GREMLIN Database
T1SK - Type-1 restriction enzyme EcoKI specificity protein
UniProt: P05719 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10460
Length: 464 (385)
Sequences: 2063 (2052)
Seq/√Len: 104.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
293_Y359_G4.3671.00
319_K359_G4.1191.00
77_D112_R4.0211.00
293_Y319_K4.0011.00
107_F146_N3.4761.00
83_S107_F3.4351.00
83_S146_N3.2401.00
292_R318_D3.1541.00
84_S145_N2.9691.00
118_F166_K2.8841.00
287_D324_R2.8681.00
416_K421_E2.8091.00
107_F144_I2.6811.00
294_N358_K2.6801.00
82_M107_F2.6731.00
293_Y357_Q2.6481.00
332_E386_E2.6371.00
74_S101_E2.6341.00
292_R319_K2.5371.00
82_M146_N2.4851.00
237_N240_S2.4231.00
292_R321_I2.3261.00
284_Q324_R2.2321.00
104_F110_V2.2311.00
319_K357_Q2.2301.00
325_L334_I2.1821.00
75_P101_E2.1801.00
149_P153_D2.1501.00
84_S89_V2.0961.00
232_L323_A2.0951.00
88_S144_I2.0881.00
292_R317_P2.0691.00
316_Y322_R2.0601.00
330_L379_A2.0561.00
240_S318_D2.0101.00
83_S106_A1.9941.00
82_M109_G1.9931.00
292_R359_G1.9381.00
255_I293_Y1.9311.00
294_N356_G1.8611.00
293_Y318_D1.8151.00
46_A81_A1.7961.00
83_S144_I1.7861.00
321_I362_G1.7851.00
226_L372_L1.7631.00
108_C146_N1.7071.00
14_P154_L1.6791.00
353_T358_K1.6741.00
82_M106_A1.6531.00
293_Y317_P1.5861.00
254_R317_P1.5841.00
296_S300_V1.5731.00
82_M108_C1.5691.00
237_N319_K1.5421.00
240_S319_K1.5411.00
95_H120_G1.5381.00
316_Y319_K1.5151.00
14_P156_N1.5011.00
229_E366_K1.4931.00
83_S109_G1.4921.00
84_S144_I1.4831.00
254_R318_D1.4741.00
294_N357_Q1.4741.00
255_I319_K1.4671.00
186_F395_I1.4621.00
237_N317_P1.4581.00
84_S88_S1.4551.00
74_S77_D1.4511.00
83_S89_V1.4341.00
46_A83_S1.4321.00
255_I294_N1.4321.00
184_A187_E1.4291.00
237_N293_Y1.4241.00
255_I291_T1.4211.00
254_R319_K1.4161.00
13_A159_I1.4091.00
375_V378_Q1.4001.00
84_S107_F1.3981.00
240_S317_P1.3971.00
23_R83_S1.3861.00
212_K215_N1.3781.00
285_D307_K1.3731.00
105_G108_C1.3671.00
46_A82_M1.3651.00
294_N319_K1.3611.00
294_N317_P1.3550.99
89_V107_F1.3470.99
104_F108_C1.3450.99
188_Q192_I1.3390.99
417_A422_L1.3370.99
362_G366_K1.3360.99
81_A89_V1.3350.99
89_V144_I1.3340.99
329_A334_I1.3300.99
81_A144_I1.3300.99
281_H315_L1.3240.99
182_T185_R1.3210.99
117_I122_I1.3200.99
45_R146_N1.3120.99
296_S319_K1.3080.99
79_V108_C1.2970.99
294_N301_G1.2950.99
226_L230_S1.2920.99
289_L320_L1.2910.99
29_K60_V1.2830.99
108_C144_I1.2790.99
220_H223_F1.2720.99
104_F107_F1.2690.99
96_Q102_C1.2650.99
105_G144_I1.2630.99
82_M105_G1.2620.99
23_R106_A1.2620.99
291_T296_S1.2600.99
109_G152_F1.2510.99
318_D359_G1.2470.99
252_I317_P1.2460.99
296_S356_G1.2430.99
71_Q107_F1.2370.99
409_L412_S1.2370.99
281_H322_R1.2340.99
237_N292_R1.2270.99
118_F169_A1.2270.99
237_N322_R1.2190.99
291_T302_V1.2150.99
239_L319_K1.2140.99
321_I365_I1.2100.99
90_V144_I1.2090.99
45_R107_F1.2080.99
193_L197_R1.2040.99
292_R316_Y1.2020.99
120_G173_D1.1980.99
82_M144_I1.1980.99
317_P359_G1.1950.99
255_I317_P1.1940.99
317_P320_L1.1930.99
305_L335_E1.1920.99
254_R296_S1.1900.99
318_D321_I1.1890.99
293_Y316_Y1.1740.99
210_T213_W1.1700.99
193_L196_F1.1690.98
81_A92_K1.1690.98
46_A107_F1.1690.98
26_T105_G1.1680.98
169_A173_D1.1630.98
346_A369_V1.1630.98
19_T122_I1.1590.98
239_L318_D1.1570.98
188_Q405_R1.1550.98
73_I104_F1.1520.98
213_W216_F1.1490.98
26_T106_A1.1450.98
281_H319_K1.1440.98
241_S318_D1.1440.98
252_I319_K1.1430.98
377_E381_I1.1360.98
83_S143_N1.1320.98
381_I385_V1.1300.98
182_T189_I1.1290.98
294_N300_V1.1260.98
175_L178_Q1.1230.98
83_S105_G1.1200.98
241_S319_K1.1160.98
255_I359_G1.1100.98
207_G210_T1.1080.98
77_D110_V1.1050.98
45_R83_S1.1010.98
45_R105_G1.0990.98
74_S112_R1.0940.98
187_E191_Q1.0890.98
178_Q182_T1.0870.98
89_V92_K1.0860.98
45_R106_A1.0850.98
291_T356_G1.0820.97
46_A146_N1.0800.97
45_R48_N1.0750.97
23_R105_G1.0720.97
206_N209_L1.0710.97
292_R362_G1.0660.97
46_A105_G1.0590.97
161_P164_E1.0580.97
283_L314_L1.0550.97
223_F373_P1.0530.97
27_Y107_F1.0530.97
246_S249_G1.0460.97
109_G146_N1.0460.97
340_S343_A1.0450.97
293_Y321_I1.0450.97
89_V148_K1.0370.97
14_P17_T1.0350.97
255_I357_Q1.0350.97
84_S146_N1.0280.97
240_S293_Y1.0270.97
289_L306_L1.0270.97
236_R281_H1.0270.97
83_S104_F1.0250.97
291_T357_Q1.0250.97
169_A172_L1.0230.96
316_Y320_L1.0220.96
240_S359_G1.0210.96
84_S143_N1.0180.96
109_G149_P1.0170.96
356_G359_G1.0150.96
12_I156_N1.0150.96
45_R82_M1.0140.96
229_E367_S1.0130.96
254_R359_G1.0030.96
89_V105_G1.0030.96
106_A146_N1.0010.96
185_R189_I1.0010.96
182_T197_R0.9980.96
186_F196_F0.9980.96
143_N146_N0.9970.96
80_I135_I0.9930.96
263_V304_G0.9930.96
203_G219_Q0.9920.96
375_V379_A0.9860.96
412_S416_K0.9850.96
16_S153_D0.9830.96
25_V71_Q0.9820.96
107_F143_N0.9820.96
82_M143_N0.9810.96
178_Q186_F0.9750.95
343_A347_M0.9750.95
79_V96_Q0.9740.95
105_G109_G0.9690.95
81_A107_F0.9600.95
240_S252_I0.9570.95
89_V145_N0.9550.95
106_A109_G0.9530.95
82_M90_V0.9460.95
82_M103_S0.9390.94
294_N318_D0.9380.94
123_A127_K0.9360.94
185_R188_Q0.9350.94
292_R320_L0.9330.94
84_S105_G0.9310.94
188_Q191_Q0.9290.94
81_A84_S0.9280.94
338_F343_A0.9270.94
166_K170_E0.9260.94
84_S90_V0.9220.94
83_S90_V0.9200.94
292_R357_Q0.9180.94
293_Y296_S0.9180.94
239_L293_Y0.9140.94
292_R361_S0.9130.93
47_N83_S0.9120.93
164_E167_I0.9120.93
414_L418_F0.9110.93
227_N367_S0.9090.93
237_N318_D0.9070.93
23_R82_M0.9020.93
46_A108_C0.8990.93
241_S317_P0.8990.93
107_F148_K0.8980.93
240_S292_R0.8960.93
291_T358_K0.8950.93
197_R202_G0.8950.93
197_R200_V0.8950.93
43_L83_S0.8930.93
105_G110_V0.8900.93
237_N362_G0.8890.93
238_G293_Y0.8860.92
19_T111_L0.8840.92
26_T43_L0.8840.92
186_F192_I0.8800.92
88_S91_G0.8780.92
73_I146_N0.8730.92
418_F422_L0.8710.92
380_E383_R0.8670.92
291_T318_D0.8660.92
83_S92_K0.8650.91
227_N230_S0.8640.91
46_A50_Q0.8620.91
254_R357_Q0.8620.91
179_V410_T0.8580.91
320_L359_G0.8580.91
104_F146_N0.8550.91
252_I318_D0.8530.91
25_V107_F0.8530.91
241_S292_R0.8520.91
330_L382_V0.8520.91
21_L24_G0.8510.91
109_G144_I0.8470.91
255_I281_H0.8470.91
212_K216_F0.8410.90
27_Y43_L0.8410.90
46_A106_A0.8390.90
416_K422_L0.8390.90
255_I358_K0.8390.90
43_L106_A0.8380.90
12_I158_P0.8380.90
359_G362_G0.8370.90
317_P357_Q0.8350.90
316_Y321_I0.8340.90
252_I315_L0.8300.90
294_N344_R0.8300.90
84_S108_C0.8280.90
82_M104_F0.8280.90
23_R107_F0.8270.90
162_L166_K0.8270.90
83_S96_Q0.8210.89
258_V304_G0.8210.89
319_K356_G0.8180.89
90_V132_R0.8180.89
293_Y356_G0.8160.89
77_D102_C0.8150.89
240_S357_Q0.8130.89
90_V107_F0.8100.89
252_I292_R0.8100.89
45_R60_V0.8080.88
303_C335_E0.8080.88
237_N359_G0.8070.88
240_S255_I0.8050.88
186_F189_I0.8040.88
197_R203_G0.7990.88
24_G106_A0.7980.88
291_T294_N0.7960.88
281_H292_R0.7960.88
173_D176_L0.7940.88
106_A144_I0.7860.87
25_V106_A0.7840.87
287_D322_R0.7840.87
90_V139_S0.7830.87
46_A109_G0.7800.87
20_T112_R0.7800.87
240_S316_Y0.7770.86
83_S147_I0.7730.86
83_S108_C0.7690.86
28_K106_A0.7660.86
83_S141_G0.7660.86
275_E315_L0.7660.86
254_R292_R0.7650.86
317_P321_I0.7650.86
124_H172_L0.7610.85
240_S243_P0.7580.85
146_N149_P0.7570.85
83_S149_P0.7560.85
22_I112_R0.7550.85
240_S281_H0.7550.85
207_G215_N0.7550.85
237_N243_P0.7530.85
291_T319_K0.7500.84
243_P293_Y0.7490.84
139_S146_N0.7480.84
88_S143_N0.7480.84
194_K203_G0.7470.84
196_F202_G0.7450.84
90_V143_N0.7450.84
320_L357_Q0.7430.84
92_K146_N0.7430.84
255_I296_S0.7390.84
236_R318_D0.7360.83
21_L149_P0.7340.83
49_I81_A0.7300.83
13_A17_T0.7280.83
240_S315_L0.7260.83
26_T82_M0.7260.83
64_K68_K0.7260.83
193_L200_V0.7240.82
256_S294_N0.7240.82
253_L320_L0.7230.82
290_F357_Q0.7180.82
257_S267_D0.7180.82
211_E215_N0.7180.82
239_L317_P0.7170.82
103_S106_A0.7170.82
254_R269_R0.7170.82
259_R302_V0.7160.82
103_S107_F0.7150.82
24_G27_Y0.7140.81
321_I357_Q0.7130.81
109_G148_K0.7110.81
82_M110_V0.7110.81
411_Q415_A0.7100.81
70_S103_S0.7100.81
255_I320_L0.7100.81
281_H357_Q0.7050.81
108_C152_F0.7050.81
81_A146_N0.7040.81
293_Y320_L0.7030.80
256_S293_Y0.7010.80
89_V139_S0.7010.80
175_L409_L0.7000.80
284_Q287_D0.6990.80
405_R409_L0.6990.80
82_M89_V0.6990.80
23_R109_G0.6970.80
197_R201_L0.6940.80
202_G415_A0.6920.79
23_R43_L0.6910.79
164_E423_T0.6910.79
294_N316_Y0.6900.79
239_L281_H0.6890.79
240_S294_N0.6890.79
107_F145_N0.6870.79
143_N148_K0.6870.79
44_I102_C0.6860.79
23_R149_P0.6860.79
239_L321_I0.6850.79
236_R322_R0.6850.79
321_I359_G0.6830.79
80_I84_S0.6820.78
107_F110_V0.6810.78
27_Y103_S0.6790.78
176_L179_V0.6790.78
23_R27_Y0.6790.78
42_P84_S0.6760.78
192_I196_F0.6740.78
220_H225_K0.6720.77
89_V146_N0.6710.77
84_S92_K0.6710.77
181_S185_R0.6700.77
186_F200_V0.6690.77
46_A89_V0.6690.77
227_N369_V0.6680.77
43_L105_G0.6670.77
237_N316_Y0.6670.77
23_R26_T0.6660.77
182_T395_I0.6660.77
180_D184_A0.6650.77
180_D183_K0.6650.77
193_L198_Q0.6640.77
254_R281_H0.6620.76
318_D357_Q0.6620.76
182_T410_T0.6590.76
88_S145_N0.6580.76
27_Y106_A0.6570.76
71_Q84_S0.6570.76
237_N281_H0.6560.76
374_P377_E0.6560.76
241_S293_Y0.6530.75
237_N321_I0.6530.75
300_V360_I0.6530.75
271_L294_N0.6520.75
82_M88_S0.6510.75
105_G146_N0.6490.75
81_A108_C0.6450.74
166_K169_A0.6410.74
257_S317_P0.6410.74
42_P46_A0.6400.74
211_E216_F0.6400.74
46_A144_I0.6380.74
178_Q391_Y0.6380.74
136_S145_N0.6340.73
82_M148_K0.6320.73
197_R410_T0.6320.73
238_G241_S0.6300.73
294_N302_V0.6280.73
255_I292_R0.6250.72
402_A410_T0.6250.72
181_S394_T0.6220.72
316_Y359_G0.6200.72
257_S294_N0.6190.71
23_R146_N0.6190.71
134_K137_S0.6180.71
252_I359_G0.6170.71
208_K221_S0.6160.71
239_L292_R0.6160.71
15_V126_T0.6150.71
243_P315_L0.6150.71
199_A412_S0.6130.71
92_K107_F0.6130.71
104_F139_S0.6130.71
196_F199_A0.6130.71
117_I159_I0.6120.71
84_S132_R0.6090.70
283_L316_Y0.6090.70
107_F147_I0.6090.70
46_A84_S0.6080.70
94_A144_I0.6060.70
196_F409_L0.6050.70
120_G124_H0.6050.70
255_I316_Y0.6000.69
255_I259_R0.5990.69
50_Q106_A0.5990.69
314_L319_K0.5980.69
325_L331_P0.5980.69
10_W164_E0.5950.68
92_K104_F0.5950.68
89_V106_A0.5920.68
251_P255_I0.5910.68
241_S316_Y0.5890.68
84_S106_A0.5850.67
23_R29_K0.5810.67
185_R196_F0.5810.67
43_L46_A0.5790.66
178_Q199_A0.5790.66
353_T359_G0.5770.66
301_G358_K0.5770.66
170_E174_T0.5740.66
69_E83_S0.5740.66
254_R257_S0.5730.66
198_Q203_G0.5730.66
182_T403_L0.5720.65
73_I82_M0.5720.65
191_Q194_K0.5700.65
407_N411_Q0.5680.65
291_T300_V0.5670.65
25_V70_S0.5650.64
241_S315_L0.5650.64
201_L381_I0.5650.64
77_D104_F0.5650.64
292_R365_I0.5640.64
183_K407_N0.5610.64
190_P195_R0.5600.64
347_M351_V0.5590.64
26_T107_F0.5580.64
90_V105_G0.5580.64
45_R84_S0.5570.63
186_F190_P0.5570.63
412_S418_F0.5570.63
300_V317_P0.5560.63
88_S106_A0.5560.63
93_S123_A0.5550.63
241_S321_I0.5550.63
256_S319_K0.5540.63
223_F374_P0.5540.63
178_Q402_A0.5530.63
255_I318_D0.5520.63
292_R314_L0.5510.63
23_R71_Q0.5480.62
83_S145_N0.5460.62
192_I195_R0.5450.62
300_V356_G0.5440.62
26_T103_S0.5440.62
81_A88_S0.5440.62
350_C364_D0.5440.62
70_S81_A0.5430.61
239_L359_G0.5420.61
89_V109_G0.5420.61
29_K82_M0.5400.61
171_K413_I0.5390.61
281_H359_G0.5390.61
83_S139_S0.5390.61
76_E119_S0.5380.61
178_Q189_I0.5380.61
182_T202_G0.5360.60
73_I110_V0.5360.60
351_V358_K0.5360.60
192_I405_R0.5350.60
107_F149_P0.5320.60
319_K362_G0.5310.60
195_R412_S0.5290.59
240_S321_I0.5290.59
415_A418_F0.5270.59
194_K198_Q0.5270.59
196_F200_V0.5240.59
45_R145_N0.5240.59
92_K139_S0.5230.59
241_S277_E0.5230.59
70_S108_C0.5200.58
113_P119_S0.5180.58
27_Y105_G0.5170.58
237_N255_I0.5170.58
47_N144_I0.5170.58
106_A147_I0.5170.58
81_A90_V0.5150.57
45_R89_V0.5130.57
190_P196_F0.5130.57
89_V143_N0.5110.57
283_L322_R0.5100.57
255_I300_V0.5100.57
43_L48_N0.5100.57
96_Q100_F0.5100.57
346_A350_C0.5090.57
254_R267_D0.5080.56
21_L109_G0.5060.56
252_I294_N0.5060.56
319_K353_T0.5060.56
43_L82_M0.5050.56
171_K189_I0.5050.56
283_L293_Y0.5040.56
71_Q82_M0.5040.56
314_L317_P0.5010.55
27_Y82_M0.5010.55
278_L281_H0.5000.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2y7cA 1 0.9828 100 0.215 Contact Map
1yf2A 1 0.8621 100 0.387 Contact Map
3okgA 2 0.8082 100 0.408 Contact Map
1ydxA 1 0.7737 100 0.433 Contact Map
2ih2A 1 0.3276 93.6 0.943 Contact Map
3s1sA 1 0.4612 59.4 0.961 Contact Map
3h4pa 5 0.4073 13.6 0.972 Contact Map
2r9iA 1 0.1379 13.3 0.973 Contact Map
1owxA 1 0.2435 11.8 0.973 Contact Map
2xvsA 1 0.3147 11.7 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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