GREMLIN Database
PHOE - Outer membrane pore protein E
UniProt: P02932 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10729
Length: 351 (321)
Sequences: 5502 (3979)
Seq/√Len: 222.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
35_Y60_G3.6991.00
63_G75_G3.4651.00
286_Y304_N3.3361.00
72_T103_K3.2951.00
198_G201_D3.2501.00
76_R99_F3.1421.00
64_E72_T3.1291.00
74_Y101_G3.0901.00
280_L310_A3.0001.00
242_I274_Y2.7601.00
156_N168_L2.7441.00
324_Y341_D2.6631.00
206_G231_W2.4621.00
248_Y266_Q2.4541.00
121_D153_T2.3971.00
38_V83_G2.3641.00
154_Y157_T2.3301.00
210_N229_E2.2891.00
62_K76_R2.2851.00
250_E266_Q2.2711.00
288_L304_N2.2641.00
40_A83_G2.2641.00
118_A171_Q2.2501.00
74_Y109_S2.2461.00
281_R313_Y2.2221.00
74_Y111_D2.1821.00
314_F320_A2.1681.00
170_L191_T2.1451.00
311_T319_S2.1311.00
204_I235_L2.1271.00
168_L193_L2.1161.00
34_V61_F2.0781.00
319_S348_T2.0471.00
280_L312_Y2.0381.00
276_F282_P2.0381.00
151_L171_Q1.9521.00
118_A169_T1.9251.00
114_R149_S1.9251.00
103_K109_S1.9211.00
35_Y78_E1.8921.00
71_L102_L1.8921.00
97_L116_L1.8901.00
16_S20_Q1.8901.00
239_A244_L1.8501.00
71_L106_D1.8441.00
90_T95_T1.8371.00
156_N163_I1.8331.00
167_N194_T1.8251.00
206_G233_T1.8121.00
162_V167_N1.7851.00
35_Y76_R1.7131.00
37_K58_R1.6991.00
84_N176_N1.6931.00
112_Y152_A1.6661.00
320_A347_M1.6561.00
79_A93_Q1.6321.00
39_K321_F1.6281.00
318_M349_Y1.6231.00
110_F152_A1.6171.00
328_Q339_N1.6091.00
208_Y231_W1.6011.00
157_T163_I1.5881.00
92_Q149_S1.5791.00
35_Y62_K1.5751.00
80_E96_R1.5681.00
210_N227_R1.5571.00
74_Y103_K1.5531.00
67_I73_G1.5511.00
178_N181_V1.5501.00
241_N274_Y1.4971.00
235_L246_T1.4931.00
319_S350_Q1.4851.00
273_Q281_R1.4741.00
282_P310_A1.4701.00
155_R169_T1.4611.00
202_F237_Y1.4531.00
58_R96_R1.4421.00
244_L272_A1.4321.00
317_N349_Y1.4131.00
66_Q70_Q1.4121.00
245_A271_V1.4041.00
58_R78_E1.4001.00
108_G154_Y1.3941.00
72_T105_K1.3761.00
122_V205_S1.3741.00
111_D155_R1.3711.00
250_E264_K1.3611.00
326_I339_N1.3611.00
236_K245_A1.3461.00
111_D153_T1.3401.00
107_L154_Y1.3351.00
59_F79_A1.3081.00
78_E96_R1.3041.00
148_A188_G1.3031.00
197_F201_D1.2981.00
115_N151_L1.2961.00
34_V59_F1.2961.00
247_F269_E1.2901.00
156_N165_G1.2801.00
74_Y99_F1.2801.00
126_T203_A1.2771.00
111_D115_N1.2631.00
326_I341_D1.2581.00
288_L302_L1.2541.00
204_I233_T1.2451.00
201_D237_Y1.2431.00
242_I246_T1.2401.00
272_A284_L1.2391.00
65_T315_N1.2301.00
97_L115_N1.2281.00
290_K302_L1.2241.00
76_R97_L1.2141.00
202_F235_L1.2081.00
172_Y189_F1.2071.00
55_S348_T1.2051.00
152_A174_G1.2001.00
79_A88_S1.1991.00
173_Q188_G1.1971.00
71_L107_L1.1911.00
277_D280_L1.1881.00
236_K243_Y1.1841.00
286_Y306_I1.1841.00
329_L337_I1.1841.00
276_F280_L1.1791.00
49_S84_N1.1761.00
82_A87_E1.1711.00
151_L173_Q1.1601.00
92_Q95_T1.1591.00
107_L110_F1.1591.00
66_Q72_T1.1531.00
231_W250_E1.1481.00
238_D241_N1.1381.00
171_Q190_G1.1361.00
246_T268_F1.1351.00
196_D203_A1.1351.00
275_Q281_R1.1331.00
279_G313_Y1.1321.00
73_G100_A1.1301.00
321_F346_G1.1301.00
208_Y229_E1.1291.00
41_M53_D1.1261.00
242_I272_A1.1251.00
285_G307_D1.1191.00
10_V13_I1.1191.00
177_E181_V1.1171.00
119_L207_A1.1131.00
37_K350_Q1.1071.00
43_Y344_A1.1051.00
238_D243_Y1.0841.00
38_V81_F1.0771.00
284_L306_I1.0751.00
73_G102_L1.0711.00
99_F111_D1.0681.00
64_E74_Y1.0661.00
32_L75_G1.0641.00
203_A236_K1.0631.00
283_S309_G1.0591.00
40_A49_S1.0541.00
4_S8_L1.0521.00
96_R147_R1.0511.00
232_A249_S1.0501.00
77_W94_K1.0461.00
246_T270_A1.0461.00
303_V329_L1.0421.00
71_L105_K1.0401.00
64_E103_K1.0381.00
109_S153_T1.0361.00
303_V327_N1.0341.00
17_A20_Q1.0331.00
283_S311_T1.0261.00
46_D341_D1.0251.00
322_V345_V1.0181.00
153_T171_Q1.0171.00
302_L330_D1.0171.00
309_G323_D1.0141.00
267_N289_S1.0131.00
41_M344_A0.9981.00
148_A151_L0.9971.00
322_V343_V0.9911.00
65_T317_N0.9831.00
166_L197_F0.9801.00
271_V285_G0.9771.00
166_L193_L0.9751.00
157_T162_V0.9741.00
167_N196_D0.9701.00
31_K64_E0.9701.00
77_W98_A0.9631.00
58_R147_R0.9591.00
238_D274_Y0.9561.00
170_L193_L0.9531.00
170_L189_F0.9521.00
306_I326_I0.9471.00
6_L10_V0.9391.00
313_Y319_S0.9341.00
169_T192_S0.9311.00
117_G148_A0.9291.00
129_F309_G0.9261.00
70_Q105_K0.9210.99
3_K6_L0.9170.99
129_F283_S0.9160.99
37_K348_T0.9150.99
318_M347_M0.9100.99
103_K106_D0.9100.99
115_N153_T0.9080.99
119_L232_A0.9080.99
57_I81_F0.9080.99
40_A84_N0.9060.99
238_D275_Q0.9050.99
284_L308_V0.9040.99
119_L230_A0.9020.99
266_Q292_K0.8950.99
60_G78_E0.8940.99
173_Q176_N0.8930.99
118_A207_A0.8910.99
314_F318_M0.8830.99
192_S207_A0.8800.99
324_Y343_V0.8780.99
241_N275_Q0.8740.99
122_V192_S0.8730.99
7_A10_V0.8700.99
54_Q319_S0.8670.99
276_F310_A0.8590.99
243_Y275_Q0.8480.99
5_T8_L0.8450.99
228_A251_T0.8410.99
30_N65_T0.8390.99
129_F285_G0.8370.99
128_M285_G0.8340.99
55_S350_Q0.8330.99
265_T289_S0.8330.99
88_S93_Q0.8310.99
55_S319_S0.8300.99
33_D62_K0.8290.99
243_Y273_Q0.8290.99
4_S7_A0.8270.99
205_S234_G0.8220.99
153_T169_T0.8210.99
252_R264_K0.8160.99
118_A192_S0.8060.99
279_G310_A0.8050.99
15_A18_S0.8000.99
269_E287_V0.7990.99
155_R167_N0.7990.99
122_V194_T0.7900.98
68_N315_N0.7900.98
307_D325_K0.7850.98
65_T318_M0.7810.98
125_W194_T0.7780.98
128_M269_E0.7770.98
82_A89_D0.7760.98
9_V13_I0.7740.98
245_A273_Q0.7730.98
329_L333_N0.7700.98
41_M55_S0.7690.98
86_A94_K0.7670.98
11_M16_S0.7670.98
121_D155_R0.7660.98
122_V155_R0.7650.98
311_T348_T0.7650.98
118_A190_G0.7620.98
268_F290_K0.7610.98
114_R147_R0.7560.98
248_Y268_F0.7520.98
126_T234_G0.7510.98
123_E232_A0.7500.98
99_F115_N0.7490.98
8_L11_M0.7490.98
251_T265_T0.7480.98
65_T68_N0.7480.98
156_N170_L0.7460.98
267_N287_V0.7450.98
82_A85_K0.7410.98
16_S19_V0.7360.98
39_K52_G0.7350.97
148_A171_Q0.7310.97
239_A242_I0.7290.97
157_T165_G0.7260.97
80_E95_T0.7260.97
109_S155_R0.7220.97
333_N337_I0.7170.97
162_V196_D0.7100.97
302_L328_Q0.7100.97
66_Q69_D0.7080.97
281_R311_T0.7060.97
8_L12_G0.7050.97
266_Q290_K0.7050.97
59_F93_Q0.7040.97
168_L191_T0.7030.97
58_R80_E0.7030.97
128_M271_V0.7030.97
150_G176_N0.7010.97
132_F135_D0.7000.97
190_G209_T0.6980.97
156_N164_D0.6930.96
128_M273_Q0.6910.96
116_L120_Y0.6900.96
70_Q106_D0.6890.96
119_L190_G0.6890.96
276_F279_G0.6860.96
9_V12_G0.6830.96
328_Q338_N0.6830.96
345_V349_Y0.6810.96
264_K290_K0.6770.96
80_E94_K0.6760.96
308_V324_Y0.6750.96
231_W266_Q0.6720.96
188_G211_S0.6700.96
323_D344_A0.6680.96
117_G190_G0.6680.96
65_T73_G0.6670.96
201_D239_A0.6640.95
60_G96_R0.6640.95
29_G33_D0.6630.95
116_L147_R0.6610.95
273_Q283_S0.6600.95
278_F312_Y0.6600.95
7_A11_M0.6600.95
105_K110_F0.6570.95
310_A322_V0.6560.95
172_Y187_D0.6550.95
12_G20_Q0.6530.95
88_S92_Q0.6530.95
47_N340_D0.6510.95
5_T17_A0.6490.95
84_N150_G0.6480.95
194_T205_S0.6480.95
201_D238_D0.6460.95
5_T9_V0.6460.95
45_S342_I0.6450.95
198_G237_Y0.6440.95
184_Q212_D0.6430.95
43_Y342_I0.6410.95
157_T167_N0.6410.95
30_N66_Q0.6400.94
129_F135_D0.6380.94
65_T349_Y0.6370.94
43_Y48_A0.6350.94
189_F193_L0.6340.94
134_G138_A0.6250.94
59_F81_F0.6240.94
228_A253_K0.6180.93
79_A94_K0.6170.93
233_T248_Y0.6140.93
165_G195_Y0.6140.93
264_K292_K0.6110.93
35_Y96_R0.6110.93
315_N318_M0.6110.93
105_K154_Y0.6080.93
291_G301_D0.6060.93
308_V322_V0.6040.93
80_E147_R0.6040.93
212_D227_R0.6030.93
269_E285_G0.6020.92
134_G137_S0.6010.92
187_D212_D0.6000.92
9_V15_A0.5990.92
183_K187_D0.5980.92
325_K342_I0.5930.92
15_A20_Q0.5930.92
127_D132_F0.5920.92
44_M341_D0.5920.92
156_N162_V0.5910.92
67_I70_Q0.5900.92
100_A315_N0.5900.92
253_K265_T0.5880.92
91_A95_T0.5880.92
107_L157_T0.5880.92
85_K94_K0.5810.91
112_Y115_N0.5810.91
305_Y327_N0.5810.91
122_V169_T0.5800.91
289_S303_V0.5790.91
268_F272_A0.5780.91
275_Q313_Y0.5770.91
44_M326_I0.5770.91
311_T350_Q0.5760.91
132_F136_S0.5760.91
148_A173_Q0.5750.91
101_G111_D0.5750.91
43_Y50_K0.5740.91
4_S15_A0.5730.91
117_G209_T0.5720.90
51_D83_G0.5710.90
46_D337_I0.5710.90
121_D169_T0.5700.90
254_M262_A0.5700.90
127_D133_G0.5670.90
126_T245_A0.5650.90
177_E185_N0.5640.90
12_G15_A0.5630.90
299_D331_S0.5630.90
307_D323_D0.5630.90
32_L63_G0.5610.90
306_I328_Q0.5610.90
44_M343_V0.5580.89
74_Y153_T0.5560.89
57_I83_G0.5550.89
56_Y80_E0.5540.89
253_K256_P0.5530.89
53_D344_A0.5520.89
3_K15_A0.5510.89
241_N276_F0.5500.89
66_Q316_K0.5490.89
49_S52_G0.5490.89
40_A44_M0.5490.89
287_V305_Y0.5490.89
6_L13_I0.5480.89
188_G209_T0.5460.88
163_I167_N0.5440.88
58_R97_L0.5440.88
230_A251_T0.5430.88
293_D301_D0.5430.88
164_D197_F0.5430.88
234_G247_F0.5420.88
45_S48_A0.5420.88
29_G32_L0.5420.88
304_N330_D0.5420.88
268_F288_L0.5410.88
43_Y54_Q0.5400.88
42_H52_G0.5390.88
293_D330_D0.5370.88
29_G66_Q0.5360.87
56_Y130_P0.5360.87
2_K5_T0.5350.87
256_P263_N0.5350.87
31_K34_V0.5340.87
244_L270_A0.5330.87
154_Y163_I0.5320.87
93_Q98_A0.5310.87
13_I18_S0.5310.87
139_Q142_N0.5300.87
156_N166_L0.5290.87
247_F271_V0.5280.87
5_T15_A0.5270.87
117_G141_D0.5260.86
127_D130_P0.5250.86
62_K316_K0.5250.86
190_G229_E0.5250.86
32_L65_T0.5240.86
203_A206_G0.5240.86
6_L9_V0.5230.86
211_S228_A0.5230.86
135_D138_A0.5220.86
43_Y325_K0.5210.86
249_S265_T0.5210.86
217_Q223_G0.5200.86
306_I339_N0.5200.86
203_A238_D0.5190.86
231_W248_Y0.5180.86
65_T70_Q0.5170.86
275_Q279_G0.5160.85
327_N340_D0.5150.85
41_M321_F0.5150.85
31_K63_G0.5150.85
101_G109_S0.5150.85
303_V333_N0.5140.85
56_Y131_E0.5130.85
249_S267_N0.5130.85
182_K187_D0.5130.85
305_Y325_K0.5120.85
197_F204_I0.5120.85
169_T194_T0.5120.85
75_G94_K0.5110.85
154_Y168_L0.5110.85
108_G157_T0.5100.85
134_G305_Y0.5100.85
301_D332_D0.5090.85
162_V168_L0.5080.85
249_S269_E0.5070.84
171_Q188_G0.5060.84
178_N182_K0.5060.84
6_L12_G0.5050.84
54_Q311_T0.5040.84
162_V166_L0.5030.84
39_K94_K0.5030.84
253_K262_A0.5030.84
86_A317_N0.5010.84
54_Q309_G0.5010.84
192_S205_S0.5010.84
53_D56_Y0.5000.84
56_Y116_L0.5000.84
40_A43_Y0.5000.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1phoA 3 0.9288 100 0.249 Contact Map
2j1nA 3 0.9288 100 0.265 Contact Map
2zfgA 3 0.9174 100 0.273 Contact Map
3nsgA 3 0.9202 100 0.276 Contact Map
2fgqX 3 0.8547 100 0.305 Contact Map
3vy8X 3 0.8746 100 0.323 Contact Map
3wi5A 3 0.849 100 0.353 Contact Map
2porA 3 0.8006 100 0.39 Contact Map
3prnA 3 0.7721 100 0.409 Contact Map
2o4vA 3 0.9202 99.4 0.776 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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