GREMLIN Database
TSAD - tRNA N6-adenosine threonylcarbamoyltransferase
UniProt: P05852 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11171
Length: 337 (327)
Sequences: 4297 (2433)
Seq/√Len: 134.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
146_T150_Q3.8671.00
5_G306_A3.2581.00
61_A65_E3.2571.00
122_E294_R3.1561.00
77_A309_G3.0701.00
207_L257_Q3.0041.00
114_G305_I2.9931.00
19_D23_G2.9131.00
190_G234_D2.6991.00
170_A179_G2.6131.00
144_S260_F2.5961.00
2_R68_L2.5261.00
59_I94_V2.4811.00
76_V98_L2.4441.00
146_T152_E2.4131.00
176_D182_L2.3931.00
78_Y106_A2.3381.00
184_S273_R2.2891.00
241_D274_T2.2851.00
2_R19_D2.2541.00
262_R289_E2.1901.00
73_I104_V2.1711.00
197_F221_T2.1461.00
280_A284_K2.1271.00
219_A223_R2.1121.00
70_A102_W2.0701.00
28_Q61_A2.0501.00
256_D286_R2.0151.00
188_A273_R2.0141.00
169_T218_A1.9921.00
255_L263_L1.9921.00
15_I58_L1.9891.00
9_S79_T1.9841.00
199_R245_D1.9811.00
195_F221_T1.9741.00
239_F243_V1.9551.00
183_L236_A1.8961.00
251_C263_L1.8871.00
310_M314_K1.8851.00
60_Q64_K1.8711.00
50_D53_R1.8691.00
15_I59_I1.8221.00
114_G308_A1.7941.00
99_A106_A1.7851.00
3_V75_A1.7781.00
34_K37_A1.7731.00
182_L185_K1.7671.00
78_Y92_A1.7481.00
28_Q65_E1.7271.00
191_T194_R1.7171.00
130_F145_V1.7121.00
189_Q233_A1.7061.00
144_S152_E1.7021.00
169_T239_F1.6981.00
220_N223_R1.6681.00
70_A98_L1.6671.00
112_M134_L1.6611.00
278_K281_E1.6291.00
75_A313_F1.6221.00
162_A243_V1.6131.00
255_L288_G1.5981.00
5_G309_G1.5971.00
63_L70_A1.5911.00
70_A73_I1.5821.00
27_N296_E1.5651.00
186_M233_A1.5601.00
144_S258_T1.5571.00
158_I208_D1.5551.00
74_D104_V1.5451.00
283_M290_V1.5411.00
147_G150_Q1.5281.00
248_M278_K1.5131.00
186_M232_R1.5121.00
20_D310_M1.5071.00
113_E151_Y1.4901.00
28_Q58_L1.4821.00
252_K282_M1.4641.00
237_R241_D1.4561.00
274_T278_K1.4061.00
26_A62_A1.4031.00
265_M292_Y1.4031.00
95_G106_A1.4021.00
172_L219_A1.3921.00
184_S240_E1.3801.00
225_N228_D1.3711.00
199_R242_A1.3581.00
30_Y54_K1.3561.00
312_R317_A1.3531.00
228_D231_T1.3491.00
108_P319_A1.3441.00
181_P185_K1.3331.00
135_V275_L1.3321.00
6_I91_G1.3321.00
241_D278_K1.3231.00
277_A281_E1.3131.00
14_G302_G1.3101.00
198_P217_F1.2911.00
97_S101_A1.2871.00
218_A239_F1.2851.00
128_F148_I1.2851.00
24_L297_F1.2841.00
197_F235_I1.2841.00
194_R234_D1.2811.00
142_L251_C1.2791.00
3_V77_A1.2781.00
214_L243_V1.2721.00
69_T72_D1.2661.00
154_L258_T1.2611.00
244_V278_K1.2571.00
19_D25_L1.2571.00
244_V275_L1.2531.00
185_K189_Q1.2491.00
197_F217_F1.2461.00
5_G302_G1.2431.00
2_R17_I1.2431.00
3_V313_F1.2311.00
147_G315_A1.2281.00
17_I62_A1.2281.00
27_N62_A1.2271.00
14_G29_L1.2201.00
274_T277_A1.2141.00
26_A66_S1.2041.00
17_I26_A1.2031.00
307_Y311_V1.1930.99
262_R291_F1.1870.99
185_K188_A1.1860.99
119_P264_V1.1680.99
271_A295_P1.1610.99
130_F264_V1.1490.99
281_E285_K1.1480.99
240_E274_T1.1400.99
28_Q62_A1.1360.99
120_M311_V1.1270.99
186_M189_Q1.1190.99
12_E33_V1.1140.99
182_L186_M1.1140.99
193_G241_D1.1110.99
251_C279_L1.1000.99
172_L222_I1.0940.99
142_L209_F1.0930.99
62_A66_S1.0900.99
282_M285_K1.0850.99
160_D215_K1.0840.99
252_K286_R1.0840.99
222_I235_I1.0820.99
205_P253_R1.0820.99
184_S188_A1.0790.99
78_Y95_G1.0790.99
218_A235_I1.0770.99
282_M286_R1.0760.99
220_N224_D1.0740.99
21_E310_M1.0730.99
75_A107_I1.0720.99
131_V258_T1.0640.99
85_V325_V1.0640.99
191_T234_D1.0630.99
80_A108_P1.0610.99
68_L72_D1.0570.99
237_R240_E1.0560.99
17_I73_I1.0540.99
76_V99_A1.0500.99
116_L132_A1.0470.99
191_T237_R1.0470.99
313_F318_T1.0430.99
20_D314_K1.0420.99
6_I59_I1.0390.99
107_I318_T1.0360.99
155_G254_A1.0290.99
94_V97_S1.0160.98
63_L68_L1.0140.98
92_A325_V1.0130.98
199_R249_I1.0050.98
85_V327_P1.0030.98
35_L50_D1.0030.98
194_R231_T1.0000.98
27_N303_A0.9930.98
195_F234_D0.9900.98
248_M282_M0.9880.98
98_L102_W0.9860.98
221_T225_N0.9810.98
199_R246_T0.9790.98
55_T94_V0.9770.98
278_K282_M0.9750.98
311_V314_K0.9740.98
232_R236_A0.9690.98
319_A323_V0.9680.98
71_K102_W0.9670.98
169_T172_L0.9670.98
153_L156_E0.9580.98
16_A302_G0.9560.98
245_D278_K0.9550.98
186_M236_A0.9540.98
2_R74_D0.9530.98
73_I102_W0.9500.97
141_Q156_E0.9500.97
127_E262_R0.9440.97
106_A323_V0.9430.97
25_L68_L0.9370.97
81_G110_H0.9360.97
15_I62_A0.9280.97
202_T249_I0.9260.97
252_K285_K0.9260.97
194_R238_A0.9190.97
184_S274_T0.9170.97
265_M279_L0.9170.97
314_K317_A0.9160.97
173_L222_I0.9130.97
17_I68_L0.9120.97
18_Y307_Y0.9100.97
14_G27_N0.9080.97
294_R297_F0.9040.97
108_P323_V0.9010.97
188_A277_A0.9000.97
249_I253_R0.8990.96
166_F184_S0.8990.96
190_G237_R0.8940.96
117_L148_I0.8890.96
119_P266_A0.8870.96
252_K256_D0.8830.96
241_D245_D0.8780.96
113_E149_G0.8770.96
162_A247_L0.8740.96
227_T230_Q0.8700.96
126_P130_F0.8690.96
80_A326_R0.8650.96
123_D289_E0.8560.95
242_A246_T0.8540.95
76_V104_V0.8500.95
76_V102_W0.8480.95
326_R329_W0.8470.95
92_A96_R0.8450.95
203_D206_G0.8440.95
280_A290_V0.8410.95
263_L283_M0.8410.95
107_I313_F0.8390.95
117_L307_Y0.8380.95
120_M307_Y0.8360.95
93_T97_S0.8330.95
56_V94_V0.8320.95
113_E312_R0.8320.95
234_D238_A0.8320.95
207_L253_R0.8260.94
225_N231_T0.8260.94
15_I28_Q0.8220.94
64_K67_G0.8200.94
223_R226_G0.8190.94
165_A239_F0.8160.94
143_I153_L0.8130.94
173_L236_A0.8120.94
131_V260_F0.8080.94
199_R241_D0.8080.94
277_A280_A0.8060.94
162_A211_F0.8050.94
117_L308_A0.8010.93
18_Y24_L0.8000.93
15_I302_G0.7990.93
283_M288_G0.7980.93
12_E87_A0.7960.93
99_A104_V0.7930.93
270_S298_C0.7920.93
206_G257_Q0.7910.93
217_F221_T0.7870.93
252_K255_L0.7850.93
173_L232_R0.7830.93
280_A292_Y0.7810.93
48_S86_G0.7810.93
271_A299_T0.7780.92
24_L303_A0.7770.92
45_E49_R0.7760.92
202_T206_G0.7700.92
234_D237_R0.7680.92
169_T173_L0.7660.92
231_T234_D0.7660.92
45_E86_G0.7660.92
12_E177_Y0.7650.92
135_V247_L0.7650.92
143_I151_Y0.7630.92
164_E215_K0.7620.92
117_L130_F0.7610.92
98_L104_V0.7570.91
192_A241_D0.7550.91
166_F240_E0.7550.91
170_A176_D0.7530.91
206_G253_R0.7520.91
116_L134_L0.7510.91
248_M251_C0.7490.91
261_K289_E0.7480.91
255_L258_T0.7470.91
201_M206_G0.7460.91
132_A266_A0.7440.91
211_F243_V0.7440.91
8_T13_T0.7400.90
131_V255_L0.7370.90
121_L303_A0.7340.90
18_Y306_A0.7300.90
34_K38_D0.7280.90
25_L62_A0.7280.90
68_L73_I0.7280.90
55_T90_V0.7270.90
246_T250_K0.7260.90
75_A108_P0.7250.90
312_R318_T0.7250.90
27_N297_F0.7230.89
117_L151_Y0.7220.89
162_A214_L0.7220.89
92_A323_V0.7210.89
166_F239_F0.7180.89
85_V88_L0.7120.89
141_Q153_L0.7090.88
309_G313_F0.7070.88
32_Q36_H0.7050.88
175_L186_M0.7030.88
190_G195_F0.7010.88
61_A64_K0.7010.88
96_R106_A0.7000.88
171_K236_A0.6990.88
12_E31_S0.6990.88
255_L282_M0.6940.87
92_A106_A0.6910.87
2_R73_I0.6910.87
133_L251_C0.6900.87
89_L325_V0.6870.87
76_V95_G0.6840.87
281_E284_K0.6830.87
248_M279_L0.6790.86
8_T91_G0.6780.86
118_A266_A0.6770.86
252_K283_M0.6760.86
20_D23_G0.6750.86
199_R202_T0.6730.86
26_A65_E0.6690.85
190_G238_A0.6680.85
18_Y75_A0.6630.85
26_A63_L0.6600.85
252_K288_G0.6600.85
10_C42_V0.6570.84
30_Y61_A0.6540.84
6_I15_I0.6540.84
155_G208_D0.6540.84
279_L290_V0.6480.84
123_D291_F0.6470.83
195_F231_T0.6470.83
244_V274_T0.6460.83
80_A323_V0.6460.83
166_F183_L0.6460.83
202_T246_T0.6440.83
29_L296_E0.6410.83
78_Y323_V0.6410.83
201_M208_D0.6380.83
39_Y46_L0.6360.82
184_S244_V0.6360.82
326_R330_P0.6360.82
134_L141_Q0.6350.82
59_I98_L0.6350.82
40_G168_K0.6340.82
71_K104_V0.6310.82
128_F145_V0.6310.82
80_A329_W0.6290.82
81_G329_W0.6270.82
130_F148_I0.6260.81
75_A309_G0.6240.81
165_A215_K0.6200.81
192_A237_R0.6200.81
109_V308_A0.6190.81
217_F220_N0.6190.81
24_L27_N0.6190.81
160_D164_E0.6190.81
132_A264_V0.6150.80
30_Y58_L0.6140.80
195_F225_N0.6140.80
6_I55_T0.6080.80
87_A110_H0.6060.79
245_D249_I0.6040.79
18_Y21_E0.6040.79
270_S294_R0.6030.79
12_E29_L0.6030.79
70_A104_V0.6020.79
15_I26_A0.6010.79
164_E168_K0.5990.79
126_P291_F0.5980.78
159_D210_S0.5980.78
158_I201_M0.5960.78
201_M250_K0.5940.78
117_L120_M0.5900.77
2_R5_G0.5900.77
307_Y310_M0.5890.77
94_V98_L0.5890.77
45_E48_S0.5890.77
154_L257_Q0.5870.77
318_T322_G0.5870.77
216_T220_N0.5870.77
276_R292_Y0.5830.77
253_R257_Q0.5830.77
242_A245_D0.5820.77
283_M286_R0.5810.76
225_N230_Q0.5810.76
78_Y91_G0.5800.76
80_A92_A0.5780.76
193_G238_A0.5770.76
207_L258_T0.5760.76
3_V309_G0.5750.76
42_V177_Y0.5730.75
169_T243_V0.5730.75
24_L306_A0.5720.75
221_T224_D0.5720.75
48_S90_V0.5720.75
63_L102_W0.5720.75
196_V238_A0.5700.75
119_P265_M0.5700.75
123_D126_P0.5690.75
123_D294_R0.5690.75
198_P242_A0.5680.75
240_E272_N0.5680.75
84_L178_P0.5670.75
127_E289_E0.5660.75
2_R25_L0.5640.74
120_M128_F0.5640.74
152_E258_T0.5620.74
56_V302_G0.5620.74
174_G232_R0.5610.74
230_Q233_A0.5610.74
110_H113_E0.5600.74
54_K57_P0.5600.74
37_A176_D0.5590.74
263_L290_V0.5560.73
155_G207_L0.5560.73
312_R319_A0.5560.73
76_V106_A0.5530.73
238_A241_D0.5520.73
131_V254_A0.5510.73
8_T55_T0.5490.72
230_Q234_D0.5490.72
148_I311_V0.5480.72
229_D232_R0.5460.72
134_L139_H0.5460.72
112_M141_Q0.5460.72
19_D310_M0.5440.72
97_S102_W0.5430.71
221_T235_I0.5430.71
170_A299_T0.5430.71
122_E297_F0.5420.71
197_F214_L0.5400.71
17_I66_S0.5390.71
251_C255_L0.5390.71
100_F321_L0.5380.71
103_D281_E0.5360.71
68_L102_W0.5360.71
128_F147_G0.5340.70
130_F262_R0.5320.70
126_P262_R0.5310.70
297_F307_Y0.5310.70
255_L259_G0.5300.70
15_I94_V0.5290.70
4_L15_I0.5280.69
142_L254_A0.5280.69
117_L145_V0.5270.69
17_I63_L0.5270.69
131_V251_C0.5260.69
49_R178_P0.5250.69
29_L299_T0.5250.69
74_D318_T0.5240.69
222_I226_G0.5220.69
4_L17_I0.5200.68
312_R316_G0.5190.68
131_V263_L0.5180.68
89_L93_T0.5180.68
35_L54_K0.5180.68
116_L143_I0.5170.68
24_L121_L0.5170.68
117_L128_F0.5160.68
9_S302_G0.5160.68
317_A320_D0.5150.68
160_D165_A0.5130.67
8_T139_H0.5120.67
84_L215_K0.5110.67
84_L164_E0.5110.67
5_G112_M0.5110.67
120_M148_I0.5110.67
121_L264_V0.5100.67
142_L263_L0.5080.66
12_E52_V0.5080.66
215_K219_A0.5070.66
36_H47_A0.5050.66
244_V248_M0.5040.66
8_T30_Y0.5030.66
151_Y312_R0.5030.66
25_L66_S0.5020.66
261_K288_G0.5020.66
218_A238_A0.5020.66
63_L69_T0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3en9A 1 0.9525 100 0.335 Contact Map
4k25A 2 0.9466 100 0.365 Contact Map
3zeuB 1 1 100 0.398 Contact Map
2ivnA 1 0.9555 100 0.442 Contact Map
3enoA 1 0.9555 100 0.455 Contact Map
3venA 1 0.9347 100 0.48 Contact Map
3vthA 1 0.8398 100 0.59 Contact Map
4g9iA 1 0.8665 100 0.62 Contact Map
3ttcA 1 0.822 100 0.622 Contact Map
4m7xA 2 0.7715 100 0.671 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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