GREMLIN Database
ALKB - Alpha-ketoglutarate-dependent dioxygenase AlkB
UniProt: P05050 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10037
Length: 216 (199)
Sequences: 1263 (803)
Seq/√Len: 56.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
150_L195_G5.1511.00
36_D95_S4.1151.00
41_A62_T3.4151.00
18_G181_E3.0161.00
160_K163_D2.9021.00
115_D212_A2.8841.00
63_N116_A2.8771.00
16_A182_S2.7881.00
29_A102_R2.4531.00
103_A144_V2.4281.00
17_A181_E2.2771.00
18_G182_S2.2701.00
19_A156_F2.2191.00
19_A176_V2.2131.00
70_T79_S2.1841.00
146_V175_V2.1741.00
182_S185_F2.1661.00
169_L193_K2.1571.00
30_A172_H2.1421.00
134_K186_Y2.0291.00
92_M97_H1.9831.00
101_Q113_Q1.9421.00
72_H77_L1.9391.00
22_L175_V1.8741.00
152_A189_I1.8370.99
159_L185_F1.8330.99
44_S85_T1.8250.99
78_Y83_P1.8210.99
49_M71_T1.8190.99
100_C209_F1.8080.99
37_I205_Y1.8040.99
26_A146_V1.7950.99
155_Q190_Q1.7830.99
200_T203_C1.7500.99
48_Q51_T1.7460.99
96_F209_F1.7020.99
156_F185_F1.6980.99
55_Y129_S1.6340.99
98_N102_R1.6190.99
16_A156_F1.6150.99
94_Q98_N1.6100.99
30_A200_T1.6090.99
156_F176_V1.5980.99
151_P171_E1.5720.98
41_A46_F1.5500.98
103_A175_V1.5390.98
200_T205_Y1.5380.98
121_R203_C1.5360.98
99_L146_V1.5260.98
158_G163_D1.5180.98
59_V128_L1.5030.98
150_L196_F1.4690.97
19_A182_S1.4380.97
62_T119_I1.4080.97
72_H78_Y1.3700.96
155_Q165_L1.3650.96
77_L83_P1.3650.96
46_F121_R1.3610.96
97_H101_Q1.3500.96
194_A202_D1.3400.96
36_D39_D1.3290.95
145_S206_N1.3190.95
57_M71_T1.3050.95
130_L188_G1.3030.95
144_V177_V1.3020.95
36_D40_V1.2890.94
23_R171_E1.2840.94
98_N119_I1.2790.94
72_H83_P1.2720.94
45_P116_A1.2710.94
50_V53_G1.2080.92
97_H113_Q1.2080.92
44_S90_P1.2030.92
16_A19_A1.1720.91
75_G78_Y1.1550.90
48_Q60_A1.1410.90
68_G83_P1.1380.90
204_R210_R1.1290.89
40_V64_C1.1280.89
68_G78_Y1.1260.89
92_M209_F1.1130.89
144_V179_G1.1070.88
73_R78_Y1.0890.88
132_Q186_Y1.0670.87
50_V56_T1.0660.87
21_I168_L1.0520.86
18_G30_A1.0510.86
197_H200_T1.0500.86
197_H201_I1.0380.85
32_Q36_D1.0310.85
25_F103_A1.0300.85
79_S128_L1.0220.84
34_I38_N1.0220.84
15_L19_A1.0210.84
104_A108_G1.0090.83
153_I167_R0.9990.83
15_L26_A0.9890.82
30_A37_I0.9870.82
38_N41_A0.9870.82
151_P169_L0.9650.80
47_R82_D0.9570.80
16_A21_I0.9550.80
74_Q78_Y0.9540.80
34_I199_L0.9360.78
95_S98_N0.9340.78
165_L192_L0.9340.78
157_G161_R0.9320.78
15_L156_F0.9320.78
33_L96_F0.9300.78
145_S174_D0.9170.77
158_G161_R0.9080.76
33_L148_L0.9080.76
161_R186_Y0.9030.76
25_F106_A0.9000.76
178_W208_T0.8920.75
34_I198_P0.8900.75
150_L197_H0.8800.74
48_Q56_T0.8720.73
32_Q98_N0.8720.73
99_L186_Y0.8720.73
55_Y73_R0.8680.73
105_T111_D0.8650.73
47_R52_P0.8630.73
46_F49_M0.8610.72
196_F200_T0.8590.72
115_D137_P0.8520.72
37_I200_T0.8470.71
47_R51_T0.8440.71
67_L116_A0.8430.71
96_F178_W0.8390.70
117_C207_L0.8310.70
172_H197_H0.8250.69
139_L189_I0.8230.69
63_N143_I0.8210.69
16_A185_F0.8150.68
169_L190_Q0.8120.68
69_W76_Y0.8110.68
142_P177_V0.8100.68
29_A32_Q0.8090.68
51_T54_G0.8050.67
153_I169_L0.7980.67
155_Q167_R0.7960.66
183_R187_H0.7930.66
124_P150_L0.7930.66
134_K184_L0.7900.66
61_M182_S0.7880.66
148_L157_G0.7850.65
77_L80_P0.7840.65
124_P194_A0.7790.65
70_T77_L0.7780.65
13_E23_R0.7770.64
68_G143_I0.7750.64
74_Q79_S0.7740.64
169_L185_F0.7730.64
91_A101_Q0.7610.63
155_Q191_P0.7600.63
63_N68_G0.7580.63
39_D98_N0.7560.62
46_F51_T0.7510.62
35_R39_D0.7500.62
206_N210_R0.7490.62
40_V96_F0.7470.61
153_I193_K0.7450.61
51_T55_Y0.7440.61
162_N165_L0.7430.61
55_Y128_L0.7390.61
123_A194_A0.7360.60
103_A177_V0.7330.60
33_L99_L0.7300.60
171_E181_E0.7270.59
41_A200_T0.7220.59
92_M95_S0.7210.59
99_L102_R0.7200.59
94_Q97_H0.7160.58
21_I175_V0.7020.57
14_P91_A0.7000.56
18_G185_F0.7000.56
38_N42_S0.6990.56
48_Q66_H0.6960.56
37_I139_L0.6960.56
150_L203_C0.6960.56
72_H75_G0.6900.55
123_A198_P0.6890.55
166_K169_L0.6850.55
185_F190_Q0.6830.55
22_L177_V0.6720.53
35_R42_S0.6650.53
100_C137_P0.6640.52
44_S47_R0.6630.52
68_G89_W0.6590.52
63_N128_L0.6590.52
32_Q35_R0.6580.52
22_L25_F0.6580.52
67_L211_Q0.6550.52
24_R27_F0.6540.51
43_Q87_K0.6500.51
87_K102_R0.6470.51
40_V205_Y0.6450.50
35_R113_Q0.6440.50
106_A168_L0.6440.50
55_Y156_F0.6420.50
132_Q184_L0.6420.50
41_A205_Y0.6400.50
67_L80_P0.6370.50
30_A203_C0.6360.49
104_A109_Y0.6350.49
121_R201_I0.6350.49
31_E43_Q0.6350.49
37_I41_A0.6350.49
28_N160_K0.6320.49
100_C104_A0.6260.48
13_E21_I0.6250.48
26_A99_L0.6230.48
97_H106_A0.6120.47
196_F202_D0.6080.46
61_M72_H0.6030.46
65_G208_T0.6020.46
128_L153_I0.5960.45
159_L162_N0.5930.45
80_P116_A0.5930.45
68_G72_H0.5920.45
49_M61_M0.5920.45
30_A196_F0.5900.44
17_A139_L0.5890.44
46_F128_L0.5800.43
78_Y82_D0.5790.43
66_H97_H0.5790.43
73_R83_P0.5780.43
54_G65_G0.5760.43
67_L81_I0.5760.43
50_V94_Q0.5740.43
29_A119_I0.5720.42
62_T138_D0.5700.42
99_L175_V0.5700.42
100_C112_F0.5700.42
15_L160_K0.5680.42
50_V73_R0.5660.42
56_T202_D0.5660.42
73_R77_L0.5650.42
123_A203_C0.5650.42
38_N94_Q0.5640.42
63_N67_L0.5640.42
64_C119_I0.5630.42
39_D160_K0.5630.42
76_Y79_S0.5630.42
30_A150_L0.5620.41
159_L164_P0.5620.41
34_I200_T0.5620.41
38_N74_Q0.5600.41
21_I125_G0.5590.41
108_G112_F0.5580.41
108_G158_G0.5580.41
153_I194_A0.5570.41
62_T167_R0.5560.41
139_L160_K0.5540.41
129_S208_T0.5540.41
73_R168_L0.5530.40
115_D211_Q0.5520.40
73_R171_E0.5510.40
97_H205_Y0.5510.40
68_G77_L0.5500.40
75_G148_L0.5490.40
127_K166_K0.5480.40
139_L184_L0.5470.40
99_L157_G0.5470.40
92_M199_L0.5460.40
49_M83_P0.5450.40
111_D146_V0.5430.39
37_I152_A0.5420.39
199_L212_A0.5420.39
156_F166_K0.5420.39
109_Y182_S0.5410.39
88_P91_A0.5400.39
27_F119_I0.5400.39
112_F158_G0.5390.39
19_A26_A0.5360.39
81_I91_A0.5340.38
128_L145_S0.5310.38
30_A168_L0.5310.38
151_P197_H0.5310.38
66_H70_T0.5300.38
58_S157_G0.5300.38
158_G162_N0.5300.38
167_R190_Q0.5260.38
87_K192_L0.5260.38
46_F92_M0.5250.38
83_P148_L0.5220.37
97_H195_G0.5190.37
98_N101_Q0.5180.37
68_G82_D0.5170.37
17_A110_P0.5140.36
55_Y134_K0.5120.36
20_V23_R0.5090.36
142_P174_D0.5050.36
45_P90_P0.5030.35
62_T68_G0.5030.35
64_C92_M0.5030.35
39_D42_S0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4jhtA 1 0.9306 100 0.448 Contact Map
4nj4A 1 0.9028 100 0.579 Contact Map
3thtA 3 0.8426 100 0.581 Contact Map
4nplA 1 0.8889 100 0.591 Contact Map
2iuwA 2 0.8981 100 0.603 Contact Map
4qkdA 3 0.8472 100 0.605 Contact Map
3s57A 1 0.875 100 0.613 Contact Map
4ie5A 1 0.8889 99.9 0.728 Contact Map
4bqyA 1 0.8981 96.2 0.915 Contact Map
3kt7A 2 0.9028 95.7 0.919 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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