GREMLIN Database
PFKB - ATP-dependent 6-phosphofructokinase isozyme 2
UniProt: P06999 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10700
Length: 309 (280)
Sequences: 22558 (16871)
Seq/√Len: 1008.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
53_G264_L3.1551.00
135_V164_I2.6161.00
70_V85_A2.5481.00
56_T79_P2.5201.00
225_L231_L2.4691.00
232_G276_V2.4461.00
265_K270_A2.3011.00
271_S274_E2.2691.00
63_G87_D2.2631.00
230_A258_M2.2211.00
154_S180_N2.1171.00
213_V233_V2.0621.00
67_E85_A2.0421.00
6_T131_G2.0221.00
48_I53_G2.0071.00
60_P86_K2.0021.00
239_I273_E1.9781.00
188_Q225_L1.9771.00
156_A161_I1.9531.00
164_I263_T1.9241.00
265_K278_F1.8871.00
50_H76_E1.8761.00
133_I162_R1.8431.00
296_S299_D1.8341.00
206_R238_C1.8211.00
209_A233_V1.8121.00
153_I163_C1.8101.00
232_G239_I1.7911.00
257_S282_A1.7891.00
185_K259_V1.7791.00
74_A80_V1.7651.00
46_R76_E1.7641.00
162_R182_E1.7621.00
270_A274_E1.7451.00
47_A260_G1.7361.00
182_E219_K1.7141.00
231_L240_Q1.7101.00
268_E297_H1.7041.00
7_L45_A1.6971.00
230_A280_V1.6951.00
72_L75_D1.6861.00
254_A287_T1.6641.00
273_E277_R1.6241.00
71_S75_D1.6131.00
262_M275_M1.6061.00
283_G287_T1.6061.00
70_V74_A1.5941.00
60_P120_Q1.5921.00
225_L240_Q1.5841.00
211_E215_S1.5831.00
183_L220_R1.5811.00
289_N299_D1.5741.00
222_V262_M1.5721.00
133_I164_I1.5711.00
11_P59_F1.5701.00
261_A282_A1.5601.00
220_R234_D1.5461.00
244_P281_A1.5361.00
211_E214_N1.5241.00
151_Q154_S1.5061.00
155_A159_Q1.4901.00
234_D237_N1.4901.00
48_I264_L1.4881.00
150_T154_S1.4671.00
225_L228_Q1.4661.00
63_G67_E1.4621.00
232_G272_L1.4551.00
35_F68_H1.4391.00
250_S291_G1.4391.00
241_V276_V1.4291.00
151_Q155_A1.4181.00
133_I263_T1.4171.00
150_T174_A1.4061.00
261_A278_F1.4021.00
155_A158_K1.3991.00
37_P69_L1.3851.00
181_I184_V1.3731.00
162_R183_L1.3731.00
67_E71_S1.3621.00
129_E159_Q1.3571.00
166_D224_S1.3571.00
177_A180_N1.3481.00
42_I73_L1.3371.00
241_V280_V1.3331.00
210_Q214_N1.3291.00
63_G85_A1.3291.00
157_Q182_E1.3131.00
167_S186_P1.3071.00
204_D207_K1.2971.00
244_P277_R1.2911.00
117_E120_Q1.2881.00
54_S79_P1.2831.00
213_V235_S1.2771.00
157_Q180_N1.2691.00
264_L268_E1.2641.00
13_L68_H1.2621.00
206_R210_Q1.2611.00
246_V288_L1.2411.00
177_A215_S1.2371.00
230_A276_V1.2341.00
118_F151_Q1.2341.00
72_L76_E1.2331.00
220_R262_M1.2291.00
37_P72_L1.2281.00
233_V238_C1.2211.00
70_V83_V1.2201.00
162_R219_K1.2161.00
261_A279_G1.1991.00
173_S215_S1.1991.00
225_L229_G1.1941.00
122_E151_Q1.1901.00
185_K224_S1.1881.00
15_S33_P1.1821.00
220_R272_L1.1811.00
210_Q233_V1.1781.00
265_K275_M1.1691.00
220_R266_L1.1671.00
262_M266_L1.1631.00
18_I32_A1.1621.00
262_M279_G1.1611.00
244_P280_V1.1551.00
134_L156_A1.1511.00
177_A181_I1.1491.00
210_Q238_C1.1471.00
20_P31_T1.1461.00
224_S258_M1.1441.00
248_S287_T1.1431.00
67_E70_V1.1351.00
53_G267_A1.1351.00
45_A55_A1.1311.00
246_V287_T1.1201.00
68_H71_S1.1181.00
191_L223_V1.1141.00
274_E277_R1.1101.00
51_L261_A1.1081.00
49_A78_V1.0991.00
48_I55_A1.0971.00
44_V135_V1.0961.00
154_S158_K1.0861.00
71_S74_A1.0861.00
285_A289_N1.0771.00
265_K268_E1.0751.00
183_L262_M1.0751.00
136_I163_C1.0661.00
285_A288_L1.0621.00
143_G147_E1.0611.00
220_R232_G1.0611.00
209_A223_V1.0591.00
207_K210_Q1.0541.00
248_S251_T1.0491.00
183_L266_L1.0471.00
244_P284_S1.0391.00
89_T115_E1.0371.00
49_A77_N1.0371.00
135_V263_T1.0371.00
46_R50_H1.0341.00
56_T131_G1.0211.00
191_L208_A1.0191.00
56_T82_T1.0171.00
53_G268_E1.0121.00
188_Q228_Q1.0121.00
134_L161_I1.0051.00
30_C106_F1.0051.00
15_S35_F1.0021.00
280_V283_G0.9881.00
262_M272_L0.9861.00
48_I260_G0.9851.00
185_K262_M0.9851.00
172_L177_A0.9841.00
171_A174_A0.9831.00
47_A257_S0.9771.00
243_P280_V0.9731.00
135_V259_V0.9681.00
68_H72_L0.9671.00
7_L41_G0.9631.00
37_P68_H0.9611.00
165_V181_I0.9611.00
257_S286_A0.9571.00
147_E151_Q0.9561.00
61_A70_V0.9541.00
219_K235_S0.9501.00
61_A66_G0.9501.00
277_R281_A0.9471.00
174_A177_A0.9411.00
91_Q96_H0.9391.00
92_N96_H0.9371.00
131_G161_I0.9341.00
51_L282_A0.9301.00
195_V208_A0.9291.00
275_M279_G0.9271.00
86_K89_T0.9201.00
171_A175_A0.9201.00
20_P99_A0.9181.00
228_Q240_Q0.9161.00
49_A54_S0.9121.00
187_N190_E0.9121.00
264_L278_F0.9031.00
84_E120_Q0.9031.00
86_K115_E0.9031.00
128_I131_G0.9011.00
136_I153_I0.8991.00
264_L297_H0.8981.00
248_S288_L0.8941.00
188_Q192_S0.8901.00
80_V83_V0.8881.00
166_D185_K0.8871.00
171_A177_A0.8841.00
11_P69_L0.8781.00
70_V80_V0.8731.00
18_I34_V0.8721.00
229_G243_P0.8711.00
61_A83_V0.8691.00
118_F147_E0.8681.00
237_N273_E0.8681.00
247_K288_L0.8641.00
118_F121_L0.8601.00
266_L275_M0.8601.00
61_A85_A0.8531.00
257_S294_L0.8511.00
103_Q107_V0.8491.00
231_L238_C0.8441.00
60_P84_E0.8421.00
42_I293_R0.8401.00
241_V277_R0.8401.00
16_A36_E0.8381.00
44_V259_V0.8361.00
166_D186_P0.8351.00
173_S177_A0.8341.00
15_S65_T0.8321.00
241_V273_E0.8291.00
270_A278_F0.8191.00
239_I276_V0.8181.00
261_A265_K0.8161.00
258_M280_V0.8131.00
97_V100_S0.8131.00
81_A124_Q0.8121.00
210_Q213_V0.8101.00
249_Q290_Q0.8071.00
271_S275_M0.8071.00
6_T58_I0.8061.00
11_P42_I0.8021.00
172_L175_A0.8021.00
136_I149_L0.8011.00
186_P191_L0.7991.00
119_R122_E0.7991.00
257_S261_A0.7961.00
118_F122_E0.7941.00
246_V284_S0.7901.00
8_T152_L0.7841.00
24_P102_E0.7841.00
49_A55_A0.7841.00
11_P57_A0.7791.00
209_A221_V0.7791.00
98_E102_E0.7791.00
21_Q26_G0.7751.00
91_Q94_H0.7721.00
93_L96_H0.7661.00
146_L150_T0.7641.00
128_I161_I0.7621.00
164_I185_K0.7531.00
8_T134_L0.7521.00
153_I180_N0.7521.00
133_I267_A0.7521.00
90_R93_L0.7501.00
45_A57_A0.7501.00
234_D272_L0.7491.00
56_T81_A0.7471.00
118_F148_K0.7451.00
257_S283_G0.7441.00
207_K211_E0.7421.00
176_L181_I0.7361.00
222_V232_G0.7331.00
209_A238_C0.7291.00
213_V234_D0.7251.00
72_L293_R0.7241.00
91_Q95_V0.7221.00
35_F65_T0.7221.00
234_D239_I0.7221.00
165_V175_A0.7211.00
278_F297_H0.7191.00
206_R240_Q0.7161.00
283_G286_A0.7151.00
146_L171_A0.7141.00
206_R233_V0.7111.00
164_I183_L0.7101.00
150_T180_N0.7091.00
70_V84_E0.7031.00
280_V284_S0.7031.00
46_R293_R0.7011.00
18_I290_Q0.6991.00
278_F300_T0.6921.00
202_P207_K0.6901.00
102_E105_R0.6901.00
281_A285_A0.6881.00
89_T92_N0.6841.00
213_V216_G0.6831.00
253_G294_L0.6811.00
51_L278_F0.6811.00
14_D38_G0.6791.00
191_L212_I0.6791.00
144_V147_E0.6761.00
209_A213_V0.6691.00
34_V290_Q0.6681.00
6_T56_T0.6671.00
90_R94_H0.6581.00
270_A275_M0.6581.00
205_V225_L0.6571.00
258_M279_G0.6561.00
64_A91_Q0.6541.00
258_M283_G0.6541.00
206_R231_L0.6521.00
51_L264_L0.6501.00
51_L300_T0.6481.00
104_Y107_V0.6481.00
227_P245_P0.6471.00
124_Q127_E0.6471.00
148_K152_L0.6461.00
202_P205_V0.6421.00
40_G168_S0.6391.00
45_A78_V0.6381.00
258_M276_V0.6371.00
164_I259_V0.6351.00
147_E150_T0.6351.00
54_S267_A0.6341.00
17_T33_P0.6341.00
42_I57_A0.6321.00
230_A241_V0.6321.00
285_A299_D0.6301.00
229_G240_Q0.6291.00
15_S93_L0.6281.00
282_A300_T0.6241.00
13_L65_T0.6211.00
122_E155_A0.6171.00
202_P240_Q0.6101.00
102_E108_M0.6101.00
276_V280_V0.6091.00
191_L205_V0.6091.00
54_S132_A0.6081.00
63_G88_W0.6081.00
184_V221_V0.6081.00
150_T171_A0.6061.00
213_V221_V0.6061.00
134_L152_L0.6061.00
119_R123_E0.6041.00
38_G43_N0.6031.00
163_C181_I0.6021.00
222_V258_M0.5981.00
143_G146_L0.5981.00
150_T153_I0.5951.00
104_Y108_M0.5931.00
213_V236_E0.5911.00
269_N275_M0.5891.00
96_H105_R0.5881.00
13_L35_F0.5861.00
133_I183_L0.5861.00
59_F83_V0.5861.00
156_A163_C0.5861.00
120_Q123_E0.5831.00
185_K222_V0.5821.00
166_D256_D0.5791.00
218_A221_V0.5791.00
44_V263_T0.5771.00
37_P293_R0.5741.00
274_E278_F0.5741.00
260_G264_L0.5741.00
285_A300_T0.5721.00
17_T95_V0.5711.00
67_E74_A0.5711.00
216_G219_K0.5701.00
142_P165_V0.5681.00
76_E296_S0.5681.00
98_E101_G0.5661.00
51_L260_G0.5661.00
205_V208_A0.5641.00
150_T175_A0.5621.00
165_V172_L0.5581.00
97_V102_E0.5551.00
48_I263_T0.5551.00
116_D120_Q0.5551.00
133_I266_L0.5541.00
295_C300_T0.5521.00
17_T30_C0.5511.00
92_N95_V0.5511.00
189_K193_A0.5491.00
49_A76_E0.5481.00
182_E220_R0.5471.00
114_N117_E0.5461.00
98_E104_Y0.5451.00
63_G86_K0.5451.00
73_L78_V0.5441.00
132_A162_R0.5411.00
272_L276_V0.5351.00
227_P248_S0.5341.00
216_G221_V0.5331.00
12_S112_A0.5321.00
82_T85_A0.5311.00
226_G251_T0.5311.00
213_V218_A0.5311.00
241_V244_P0.5261.00
170_E193_A0.5251.00
132_A161_I0.5231.00
42_I46_R0.5231.00
188_Q226_G0.5231.00
156_A159_Q0.5211.00
106_F109_P0.5201.00
272_L275_M0.5201.00
252_V292_T0.5201.00
132_A267_A0.5191.00
265_K269_N0.5181.00
106_F110_G0.5181.00
27_K40_G0.5151.00
36_E293_R0.5141.00
224_S256_D0.5131.00
44_V260_G0.5131.00
84_E123_E0.5131.00
202_P211_E0.5121.00
173_S176_L0.5111.00
41_G45_A0.5081.00
81_A84_E0.5061.00
191_L233_V0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3umoA 4 0.9838 100 0.181 Contact Map
3vasA 1 0.9579 100 0.211 Contact Map
2jg1A 2 0.9773 100 0.213 Contact Map
1vm7A 2 0.9385 100 0.213 Contact Map
2rbcA 2 0.9482 100 0.214 Contact Map
2fv7A 2 0.9579 100 0.214 Contact Map
3ry7A 2 0.9515 100 0.214 Contact Map
2f02A 2 0.9709 100 0.214 Contact Map
2ajrA 2 0.9741 100 0.215 Contact Map
3ljsA 2 0.9417 100 0.219 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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