_T0.5130.76
139_T172_E0.5120.76
54_Q57_G0.5100.76
124_D190_G0.5100.76
198_V321_S0.5080.75
253_H276_N0.5070.75
194_T357_I0.5070.75
85_E88_V0.5050.75
133_Q221_F0.5040.75
136_S141_A0.5030.75
147_M217_F0.5030.75
76_G79_D0.5020.75
247_S258_E0.5010.74
198_V218_S0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zbtA 1 0.8438 100 0.127 Contact Map
1gqeA 1 0.9918 100 0.128 Contact Map
2ihr1 1 0.9534 100 0.132 Contact Map
3d5aX 1 0.9288 100 0.139 Contact Map
2b3tB 1 0.7973 100 0.142 Contact Map
1rq0A 3 0.8411 100 0.191 Contact Map
2rsmA 1 0.3151 100 0.705 Contact Map
3j7yp 1 0.1589 100 0.744 Contact Map
1j26A 1 0.2384 100 0.793 Contact Map
4dh9Y 1 0.2904 99.9 0.805 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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