GREMLIN Database
HIS7 - Histidine biosynthesis bifunctional protein HisB
UniProt: P06987 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10445
Length: 355 (351)
Sequences: 474 (215)
Seq/√Len: 11.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
135_I145_G6.8161.00
77_M90_V6.4681.00
15_I24_V5.9121.00
14_L80_F5.7471.00
174_N179_Q5.1671.00
27_F72_P4.2501.00
27_F75_L4.0280.99
243_L305_L3.7950.99
266_L270_A3.7200.99
66_Q70_D3.4740.98
170_H183_Q3.4250.98
95_H101_C3.1920.97
198_V292_D3.1680.96
15_I76_M3.1610.96
111_V141_M2.8750.94
241_E245_I2.8470.93
89_E113_R2.8070.93
232_V293_L2.7470.92
192_S209_A2.7460.92
127_V137_L2.5220.88
172_V179_Q2.5090.88
244_K310_G2.4100.86
35_G154_N2.4070.86
237_L308_T2.3880.85
322_D325_R2.3380.84
265_C328_S2.2710.82
38_P41_L2.2390.81
30_L76_M2.2360.81
79_I82_S2.2340.81
192_S206_D2.2310.81
37_I79_I2.2100.80
193_K217_E2.1560.79
259_V270_A2.1550.78
210_T351_S2.0000.73
305_L311_V1.9980.73
20_S24_V1.9900.73
3_Q49_K1.9820.72
49_K88_D1.9770.72
170_H181_D1.9410.71
33_E148_Y1.8480.67
298_I326_V1.8480.67
171_V220_V1.8170.65
52_M90_V1.7990.64
319_G325_R1.7990.64
38_P83_Q1.7830.64
78_Q81_T1.7690.63
15_I20_S1.7410.62
72_P75_L1.7370.62
39_E156_P1.7200.61
209_A333_F1.7110.60
132_A147_R1.7000.60
315_L329_L1.6930.60
108_V111_V1.6820.59
237_L277_P1.6740.59
212_G341_I1.6580.58
289_R292_D1.6560.58
95_H103_C1.6560.58
199_G225_Y1.6500.57
64_F68_D1.6430.57
256_F334_G1.6410.57
315_L326_V1.6340.57
265_C317_T1.6320.57
101_C107_K1.6240.56
80_F265_C1.6120.56
93_C103_C1.5780.54
279_L299_E1.5740.54
35_G156_P1.5660.53
91_L111_V1.5610.53
27_F71_G1.5480.53
107_K136_Q1.5400.52
348_L354_V1.5380.52
94_P103_C1.4790.49
45_K159_G1.4770.49
4_K124_N1.4740.49
32_F79_I1.4610.48
264_E274_S1.4560.48
136_Q139_E1.4550.48
52_M64_F1.4450.47
48_Y166_D1.4300.47
5_Y49_K1.4120.46
28_D75_L1.4100.46
111_V137_L1.4090.46
43_L50_L1.4020.45
73_H90_V1.4000.45
14_L37_I1.3910.45
26_R30_L1.3610.43
36_V80_F1.3560.43
220_V235_T1.3540.43
44_Q50_L1.3520.43
287_Y296_E1.3400.42
293_L298_I1.3400.42
254_C274_S1.3310.42
220_V238_A1.3300.42
63_S96_L1.3290.42
192_S333_F1.3250.42
72_P76_M1.3160.41
91_L115_L1.3110.41
303_R307_Y1.3100.41
93_C101_C1.3040.41
176_K335_R1.3000.40
42_K159_G1.2950.40
335_R338_R1.2610.39
159_G163_T1.2600.39
73_H76_M1.2540.38
317_T328_S1.2510.38
15_I37_I1.2490.38
208_I333_F1.2320.37
267_A328_S1.2280.37
95_H110_L1.2130.36
225_Y289_R1.2030.36
81_T86_Q1.1970.36
73_H77_M1.1860.35
195_N219_N1.1800.35
48_Y158_I1.1790.35
108_V184_V1.1760.35
267_A311_V1.1750.35
226_I238_A1.1730.35
226_I274_S1.1710.35
15_I22_F1.1700.34
179_Q221_K1.1640.34
31_A82_S1.1530.34
45_K163_T1.1500.34
8_I194_I1.1490.34
94_P101_C1.1460.33
181_D217_E1.1420.33
62_Q67_A1.1380.33
314_H318_K1.1370.33
101_C110_L1.1060.32
292_D342_R1.1010.31
228_D294_S1.1000.31
91_L109_K1.0970.31
122_R141_M1.0940.31
208_I236_G1.0800.31
241_E297_M1.0730.30
52_M80_F1.0640.30
198_V209_A1.0640.30
188_R215_R1.0630.30
8_I128_I1.0630.30
26_R68_D1.0620.30
132_A135_I1.0610.30
31_A78_Q1.0610.30
141_M220_V1.0500.29
4_K48_Y1.0490.29
54_T139_E1.0450.29
190_G206_D1.0390.29
294_S299_E1.0370.29
22_F183_Q1.0360.29
54_T76_M1.0340.29
199_G292_D1.0280.28
15_I30_L1.0170.28
183_Q217_E1.0160.28
282_K318_K1.0150.28
206_D346_D1.0120.28
26_R75_L1.0070.28
285_F329_L0.9980.27
212_G274_S0.9970.27
37_I76_M0.9960.27
16_S31_A0.9950.27
281_Y298_I0.9900.27
42_K45_K0.9780.26
68_D72_P0.9770.26
73_H170_H0.9640.26
171_V238_A0.9630.26
226_I306_S0.9610.26
116_A119_A0.9600.26
48_Y62_Q0.9560.26
320_K325_R0.9460.25
209_A328_S0.9440.25
85_V141_M0.9400.25
135_I147_R0.9400.25
30_L72_P0.9370.25
21_D29_K0.9370.25
80_F143_I0.9350.25
218_I235_T0.9350.25
199_G249_D0.9340.25
277_P308_T0.9330.25
271_L337_L0.9280.25
14_L30_L0.9270.25
41_L44_Q0.9240.24
24_V72_P0.9200.24
36_V85_V0.9180.24
22_F97_P0.9130.24
288_Q294_S0.9110.24
180_I242_A0.9090.24
317_T329_L0.9080.24
168_Y183_Q0.9070.24
273_I309_M0.8990.24
199_G289_R0.8970.24
62_Q226_I0.8960.23
271_L336_T0.8890.23
282_K316_K0.8860.23
93_C312_T0.8790.23
66_Q71_G0.8790.23
135_I146_L0.8780.23
18_P21_D0.8780.23
24_V64_F0.8760.23
50_L152_T0.8750.23
127_V138_A0.8700.23
164_R343_V0.8690.23
124_N216_M0.8640.22
27_F64_F0.8620.22
75_L114_Y0.8570.22
328_S332_A0.8560.22
228_D273_I0.8540.22
4_K288_Q0.8530.22
89_E218_I0.8500.22
111_V122_R0.8490.22
136_Q226_I0.8460.22
185_W215_R0.8440.22
283_A329_L0.8430.22
141_M317_T0.8430.22
68_D71_G0.8410.22
31_A98_A0.8370.21
162_L275_G0.8350.21
180_I238_A0.8350.21
209_A241_E0.8330.21
126_Y146_L0.8320.21
89_E112_E0.8320.21
210_T256_F0.8310.21
120_M325_R0.8290.21
16_S146_L0.8250.21
25_D29_K0.8240.21
101_C136_Q0.8180.21
111_V120_M0.8170.21
269_C315_L0.8140.21
266_L316_K0.8060.20
226_I242_A0.8050.20
146_L153_L0.8030.20
37_I97_P0.8020.20
256_F332_A0.8020.20
148_Y351_S0.7990.20
171_V277_P0.7960.20
35_G39_E0.7930.20
4_K248_G0.7850.20
32_F311_V0.7840.20
271_L342_R0.7840.20
208_I271_L0.7820.20
171_V242_A0.7820.20
93_C273_I0.7820.20
193_K219_N0.7800.20
317_T325_R0.7740.19
208_I337_L0.7710.19
75_L79_I0.7680.19
159_G335_R0.7680.19
73_H92_I0.7650.19
39_E206_D0.7610.19
170_H217_E0.7600.19
93_C107_K0.7600.19
97_P110_L0.7580.19
251_R345_G0.7550.19
214_F337_L0.7520.19
169_A238_A0.7480.19
14_L38_P0.7460.19
259_V268_R0.7450.19
182_V231_T0.7430.19
266_L314_H0.7420.18
282_K314_H0.7420.18
253_I271_L0.7410.18
259_V266_L0.7390.18
146_L239_L0.7380.18
225_Y237_L0.7380.18
91_L110_L0.7370.18
232_V346_D0.7370.18
148_Y258_F0.7360.18
48_Y124_N0.7360.18
22_F27_F0.7360.18
91_L145_G0.7360.18
273_I312_T0.7300.18
254_C315_L0.7300.18
73_H158_I0.7300.18
48_Y81_T0.7270.18
110_L182_V0.7250.18
73_H347_T0.7240.18
108_V122_R0.7230.18
319_G322_D0.7220.18
80_F85_V0.7190.18
296_E325_R0.7180.18
110_L212_G0.7170.18
312_T334_G0.7160.18
37_I80_F0.7160.18
32_F325_R0.7150.18
94_P110_L0.7130.18
190_G215_R0.7120.18
280_E351_S0.7100.18
33_E149_D0.7080.17
46_A83_Q0.7060.17
97_P114_Y0.7060.17
35_G220_V0.7010.17
30_L73_H0.7000.17
184_V218_I0.6990.17
5_Y16_S0.6990.17
193_K354_V0.6980.17
141_M256_F0.6960.17
77_M163_T0.6950.17
95_H182_V0.6940.17
294_S312_T0.6930.17
120_M322_D0.6910.17
228_D343_V0.6910.17
16_S20_S0.6900.17
5_Y117_E0.6890.17
257_G336_T0.6880.17
24_V149_D0.6870.17
44_Q136_Q0.6850.17
30_L75_L0.6840.17
126_Y136_Q0.6830.17
266_L338_R0.6830.17
81_T341_I0.6820.17
43_L107_K0.6820.17
42_K100_E0.6810.17
309_M312_T0.6810.17
279_L308_T0.6800.17
264_E299_E0.6800.17
116_A317_T0.6780.17
115_L141_M0.6770.17
108_V112_E0.6770.17
237_L346_D0.6760.17
157_M160_E0.6750.17
160_E315_L0.6740.17
249_D348_L0.6730.17
213_G242_A0.6690.16
268_R314_H0.6690.16
48_Y344_E0.6660.16
290_V306_S0.6660.16
153_L212_G0.6640.16
42_K88_D0.6630.16
282_K320_K0.6620.16
195_N199_G0.6620.16
93_C294_S0.6590.16
29_K64_F0.6590.16
83_Q215_R0.6590.16
208_I265_C0.6580.16
280_E314_H0.6520.16
15_I123_A0.6500.16
249_D306_S0.6500.16
259_V271_L0.6500.16
277_P335_R0.6480.16
73_H340_A0.6480.16
150_R206_D0.6480.16
294_S334_G0.6460.16
290_V320_K0.6440.16
275_G303_R0.6440.16
133_T194_I0.6430.16
215_R228_D0.6410.16
75_L99_D0.6400.16
228_D287_Y0.6400.16
73_H79_I0.6370.16
100_E111_V0.6370.16
39_E159_G0.6360.16
312_T329_L0.6350.16
314_H337_L0.6340.15
139_E154_N0.6340.15
68_D237_L0.6320.15
144_T219_N0.6320.15
318_K325_R0.6320.15
5_Y135_I0.6310.15
209_A292_D0.6310.15
171_V226_I0.6300.15
311_V325_R0.6280.15
197_G202_D0.6280.15
161_Q333_F0.6270.15
180_I285_F0.6270.15
37_I41_L0.6260.15
169_A244_K0.6250.15
159_G164_R0.6250.15
160_E164_R0.6240.15
296_E322_D0.6220.15
312_T349_P0.6190.15
264_E325_R0.6170.15
186_L253_I0.6170.15
54_T94_P0.6160.15
83_Q188_R0.6150.15
50_L85_V0.6150.15
141_M271_L0.6140.15
52_M89_E0.6140.15
243_L311_V0.6130.15
91_L113_R0.6120.15
208_I259_V0.6120.15
148_Y308_T0.6120.15
13_T18_P0.6120.15
268_R318_K0.6120.15
32_F273_I0.6110.15
27_F297_M0.6090.15
192_S328_S0.6080.15
256_F351_S0.6080.15
46_A298_I0.6070.15
254_C264_E0.6060.15
210_T333_F0.6030.15
138_A145_G0.6030.15
170_H179_Q0.6000.15
64_F72_P0.5990.15
113_R124_N0.5980.15
159_G305_L0.5970.15
269_C329_L0.5950.15
317_T332_A0.5950.15
305_L335_R0.5940.14
3_Q235_T0.5930.14
213_G249_D0.5930.14
218_I239_L0.5920.14
149_D308_T0.5910.14
18_P30_L0.5910.14
124_N245_I0.5900.14
212_G345_G0.5890.14
30_L50_L0.5890.14
182_V226_I0.5880.14
53_I251_R0.5870.14
97_P107_K0.5860.14
198_V299_E0.5860.14
81_T236_G0.5850.14
148_Y210_T0.5840.14
169_A210_T0.5820.14
209_A216_M0.5820.14
33_E264_E0.5810.14
210_T329_L0.5810.14
22_F37_I0.5800.14
232_V326_V0.5800.14
256_F269_C0.5800.14
123_A174_N0.5790.14
62_Q192_S0.5790.14
24_V146_L0.5780.14
146_L161_Q0.5770.14
21_D63_S0.5770.14
93_C102_D0.5750.14
136_Q168_Y0.5750.14
305_L315_L0.5740.14
292_D320_K0.5740.14
100_E149_D0.5730.14
101_C148_Y0.5730.14
158_I162_L0.5730.14
62_Q145_G0.5730.14
220_V231_T0.5710.14
228_D334_G0.5680.14
271_L305_L0.5670.14
66_Q94_P0.5660.14
122_R309_M0.5660.14
265_C279_L0.5660.14
86_Q235_T0.5610.14
120_M281_Y0.5610.14
237_L282_K0.5600.14
110_L143_I0.5590.14
14_L99_D0.5570.14
216_M312_T0.5570.14
62_Q74_N0.5550.14
198_V224_L0.5540.14
89_E110_L0.5540.14
169_A184_V0.5540.14
278_H322_D0.5530.14
225_Y292_D0.5530.14
90_V343_V0.5520.13
311_V320_K0.5510.13
113_R253_I0.5510.13
141_M325_R0.5500.13
71_G269_C0.5450.13
52_M73_H0.5450.13
54_T239_L0.5440.13
214_F245_I0.5440.13
256_F309_M0.5440.13
70_D74_N0.5430.13
67_A80_F0.5420.13
236_G297_M0.5400.13
163_T209_A0.5400.13
312_T319_G0.5390.13
273_I311_V0.5390.13
148_Y157_M0.5390.13
236_G311_V0.5360.13
264_E317_T0.5350.13
174_N237_L0.5350.13
63_S137_L0.5340.13
208_I213_G0.5340.13
214_F338_R0.5340.13
214_F243_L0.5300.13
197_G224_L0.5300.13
75_L97_P0.5290.13
187_D287_Y0.5290.13
254_C338_R0.5290.13
209_A354_V0.5270.13
85_V112_E0.5270.13
215_R336_T0.5260.13
8_I13_T0.5260.13
167_R204_M0.5250.13
281_Y311_V0.5250.13
136_Q225_Y0.5230.13
73_H151_E0.5220.13
15_I26_R0.5220.13
44_Q243_L0.5210.13
54_T249_D0.5210.13
168_Y290_V0.5210.13
14_L52_M0.5210.13
28_D68_D0.5200.13
209_A335_R0.5200.13
243_L335_R0.5200.13
292_D341_I0.5200.13
221_K293_L0.5190.13
93_C334_G0.5180.13
213_G236_G0.5180.13
48_Y293_L0.5170.13
147_R305_L0.5170.13
228_D285_F0.5160.13
133_T220_V0.5160.13
3_Q103_C0.5140.13
148_Y329_L0.5140.13
184_V237_L0.5140.13
43_L152_T0.5130.13
278_H307_Y0.5130.13
157_M267_A0.5130.13
5_Y112_E0.5120.13
24_V220_V0.5110.13
150_R153_L0.5110.13
109_K170_H0.5110.13
10_R17_E0.5110.13
12_G17_E0.5110.13
62_Q144_T0.5100.13
174_N221_K0.5090.13
266_L322_D0.5090.13
122_R143_I0.5080.13
259_V348_L0.5080.13
33_E39_E0.5070.12
14_L32_F0.5070.12
13_T127_V0.5060.12
123_A144_T0.5050.12
5_Y340_A0.5040.12
20_S136_Q0.5040.12
74_N238_A0.5030.12
14_L79_I0.5010.12
237_L347_T0.5010.12
22_F113_R0.5010.12
182_V218_I0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4mu3A 6 0.5127 100 0.552 Contact Map
1rhyA 4 0.4901 100 0.554 Contact Map
2ae8A 5 0.4817 100 0.556 Contact Map
4gquA 6 0.5127 100 0.556 Contact Map
2fprA 2 0.4535 100 0.863 Contact Map
3l8hA 1 0.5042 99.9 0.879 Contact Map
2gmwA 1 0.4563 99.9 0.89 Contact Map
4pnhA 4 0.3887 99.8 0.891 Contact Map
2o2xA 1 0.5099 99.8 0.892 Contact Map
3zvlA 1 0.8592 99.8 0.899 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0107 seconds.