GREMLIN Database
FUMC - Fumarate hydratase class II
UniProt: P05042 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10358
Length: 467 (403)
Sequences: 10916 (5797)
Seq/√Len: 288.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
60_E70_A3.8851.00
69_K133_D3.4721.00
61_D249_E3.3611.00
53_R78_D3.3421.00
397_R400_Q3.2721.00
57_K74_R3.2241.00
165_K212_L2.9031.00
47_A154_L2.8781.00
57_K253_I2.8461.00
285_A340_V2.7721.00
446_S449_E2.7701.00
168_T209_E2.7591.00
161_I216_E2.6651.00
399_N403_N2.6461.00
170_T389_V2.6421.00
156_L277_A2.6361.00
43_S374_R2.6051.00
113_R117_L2.5281.00
197_L393_P2.5121.00
343_N372_S2.5031.00
221_H276_Q2.4921.00
161_I212_L2.4261.00
249_E252_V2.3951.00
174_K177_A2.3361.00
336_L379_G2.3211.00
282_K344_D2.2901.00
66_S69_K2.2591.00
60_E67_E2.2511.00
153_L223_A2.2471.00
158_K219_L2.1721.00
180_D395_R2.1671.00
223_A257_P2.1671.00
165_K216_E2.0961.00
400_Q404_E2.0921.00
163_Q381_E2.0521.00
339_Q368_N2.0471.00
310_S392_E2.0441.00
168_T212_L2.0241.00
433_L441_A2.0121.00
67_E71_S2.0071.00
170_T384_N1.9971.00
166_T385_K1.9921.00
173_E389_V1.9731.00
79_E117_L1.9711.00
397_R401_L1.9321.00
292_A333_L1.9151.00
212_L216_E1.9091.00
170_T388_A1.9031.00
249_E253_I1.9021.00
286_A341_M1.8971.00
173_E177_A1.8961.00
217_Y221_H1.8701.00
50_L154_L1.8521.00
214_H276_Q1.8261.00
71_S75_Q1.8251.00
427_K431_E1.8031.00
164_L284_L1.7871.00
225_L251_A1.7791.00
217_Y220_P1.7621.00
399_N457_E1.7481.00
172_N209_E1.7391.00
427_K442_L1.7391.00
449_E452_S1.7321.00
53_R74_R1.7081.00
261_A267_A1.7051.00
288_L333_L1.6941.00
178_F181_I1.6931.00
455_R458_Q1.6931.00
420_K423_E1.6751.00
436_K451_D1.6741.00
395_R399_N1.6741.00
224_E259_V1.6681.00
168_T205_V1.6671.00
384_N389_V1.6601.00
70_A74_R1.6571.00
447_E451_D1.6351.00
211_N287_S1.6251.00
275_V351_G1.6221.00
210_H266_E1.6121.00
57_K249_E1.6101.00
248_D252_V1.5901.00
227_L246_V1.5751.00
440_L447_E1.5671.00
166_T384_N1.5391.00
448_A451_D1.5221.00
433_L438_A1.5061.00
58_V249_E1.5061.00
53_R81_L1.4951.00
346_A364_M1.4901.00
163_Q377_A1.4861.00
175_S205_V1.4811.00
72_A118_L1.4791.00
174_K178_F1.4631.00
229_G267_A1.4611.00
220_P257_P1.4611.00
282_K348_N1.4591.00
345_V349_M1.4431.00
218_S280_A1.4221.00
155_A377_A1.4021.00
385_K389_V1.3971.00
330_C387_C1.3761.00
210_H272_D1.3701.00
78_D82_A1.3681.00
278_H347_I1.3641.00
394_N397_R1.3611.00
113_R116_E1.3571.00
271_C362_R1.3471.00
396_E400_Q1.3451.00
213_K221_H1.3371.00
38_E371_Q1.3261.00
46_H50_L1.3251.00
433_L437_A1.3231.00
434_T437_A1.3221.00
292_A383_F1.3181.00
297_W355_N1.3111.00
168_T208_L1.3091.00
53_R57_K1.3011.00
152_A277_A1.3011.00
286_A351_G1.2891.00
420_K444_Y1.2881.00
201_I204_W1.2861.00
43_S155_A1.2841.00
172_N176_R1.2831.00
83_G117_L1.2811.00
161_I219_L1.2801.00
170_T173_E1.2771.00
279_G283_G1.2711.00
197_L201_I1.2651.00
275_V283_G1.2641.00
48_L143_V1.2621.00
166_T389_V1.2531.00
56_A65_L1.2531.00
79_E84_Q1.2461.00
204_W357_E1.2451.00
341_M349_M1.2441.00
181_I395_R1.2431.00
339_Q371_Q1.2271.00
49_A81_L1.2181.00
106_M110_L1.2091.00
137_S238_T1.2081.00
49_A106_M1.2071.00
79_E113_R1.2041.00
415_H449_E1.2011.00
72_A75_Q1.2001.00
176_R179_A1.1981.00
308_E392_E1.1971.00
46_H81_L1.1901.00
64_L136_K1.1871.00
164_L208_L1.1861.00
308_E394_N1.1821.00
342_G346_A1.1791.00
251_A257_P1.1521.00
443_G446_S1.1501.00
347_I369_F1.1501.00
197_L398_I1.1471.00
49_A80_V1.1371.00
178_F395_R1.1351.00
152_A369_F1.1321.00
107_N111_A1.1311.00
333_L379_G1.1311.00
163_Q166_T1.1301.00
344_D348_N1.1261.00
172_N205_V1.1221.00
295_V330_C1.1211.00
163_Q384_N1.1211.00
96_T104_M1.1201.00
191_D302_P1.1161.00
54_A253_I1.1111.00
165_K169_Q1.1101.00
204_W291_I1.1021.00
71_S74_R1.0931.00
215_I284_L1.0921.00
137_S239_H1.0891.00
161_I165_K1.0881.00
46_H85_H1.0851.00
416_I421_A1.0811.00
167_L208_L1.0811.00
179_A198_G1.0771.00
153_L256_A1.0761.00
171_L291_I1.0741.00
72_A76_A1.0701.00
337_C341_M1.0701.00
180_D198_G1.0601.00
274_L369_F1.0571.00
174_K201_I1.0561.00
155_A159_Q1.0551.00
78_D81_L1.0551.00
202_S263_N1.0521.00
166_T381_E1.0511.00
146_T225_L1.0471.00
61_D253_I1.0461.00
51_T143_V1.0441.00
167_L388_A1.0401.00
194_P402_L1.0381.00
183_K398_I1.0341.00
159_Q377_A1.0281.00
74_R78_D1.0251.00
156_L219_L1.0251.00
431_E438_A1.0241.00
67_E70_A1.0211.00
289_M334_T1.0171.00
213_K220_P1.0141.00
47_A150_V1.0141.00
148_M366_I1.0031.00
181_I398_I1.0021.00
223_A256_A0.9981.00
288_L380_M0.9981.00
50_L253_I0.9961.00
148_M274_L0.9941.00
195_L232_V0.9941.00
295_V391_I0.9921.00
46_H154_L0.9921.00
174_K388_A0.9911.00
179_A196_T0.9911.00
191_D301_G0.9881.00
225_L250_L0.9861.00
277_A369_F0.9841.00
417_G420_K0.9841.00
185_G195_L0.9841.00
188_H331_E0.9831.00
433_L442_L0.9761.00
79_E85_H0.9761.00
280_A284_L0.9731.00
415_H453_W0.9711.00
62_L239_H0.9651.00
42_T86_D0.9631.00
310_S394_N0.9611.00
196_T460_V0.9551.00
206_A210_H0.9531.00
153_L219_L0.9521.00
448_A452_S0.9501.00
75_Q79_E0.9501.00
297_W356_F0.9491.00
164_L212_L0.9481.00
219_L223_A0.9461.00
90_P93_I0.9401.00
156_L215_I0.9391.00
213_K217_Y0.9361.00
437_A441_A0.9351.00
79_E83_G0.9351.00
414_T449_E0.9331.00
217_Y276_Q0.9321.00
48_L106_M0.9301.00
75_Q117_L0.9201.00
42_T46_H0.9181.00
60_E71_S0.9171.00
309_I391_I0.9171.00
88_E92_A0.9141.00
427_K438_A0.9141.00
72_A130_P0.9131.00
171_L201_I0.9001.00
46_H86_D0.9001.00
247_A251_A0.8971.00
51_T146_T0.8961.00
186_R191_D0.8921.00
402_L456_P0.8911.00
66_S133_D0.8911.00
58_V246_V0.8901.00
339_Q367_H0.8891.00
43_S154_L0.8881.00
195_L265_F0.8881.00
279_G344_D0.8871.00
50_L81_L0.8851.00
175_S202_S0.8851.00
278_H369_F0.8851.00
58_V62_L0.8831.00
206_A262_P0.8761.00
48_L51_T0.8761.00
438_A442_L0.8711.00
278_H344_D0.8651.00
168_T172_N0.8631.00
154_L158_K0.8631.00
68_E71_S0.8581.00
170_T174_K0.8571.00
129_H132_D0.8571.00
47_A151_A0.8561.00
199_Q264_K0.8501.00
171_L205_V0.8501.00
200_E357_E0.8481.00
177_A392_E0.8471.00
205_V209_E0.8441.00
77_A80_V0.8441.00
305_G397_R0.8431.00
436_K447_E0.8421.00
289_M352_A0.8381.00
284_L380_M0.8361.00
169_Q173_E0.8341.00
189_L297_W0.8341.00
182_V460_V0.8331.00
405_S410_T0.8331.00
436_K440_L0.8321.00
279_G348_N0.8311.00
50_L250_L0.8311.00
449_E453_W0.8281.00
181_I393_P0.8281.00
439_A442_L0.8211.00
61_D67_E0.8211.00
56_A70_A0.8191.00
171_L388_A0.8091.00
182_V402_L0.8080.99
367_H371_Q0.8000.99
333_L383_F0.7980.99
148_M369_F0.7980.99
224_E273_A0.7960.99
55_A138_Q0.7950.99
230_T320_I0.7930.99
381_E385_K0.7920.99
111_A114_A0.7910.99
309_I390_G0.7890.99
73_I130_P0.7890.99
286_A348_N0.7850.99
291_I383_F0.7810.99
427_K441_A0.7790.99
200_E298_L0.7770.99
115_S131_N0.7760.99
178_F393_P0.7750.99
423_E427_K0.7750.99
338_C346_A0.7730.99
104_M107_N0.7720.99
186_R302_P0.7710.99
270_T287_S0.7680.99
403_N436_K0.7670.99
69_K73_I0.7670.99
76_A79_E0.7660.99
83_G113_R0.7650.99
405_S408_L0.7640.99
189_L300_S0.7630.99
82_A86_D0.7620.99
85_H113_R0.7600.99
158_K256_A0.7590.99
210_H224_E0.7590.99
343_N371_Q0.7590.99
450_F454_V0.7530.99
339_Q343_N0.7510.99
60_E65_L0.7490.99
276_Q279_G0.7490.99
226_A267_A0.7480.99
227_L250_L0.7400.99
183_K200_E0.7390.99
405_S411_A0.7360.99
177_A395_R0.7340.99
341_M345_V0.7290.99
260_T264_K0.7290.99
437_A447_E0.7280.99
164_L380_M0.7280.99
57_K61_D0.7270.99
223_A251_A0.7270.99
350_G364_M0.7260.99
298_L356_F0.7250.99
83_G88_E0.7240.99
434_T438_A0.7220.99
53_R253_I0.7210.99
61_D239_H0.7210.99
203_G290_K0.7200.99
387_C391_I0.7190.99
181_I196_T0.7190.99
81_L85_H0.7180.99
265_F357_E0.7170.99
269_A357_E0.7170.99
310_S390_G0.7150.99
68_E72_A0.7130.99
277_A281_L0.7100.99
346_A368_N0.7100.99
272_D287_S0.7100.99
224_E261_A0.7100.99
93_I115_S0.7090.99
246_V250_L0.7090.99
259_V262_P0.7080.99
343_N373_V0.7070.99
292_A330_C0.7070.99
338_C350_G0.7040.99
312_P390_G0.7020.99
396_E399_N0.7000.99
54_A57_K0.6980.99
44_L144_F0.6970.99
180_D457_E0.6950.98
169_Q172_N0.6940.98
393_P398_I0.6930.98
266_E272_D0.6930.98
184_I304_C0.6920.98
310_S313_E0.6920.98
201_I398_I0.6910.98
438_A441_A0.6910.98
65_L136_K0.6900.98
156_L218_S0.6890.98
277_A374_R0.6880.98
406_L409_V0.6860.98
105_N110_L0.6860.98
207_M287_S0.6850.98
275_V347_I0.6830.98
208_L380_M0.6820.98
220_P223_A0.6800.98
159_Q163_Q0.6800.98
445_L453_W0.6790.98
339_Q375_L0.6790.98
69_K130_P0.6790.98
73_I77_A0.6780.98
116_E119_G0.6740.98
76_A118_L0.6710.98
173_E176_R0.6690.98
171_L208_L0.6680.98
40_M367_H0.6670.98
149_H273_A0.6660.98
411_A415_H0.6660.98
274_L347_I0.6650.98
208_L211_N0.6650.98
423_E442_L0.6650.98
53_R70_A0.6640.98
160_L377_A0.6620.98
380_M384_N0.6620.98
406_L410_T0.6620.98
160_L373_V0.6600.98
305_G394_N0.6590.98
247_A260_T0.6580.98
62_L249_E0.6560.98
214_H272_D0.6560.98
80_V110_L0.6560.98
207_M270_T0.6550.98
153_L257_P0.6530.98
167_L384_N0.6520.98
181_I399_N0.6520.98
38_E367_H0.6510.98
130_P133_D0.6470.98
291_I388_A0.6470.98
103_N312_P0.6440.98
82_A85_H0.6430.98
250_L253_I0.6410.97
293_N334_T0.6410.97
64_L239_H0.6410.97
112_N116_E0.6400.97
301_G305_G0.6400.97
84_Q89_F0.6400.97
165_K209_E0.6390.97
213_K216_E0.6390.97
211_N283_G0.6360.97
457_E461_G0.6350.97
64_L137_S0.6350.97
284_L376_L0.6340.97
183_K195_L0.6300.97
111_A115_S0.6290.97
275_V348_N0.6270.97
416_I420_K0.6250.97
422_A426_K0.6250.97
266_E287_S0.6240.97
333_L387_C0.6240.97
56_A73_I0.6230.97
410_T413_N0.6220.97
175_S179_A0.6220.97
85_H88_E0.6200.97
178_F197_L0.6180.97
341_M348_N0.6180.97
288_L384_N0.6180.97
155_A373_V0.6170.97
369_F373_V0.6170.97
57_K70_A0.6170.97
452_S458_Q0.6160.97
397_R404_E0.6150.97
345_V348_N0.6150.97
165_K168_T0.6140.97
156_L222_V0.6140.97
69_K72_A0.6130.97
56_A134_V0.6120.97
166_T170_T0.6120.97
70_A73_I0.6120.97
282_K286_A0.6100.97
160_L281_L0.6100.97
60_E74_R0.6100.97
401_L404_E0.6100.97
184_I402_L0.6080.97
105_N111_A0.6070.96
176_R180_D0.6070.96
215_I280_A0.6070.96
151_A373_V0.6060.96
170_T385_K0.6060.96
289_M353_S0.6060.96
329_Q383_F0.6050.96
289_M349_M0.6050.96
207_M269_A0.6030.96
95_Q109_V0.6020.96
445_L450_F0.6020.96
246_V249_E0.6010.96
214_H217_Y0.6010.96
281_L376_L0.6010.96
338_C349_M0.6000.96
156_L161_I0.6000.96
65_L69_K0.6000.96
218_S222_V0.5990.96
307_G394_N0.5990.96
403_N434_T0.5990.96
433_L439_A0.5980.96
275_V279_G0.5970.96
218_S277_A0.5970.96
225_L260_T0.5960.96
174_K391_I0.5950.96
191_D405_S0.5940.96
196_T461_G0.5930.96
167_L380_M0.5920.96
342_G345_V0.5890.96
314_N390_G0.5890.96
213_K224_E0.5880.96
440_L451_D0.5880.96
283_G351_G0.5870.96
162_P166_T0.5860.96
412_L425_A0.5860.96
77_A110_L0.5850.96
405_S412_L0.5840.96
93_I96_T0.5840.96
172_N175_S0.5820.96
88_E91_L0.5810.95
129_H419_D0.5800.95
346_A365_V0.5790.95
412_L450_F0.5780.95
346_A350_G0.5780.95
405_S409_V0.5770.95
214_H221_H0.5760.95
407_M410_T0.5760.95
208_L284_L0.5760.95
167_L170_T0.5750.95
133_D136_K0.5740.95
204_W207_M0.5740.95
65_L73_I0.5720.95
395_R398_I0.5650.95
200_E294_D0.5640.95
90_P94_W0.5620.95
66_S70_A0.5620.95
51_T147_A0.5600.95
458_Q461_G0.5600.95
445_L449_E0.5600.95
427_K433_L0.5600.95
329_Q387_C0.5590.94
277_A373_V0.5590.94
108_E112_N0.5590.94
407_M411_A0.5580.94
440_L450_F0.5570.94
208_L288_L0.5570.94
164_L215_I0.5550.94
160_L376_L0.5550.94
211_N214_H0.5550.94
285_A376_L0.5550.94
334_T361_F0.5550.94
402_L407_M0.5530.94
209_E213_K0.5520.94
368_N371_Q0.5510.94
305_G309_I0.5510.94
411_A414_T0.5500.94
161_I280_A0.5500.94
279_G282_K0.5490.94
314_N385_K0.5490.94
366_I370_L0.5490.94
101_Q105_N0.5480.94
45_I84_Q0.5460.94
107_N110_L0.5450.94
437_A440_L0.5450.94
281_L284_L0.5450.94
80_V86_D0.5440.94
292_A334_T0.5440.94
452_S455_R0.5440.94
220_P224_E0.5420.94
206_A209_E0.5410.93
91_L94_W0.5380.93
424_I442_L0.5370.93
47_A50_L0.5360.93
183_K298_L0.5360.93
276_Q283_G0.5350.93
49_A77_A0.5340.93
152_A222_V0.5320.93
60_E66_S0.5320.93
231_A320_I0.5310.93
43_S159_Q0.5280.93
50_L54_A0.5280.93
336_L375_L0.5270.93
118_L130_P0.5260.93
89_F109_V0.5260.93
201_I391_I0.5250.92
424_I439_A0.5240.92
214_H283_G0.5230.92
51_T227_L0.5230.92
195_L199_Q0.5210.92
182_V456_P0.5210.92
419_D423_E0.5200.92
89_F94_W0.5190.92
431_E441_A0.5190.92
218_S276_Q0.5170.92
89_F92_A0.5170.92
226_A268_L0.5160.92
181_I198_G0.5160.92
50_L254_T0.5130.92
278_H372_S0.5120.91
251_A256_A0.5120.91
297_W300_S0.5110.91
400_Q403_N0.5110.91
56_A74_R0.5090.91
281_L340_V0.5070.91
103_N107_N0.5060.91
92_A95_Q0.5060.91
140_S226_A0.5060.91
216_E220_P0.5050.91
333_L337_C0.5040.91
105_N109_V0.5030.91
188_H360_V0.5030.91
73_I118_L0.5020.91
340_V377_A0.5010.91
55_A250_L0.5010.91
189_L355_N0.5000.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1furA 4 0.9807 100 0.133 Contact Map
1yfmA 4 0.9572 100 0.142 Contact Map
1vdkA 4 0.9786 100 0.144 Contact Map
3r6qA 4 0.9893 100 0.15 Contact Map
4hgvA 4 0.985 100 0.151 Contact Map
3gtdA 4 0.9743 100 0.153 Contact Map
1jswA 4 0.9764 100 0.156 Contact Map
3e04A 4 0.985 100 0.159 Contact Map
3ocfA 4 0.9572 100 0.171 Contact Map
4admA 4 0.97 100 0.174 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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