FTSQ - ECOLI - GREMLIN Contacts

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FTSQ - Cell division protein FtsQ

UniProt:	P06136	Sequences: MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWVVLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQRLPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPMLYGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMKRLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10342
Length:	276 (229)
Sequences:	3130 (2580)
Seq/√Len:	170.5

ECOLI - FTSQ
BACSU - DIVIB

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
126_Y	139_D	4.967	1.00
114_Q	120_K	3.519	1.00
63_T	122_H	2.989	1.00
70_R	73_D	2.839	1.00
100_E	109_V	2.838	1.00
79_L	121_I	2.802	1.00
92_V	113_K	2.744	1.00
62_L	74_I	2.521	1.00
92_V	111_V	2.494	1.00
61_V	120_K	2.486	1.00
59_K	118_E	2.458	1.00
107_K	141_E	2.435	1.00
71_N	75_R	2.374	1.00
78_I	103_L	2.373	1.00
69_T	103_L	2.312	1.00
202_T	208_K	2.300	1.00
107_K	124_V	2.284	1.00
61_V	118_E	2.279	1.00
126_Y	143_N	2.278	1.00
91_D	94_I	2.213	1.00
99_I	123_L	2.144	1.00
209_L	223_F	2.143	1.00
212_G	219_R	2.109	1.00
100_E	108_Q	2.098	1.00
63_T	120_K	2.098	1.00
220_L	228_P	2.089	1.00
96_Q	100_E	2.016	1.00
95_I	111_V	1.934	1.00
187_T	204_N	1.916	1.00
81_L	98_Q	1.907	1.00
203_L	209_L	1.877	1.00
107_K	140_A	1.874	1.00
72_D	76_Q	1.822	1.00
99_I	106_I	1.813	1.00
87_F	115_W	1.771	1.00
139_D	143_N	1.750	1.00
243_Y	255_G	1.742	1.00
107_K	126_Y	1.727	1.00
66_R	125_E	1.715	1.00
108_Q	124_V	1.672	1.00
200_Q	208_K	1.619	1.00
58_S	117_D	1.564	1.00
94_I	98_Q	1.561	1.00
110_S	122_H	1.555	1.00
112_R	122_H	1.547	1.00
55_L	88_M	1.533	1.00
92_V	96_Q	1.533	1.00
210_N	243_Y	1.529	1.00
96_Q	111_V	1.505	1.00
161_Y	190_E	1.483	1.00
161_Y	192_A	1.483	1.00
220_L	226_L	1.471	1.00
68_Y	105_W	1.461	1.00
62_L	75_R	1.447	1.00
215_D	218_K	1.439	1.00
203_L	206_D	1.438	1.00
208_K	243_Y	1.419	1.00
130_A	160_L	1.412	1.00
223_F	244_V	1.411	1.00
29_L	33_T	1.410	1.00
112_R	120_K	1.393	1.00
106_I	109_V	1.386	1.00
105_W	145_F	1.383	1.00
220_L	224_V	1.375	1.00
198_S	212_G	1.371	1.00
220_L	223_F	1.370	1.00
176_E	217_M	1.362	1.00
219_R	223_F	1.359	1.00
79_L	119_L	1.337	1.00
68_Y	125_E	1.333	1.00
66_R	71_N	1.321	1.00
22_G	26_A	1.321	1.00
129_I	140_A	1.305	1.00
72_D	75_R	1.300	1.00
189_K	202_T	1.297	1.00
130_A	138_V	1.290	1.00
136_H	144_T	1.286	1.00
223_F	246_L	1.286	1.00
75_R	79_L	1.271	1.00
176_E	180_M	1.253	1.00
66_R	74_I	1.235	1.00
195_A	198_S	1.229	1.00
97_T	100_E	1.213	1.00
103_L	106_I	1.179	1.00
223_F	227_Y	1.175	1.00
173_G	176_E	1.163	1.00
189_K	204_N	1.140	1.00
93_N	96_Q	1.138	1.00
172_Q	176_E	1.129	1.00
173_G	191_A	1.126	1.00
109_V	121_I	1.120	1.00
190_E	202_T	1.114	1.00
188_L	201_L	1.112	1.00
73_D	78_I	1.105	1.00
87_F	113_K	1.101	1.00
24_R	27_G	1.090	1.00
36_T	40_V	1.082	1.00
223_F	228_P	1.082	1.00
26_A	29_L	1.079	1.00
66_R	69_T	1.079	1.00
55_L	86_T	1.075	1.00
19_R	23_T	1.073	1.00
194_T	198_S	1.073	1.00
74_I	123_L	1.072	1.00
96_Q	108_Q	1.066	1.00
21_N	25_L	1.063	1.00
219_R	226_L	1.061	1.00
22_G	25_L	1.055	0.99
248_Y	251_G	1.053	0.99
133_N	159_M	1.052	0.99
69_T	99_I	1.051	0.99
128_P	157_L	1.046	0.99
242_S	255_G	1.037	0.99
175_R	178_G	1.031	0.99
35_L	39_L	1.027	0.99
207_I	241_I	1.016	0.99
79_L	99_I	1.006	0.99
174_Y	178_G	1.002	0.99
29_L	32_L	0.981	0.99
52_A	84_P	0.975	0.99
57_L	90_Q	0.968	0.99
219_R	222_R	0.964	0.99
28_I	32_L	0.953	0.99
90_Q	119_L	0.949	0.99
201_L	209_L	0.949	0.99
200_Q	210_N	0.943	0.99
21_N	24_R	0.942	0.99
194_T	212_G	0.940	0.99
219_R	224_V	0.936	0.99
222_R	226_L	0.936	0.99
167_A	170_V	0.929	0.99
79_L	95_I	0.922	0.99
106_I	123_L	0.918	0.99
81_L	119_L	0.917	0.99
210_N	245_D	0.915	0.99
111_V	121_I	0.910	0.98
248_Y	253_A	0.908	0.98
34_V	38_V	0.904	0.98
26_A	30_F	0.904	0.98
184_D	203_L	0.903	0.98
131_R	159_M	0.893	0.98
75_R	80_A	0.891	0.98
25_L	28_I	0.889	0.98
42_G	46_L	0.889	0.98
90_Q	95_I	0.882	0.98
127_V	140_A	0.878	0.98
59_K	117_D	0.869	0.98
207_I	244_V	0.863	0.98
43_W	47_G	0.862	0.98
19_R	22_G	0.861	0.98
241_I	254_V	0.860	0.98
159_M	190_E	0.858	0.98
46_L	50_E	0.854	0.98
217_M	220_L	0.851	0.98
137_M	145_F	0.845	0.98
105_W	130_A	0.844	0.98
87_F	117_D	0.842	0.97
33_T	37_T	0.835	0.97
30_F	40_V	0.831	0.97
40_V	44_V	0.827	0.97
31_L	35_L	0.826	0.97
191_A	201_L	0.822	0.97
66_R	123_L	0.822	0.97
26_A	32_L	0.821	0.97
114_Q	118_E	0.817	0.97
105_W	146_S	0.814	0.97
41_S	44_V	0.811	0.97
68_Y	128_P	0.805	0.97
25_L	35_L	0.803	0.97
226_L	252_A	0.803	0.97
201_L	211_L	0.799	0.97
23_T	26_A	0.797	0.97
171_L	175_R	0.794	0.96
113_K	119_L	0.794	0.96
69_T	105_W	0.793	0.96
132_W	199_W	0.782	0.96
20_N	23_T	0.780	0.96
211_L	215_D	0.779	0.96
173_G	177_M	0.779	0.96
82_G	98_Q	0.778	0.96
69_T	74_I	0.773	0.96
86_T	89_T	0.772	0.96
105_W	148_P	0.770	0.96
95_I	99_I	0.769	0.96
251_G	254_V	0.766	0.96
41_S	45_V	0.765	0.96
109_V	113_K	0.762	0.96
30_F	33_T	0.762	0.96
192_A	200_Q	0.759	0.95
20_N	33_T	0.756	0.95
93_N	97_T	0.754	0.95
31_L	42_G	0.754	0.95
173_G	178_G	0.750	0.95
224_V	228_P	0.748	0.95
87_F	119_L	0.737	0.95
176_E	181_L	0.731	0.94
57_L	139_D	0.730	0.94
22_G	42_G	0.727	0.94
95_I	98_Q	0.726	0.94
20_N	25_L	0.725	0.94
194_T	200_Q	0.719	0.94
145_F	163_P	0.719	0.94
31_L	37_T	0.718	0.94
173_G	193_M	0.718	0.94
26_A	40_V	0.717	0.94
219_R	228_P	0.712	0.94
19_R	31_L	0.707	0.93
252_A	255_G	0.706	0.93
221_A	225_E	0.706	0.93
178_G	182_A	0.698	0.93
63_T	66_R	0.694	0.93
113_K	116_P	0.693	0.93
42_G	45_V	0.688	0.92
30_F	41_S	0.688	0.92
131_R	137_M	0.688	0.92
151_R	154_K	0.686	0.92
67_H	125_E	0.684	0.92
25_L	29_L	0.683	0.92
98_Q	102_R	0.682	0.92
83_E	90_Q	0.678	0.92
193_M	196_R	0.678	0.92
50_E	53_Q	0.677	0.92
19_R	24_R	0.676	0.92
31_L	34_V	0.665	0.91
23_T	27_G	0.662	0.91
113_K	145_F	0.662	0.91
164_E	167_A	0.660	0.91
96_Q	199_W	0.660	0.91
56_P	85_G	0.658	0.91
136_H	148_P	0.657	0.91
145_F	191_A	0.655	0.90
159_M	189_K	0.654	0.90
172_Q	218_K	0.651	0.90
134_D	156_V	0.649	0.90
38_V	41_S	0.646	0.90
131_R	157_L	0.646	0.90
99_I	121_I	0.644	0.90
225_E	229_V	0.637	0.89
225_E	228_P	0.635	0.89
20_N	26_A	0.633	0.89
99_I	109_V	0.633	0.89
79_L	98_Q	0.630	0.89
92_V	184_D	0.628	0.89
164_E	182_A	0.625	0.88
113_K	136_H	0.623	0.88
128_P	137_M	0.620	0.88
194_T	219_R	0.615	0.87
132_W	248_Y	0.615	0.87
222_R	249_D	0.614	0.87
192_A	198_S	0.614	0.87
74_I	106_I	0.612	0.87
198_S	210_N	0.611	0.87
249_D	252_A	0.609	0.87
56_P	89_T	0.608	0.87
170_V	173_G	0.608	0.87
220_L	227_Y	0.607	0.87
69_T	123_L	0.604	0.87
125_E	203_L	0.604	0.87
64_G	121_I	0.602	0.86
25_L	32_L	0.600	0.86
29_L	40_V	0.598	0.86
27_G	31_L	0.597	0.86
170_V	178_G	0.596	0.86
237_D	240_R	0.595	0.86
95_I	119_L	0.595	0.86
80_A	98_Q	0.593	0.86
199_W	211_L	0.593	0.86
176_E	179_Q	0.593	0.86
28_I	97_T	0.592	0.85
88_M	113_K	0.591	0.85
247_R	253_A	0.590	0.85
99_I	103_L	0.587	0.85
132_W	247_R	0.586	0.85
60_L	251_G	0.585	0.85
32_L	39_L	0.585	0.85
25_L	38_V	0.582	0.84
69_T	146_S	0.580	0.84
241_I	244_V	0.579	0.84
131_R	219_R	0.579	0.84
69_T	126_Y	0.577	0.84
21_N	26_A	0.577	0.84
199_W	213_R	0.576	0.84
25_L	39_L	0.574	0.84
220_L	249_D	0.573	0.84
34_V	41_S	0.573	0.84
61_V	122_H	0.571	0.83
115_W	145_F	0.570	0.83
20_N	24_R	0.570	0.83
70_R	204_N	0.567	0.83
173_G	181_L	0.566	0.83
20_N	28_I	0.565	0.83
150_E	154_K	0.563	0.82
105_W	220_L	0.562	0.82
226_L	241_I	0.562	0.82
180_M	223_F	0.559	0.82
193_M	198_S	0.558	0.82
244_V	248_Y	0.555	0.82
203_L	207_I	0.554	0.81
198_S	213_R	0.550	0.81
24_R	31_L	0.548	0.81
162_G	219_R	0.547	0.81
224_V	244_V	0.546	0.81
75_R	81_L	0.546	0.81
88_M	249_D	0.545	0.80
23_T	28_I	0.545	0.80
81_L	95_I	0.544	0.80
61_V	114_Q	0.543	0.80
33_T	43_W	0.540	0.80
63_T	218_K	0.539	0.80
125_E	201_L	0.539	0.80
31_L	38_V	0.535	0.79
53_Q	58_S	0.533	0.79
165_G	170_V	0.530	0.79
20_N	29_L	0.530	0.79
238_G	244_V	0.530	0.79
39_L	43_W	0.529	0.78
211_L	246_L	0.528	0.78
129_I	171_L	0.528	0.78
69_T	125_E	0.526	0.78
129_I	137_M	0.525	0.78
128_P	145_F	0.525	0.78
196_R	213_R	0.524	0.78
31_L	97_T	0.522	0.78
45_V	72_D	0.522	0.78
78_I	99_I	0.520	0.77
217_M	224_V	0.520	0.77
130_A	157_L	0.520	0.77
71_N	104_P	0.519	0.77
23_T	40_V	0.518	0.77
189_K	206_D	0.517	0.77
34_V	62_L	0.515	0.77
37_T	41_S	0.513	0.76
133_N	161_Y	0.513	0.76
81_L	90_Q	0.513	0.76
211_L	220_L	0.513	0.76
55_L	139_D	0.509	0.76
28_I	31_L	0.508	0.76
71_N	120_K	0.506	0.75
82_G	85_G	0.505	0.75
105_W	113_K	0.505	0.75
86_T	115_W	0.504	0.75
28_I	43_W	0.502	0.75
192_A	202_T	0.501	0.75
92_V	115_W	0.500	0.75

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2vh2A	1	0.7246	100	0.292	Contact Map
2vh1A	1	0.7246	100	0.317	Contact Map
1yr1A	1	0.4022	99.4	0.723	Contact Map
3j00Z	1	0.3514	99.1	0.755	Contact Map
4k3bA	1	0.7464	95	0.882	Contact Map
3efcA	1	0.7464	88.4	0.903	Contact Map
2qdfA	1	0.7609	86.2	0.907	Contact Map
2x8xX	1	0.7645	76.3	0.916	Contact Map
3q6bA	1	0.413	49.9	0.929	Contact Map
2kogA	1	0.1884	45.4	0.931	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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