GREMLIN Database
GSHB - Glutathione synthetase
UniProt: P04425 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10419
Length: 316 (269)
Sequences: 10199 (5254)
Seq/√Len: 320.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
137_E199_N3.9681.00
152_E177_D3.8761.00
148_K181_G3.5971.00
103_E120_R2.8041.00
103_E107_E2.7331.00
176_G179_N2.6821.00
143_N146_Q2.6401.00
248_T251_D2.6251.00
249_E253_K2.6201.00
253_K257_Q2.4601.00
73_Q76_P2.4601.00
257_Q261_T2.3691.00
174_K202_P2.0621.00
72_E75_L2.0441.00
139_L153_K2.0171.00
208_D212_L2.0141.00
235_N238_A2.0001.00
224_A227_P1.9861.00
149_A153_K1.9841.00
234_G237_A1.9471.00
30_R303_G1.9201.00
241_R244_P1.9111.00
210_R214_V1.9101.00
120_R127_F1.8811.00
286_S301_I1.8681.00
252_W256_R1.8331.00
148_K177_D1.8111.00
139_L150_F1.7891.00
106_E116_P1.7871.00
177_D181_G1.7871.00
210_R213_V1.7861.00
240_G244_P1.7721.00
240_G243_E1.7661.00
256_R260_P1.7501.00
237_A240_G1.7371.00
134_L138_T1.7121.00
267_L270_V1.7051.00
222_C225_R1.6921.00
254_I257_Q1.6901.00
181_G185_E1.6861.00
242_G245_R1.6821.00
31_R307_D1.6781.00
104_R108_K1.6761.00
241_R245_R1.6611.00
105_A110_T1.6141.00
150_F154_H1.6031.00
106_E115_K1.6001.00
115_K268_I1.5911.00
225_R228_Q1.5831.00
222_C227_P1.5741.00
188_T193_R1.5701.00
43_L46_I1.5461.00
146_Q149_A1.5171.00
173_V183_I1.5071.00
208_D213_V1.4991.00
208_D214_V1.4911.00
122_C282_I1.4891.00
174_K179_N1.4631.00
134_L137_E1.4521.00
268_I271_G1.4391.00
72_E76_P1.4311.00
27_E303_G1.4201.00
132_S258_I1.4191.00
223_L227_P1.4161.00
234_G238_A1.4151.00
230_G233_R1.4131.00
222_C226_I1.4131.00
248_T252_W1.4121.00
73_Q77_L1.4121.00
235_N239_G1.4051.00
117_Q121_D1.4021.00
223_L226_I1.3921.00
260_P265_K1.3881.00
184_A195_C1.3611.00
238_A241_R1.3411.00
172_R200_Y1.3391.00
127_F130_W1.3301.00
234_G239_G1.3231.00
216_G220_P1.3061.00
147_L184_A1.3001.00
258_I262_L1.2921.00
235_N240_G1.2841.00
115_K118_S1.2791.00
266_G271_G1.2721.00
182_V186_T1.2641.00
148_K152_E1.2641.00
224_A228_Q1.2611.00
27_E30_R1.2541.00
236_L240_G1.2501.00
157_I197_A1.2461.00
266_G269_F1.2451.00
151_W157_I1.2451.00
121_D131_F1.2351.00
239_G244_P1.2341.00
257_Q260_P1.2341.00
150_F153_K1.2281.00
144_K181_G1.2271.00
217_E220_P1.1981.00
226_I229_G1.1971.00
71_G75_L1.1841.00
71_G74_D1.1831.00
28_A35_L1.1791.00
144_K148_K1.1791.00
137_E153_K1.1741.00
216_G219_V1.1631.00
126_L130_W1.1591.00
236_L239_G1.1541.00
225_R229_G1.1441.00
132_S279_L1.1431.00
266_G270_V1.1391.00
267_L271_G1.1351.00
86_R89_P1.1311.00
237_A241_R1.1171.00
229_G232_T1.1161.00
238_A243_E1.1101.00
250_S254_I1.1091.00
233_R238_A1.1021.00
134_L278_R1.0901.00
101_I120_R1.0881.00
219_V265_K1.0871.00
134_L258_I1.0861.00
240_G245_R1.0861.00
211_V259_G1.0831.00
287_P290_I1.0811.00
249_E252_W1.0761.00
235_N241_R1.0741.00
234_G240_G1.0681.00
232_T235_N1.0661.00
211_V274_I1.0651.00
173_V176_G1.0611.00
35_L38_M1.0591.00
34_E54_T1.0571.00
254_I258_I1.0531.00
253_K256_R1.0511.00
144_K185_E1.0471.00
89_P92_D1.0461.00
147_L197_A1.0411.00
118_S268_I1.0381.00
233_R236_L1.0261.00
162_L194_Y1.0251.00
145_A148_K1.0231.00
237_A244_P1.0151.00
104_R107_E1.0091.00
142_R146_Q1.0061.00
239_G243_E1.0051.00
304_M307_D0.9991.00
227_P230_G0.9961.00
61_Q64_E0.9891.00
250_S253_K0.9841.00
151_W180_L0.9831.00
141_T147_L0.9791.00
286_S289_C0.9791.00
233_R237_A0.9791.00
247_L251_D0.9631.00
117_Q127_F0.9601.00
303_G307_D0.9581.00
232_T237_A0.9571.00
211_V227_P0.9561.00
86_R91_F0.9551.00
74_D78_A0.9541.00
156_D172_R0.9511.00
130_W133_D0.9451.00
236_L243_E0.9431.00
213_V216_G0.9411.00
286_S290_I0.9401.00
85_M89_P0.9381.00
86_R90_P0.9311.00
159_L180_L0.9301.00
215_D220_P0.9291.00
129_A135_T0.9281.00
151_W177_D0.9241.00
149_A152_E0.9231.00
228_Q232_T0.9221.00
184_A188_T0.9211.00
85_M90_P0.9161.00
86_R92_D0.9151.00
145_A149_A0.9141.00
103_E116_P0.9141.00
87_K91_F0.9051.00
39_E42_D0.9021.00
87_K92_D0.8971.00
187_L191_G0.8921.00
255_A258_I0.8891.00
239_G242_G0.8871.00
85_M92_D0.8841.00
210_R216_G0.8841.00
227_P232_T0.8781.00
236_L241_R0.8751.00
237_A243_E0.8741.00
163_D192_T0.8721.00
231_E235_N0.8711.00
255_A260_P0.8651.00
199_N278_R0.8651.00
129_A198_Q0.8651.00
247_L250_S0.8641.00
72_E77_L0.8641.00
223_L228_Q0.8591.00
238_A244_P0.8571.00
168_A190_H0.8541.00
186_T189_E0.8541.00
247_L252_W0.8541.00
224_A229_G0.8521.00
236_L242_G0.8521.00
121_D127_F0.8511.00
134_L254_I0.8421.00
250_S278_R0.8401.00
84_L87_K0.8401.00
71_G76_P0.8391.00
147_L180_L0.8381.00
232_T236_L0.8371.00
87_K90_P0.8351.00
106_E117_Q0.8321.00
239_G245_R0.8271.00
103_E106_E0.8261.00
142_R194_Y0.8241.00
84_L89_P0.8151.00
99_T120_R0.8101.00
39_E43_L0.8101.00
34_E53_H0.8061.00
226_I230_G0.8041.00
79_D108_K0.8031.00
288_T292_E0.8021.00
42_D46_I0.7981.00
144_K184_A0.7971.00
215_D219_V0.7971.00
75_L110_T0.7961.00
83_I90_P0.7951.00
231_E236_L0.7951.00
126_L162_L0.7881.00
289_C292_E0.7871.00
254_I278_R0.7861.00
212_L216_G0.7781.00
230_G234_G0.7781.00
53_H56_T0.7741.00
222_C228_Q0.7701.00
85_M98_A0.7650.99
118_S121_D0.7620.99
83_I89_P0.7590.99
85_M91_F0.7550.99
238_A242_G0.7540.99
48_G52_A0.7510.99
288_T291_R0.7490.99
261_T265_K0.7490.99
285_T288_T0.7480.99
208_D216_G0.7480.99
225_R230_G0.7450.99
62_N72_E0.7420.99
105_A112_I0.7420.99
158_I170_I0.7410.99
251_D277_D0.7400.99
234_G241_R0.7350.99
84_L90_P0.7350.99
40_M56_T0.7280.99
185_E189_E0.7270.99
51_R54_T0.7230.99
230_G235_N0.7230.99
252_W262_L0.7230.99
255_A261_T0.7220.99
76_P108_K0.7170.99
75_L80_L0.7150.99
88_D91_F0.7150.99
236_L244_P0.7150.99
267_L282_I0.7140.99
83_I86_R0.7120.99
55_R78_A0.7110.99
251_D254_I0.7080.99
169_S187_L0.7070.99
88_D92_D0.7050.99
126_L140_V0.7030.99
285_T289_C0.7020.99
173_V180_L0.6970.99
188_T195_C0.6960.99
259_G262_L0.6950.99
80_L83_I0.6940.99
71_G77_L0.6940.99
59_V64_E0.6920.99
275_I280_T0.6910.99
299_V304_M0.6870.99
37_Y40_M0.6860.99
22_F25_L0.6830.99
220_P223_L0.6820.99
84_L92_D0.6800.99
103_E117_Q0.6790.99
159_L197_A0.6780.99
75_L79_D0.6780.99
171_F190_H0.6780.99
127_F131_F0.6770.99
287_P291_R0.6770.99
249_E256_R0.6730.99
180_L197_A0.6710.99
217_E304_M0.6710.99
38_M42_D0.6710.99
36_H80_L0.6690.99
129_A196_M0.6670.99
118_S266_G0.6640.99
43_L48_G0.6630.99
205_K215_D0.6580.98
294_E302_T0.6570.98
145_A181_G0.6540.98
24_M84_L0.6510.98
56_T60_K0.6500.98
83_I105_A0.6500.98
294_E301_I0.6500.98
300_S303_G0.6480.98
102_L116_P0.6450.98
159_L183_I0.6450.98
231_E237_A0.6440.98
287_P292_E0.6410.98
81_D92_D0.6340.98
129_A138_T0.6330.98
270_V273_D0.6310.98
42_D45_L0.6280.98
272_L282_I0.6250.98
27_E31_R0.6250.98
30_R307_D0.6240.98
211_V222_C0.6240.98
255_A259_G0.6220.98
231_E234_G0.6190.98
52_A55_R0.6170.98
84_L91_F0.6170.98
272_L275_I0.6130.98
187_L190_H0.6110.98
33_Y306_M0.6090.97
55_R74_D0.6090.97
237_A242_G0.6080.97
74_D80_L0.6050.97
72_E104_R0.6030.97
265_K269_F0.6020.97
132_S135_T0.6010.97
225_R232_T0.6010.97
158_I172_R0.6010.97
251_D255_A0.5990.97
48_G54_T0.5980.97
171_F186_T0.5970.97
224_A230_G0.5970.97
83_I92_D0.5970.97
38_M74_D0.5960.97
45_L48_G0.5950.97
258_I261_T0.5940.97
215_D218_P0.5910.97
255_A262_L0.5900.97
124_E194_Y0.5880.97
131_F266_G0.5850.97
208_D215_D0.5850.97
232_T239_G0.5850.97
83_I87_K0.5850.97
129_A133_D0.5810.97
228_Q231_E0.5800.97
117_Q120_R0.5800.97
141_T150_F0.5790.97
235_N242_G0.5780.97
83_I110_T0.5760.96
187_L195_C0.5740.96
121_D266_G0.5710.96
81_D84_L0.5700.96
285_T290_I0.5690.96
133_D137_E0.5690.96
227_P231_E0.5680.96
25_L35_L0.5680.96
211_V273_D0.5670.96
25_L29_Q0.5670.96
85_M88_D0.5640.96
141_T197_A0.5640.96
47_N51_R0.5580.96
122_C267_L0.5540.96
71_G78_A0.5530.96
83_I91_F0.5510.96
119_L284_V0.5470.95
214_V219_V0.5470.95
223_L229_G0.5460.95
131_F262_L0.5440.95
58_N61_Q0.5440.95
163_D194_Y0.5430.95
55_R59_V0.5430.95
209_K251_D0.5420.95
50_A53_H0.5420.95
49_E52_A0.5400.95
212_L301_I0.5360.95
62_N104_R0.5350.95
81_D85_M0.5350.95
116_P120_R0.5340.95
269_F305_L0.5340.95
219_V225_R0.5330.95
51_R55_R0.5320.95
82_V85_M0.5320.95
183_I186_T0.5320.95
157_I173_V0.5290.94
150_F197_A0.5270.94
55_R58_N0.5240.94
36_H54_T0.5230.94
102_L119_L0.5230.94
235_N243_E0.5210.94
36_H39_E0.5190.94
231_E238_A0.5180.94
293_I301_I0.5160.94
56_T59_V0.5160.94
246_P250_S0.5110.93
229_G234_G0.5110.93
141_T146_Q0.5070.93
100_Y104_R0.5020.93
252_W255_A0.5020.93
285_T292_E0.5010.93
100_Y116_P0.5000.93
74_D77_L0.5000.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1gsaA 3 0.9937 100 0.268 Contact Map
4iwxA 3 0.8956 100 0.42 Contact Map
3vpbA 3 0.8513 100 0.435 Contact Map
3lwbA 2 0.8829 100 0.47 Contact Map
3e5nA 2 0.9051 100 0.481 Contact Map
3vpdA 3 0.8513 100 0.481 Contact Map
3i12A 2 0.9494 100 0.481 Contact Map
1e4eA 4 0.943 100 0.482 Contact Map
3k3pA 2 0.8861 100 0.489 Contact Map
3r5xA 2 0.8734 100 0.489 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0096 seconds.