GREMLIN Database
AGAL - Alpha-galactosidase
UniProt: P06720 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10577
Length: 451 (414)
Sequences: 1740 (1020)
Seq/√Len: 50.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
73_K139_E5.7961.00
193_T213_K4.6871.00
81_F336_W4.1721.00
76_L141_C3.9791.00
72_Q136_D3.9651.00
104_R436_D3.4981.00
137_M146_M3.3931.00
50_H68_C3.3181.00
369_D374_Q3.2301.00
412_Y434_V3.2241.00
408_R435_D3.2161.00
71_Q74_E3.1431.00
334_A341_S3.1391.00
137_M167_I3.1041.00
73_K136_D3.0781.00
35_H65_K2.9671.00
107_L433_L2.9071.00
345_G368_V2.8791.00
35_H67_T2.8231.00
190_D193_T2.6801.00
354_D380_T2.6641.00
50_H54_R2.6281.00
6_K37_A2.6021.00
292_L300_V2.5541.00
209_E216_D2.5501.00
352_L362_V2.4901.00
381_L389_M2.4901.00
138_T165_P2.4541.00
170_V373_I2.4061.00
405_T410_R2.3921.00
411_V434_V2.3791.00
47_E51_I2.3591.00
37_A67_T2.3421.00
88_I129_H2.3171.00
120_I399_L2.2921.00
132_Q135_E2.2361.00
135_E138_T2.2121.00
105_H432_A2.1961.00
137_M141_C2.1951.00
345_G366_C2.1931.00
134_C160_M2.1681.00
350_D358_Q2.1551.00
212_R222_L2.1231.00
107_L117_P2.0961.00
355_N386_A2.0691.00
138_T166_H2.0641.00
302_Q318_R2.0611.00
356_L389_M2.0541.00
222_L225_E2.0201.00
30_A333_N1.9931.00
253_E276_F1.9861.00
105_H436_D1.9610.99
47_E50_H1.9540.99
73_K140_V1.9460.99
57_M66_I1.9260.99
127_I159_A1.9230.99
108_E285_I1.9100.99
74_E77_E1.9080.99
161_Y348_R1.8810.99
145_T168_K1.8690.99
225_E229_A1.8640.99
401_E405_T1.8570.99
351_G358_Q1.8540.99
131_W159_A1.7820.99
333_N337_T1.7760.99
97_T440_A1.7690.99
21_I36_I1.7610.99
284_L288_Y1.7590.99
401_E410_R1.7330.99
37_A69_H1.7320.99
407_N410_R1.7080.99
127_I155_M1.7000.98
367_L376_T1.6930.98
81_F335_I1.6890.98
6_K35_H1.6850.98
215_A225_E1.6660.98
92_E96_V1.6610.98
340_P369_D1.6470.98
410_R413_H1.6450.98
126_T129_H1.6400.98
335_I338_G1.6350.98
197_R209_E1.5980.98
51_I54_R1.5810.98
143_D168_K1.5770.97
31_L332_M1.5700.97
117_P421_T1.5420.97
120_I395_V1.5330.97
160_M169_Q1.5300.97
80_D143_D1.5200.97
369_D372_G1.5190.97
130_L160_M1.5020.97
355_N379_G1.5010.97
13_G40_D1.4740.96
67_T74_E1.4700.96
354_D379_G1.4560.96
8_T79_A1.4520.96
96_V296_P1.4380.96
314_K317_S1.4360.96
342_V367_L1.4360.96
115_L392_N1.4340.96
317_S320_D1.4330.96
43_P47_E1.4320.96
386_A390_Q1.4240.95
57_M64_G1.4010.95
271_E275_W1.3930.95
134_C137_M1.3850.95
402_A411_V1.3690.94
369_D373_I1.3580.94
433_L437_L1.3570.94
127_I403_I1.3560.94
206_F277_I1.3530.94
78_D143_D1.3490.94
403_I406_E1.3430.94
105_H433_L1.3430.94
354_D358_Q1.3400.94
69_H75_A1.3340.93
131_W404_L1.3280.93
82_V137_M1.3210.93
415_A437_L1.3110.93
101_V440_A1.2960.92
8_T37_A1.2930.92
42_D90_G1.2850.92
301_E305_N1.2740.92
107_L429_E1.2730.92
439_A442_G1.2710.92
8_T76_L1.2660.92
343_I368_V1.2650.92
295_Y299_C1.2620.91
187_L226_L1.2530.91
193_T212_R1.2500.91
371_N374_Q1.2460.91
77_E140_V1.2450.91
84_V130_L1.2430.91
272_Y275_W1.2420.91
7_I31_L1.2350.91
231_E256_K1.2320.90
195_R211_E1.2310.90
212_R215_A1.2290.90
96_V100_E1.2290.90
97_T441_H1.2270.90
199_A381_L1.1990.89
34_A65_K1.1860.89
47_E68_C1.1790.88
355_N390_Q1.1780.88
128_P403_I1.1740.88
199_A208_L1.1700.88
82_V144_A1.1700.88
164_Y167_I1.1650.88
383_S394_N1.1640.88
30_A339_E1.1590.87
415_A430_I1.1540.87
54_R58_D1.1530.87
296_P299_C1.1500.87
273_T277_I1.1330.86
98_D125_R1.1280.86
39_M75_A1.1270.86
384_H413_H1.1230.86
204_M392_N1.1160.85
408_R434_V1.1090.85
76_L140_V1.1070.85
224_P228_A1.1040.85
92_E97_T1.1040.85
354_D377_K1.1030.85
225_E228_A1.1010.85
273_T276_F1.1010.85
5_P336_W1.0950.84
352_L364_V1.0940.84
204_M388_L1.0920.84
342_V376_T1.0830.84
416_M427_I1.0740.83
87_Q95_T1.0740.83
96_V293_D1.0690.83
46_L68_C1.0670.83
271_E276_F1.0590.82
216_D223_Y1.0550.82
107_L422_A1.0520.82
338_G370_A1.0480.82
212_R216_D1.0450.81
412_Y430_I1.0430.81
395_V415_A1.0420.81
154_A396_Q1.0410.81
10_I146_M1.0400.81
121_M438_I1.0340.81
86_F153_M1.0340.81
195_R342_V1.0320.81
95_T99_F1.0310.80
189_I212_R1.0310.80
130_L156_N1.0300.80
158_W397_T1.0270.80
119_G125_R1.0220.80
305_N308_K1.0190.80
114_T119_G1.0170.80
308_K312_E1.0150.79
8_T39_M1.0140.79
53_V66_I1.0090.79
178_G182_E1.0070.79
86_F133_I1.0040.79
408_R438_I1.0040.79
254_M258_L1.0020.79
367_L374_Q0.9990.78
306_W318_R0.9950.78
411_V438_I0.9950.78
421_T430_I0.9930.78
102_C437_L0.9920.78
347_V366_C0.9920.78
198_C207_Y0.9890.78
271_E277_I0.9870.77
408_R431_Y0.9850.77
180_A196_Y0.9830.77
102_C105_H0.9810.77
412_Y431_Y0.9780.77
138_T163_R0.9780.77
342_V365_A0.9760.77
10_I133_I0.9760.77
406_E447_G0.9750.77
109_Q118_G0.9730.76
136_D140_V0.9680.76
274_P277_I0.9660.76
5_P34_A0.9560.75
422_A429_E0.9440.74
135_E164_Y0.9420.74
121_M441_H0.9420.74
43_P70_T0.9410.74
347_V368_V0.9380.74
173_C203_H0.9310.73
330_T341_S0.9310.73
353_I362_V0.9310.73
416_M430_I0.9290.73
134_C166_H0.9290.73
138_T167_I0.9270.73
333_N339_E0.9210.72
282_E286_E0.9130.72
360_C393_I0.9080.71
292_L296_P0.9050.71
156_N159_A0.8970.70
271_E274_P0.8960.70
392_N418_D0.8950.70
198_C205_A0.8940.70
120_I415_A0.8910.70
272_Y276_F0.8910.70
132_Q136_D0.8870.70
250_V254_M0.8790.69
115_L418_D0.8740.68
271_E279_P0.8710.68
81_F332_M0.8700.68
415_A433_L0.8700.68
366_C375_P0.8700.68
123_A127_I0.8670.68
355_N362_V0.8660.68
135_E139_E0.8650.68
294_E298_R0.8580.67
298_R302_Q0.8530.67
360_C397_T0.8470.66
145_T335_I0.8460.66
296_P300_V0.8450.66
102_C433_L0.8450.66
432_A435_D0.8440.66
88_I126_T0.8420.66
149_Y328_A0.8420.66
255_F258_L0.8410.65
79_A141_C0.8410.65
170_V335_I0.8400.65
186_D226_L0.8380.65
228_A232_A0.8380.65
92_E297_K0.8330.65
294_E303_L0.8330.65
180_A207_Y0.8320.65
355_N380_T0.8310.64
368_V373_I0.8260.64
117_P415_A0.8260.64
283_D287_R0.8260.64
163_R167_I0.8220.64
181_E191_P0.8180.63
190_D212_R0.8160.63
87_Q99_F0.8150.63
192_A213_K0.8090.62
129_H132_Q0.8090.62
153_M176_V0.8090.62
300_V304_A0.8090.62
54_R74_E0.8070.62
164_Y404_L0.8060.62
408_R412_Y0.8050.62
216_D232_A0.8050.62
229_A233_G0.8020.62
437_L440_A0.8010.62
189_I193_T0.8010.62
12_A430_I0.7980.61
123_A361_C0.7930.61
54_R65_K0.7930.61
76_L82_V0.7910.61
282_E429_E0.7890.60
252_Y256_K0.7880.60
197_R378_V0.7830.60
9_F83_V0.7830.60
121_M437_L0.7820.60
406_E446_P0.7810.60
438_I442_G0.7790.59
134_C163_R0.7790.59
205_A263_T0.7790.59
98_D119_G0.7780.59
6_K79_A0.7760.59
398_L413_H0.7740.59
44_T358_Q0.7710.59
344_Y365_A0.7710.59
215_A222_L0.7690.58
43_P228_A0.7660.58
102_C118_G0.7660.58
293_D296_P0.7620.58
357_P360_C0.7550.57
158_W393_I0.7510.57
394_N397_T0.7510.57
412_Y415_A0.7510.57
72_Q132_Q0.7500.57
177_Q331_I0.7490.56
147_L332_M0.7490.56
72_Q76_L0.7480.56
275_W284_L0.7480.56
353_I379_G0.7460.56
316_A319_I0.7440.56
184_A189_I0.7430.56
16_I85_A0.7350.55
49_S53_V0.7340.55
387_A394_N0.7330.55
146_M160_M0.7270.54
209_E223_Y0.7260.54
101_V436_D0.7260.54
204_M418_D0.7250.54
432_A436_D0.7250.54
391_T417_M0.7250.54
36_I435_D0.7180.53
275_W278_K0.7170.53
79_A82_V0.7170.53
7_I24_D0.7170.53
109_Q112_A0.7140.53
109_Q396_Q0.7140.53
59_S313_Y0.7140.53
179_T205_A0.7130.53
38_L53_V0.7110.53
47_E374_Q0.7090.52
106_G290_V0.7090.52
114_T155_M0.7080.52
324_S396_Q0.7040.52
139_E228_A0.7040.52
97_T101_V0.7040.52
175_S263_T0.7030.52
186_D252_Y0.7020.52
296_P303_L0.7020.52
56_L144_A0.7010.52
149_Y154_A0.7010.52
334_A343_I0.7010.52
292_L303_L0.6990.51
120_I411_V0.6980.51
157_T171_G0.6980.51
54_R67_T0.6970.51
134_C165_P0.6940.51
174_H324_S0.6920.51
36_I64_G0.6900.50
159_A403_I0.6890.50
69_H74_E0.6880.50
199_A378_V0.6880.50
113_D122_R0.6850.50
254_M262_V0.6850.50
149_Y327_Y0.6850.50
53_V57_M0.6840.50
99_F113_D0.6810.50
161_Y169_Q0.6810.50
304_A307_H0.6780.49
99_F109_Q0.6770.49
275_W279_P0.6760.49
49_S68_C0.6760.49
173_C202_N0.6750.49
23_G157_T0.6750.49
383_S390_Q0.6750.49
146_M169_Q0.6740.49
411_V446_P0.6710.48
135_E165_P0.6710.48
102_C121_M0.6700.48
44_T47_E0.6680.48
206_F413_H0.6660.48
222_L227_L0.6650.48
428_D432_A0.6640.48
251_R254_M0.6630.48
183_L210_L0.6620.48
365_A382_P0.6600.47
58_D194_L0.6570.47
151_N202_N0.6560.47
223_Y226_L0.6550.47
76_L136_D0.6540.47
190_D213_K0.6520.47
364_V378_V0.6520.47
115_L204_M0.6520.47
134_C164_Y0.6520.47
223_Y227_L0.6500.46
198_C344_Y0.6500.46
443_D446_P0.6500.46
44_T351_G0.6490.46
393_I396_Q0.6480.46
126_T403_I0.6470.46
123_A159_A0.6460.46
92_E443_D0.6440.46
57_M63_S0.6390.45
117_P433_L0.6370.45
283_D350_D0.6370.45
355_N381_L0.6370.45
109_Q113_D0.6370.45
12_A50_H0.6370.45
154_A204_M0.6360.45
195_R209_E0.6350.45
389_M413_H0.6350.45
62_A66_I0.6340.45
288_Y291_P0.6340.45
115_L395_V0.6320.44
28_R334_A0.6310.44
25_V334_A0.6300.44
169_Q361_C0.6290.44
67_T139_E0.6280.44
340_P370_A0.6270.44
399_L415_A0.6250.44
17_F85_A0.6220.43
294_E297_K0.6210.43
258_L272_Y0.6200.43
146_M167_I0.6190.43
27_H329_S0.6170.43
114_T125_R0.6170.43
147_L170_V0.6170.43
194_L212_R0.6150.43
26_F326_E0.6150.43
28_R63_S0.6130.43
154_A392_N0.6120.42
292_L298_R0.6120.42
338_G369_D0.6110.42
390_Q394_N0.6110.42
276_F279_P0.6100.42
297_K301_E0.6100.42
46_L70_T0.6100.42
87_Q396_Q0.6090.42
384_H389_M0.6090.42
309_E316_A0.6090.42
51_I68_C0.6080.42
24_D174_H0.6080.42
145_T170_V0.6080.42
149_Y324_S0.6060.42
20_N324_S0.6060.42
358_Q380_T0.6050.42
77_E135_E0.6040.42
15_T304_A0.6020.41
99_F112_A0.6000.41
434_V438_I0.6000.41
14_S19_K0.5990.41
188_N256_K0.5990.41
415_A421_T0.5990.41
134_C167_I0.5990.41
208_L380_T0.5980.41
11_G14_S0.5970.41
82_V141_C0.5970.41
181_E185_R0.5950.41
39_M76_L0.5940.41
256_K259_G0.5930.41
253_E257_K0.5930.41
65_K213_K0.5930.41
273_T279_P0.5910.40
208_L382_P0.5910.40
54_R143_D0.5910.40
304_A308_K0.5890.40
260_Y263_T0.5880.40
150_V444_W0.5880.40
83_V168_K0.5870.40
76_L115_L0.5860.40
30_A332_M0.5850.40
133_I146_M0.5840.40
409_D413_H0.5840.40
175_S179_T0.5830.40
196_Y207_Y0.5830.40
10_I76_L0.5820.40
228_A256_K0.5810.39
253_E259_G0.5800.39
6_K78_D0.5800.39
17_F20_N0.5790.39
87_Q122_R0.5790.39
131_W164_Y0.5770.39
315_K322_K0.5770.39
48_E311_E0.5760.39
163_R401_E0.5760.39
126_T156_N0.5760.39
109_Q153_M0.5740.39
87_Q109_Q0.5740.39
333_N366_C0.5710.38
192_A215_A0.5700.38
151_N203_H0.5700.38
16_I19_K0.5690.38
254_M273_T0.5680.38
382_P385_L0.5680.38
208_L260_Y0.5670.38
180_A365_A0.5660.38
7_I332_M0.5650.38
155_M423_A0.5640.38
104_R432_A0.5640.38
175_S205_A0.5640.38
99_F396_Q0.5630.38
120_I123_A0.5620.38
121_M141_C0.5620.38
226_L230_Y0.5610.37
116_G125_R0.5610.37
58_D225_E0.5600.37
16_I91_Y0.5600.37
305_N311_E0.5590.37
115_L155_M0.5580.37
180_A210_L0.5580.37
19_K324_S0.5580.37
273_T285_I0.5570.37
180_A194_L0.5570.37
223_Y230_Y0.5570.37
36_I74_E0.5570.37
279_P283_D0.5560.37
332_M379_G0.5550.37
131_W135_E0.5550.37
10_I41_I0.5540.37
12_A53_V0.5530.37
84_V150_V0.5530.37
77_E350_D0.5520.37
122_R126_T0.5510.37
213_K405_T0.5510.37
184_A206_F0.5500.36
135_E166_H0.5500.36
95_T114_T0.5490.36
114_T276_F0.5490.36
121_M390_Q0.5480.36
95_T441_H0.5480.36
13_G41_I0.5480.36
151_N173_C0.5470.36
65_K442_G0.5470.36
433_L436_D0.5460.36
431_Y435_D0.5460.36
34_A63_S0.5460.36
298_R301_E0.5460.36
54_R92_E0.5460.36
216_D397_T0.5450.36
403_I445_L0.5450.36
199_A362_V0.5430.36
309_E314_K0.5430.36
309_E312_E0.5420.36
84_V146_M0.5420.36
24_D332_M0.5420.36
175_S327_Y0.5410.36
124_L403_I0.5400.35
8_T67_T0.5400.35
285_I288_Y0.5390.35
103_K285_I0.5380.35
20_N99_F0.5370.35
85_A296_P0.5370.35
335_I339_E0.5350.35
364_V375_P0.5340.35
392_N396_Q0.5340.35
48_E406_E0.5340.35
52_V59_S0.5340.35
155_M400_T0.5340.35
155_M403_I0.5330.35
352_L376_T0.5330.35
176_V344_Y0.5320.35
155_M338_G0.5320.35
95_T109_Q0.5310.35
286_E435_D0.5300.35
395_V418_D0.5290.34
16_I87_Q0.5290.34
395_V399_L0.5290.34
256_K358_Q0.5290.34
7_I36_I0.5280.34
86_F347_V0.5270.34
197_R208_L0.5270.34
319_I388_L0.5260.34
10_I72_Q0.5260.34
8_T77_E0.5250.34
74_E439_A0.5250.34
114_T122_R0.5240.34
96_V297_K0.5240.34
330_T343_I0.5240.34
231_E439_A0.5240.34
350_D375_P0.5230.34
118_G125_R0.5230.34
38_L66_I0.5220.34
98_D116_G0.5220.34
99_F114_T0.5210.34
65_K190_D0.5200.34
140_V193_T0.5200.34
112_A396_Q0.5190.34
187_L225_E0.5180.33
359_G435_D0.5180.33
53_V85_A0.5170.33
357_P397_T0.5170.33
10_I39_M0.5170.33
342_V374_Q0.5160.33
275_W285_I0.5150.33
63_S413_H0.5140.33
19_K325_R0.5120.33
29_E51_I0.5110.33
52_V197_R0.5110.33
386_A397_T0.5100.33
406_E436_D0.5100.33
54_R187_L0.5090.33
189_I194_L0.5090.33
230_Y247_Q0.5090.33
125_R414_A0.5080.33
180_A191_P0.5080.33
28_R333_N0.5080.33
398_L414_A0.5070.32
56_L60_A0.5070.32
171_G419_P0.5060.32
398_L417_M0.5060.32
84_V169_Q0.5060.32
407_N447_G0.5050.32
28_R64_G0.5050.32
105_H117_P0.5050.32
87_Q103_K0.5050.32
163_R166_H0.5050.32
224_P364_V0.5030.32
95_T112_A0.5030.32
254_M257_K0.5030.32
19_K23_G0.5020.32
352_L359_G0.5020.32
158_W400_T0.5010.32
43_P139_E0.5000.32
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1obbA 2 0.949 100 0.368 Contact Map
1s6yA 1 0.8914 100 0.37 Contact Map
1u8xX 1 0.9357 100 0.376 Contact Map
1up7A 3 0.9047 100 0.389 Contact Map
3fefA 3 0.929 100 0.403 Contact Map
3u95A 2 0.9446 100 0.428 Contact Map
1pzgA 4 0.7007 100 0.72 Contact Map
4rorA 4 0.6962 100 0.728 Contact Map
3p7mA 4 0.6763 100 0.729 Contact Map
2hjrA 4 0.6696 100 0.731 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0129 seconds.