GREMLIN Database
LEP - Signal peptidase I
UniProt: P00803 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10530
Length: 324 (271)
Sequences: 932 (726)
Seq/√Len: 44.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
243_I255_P4.9911.00
145_I297_A4.3441.00
78_R83_E3.8211.00
132_V271_M3.5031.00
251_Y258_Q3.4951.00
89_S144_Y2.8991.00
104_V133_V2.8311.00
236_H239_L2.7961.00
128_R151_L2.7941.00
87_I96_L2.7801.00
232_G236_H2.6121.00
267_G290_E2.4671.00
219_E232_G2.2901.00
149_V165_L2.1531.00
148_A271_M1.9950.99
219_E224_L1.9790.99
255_P265_P1.9600.99
105_E108_A1.9270.99
66_S70_V1.9040.99
232_G239_L1.8980.99
89_S98_I1.8820.99
229_E232_G1.8770.99
178_E181_L1.8540.99
91_S146_K1.8260.99
136_Y144_Y1.8070.98
146_K273_G1.8050.98
131_I297_A1.8010.98
113_D118_K1.7980.98
234_V239_L1.7910.98
178_E183_V1.7890.98
158_Y284_Y1.7730.98
74_V78_R1.7530.98
124_G296_R1.7460.98
219_E236_H1.7370.98
164_E171_C1.7310.98
248_V253_Q1.7210.98
92_M145_I1.7170.98
86_Q101_F1.7090.98
229_E239_L1.6870.98
108_A124_G1.6820.97
90_G94_P1.6660.97
91_S273_G1.6650.97
224_L232_G1.6620.97
58_P61_L1.6600.97
142_L280_A1.6560.97
84_P301_W1.6510.97
213_P218_K1.6400.97
67_V311_W1.6320.97
302_M320_I1.6190.97
241_V245_Q1.5960.97
82_Y107_F1.5960.97
131_I145_I1.5890.96
144_Y280_A1.5640.96
91_S279_S1.5480.96
146_K279_S1.5410.96
70_V74_V1.5320.96
243_I265_P1.5240.95
220_N231_L1.5180.95
83_E297_A1.5030.95
82_Y298_T1.5030.95
164_E174_G1.4940.95
221_G224_L1.4890.95
303_S316_R1.4870.95
225_S232_G1.4860.95
63_T66_S1.4840.95
229_E236_H1.4790.95
78_R84_P1.4750.95
230_T234_V1.4630.94
128_R154_D1.4410.94
205_A211_E1.4350.94
78_R145_I1.4230.93
212_V215_N1.4190.93
179_N183_V1.4170.93
273_G279_S1.3970.93
225_S229_E1.3940.93
222_I225_S1.3850.92
90_G98_I1.3840.92
112_K135_K1.3730.92
228_K234_V1.3680.92
228_K232_G1.3650.92
57_K61_L1.3620.92
222_I230_T1.3590.92
222_I236_H1.3540.92
212_V216_E1.3490.91
241_V254_Q1.3380.91
224_L234_V1.3370.91
315_L320_I1.3350.91
219_E225_S1.3260.91
230_T238_I1.3180.90
289_P292_N1.3000.90
221_G236_H1.2940.90
83_E92_M1.2930.90
205_A216_E1.2930.90
157_T169_P1.2900.89
219_E234_V1.2840.89
210_F216_E1.2840.89
236_H240_T1.2670.89
95_T131_I1.2580.88
63_T73_I1.2580.88
223_R226_E1.2530.88
228_K236_H1.2470.88
229_E234_V1.2460.88
92_M297_A1.2370.87
78_R297_A1.2230.87
219_E222_I1.2190.87
274_D279_S1.2090.86
61_L65_A1.2050.86
58_P62_E1.2020.86
85_F96_L1.2010.86
221_G239_L1.1980.86
83_E145_I1.1970.86
178_E185_Y1.1910.85
132_V288_V1.1910.85
247_Q252_Y1.1700.84
221_G232_G1.1650.84
272_M276_R1.1500.83
234_V238_I1.1500.83
290_E293_L1.1470.83
158_Y165_L1.1470.83
226_E229_E1.1460.83
235_T239_L1.1430.83
185_Y189_E1.1370.83
218_K225_S1.1340.82
205_A212_V1.1270.82
91_S274_D1.1220.82
225_S228_K1.1210.82
130_D296_R1.1140.81
101_F302_M1.1060.81
226_E234_V1.1060.81
219_E229_E1.0920.80
78_R92_M1.0890.80
84_P92_M1.0880.80
105_E298_T1.0850.79
224_L228_K1.0810.79
233_D236_H1.0790.79
228_K233_D1.0700.78
224_L230_T1.0620.78
309_G313_T1.0600.78
56_P61_L1.0520.77
146_K274_D1.0480.77
126_P293_L1.0480.77
159_D162_S1.0420.77
233_D237_R1.0400.76
285_W288_V1.0390.76
221_G234_V0.9980.73
225_S231_L0.9970.73
125_H173_S0.9930.73
120_L127_K0.9850.72
54_V57_K0.9820.72
73_I77_V0.9790.72
154_D286_G0.9780.72
205_A215_N0.9770.72
114_P301_W0.9750.72
71_L75_L0.9740.72
211_E216_E0.9630.71
109_Y276_R0.9590.70
226_E230_T0.9520.70
206_T211_E0.9500.70
227_R234_V0.9490.70
254_Q259_L0.9470.69
129_G270_F0.9420.69
144_Y294_V0.9390.69
165_L168_Q0.9370.69
225_S234_V0.9370.69
223_R229_E0.9260.68
221_G225_S0.9240.68
165_L272_M0.9160.67
119_T122_E0.9060.66
210_F213_P0.9040.66
77_V103_L0.9000.65
89_S96_L0.8990.65
62_E70_V0.8940.65
224_L236_H0.8930.65
154_D168_Q0.8930.65
90_G93_M0.8930.65
49_A54_V0.8930.65
133_V301_W0.8930.65
225_S236_H0.8920.65
308_E312_P0.8920.65
221_G229_E0.8910.65
211_E215_N0.8910.65
144_Y282_S0.8850.64
69_P105_E0.8840.64
53_K56_P0.8790.64
54_V58_P0.8730.63
163_K277_D0.8650.62
305_D316_R0.8620.62
154_D270_F0.8580.62
182_P185_Y0.8500.61
109_Y126_P0.8470.61
62_E74_V0.8420.60
210_F214_K0.8420.60
308_E313_T0.8410.60
253_Q258_Q0.8410.60
300_I306_K0.8400.60
192_D195_Q0.8390.60
112_K119_T0.8180.58
219_E230_T0.8130.58
49_A52_K0.8120.57
180_A184_T0.8080.57
159_D231_L0.8060.57
213_P217_T0.8040.57
111_I121_I0.8020.57
67_V73_I0.8020.57
177_C183_V0.7970.56
234_V237_R0.7970.56
159_D166_T0.7950.56
124_G127_K0.7950.56
107_F123_T0.7920.56
223_R227_R0.7890.55
234_V240_T0.7830.55
220_N226_E0.7830.55
118_K121_I0.7820.55
101_F320_I0.7810.55
65_A69_P0.7790.54
220_N224_L0.7790.54
108_A121_I0.7750.54
50_T54_V0.7730.54
150_G221_G0.7680.53
94_P152_P0.7530.52
149_V180_A0.7510.52
49_A53_K0.7490.51
224_L229_E0.7460.51
209_F212_V0.7440.51
205_A209_F0.7440.51
224_L239_L0.7430.51
151_L154_D0.7420.51
211_E214_K0.7370.50
185_Y188_V0.7350.50
230_T233_D0.7340.50
306_K313_T0.7340.50
92_M131_I0.7330.50
109_Y297_A0.7250.49
242_P300_I0.7190.49
235_T238_I0.7160.48
156_V317_L0.7130.48
241_V259_L0.7120.48
219_E235_T0.7110.48
164_E167_I0.7020.47
93_M268_Q0.7010.47
195_Q231_L0.7000.47
180_A183_V0.6960.46
114_P117_Q0.6940.46
80_F83_E0.6930.46
215_N218_K0.6900.46
85_F143_D0.6900.46
231_L234_V0.6900.46
244_A248_V0.6890.46
269_Y290_E0.6890.46
73_I76_I0.6890.46
221_G228_K0.6870.45
61_L78_R0.6850.45
52_K56_P0.6820.45
218_K226_E0.6820.45
143_D285_W0.6810.45
115_I302_M0.6790.45
307_Q314_G0.6780.45
282_S285_W0.6760.44
264_V277_D0.6740.44
222_I229_E0.6720.44
221_G238_I0.6680.44
218_K229_E0.6670.44
141_K266_P0.6670.44
105_E296_R0.6660.43
83_E136_Y0.6640.43
109_Y145_I0.6590.43
111_I122_E0.6550.42
222_I240_T0.6530.42
112_K269_Y0.6520.42
208_G212_V0.6510.42
144_Y284_Y0.6510.42
98_I272_M0.6510.42
119_T123_T0.6510.42
93_M262_W0.6500.42
121_I293_L0.6480.42
221_G230_T0.6450.42
229_E235_T0.6440.41
308_E314_G0.6440.41
307_Q313_T0.6440.41
208_G213_P0.6380.41
268_Q289_P0.6360.41
108_A112_K0.6360.41
56_P77_V0.6350.41
294_V305_D0.6350.41
92_M281_D0.6350.41
92_M147_R0.6350.41
100_D273_G0.6320.40
167_I172_S0.6320.40
120_L123_T0.6260.40
228_K237_R0.6250.40
63_T70_V0.6220.39
207_S211_E0.6220.39
51_L54_V0.6210.39
219_E239_L0.6200.39
222_I234_V0.6190.39
122_E264_V0.6170.39
56_P62_E0.6140.39
85_F294_V0.6140.39
230_T236_H0.6100.38
136_Y285_W0.6100.38
241_V255_P0.6080.38
71_L84_P0.6070.38
191_S195_Q0.6040.38
74_V89_S0.6030.38
85_F89_S0.6000.37
86_Q300_I0.5990.37
76_I131_I0.5990.37
49_A92_M0.5980.37
81_I301_W0.5980.37
200_R204_E0.5970.37
222_I232_G0.5970.37
108_A296_R0.5970.37
192_D196_T0.5960.37
282_S286_G0.5950.37
232_G237_R0.5950.37
97_L139_D0.5930.37
308_E311_W0.5900.36
177_C180_A0.5890.36
198_S212_V0.5880.36
114_P287_F0.5880.36
299_A322_G0.5880.36
304_F312_P0.5850.36
100_D274_D0.5810.36
220_N233_D0.5800.36
309_G312_P0.5800.36
197_F209_F0.5780.35
65_A70_V0.5750.35
104_V145_I0.5730.35
91_S100_D0.5730.35
228_K238_I0.5710.35
305_D308_E0.5710.35
79_S140_P0.5680.35
222_I235_T0.5650.34
182_P206_T0.5640.34
179_N185_Y0.5630.34
100_D146_K0.5620.34
146_K270_F0.5620.34
86_Q113_D0.5600.34
151_L231_L0.5600.34
136_Y284_Y0.5580.34
87_I109_Y0.5580.34
113_D117_Q0.5570.34
86_Q304_F0.5550.33
134_F285_W0.5540.33
190_P198_S0.5530.33
182_P204_E0.5510.33
277_D284_Y0.5510.33
93_M97_L0.5510.33
54_V145_I0.5500.33
222_I226_E0.5490.33
62_E78_R0.5490.33
68_F76_I0.5490.33
207_S214_K0.5480.33
150_G261_T0.5450.33
149_V156_V0.5440.32
218_K222_I0.5410.32
214_K261_T0.5410.32
103_L109_Y0.5400.32
209_F215_N0.5390.32
135_K256_G0.5390.32
168_Q263_I0.5380.32
181_L185_Y0.5380.32
57_K120_L0.5370.32
196_T199_R0.5350.32
190_P207_S0.5350.32
303_S319_R0.5320.31
262_W268_Q0.5310.31
109_Y277_D0.5300.31
168_Q171_C0.5300.31
248_V251_Y0.5300.31
55_A60_W0.5270.31
305_D309_G0.5260.31
64_G113_D0.5250.31
189_E205_A0.5250.31
126_P129_G0.5240.31
230_T235_T0.5230.31
76_I85_F0.5220.31
80_F118_K0.5210.31
83_E140_P0.5210.31
91_S270_F0.5200.30
65_A84_P0.5200.30
186_S191_S0.5190.30
65_A143_D0.5180.30
259_L299_A0.5180.30
118_K149_V0.5170.30
97_L316_R0.5170.30
93_M114_P0.5140.30
54_V92_M0.5130.30
218_K231_L0.5120.30
69_P301_W0.5120.30
193_F197_F0.5110.30
208_G214_K0.5110.30
147_R281_D0.5100.30
129_G172_S0.5100.30
228_K239_L0.5090.30
178_E186_S0.5090.30
83_E106_K0.5080.30
246_D253_Q0.5070.29
198_S211_E0.5060.29
69_P75_L0.5050.29
107_F111_I0.5050.29
95_T284_Y0.5030.29
93_M135_K0.5010.29
134_F287_F0.5000.29
155_K168_Q0.5000.29
92_M283_R0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1b12A 1 0.7623 100 0.39 Contact Map
4nv4A 2 0.3549 100 0.574 Contact Map
4me8A 1 0.3611 100 0.614 Contact Map
4n31A 5 0.4352 100 0.621 Contact Map
4k8wA 2 0.3611 100 0.665 Contact Map
1umuA 2 0.3241 97.4 0.922 Contact Map
1kcaA 6 0.2932 96.9 0.927 Contact Map
2hnfA 1 0.3858 96.5 0.931 Contact Map
3k2zA 2 0.5123 93.1 0.943 Contact Map
1jhfA 2 0.4938 84.1 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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