GREMLIN Database
BIRA - Bifunctional ligase/repressor BirA
UniProt: P06709 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10123
Length: 321 (297)
Sequences: 2743 (2095)
Seq/√Len: 121.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
290_R301_E3.8771.00
159_E264_R3.7381.00
87_I93_Y3.4791.00
159_E245_E3.3991.00
302_Q305_I3.3791.00
86_V111_Y3.2161.00
163_K245_E2.9841.00
93_Y96_D2.9241.00
94_L136_F2.8061.00
170_R270_N2.7831.00
133_L239_A2.7071.00
41_Q44_R2.6811.00
84_L87_I2.6641.00
131_L239_A2.6401.00
162_R268_L2.5481.00
134_S202_V2.5361.00
125_S132_Y2.5321.00
266_E291_G2.5081.00
293_D297_A2.4991.00
88_D92_Q2.4401.00
110_E235_R2.4151.00
23_E27_E2.3791.00
158_A268_L2.3651.00
108_I239_A2.3281.00
277_K286_F2.2931.00
88_D93_Y2.1731.00
159_E249_A2.1511.00
163_K241_M2.1311.00
275_P302_Q2.0971.00
179_L184_L2.0821.00
157_M185_A2.0801.00
75_Q240_A2.0801.00
299_L306_I2.0211.00
106_A243_I2.0121.00
157_M242_L1.9881.00
154_G171_V1.9491.00
106_A135_M1.9441.00
94_L107_C1.9361.00
293_D308_P1.9291.00
262_L266_E1.9051.00
153_I203_I1.8971.00
277_K284_E1.8781.00
88_D96_D1.8551.00
162_R168_K1.7931.00
275_P288_I1.7871.00
271_F289_S1.7781.00
290_R306_I1.7571.00
156_V249_A1.7551.00
149_L201_I1.7311.00
84_L93_Y1.7231.00
275_P286_F1.6971.00
291_G299_L1.6951.00
301_E306_I1.6931.00
98_I136_F1.6731.00
272_I291_G1.6621.00
278_L314_I1.6311.00
299_L308_P1.6251.00
160_V242_L1.5911.00
53_V56_K1.5481.00
157_M177_L1.5441.00
97_R107_C1.5331.00
65_Q234_D1.5191.00
288_I301_E1.5121.00
103_S250_L1.4951.00
22_G33_R1.4941.00
125_S130_N1.4831.00
278_L309_W1.4561.00
160_V245_E1.4261.00
169_V177_L1.4091.00
249_A264_R1.3841.00
82_A97_R1.3561.00
51_F59_S1.3551.00
178_Y270_N1.3481.00
130_N211_M1.3331.00
51_F61_P1.3271.00
179_L228_E1.3201.00
234_D237_T1.3201.00
158_A169_V1.3041.00
35_A38_K1.2991.00
207_I242_L1.2921.00
101_L136_F1.2921.00
223_W228_E1.2801.00
238_L242_L1.2751.00
205_A242_L1.2741.00
177_L185_A1.2721.00
69_A73_L1.2701.00
216_E219_V1.2560.99
96_D100_E1.2560.99
222_G225_T1.2500.99
24_Q28_T1.2420.99
181_D274_R1.2370.99
153_I205_A1.2110.99
276_V289_S1.2090.99
68_N71_Q1.1930.99
162_R169_V1.1900.99
78_G247_R1.1850.99
93_Y97_R1.1790.99
91_N202_V1.1760.99
241_M244_R1.1760.99
297_A308_P1.1640.99
156_V160_V1.1610.99
102_K105_D1.1610.99
108_I133_L1.1600.99
245_E248_A1.1600.99
86_V113_Q1.1590.99
169_V179_L1.1520.99
297_A310_M1.1510.99
77_D244_R1.1460.99
278_L300_L1.1410.99
269_D289_S1.1400.99
192_T200_Q1.1350.99
209_M238_L1.1330.99
295_Q308_P1.1300.99
223_W227_Q1.1280.99
269_D272_I1.1250.99
265_W269_D1.1250.99
273_N290_R1.1160.99
185_A207_I1.1100.99
161_L165_G1.1090.99
189_V201_I1.1030.99
123_W174_P1.0930.99
72_I236_N1.0930.99
150_S189_V1.0920.99
151_L296_G1.0880.99
72_I75_Q1.0880.99
246_L250_L1.0680.98
82_A105_D1.0540.98
279_I284_E1.0500.98
49_D62_E1.0500.98
258_L262_L1.0350.98
126_P132_Y1.0330.98
126_P129_A1.0330.98
164_L241_M1.0280.98
67_L83_V1.0270.98
266_E272_I1.0260.98
252_L256_E1.0200.98
79_G104_G1.0140.98
236_N240_A1.0100.98
33_R57_G1.0070.98
135_M246_L1.0050.98
152_V249_A1.0050.98
241_M245_E1.0040.98
33_R38_K0.9990.97
263_S267_K0.9900.97
84_L107_C0.9860.97
180_Q228_E0.9830.97
157_M205_A0.9710.97
215_E218_V0.9710.97
22_G57_G0.9680.97
104_G247_R0.9680.97
242_L246_L0.9580.97
22_G58_Y0.9570.97
210_A226_L0.9550.97
69_A83_V0.9450.96
33_R58_Y0.9440.96
34_A37_N0.9430.96
24_Q27_E0.9430.96
95_L202_V0.9400.96
249_A252_L0.9340.96
101_L105_D0.9330.96
40_I50_V0.9320.96
155_I249_A0.9310.96
245_E249_A0.9290.96
160_V164_L0.9280.96
240_A244_R0.9240.96
97_R105_D0.9210.96
102_K138_R0.9170.96
101_L104_G0.9150.96
276_V316_L0.9130.96
121_R313_E0.9110.96
115_G125_S0.9110.96
106_A239_A0.9070.96
290_R299_L0.9050.95
78_G104_G0.9050.95
287_G309_W0.8980.95
22_G38_K0.8970.95
271_F314_I0.8920.95
219_V222_G0.8870.95
94_L134_S0.8870.95
25_L36_I0.8860.95
105_D136_F0.8810.95
285_I307_K0.8790.95
22_G35_A0.8750.95
106_A133_L0.8720.94
133_L242_L0.8700.94
221_Q225_T0.8700.94
203_I250_L0.8700.94
85_P110_E0.8680.94
22_G37_N0.8680.94
252_L264_R0.8670.94
223_W226_L0.8650.94
158_A162_R0.8640.94
91_N312_G0.8640.94
218_V221_Q0.8610.94
264_R267_K0.8570.94
170_R181_D0.8530.94
300_L309_W0.8530.94
224_I227_Q0.8520.94
82_A93_Y0.8510.94
161_L169_V0.8510.94
278_L285_I0.8510.94
279_I317_R0.8510.94
278_L312_G0.8500.94
79_G247_R0.8490.94
218_V222_G0.8480.94
279_I282_D0.8470.94
211_M214_V0.8460.94
98_I200_Q0.8460.94
158_A177_L0.8400.93
135_M243_I0.8370.93
264_R268_L0.8300.93
155_I265_W0.8280.93
279_I315_S0.8270.93
203_I246_L0.8270.93
71_Q234_D0.8270.93
153_I246_L0.8270.93
263_S266_E0.8260.93
212_R226_L0.8250.93
262_L294_K0.8220.93
161_L184_L0.8210.92
152_V155_I0.8200.92
110_E128_G0.8200.92
261_Y265_W0.8190.92
111_Y125_S0.8140.92
103_S137_W0.8110.92
259_A294_K0.8110.92
94_L105_D0.8080.92
33_R37_N0.8080.92
189_V203_I0.8020.92
274_R289_S0.8000.91
131_L209_M0.8000.91
184_L223_W0.8000.91
161_L238_L0.7980.91
285_I309_W0.7980.91
35_A39_H0.7960.91
49_D61_P0.7960.91
237_T240_A0.7940.91
258_L292_I0.7930.91
271_F291_G0.7920.91
237_T241_M0.7890.91
239_A243_I0.7850.91
272_I289_S0.7830.91
22_G26_G0.7820.91
124_F215_E0.7750.90
137_W250_L0.7740.90
146_A198_A0.7710.90
21_S33_R0.7690.90
148_G253_F0.7690.90
161_L166_A0.7690.90
287_G300_L0.7680.90
93_Y107_C0.7680.90
207_I238_L0.7660.90
265_W292_I0.7650.90
42_T45_D0.7600.89
146_A191_L0.7590.89
209_M235_R0.7550.89
83_V236_N0.7550.89
155_I264_R0.7530.89
80_S105_D0.7430.88
22_G32_S0.7430.88
137_W203_I0.7420.88
85_P127_F0.7420.88
219_V223_W0.7400.88
226_L232_N0.7340.88
133_L205_A0.7340.88
37_N194_K0.7320.87
151_L154_G0.7290.87
210_A224_I0.7240.87
249_A261_Y0.7180.86
155_I261_Y0.7150.86
158_A171_V0.7140.86
72_I239_A0.7130.86
111_Y124_F0.7100.86
71_Q74_G0.7060.85
214_V217_S0.7050.85
50_V59_S0.7050.85
149_L250_L0.7040.85
82_A87_I0.7000.85
280_I283_K0.7000.85
248_A252_L0.6980.85
269_D291_G0.6960.85
111_Y127_F0.6950.85
109_A132_Y0.6950.85
44_R52_T0.6950.85
166_A169_V0.6930.84
178_Y271_F0.6910.84
92_Q114_A0.6890.84
161_L177_L0.6850.84
132_Y211_M0.6820.83
210_A231_I0.6820.83
72_I240_A0.6810.83
37_N58_Y0.6810.83
168_K180_Q0.6800.83
129_A211_M0.6800.83
138_R200_Q0.6790.83
160_V241_M0.6780.83
139_L250_L0.6770.83
31_M39_H0.6760.83
31_M35_A0.6750.83
155_I159_E0.6710.82
147_I150_S0.6690.82
23_E34_A0.6670.82
225_T228_E0.6550.81
92_Q96_D0.6550.81
72_I81_V0.6550.81
37_N41_Q0.6540.81
38_K58_Y0.6540.81
101_L138_R0.6530.81
177_L184_L0.6520.81
48_V64_I0.6510.81
300_L316_L0.6490.80
191_L201_I0.6470.80
244_R248_A0.6410.80
141_Q253_F0.6400.79
155_I268_L0.6400.79
148_G258_L0.6370.79
257_G260_P0.6370.79
22_G34_A0.6370.79
182_R228_E0.6370.79
123_W151_L0.6370.79
104_G250_L0.6340.79
48_V60_L0.6280.78
44_R49_D0.6270.78
104_G136_F0.6270.78
142_G145_A0.6260.78
31_M34_A0.6240.78
220_N223_W0.6230.78
71_Q76_L0.6210.77
153_I189_V0.6210.77
143_P191_L0.6210.77
224_I229_A0.6200.77
35_A57_G0.6180.77
136_F200_Q0.6180.77
22_G36_I0.6180.77
225_T229_A0.6170.77
219_V225_T0.6140.77
285_I300_L0.6130.76
214_V218_V0.6120.76
69_A72_I0.6120.76
39_H42_T0.6120.76
287_G296_G0.6120.76
33_R36_I0.6110.76
251_E254_E0.6100.76
194_K197_D0.6100.76
133_L243_I0.6090.76
76_L240_A0.6020.75
91_N134_S0.6020.75
266_E269_D0.5980.75
95_L192_T0.5970.75
26_G29_L0.5960.74
220_N224_I0.5950.74
152_V253_F0.5940.74
39_H265_W0.5940.74
90_T109_A0.5910.74
123_W296_G0.5900.74
277_K317_R0.5900.74
60_L63_P0.5890.74
139_L198_A0.5890.74
161_L167_D0.5890.74
243_I247_R0.5870.73
103_S138_R0.5860.73
148_G152_V0.5840.73
92_Q95_L0.5830.73
160_V238_L0.5820.73
256_E260_P0.5820.73
103_S254_E0.5800.72
31_M36_I0.5790.72
298_L309_W0.5780.72
163_K244_R0.5780.72
165_G168_K0.5770.72
280_I307_K0.5760.72
153_I156_V0.5750.72
265_W310_M0.5740.72
210_A230_G0.5720.71
123_W191_L0.5690.71
37_N57_G0.5680.71
271_F274_R0.5670.71
38_K57_G0.5670.71
276_V309_W0.5670.71
141_Q145_A0.5650.71
150_S154_G0.5640.70
97_R101_L0.5600.70
67_L239_A0.5570.69
126_P211_M0.5570.69
289_S298_L0.5570.69
136_F139_L0.5570.69
123_W195_T0.5550.69
52_T59_S0.5530.69
217_S221_Q0.5520.69
92_Q116_R0.5510.69
159_E268_L0.5510.69
87_I109_A0.5510.69
68_N74_G0.5480.68
289_S300_L0.5460.68
266_E292_I0.5440.68
304_G307_K0.5430.67
68_N234_D0.5430.67
156_V245_E0.5430.67
67_L72_I0.5430.67
68_N236_N0.5430.67
258_L266_E0.5430.67
210_A233_L0.5420.67
107_C136_F0.5420.67
211_M224_I0.5400.67
161_L207_I0.5380.67
81_V106_A0.5380.67
83_V108_I0.5360.67
178_Y265_W0.5360.67
135_M247_R0.5350.66
286_F302_Q0.5340.66
35_A58_Y0.5340.66
178_Y316_L0.5330.66
229_A233_L0.5330.66
145_A148_G0.5320.66
147_I151_L0.5310.66
104_G137_W0.5310.66
181_D270_N0.5310.66
20_H28_T0.5300.66
32_S35_A0.5280.65
278_L313_E0.5280.65
157_M207_I0.5260.65
123_W210_A0.5240.65
149_L253_F0.5240.65
72_I237_T0.5230.65
152_V201_I0.5230.65
84_L88_D0.5220.64
98_I101_L0.5210.64
48_V71_Q0.5210.64
116_R190_E0.5210.64
181_D268_L0.5200.64
111_Y132_Y0.5200.64
293_D299_L0.5180.64
233_L238_L0.5180.64
88_D109_A0.5160.64
120_G313_E0.5160.64
174_P265_W0.5150.63
162_R267_K0.5150.63
252_L260_P0.5150.63
174_P296_G0.5120.63
23_E42_T0.5060.62
83_V94_L0.5060.62
80_S102_K0.5010.61
94_L97_R0.5010.61
139_L146_A0.5010.61
151_L174_P0.5000.61
41_Q45_D0.5000.61
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1biaA 1 0.9097 100 0.163 Contact Map
3rkxA 1 0.9283 100 0.191 Contact Map
2eayA 2 0.6885 100 0.34 Contact Map
2dxuA 2 0.7165 100 0.346 Contact Map
4op0A 1 0.7726 100 0.35 Contact Map
2ej9A 1 0.7134 100 0.357 Contact Map
3bfmA 2 0.7009 100 0.477 Contact Map
1vqzA 1 0.7664 99.9 0.76 Contact Map
2p5iA 1 0.6729 99.9 0.781 Contact Map
2p0lA 1 0.7321 99.7 0.825 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0099 seconds.