GREMLIN Database
ASNA - Aspartate--ammonia ligase
UniProt: P00963 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10091
Length: 330 (311)
Sequences: 694 (398)
Seq/√Len: 22.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
174_L207_D4.8491.00
171_Q175_S4.4571.00
174_L206_S4.2571.00
189_I196_V4.1351.00
266_A271_E4.0691.00
190_A237_L4.0131.00
187_R245_D3.4591.00
203_G267_L3.3971.00
176_R192_D3.2941.00
134_K199_V3.1201.00
319_P322_V3.1161.00
144_I300_L3.1041.00
180_L188_A3.0861.00
239_W244_E3.0311.00
162_P195_A2.8170.99
144_I238_V2.8150.99
258_A285_G2.7110.99
165_I236_I2.7070.99
53_K101_P2.6190.99
262_K274_L2.6180.99
13_S17_S2.5810.99
125_D289_Q2.5690.99
238_V249_L2.5240.98
274_L283_L2.4880.98
320_A324_E2.4870.98
166_H189_I2.4290.98
216_P273_R2.3990.98
121_R136_T2.3920.98
8_K307_L2.3420.97
49_S213_V2.3410.97
86_F92_L2.2300.96
204_K267_L2.2290.96
170_S198_L2.2010.96
282_L285_G2.1940.96
263_H267_L2.1880.96
58_K101_P2.1650.96
166_H193_L2.1560.95
171_Q207_D2.1550.95
60_K104_D2.1150.95
187_R246_A2.0940.95
181_D184_G2.0650.94
281_A286_E1.9930.93
188_A192_D1.9760.93
258_A289_Q1.9710.93
203_G222_S1.9440.92
109_L245_D1.9360.92
130_F200_G1.9130.92
196_V237_L1.9120.92
53_K58_K1.8750.91
275_E280_Q1.8370.90
124_G127_E1.7890.89
53_K102_D1.7550.88
130_F199_V1.7520.87
6_I90_E1.7250.87
197_F234_G1.7230.87
59_V67_F1.7120.86
189_I193_L1.7020.86
240_N247_F1.6930.86
138_E164_Q1.6860.85
271_E274_L1.6840.85
200_G222_S1.6670.85
160_F306_Q1.6640.85
187_R244_E1.6590.84
175_S206_S1.6350.83
141_W195_A1.6280.83
171_Q174_L1.6230.83
88_A124_G1.6130.83
160_F241_P1.5950.82
169_H199_V1.5890.82
157_L306_Q1.5580.80
123_M289_Q1.5410.80
22_Q146_A1.5220.79
71_H215_A1.5170.78
125_D258_A1.5120.78
183_K212_D1.5030.78
127_E132_T1.5030.78
170_S182_A1.5020.78
67_F317_V1.5010.78
31_E97_K1.4810.77
162_P165_I1.4740.76
145_K163_D1.4690.76
18_H150_A1.4570.76
257_D260_T1.4530.75
169_H200_G1.4450.75
82_G286_E1.4100.73
190_A196_V1.4100.73
122_V290_T1.4000.73
222_S260_T1.3980.72
203_G263_H1.3970.72
285_G289_Q1.3790.71
270_D273_R1.3780.71
14_F154_E1.3760.71
16_K95_H1.3320.69
280_Q284_R1.3160.68
244_E247_F1.2990.67
141_W145_K1.2860.66
144_I304_L1.2700.65
108_P308_P1.2680.65
20_S30_I1.2540.64
175_S207_D1.2470.63
262_K266_A1.2220.62
166_H196_V1.2120.61
151_V157_L1.2010.60
189_I198_L1.2000.60
130_F134_K1.1930.60
183_K237_L1.1840.59
150_A304_L1.1840.59
148_E162_P1.1740.59
277_E281_A1.1710.58
47_N273_R1.1660.58
165_I195_A1.1640.58
258_A262_K1.1640.58
125_D257_D1.1470.57
171_Q200_G1.1320.56
323_R326_V1.1210.55
39_R42_D1.1160.55
109_L247_F1.1120.54
14_F150_A1.1110.54
222_S263_H1.1050.54
160_F303_L1.1040.54
141_W303_L1.1000.54
18_H304_L1.0920.53
141_W165_I1.0840.53
20_S33_Q1.0750.52
31_E37_L1.0680.51
259_D263_H1.0680.51
129_Q134_K1.0620.51
148_E159_P1.0570.51
157_L160_F1.0560.51
108_P243_L1.0530.50
144_I249_L1.0510.50
187_R239_W1.0450.50
148_E161_L1.0440.50
138_E163_D1.0420.50
204_K210_R1.0390.49
198_L237_L1.0230.48
11_Q155_F1.0190.48
176_R189_I1.0080.47
238_V247_F1.0060.47
168_V176_R0.9950.46
20_S93_Y0.9910.46
81_L288_P0.9900.46
89_G127_E0.9850.46
121_R139_A0.9840.46
58_K64_D0.9710.45
148_E195_A0.9610.44
216_P270_D0.9590.44
59_V112_V0.9560.44
28_G90_E0.9520.43
26_R146_A0.9500.43
262_K271_E0.9390.43
190_A239_W0.9380.43
26_R143_G0.9360.42
67_F112_V0.9330.42
168_V177_Y0.9310.42
269_G284_R0.9270.42
17_S21_R0.9250.42
53_K215_A0.9250.42
26_R142_A0.9170.41
54_A71_H0.9170.41
195_A303_L0.9090.41
132_T289_Q0.9090.41
82_G281_A0.9070.40
79_Q83_Q0.9040.40
198_L246_A0.9010.40
222_S267_L0.8970.40
121_R132_T0.8960.40
189_I237_L0.8950.40
44_T277_E0.8920.39
141_W194_G0.8900.39
141_W162_P0.8870.39
132_T136_T0.8850.39
45_Q57_V0.8780.39
106_L279_H0.8650.38
260_T263_H0.8640.38
210_R268_T0.8540.37
64_D143_G0.8540.37
23_L27_L0.8510.37
216_P265_L0.8490.37
71_H278_W0.8470.37
47_N215_A0.8360.36
265_L276_L0.8350.36
86_F290_T0.8290.35
9_Q53_K0.8280.35
49_S52_E0.8260.35
13_S24_E0.8240.35
184_G187_R0.8240.35
37_L99_L0.8220.35
149_A159_P0.8210.35
60_K71_H0.8160.35
11_Q80_T0.8130.34
161_L238_V0.8120.34
190_A198_L0.8120.34
68_E93_Y0.8050.34
20_S300_L0.8030.34
263_H266_A0.8030.34
101_P109_L0.7980.33
198_L236_I0.7950.33
276_L282_L0.7910.33
92_L138_E0.7890.33
24_E93_Y0.7880.33
39_R67_F0.7800.32
47_N53_K0.7710.32
56_Q66_Q0.7700.32
188_A191_K0.7690.32
262_K283_L0.7680.32
238_V326_V0.7600.31
166_H190_A0.7560.31
125_D285_G0.7490.31
142_A146_A0.7460.30
143_G147_T0.7440.30
148_E160_F0.7420.30
47_N68_E0.7380.30
10_R150_A0.7380.30
189_I194_G0.7380.30
212_D243_L0.7350.30
102_D276_L0.7340.30
39_R62_L0.7330.30
15_V147_T0.7320.30
98_A117_W0.7230.29
152_S160_F0.7220.29
22_Q25_E0.7220.29
303_L306_Q0.7210.29
129_Q270_D0.7210.29
15_V156_G0.7190.29
148_E306_Q0.7170.29
6_I30_I0.7090.28
170_S200_G0.7080.28
20_S31_E0.7070.28
83_Q323_R0.7050.28
103_E106_L0.7040.28
266_A274_L0.7010.28
148_E165_I0.7000.28
41_G65_A0.6990.28
141_W161_L0.6970.28
14_F18_H0.6950.27
152_S159_P0.6950.27
71_H136_T0.6950.27
5_Y82_G0.6950.27
15_V154_E0.6910.27
5_Y319_P0.6910.27
270_D291_I0.6910.27
265_L273_R0.6860.27
240_N245_D0.6860.27
31_E34_A0.6860.27
16_K27_L0.6850.27
113_Y140_I0.6820.27
57_V95_H0.6800.27
33_Q97_K0.6800.27
45_Q49_S0.6800.27
259_D285_G0.6800.27
182_A209_H0.6780.27
37_L57_V0.6760.26
152_S241_P0.6760.26
41_G44_T0.6750.26
171_Q206_S0.6740.26
97_K300_L0.6730.26
11_Q57_V0.6710.26
28_G127_E0.6700.26
273_R276_L0.6690.26
41_G194_G0.6680.26
16_K23_L0.6640.26
257_D263_H0.6630.26
83_Q281_A0.6590.26
13_S93_Y0.6580.26
240_N243_L0.6560.25
261_L282_L0.6550.25
176_R193_L0.6520.25
85_D90_E0.6520.25
144_I277_E0.6500.25
134_K138_E0.6500.25
9_Q240_N0.6490.25
10_R148_E0.6450.25
201_I211_H0.6440.25
38_S57_V0.6430.25
59_V65_A0.6430.25
280_Q286_E0.6430.25
81_L122_V0.6420.25
176_R180_L0.6420.25
45_Q67_F0.6410.25
61_A106_L0.6410.25
87_S168_V0.6410.25
28_G150_A0.6400.25
30_I86_F0.6390.25
125_D130_F0.6390.25
33_Q45_Q0.6360.24
54_A198_L0.6360.24
129_Q132_T0.6350.24
176_R280_Q0.6330.24
145_K195_A0.6330.24
308_P326_V0.6320.24
37_L71_H0.6320.24
62_L319_P0.6300.24
97_K108_P0.6290.24
31_E113_Y0.6280.24
203_G278_W0.6270.24
173_L246_A0.6270.24
6_I86_F0.6250.24
6_I319_P0.6250.24
137_V254_I0.6210.24
166_H240_N0.6210.24
129_Q321_A0.6190.24
11_Q45_Q0.6190.24
130_F269_G0.6180.23
136_T170_S0.6170.23
6_I9_Q0.6160.23
135_S157_L0.6140.23
213_V270_D0.6130.23
5_Y30_I0.6120.23
319_P325_S0.6120.23
165_I238_V0.6110.23
42_D80_T0.6060.23
25_E28_G0.6050.23
7_A30_I0.6030.23
80_T122_V0.6010.23
45_Q97_K0.6000.23
57_V278_W0.6000.23
180_L187_R0.6000.23
31_E38_S0.6000.23
79_Q82_G0.5990.23
22_Q26_R0.5990.23
71_H129_Q0.5970.22
60_K215_A0.5950.22
145_K162_P0.5940.22
57_V99_L0.5940.22
40_V67_F0.5940.22
7_A51_C0.5930.22
23_L147_T0.5930.22
52_E103_E0.5900.22
238_V303_L0.5900.22
56_Q65_A0.5890.22
158_A306_Q0.5890.22
58_K169_H0.5880.22
239_W303_L0.5860.22
41_G47_N0.5860.22
103_E173_L0.5860.22
68_E244_E0.5850.22
236_I247_F0.5840.22
132_T291_I0.5840.22
149_A153_E0.5840.22
168_V189_I0.5800.22
15_V301_T0.5800.22
39_R49_S0.5790.22
155_F317_V0.5790.22
194_G261_L0.5780.22
159_P306_Q0.5760.21
249_L303_L0.5730.21
216_P272_D0.5730.21
259_D267_L0.5730.21
136_T139_A0.5710.21
270_D276_L0.5700.21
86_F90_E0.5700.21
140_I156_G0.5690.21
34_A215_A0.5670.21
150_A153_E0.5670.21
39_R65_A0.5670.21
157_L300_L0.5660.21
153_E166_H0.5650.21
232_L254_I0.5650.21
68_E190_A0.5640.21
155_F303_L0.5610.21
34_A300_L0.5580.21
61_A64_D0.5580.21
12_I97_K0.5540.20
263_H285_G0.5540.20
212_D265_L0.5540.20
249_L300_L0.5520.20
11_Q15_V0.5520.20
7_A83_Q0.5520.20
278_W291_I0.5510.20
170_S236_I0.5510.20
36_I42_D0.5500.20
96_M117_W0.5500.20
13_S20_S0.5490.20
269_G319_P0.5490.20
282_L286_E0.5490.20
151_V236_I0.5480.20
41_G259_D0.5460.20
13_S301_T0.5460.20
61_A239_W0.5440.20
10_R30_I0.5420.20
22_Q315_C0.5420.20
140_I279_H0.5420.20
13_S31_E0.5410.20
59_V62_L0.5410.20
146_A198_L0.5410.20
148_E194_G0.5400.20
145_K303_L0.5390.20
101_P169_H0.5390.20
131_S179_D0.5390.20
88_A291_I0.5380.20
282_L287_M0.5380.20
42_D317_V0.5370.20
292_G296_G0.5370.20
154_E157_L0.5370.20
175_S268_T0.5360.20
187_R302_M0.5360.20
9_Q315_C0.5350.20
154_E321_A0.5350.20
9_Q20_S0.5340.20
79_Q282_L0.5330.20
17_S20_S0.5320.19
31_E57_V0.5320.19
87_S194_G0.5320.19
45_Q301_T0.5310.19
168_V278_W0.5300.19
216_P276_L0.5300.19
6_I10_R0.5290.19
155_F164_Q0.5290.19
132_T240_N0.5290.19
13_S18_H0.5290.19
175_S200_G0.5280.19
182_A192_D0.5280.19
155_F306_Q0.5270.19
130_F192_D0.5270.19
129_Q281_A0.5270.19
47_N59_V0.5260.19
49_S270_D0.5260.19
307_L312_Q0.5260.19
40_V269_G0.5250.19
175_S284_R0.5240.19
172_E206_S0.5240.19
242_V306_Q0.5230.19
145_K148_E0.5230.19
20_S108_P0.5230.19
23_L124_G0.5230.19
152_S306_Q0.5220.19
168_V192_D0.5210.19
31_E95_H0.5210.19
31_E93_Y0.5210.19
17_S304_L0.5200.19
190_A240_N0.5200.19
135_S198_L0.5200.19
5_Y57_V0.5190.19
97_K301_T0.5190.19
86_F238_V0.5190.19
22_Q79_Q0.5180.19
40_V59_V0.5170.19
26_R139_A0.5170.19
25_E135_S0.5170.19
13_S304_L0.5170.19
135_S139_A0.5160.19
151_V154_E0.5150.19
14_F210_R0.5140.19
21_R69_V0.5110.19
40_V47_N0.5110.19
6_I106_L0.5100.19
106_L322_V0.5100.19
58_K102_D0.5090.19
279_H326_V0.5090.19
17_S93_Y0.5090.19
161_L195_A0.5090.19
79_Q239_W0.5080.18
16_K159_P0.5080.18
99_L278_W0.5080.18
6_I87_S0.5060.18
18_H22_Q0.5050.18
18_H144_I0.5050.18
86_F122_V0.5040.18
165_I303_L0.5040.18
6_I148_E0.5040.18
19_F93_Y0.5040.18
126_G207_D0.5030.18
142_A163_D0.5020.18
13_S319_P0.5010.18
268_T272_D0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
12asA 2 0.9909 100 0.002 Contact Map
4lnsA 2 0.9152 100 0.021 Contact Map
3m4pA 2 0.8515 100 0.827 Contact Map
3nemA 2 0.8606 99.9 0.847 Contact Map
1wydA 2 0.8606 99.9 0.851 Contact Map
1n9wA 2 0.7121 99.4 0.909 Contact Map
2xgtA 2 0.8667 99.2 0.92 Contact Map
1e1oA 2 0.8788 98.9 0.928 Contact Map
4j15A 2 0.7121 98.9 0.928 Contact Map
4ex5A 2 0.8818 98.8 0.931 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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