GREMLIN Database
ASPG2 - L-asparaginase 2
UniProt: P00805 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10046
Length: 348 (313)
Sequences: 3473 (2485)
Seq/√Len: 140.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
121_L133_M4.4211.00
25_N69_N4.1051.00
251_K281_T4.0851.00
240_Y264_A3.7421.00
117_T133_M3.5711.00
73_E94_K3.4211.00
170_V182_V3.3131.00
125_V131_V3.2601.00
163_S211_Y3.0871.00
340_Q343_Q2.9491.00
251_K278_T2.9331.00
27_T102_T2.9261.00
337_D340_Q2.9141.00
78_I87_V2.6661.00
29_L75_V2.6561.00
177_L195_S2.4531.00
331_A344_I2.3851.00
71_K102_T2.3661.00
260_G328_L2.3391.00
25_N71_K2.3111.00
106_V158_A2.2841.00
254_V282_A2.2691.00
85_D244_N2.1981.00
264_A321_P2.1871.00
99_C127_C2.1851.00
171_V176_V2.1841.00
177_L182_V2.1721.00
280_A284_K2.1661.00
237_G258_Y2.1541.00
95_I107_I2.1531.00
30_A36_A2.1411.00
99_C105_F2.0901.00
292_S316_S2.0791.00
99_C129_K2.0521.00
185_T194_K2.0211.00
176_V202_G1.9531.00
27_T98_D1.9181.00
114_M170_V1.8851.00
92_A124_T1.8801.00
157_T176_V1.8681.00
291_R313_F1.8661.00
153_N174_D1.8551.00
29_L94_K1.7721.00
29_L73_E1.7691.00
247_D251_K1.7401.00
54_V72_G1.7301.00
265_G291_R1.7291.00
290_V316_S1.7191.00
88_W243_A1.7191.00
84_N87_V1.7131.00
290_V345_F1.7131.00
122_D180_R1.6971.00
134_V172_M1.6511.00
76_V87_V1.6511.00
331_A341_I1.6411.00
278_T281_T1.6391.00
338_P342_Q1.6261.00
153_N171_V1.6201.00
338_P341_I1.6081.00
75_V94_K1.6061.00
185_T192_T1.5641.00
88_W326_V1.5641.00
85_D329_Q1.5541.00
233_L259_D1.5521.00
120_F124_T1.5451.00
290_V328_L1.5361.00
83_M87_V1.5111.00
249_P253_L1.5071.00
109_H117_T1.4931.00
247_D278_T1.4891.00
55_E59_N1.4821.00
251_K255_D1.4711.00
281_T285_T1.4661.00
53_G56_N1.4521.00
95_I131_V1.4511.00
235_K246_S1.4491.00
238_I321_P1.4441.00
98_D101_K1.4421.00
94_K98_D1.4181.00
157_T171_V1.4111.00
288_A341_I1.4111.00
152_Y156_V1.3971.00
30_A54_V1.3921.00
89_L124_T1.3621.00
292_S321_P1.3361.00
187_T293_S1.3341.00
94_K97_T1.3271.00
250_A282_A1.3181.00
88_W120_F1.2941.00
167_G178_D1.2891.00
239_V250_A1.2831.00
166_R216_A1.2811.00
306_V310_K1.2651.00
235_K248_L1.2641.00
186_N297_T1.2621.00
73_E98_D1.2551.00
92_A96_N1.2521.00
280_A311_Y1.2461.00
175_T191_A1.2391.00
235_K259_D1.2371.00
149_F153_N1.1961.00
145_A148_P1.1931.00
101_K129_K1.1901.00
40_D48_T1.1871.00
271_L279_L1.1660.99
250_A278_T1.1640.99
261_I282_A1.1610.99
204_I209_I1.1610.99
240_Y321_P1.1570.99
78_I83_M1.1530.99
93_K97_T1.1520.99
331_A335_T1.1500.99
82_D273_K1.1470.99
288_A314_V1.1430.99
87_V90_T1.1380.99
30_A74_Q1.1330.99
235_K258_Y1.1160.99
157_T163_S1.1130.99
91_L107_I1.1090.99
52_V140_S1.1090.99
263_S279_L1.1070.99
47_Y50_G1.1060.99
181_D213_R1.1010.99
85_D88_W1.0980.99
252_A256_A1.0960.99
106_V155_V1.0860.99
237_G253_L1.0860.99
253_L258_Y1.0790.99
111_T135_G1.0720.99
93_K229_K1.0720.99
254_V287_T1.0700.99
37_G47_Y1.0630.99
283_A289_V1.0600.99
234_P333_T1.0600.99
106_V159_A1.0510.99
250_A275_V1.0470.99
114_M135_G1.0390.99
95_I105_F1.0310.99
25_N103_D1.0280.99
247_D281_T1.0230.99
26_I103_D1.0200.99
236_V332_L1.0190.99
160_D163_S1.0160.99
43_T47_Y1.0120.99
163_S166_R1.0070.99
241_N272_Y1.0030.98
107_I133_M1.0020.98
335_T344_I0.9990.98
203_Y212_Q0.9870.98
135_G172_M0.9850.98
248_L252_A0.9810.98
24_P161_K0.9790.98
183_T321_P0.9780.98
166_R211_Y0.9770.98
169_L201_L0.9710.98
132_V169_L0.9660.98
283_A312_G0.9650.98
279_L313_F0.9620.98
177_L200_P0.9620.98
105_F129_K0.9620.98
336_K340_Q0.9600.98
86_N90_T0.9600.98
166_R214_T0.9570.98
35_I147_G0.9550.98
116_E294_R0.9460.98
29_L107_I0.9360.98
81_Q270_N0.9350.98
138_R190_V0.9310.98
171_V204_I0.9310.98
294_R322_Q0.9250.97
71_K98_D0.9220.97
76_V91_L0.9210.97
115_E322_Q0.9180.97
113_T266_V0.9130.97
325_R329_Q0.9130.97
48_T139_P0.9120.97
112_D267_G0.9070.97
176_V209_I0.9020.97
68_A159_A0.9010.97
250_A279_L0.8980.97
64_L155_V0.8970.97
127_C131_V0.8950.97
27_T73_E0.8920.97
239_V253_L0.8920.97
96_N227_V0.8910.97
69_N103_D0.8900.97
307_D310_K0.8900.97
119_Y123_L0.8880.97
114_M133_M0.8880.97
163_S169_L0.8810.97
245_A275_V0.8800.97
54_V57_L0.8750.96
241_N275_V0.8700.96
48_T51_K0.8690.96
68_A155_V0.8650.96
26_I159_A0.8640.96
89_L93_K0.8630.96
38_G56_N0.8620.96
241_N263_S0.8600.96
122_D179_G0.8600.96
283_A311_Y0.8480.96
49_V53_G0.8460.96
111_T184_K0.8430.96
279_L283_A0.8430.96
28_I151_L0.8410.95
160_D211_Y0.8410.95
186_N189_D0.8380.95
181_D323_K0.8330.95
95_I99_C0.8330.95
115_E294_R0.8320.95
144_S149_F0.8310.95
340_Q344_I0.8290.95
157_T204_I0.8290.95
67_I152_Y0.8280.95
149_F190_V0.8250.95
328_L341_I0.8210.95
37_G50_G0.8060.94
187_T295_V0.8040.94
28_I108_T0.8030.94
173_N191_A0.7980.94
188_T268_N0.7940.94
85_D243_A0.7940.94
39_G43_T0.7900.94
187_T299_A0.7880.94
302_Q305_E0.7870.94
157_T209_I0.7860.94
62_P67_I0.7860.94
63_Q148_P0.7860.94
175_T200_P0.7860.94
212_Q317_G0.7820.93
308_D313_F0.7820.93
271_L275_V0.7810.93
236_V262_V0.7790.93
169_L176_V0.7780.93
102_T129_K0.7700.93
181_D318_T0.7680.93
119_Y326_V0.7660.93
209_I212_Q0.7650.93
38_G51_K0.7640.93
50_G53_G0.7620.92
183_T320_N0.7610.92
283_A286_G0.7580.92
132_V158_A0.7550.92
292_S324_A0.7540.92
122_D178_D0.7520.92
254_V257_G0.7520.92
126_K226_D0.7480.92
31_T109_H0.7480.92
67_I159_A0.7460.92
102_T105_F0.7440.92
104_G130_P0.7390.91
24_P103_D0.7390.91
175_T194_K0.7380.91
202_G211_Y0.7370.91
169_L211_Y0.7370.91
303_D307_D0.7360.91
36_A108_T0.7350.91
277_D280_A0.7350.91
37_G49_V0.7340.91
241_N266_V0.7330.91
255_D281_T0.7320.91
184_K267_G0.7310.91
324_A345_F0.7300.91
112_D270_N0.7290.91
119_Y180_R0.7280.91
123_L327_L0.7250.90
132_V157_T0.7220.90
259_D287_T0.7220.90
320_N323_K0.7200.90
62_P152_Y0.7190.90
167_G201_L0.7170.90
241_N271_L0.7170.90
319_L327_L0.7160.90
245_A272_Y0.7150.90
62_P68_A0.7150.90
40_D43_T0.7140.90
95_I125_V0.7130.90
35_I136_A0.7120.90
28_I155_V0.7110.90
186_N298_G0.7100.90
118_A170_V0.7100.90
266_V272_Y0.7070.89
185_T296_P0.7070.89
146_D184_K0.7060.89
96_N229_K0.7060.89
260_G324_A0.7060.89
126_K228_S0.7040.89
50_G57_L0.7020.89
321_P324_A0.7010.89
248_L256_A0.7000.89
262_V290_V0.6970.89
277_D281_T0.6960.89
98_D105_F0.6940.88
98_D102_T0.6940.88
27_T71_K0.6930.88
123_L330_L0.6910.88
243_A322_Q0.6880.88
335_T340_Q0.6830.88
134_V154_A0.6810.88
339_Q343_Q0.6810.88
74_Q77_N0.6800.87
92_A120_F0.6790.87
79_G82_D0.6780.87
335_T338_P0.6780.87
254_V278_T0.6750.87
281_T284_K0.6740.87
270_N305_E0.6740.87
181_D327_L0.6730.87
96_N126_K0.6720.87
161_K166_R0.6720.87
81_Q266_V0.6720.87
115_E182_V0.6710.87
90_T94_K0.6690.87
212_Q215_P0.6690.87
302_Q308_D0.6680.87
239_V249_P0.6680.87
166_R169_L0.6670.86
26_I155_V0.6670.86
247_D250_A0.6660.86
107_I121_L0.6640.86
205_H268_N0.6640.86
118_A182_V0.6620.86
85_D89_L0.6620.86
82_D304_A0.6620.86
260_G288_A0.6590.86
268_N301_T0.6580.86
282_A287_T0.6580.86
29_L98_D0.6570.86
337_D344_I0.6560.86
133_M179_G0.6560.86
31_T91_L0.6550.85
140_S147_G0.6540.85
278_T282_A0.6540.85
241_N245_A0.6530.85
178_D181_D0.6530.85
178_D201_L0.6520.85
135_G184_K0.6520.85
288_A338_P0.6490.85
237_G246_S0.6480.85
140_S145_A0.6470.85
88_W117_T0.6460.85
112_D302_Q0.6450.85
139_P142_S0.6440.85
99_C125_V0.6380.84
24_P68_A0.6350.84
323_K327_L0.6350.84
245_A250_A0.6340.84
308_D311_Y0.6320.83
67_I155_V0.6310.83
37_G48_T0.6290.83
268_N291_R0.6290.83
247_D274_S0.6280.83
172_M177_L0.6280.83
76_V90_T0.6270.83
88_W121_L0.6270.83
183_T296_P0.6260.83
154_A171_V0.6260.83
254_V261_I0.6250.83
39_G141_T0.6230.83
270_N304_A0.6200.82
259_D336_K0.6200.82
156_V207_G0.6180.82
137_M192_T0.6180.82
116_E325_R0.6170.82
258_Y287_T0.6140.82
330_L334_Q0.6140.82
228_S334_Q0.6130.82
175_T203_Y0.6130.82
123_L323_K0.6120.81
196_V297_T0.6110.81
50_G56_N0.6100.81
239_V245_A0.6100.81
123_L180_R0.6090.81
36_A54_V0.6080.81
314_V342_Q0.6060.81
35_I108_T0.6050.81
191_A194_K0.6020.80
67_I70_V0.5960.80
267_G270_N0.5960.80
29_L95_I0.5960.80
265_G271_L0.5950.80
71_K103_D0.5930.79
234_P259_D0.5910.79
82_D303_D0.5910.79
89_L229_K0.5900.79
316_S324_A0.5900.79
37_G81_Q0.5890.79
163_S167_G0.5880.79
92_A227_V0.5880.79
70_V106_V0.5860.79
219_H223_T0.5850.78
260_G332_L0.5840.78
263_S271_L0.5840.78
273_K277_D0.5830.78
202_G209_I0.5830.78
87_V91_L0.5810.78
119_Y327_L0.5810.78
283_A313_F0.5800.78
300_T315_A0.5790.78
260_G292_S0.5780.78
92_A125_V0.5760.77
27_T95_I0.5730.77
137_M267_G0.5730.77
176_V211_Y0.5720.77
276_F280_A0.5720.77
80_S109_H0.5720.77
253_L257_G0.5700.77
254_V285_T0.5680.76
307_D311_Y0.5680.76
316_S345_F0.5610.76
188_T299_A0.5590.75
90_T97_T0.5580.75
79_G179_G0.5580.75
91_L94_K0.5570.75
31_T34_T0.5550.75
282_A288_A0.5530.74
114_M154_A0.5530.74
214_T226_D0.5520.74
100_D103_D0.5520.74
75_V244_N0.5490.74
223_T226_D0.5480.74
169_L202_G0.5470.74
137_M172_M0.5470.74
93_K227_V0.5470.74
30_A37_G0.5470.74
137_M184_K0.5460.74
249_P252_A0.5460.74
119_Y179_G0.5450.73
61_V155_V0.5440.73
126_K223_T0.5420.73
237_G245_A0.5420.73
55_E71_K0.5410.73
217_R222_D0.5410.73
249_P258_Y0.5380.72
197_N318_T0.5380.72
250_A261_I0.5350.72
99_C102_T0.5340.72
252_A255_D0.5340.72
79_G114_M0.5320.72
111_T177_L0.5320.72
32_G35_I0.5300.71
93_K234_P0.5300.71
64_L68_A0.5250.71
108_T151_L0.5250.71
83_M117_T0.5250.71
78_I84_N0.5250.71
226_D229_K0.5240.71
183_T322_Q0.5240.71
62_P65_K0.5240.71
276_F311_Y0.5230.70
39_G142_S0.5220.70
242_Y329_Q0.5220.70
240_Y322_Q0.5220.70
106_V134_V0.5220.70
314_V345_F0.5210.70
118_A133_M0.5210.70
26_I70_V0.5190.70
238_I325_R0.5190.70
156_V160_D0.5140.69
282_A289_V0.5140.69
67_I156_V0.5140.69
172_M192_T0.5100.68
52_V62_P0.5090.68
88_W109_H0.5090.68
108_T147_G0.5080.68
341_I345_F0.5080.68
323_K326_V0.5050.68
143_M174_D0.5030.67
337_D343_Q0.5020.67
258_Y261_I0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1wsaA 4 0.9138 100 0.078 Contact Map
2wltA 4 0.9253 100 0.08 Contact Map
1nnsA 4 0.931 100 0.08 Contact Map
3nxkA 4 0.9339 100 0.082 Contact Map
4pgaA 4 0.9368 100 0.086 Contact Map
1agxA 4 0.9282 100 0.088 Contact Map
1o7jA 4 0.9253 100 0.091 Contact Map
2d6fA 2 0.9626 100 0.099 Contact Map
1zq1A 2 0.9799 100 0.1 Contact Map
1wlsA 2 0.8966 100 0.105 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0092 seconds.