GREMLIN Database
PPB - Alkaline phosphatase
UniProt: P00634 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10727
Length: 471 (375)
Sequences: 2020 (1326)
Seq/√Len: 68.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
245_K250_Q4.4331.00
261_D264_S4.3871.00
287_P318_V3.6151.00
246_T249_E3.5941.00
372_Q376_E3.5871.00
122_T127_S3.5351.00
152_P160_A3.3871.00
164_A335_N3.3671.00
262_A266_N3.1381.00
262_A323_Q3.0841.00
326_D373_R2.9201.00
347_S350_K2.8901.00
128_A180_A2.7811.00
174_Q191_Y2.7801.00
171_A346_A2.6861.00
159_K223_D2.6321.00
116_G126_A2.5251.00
72_G348_I2.4971.00
177_T342_Q2.4611.00
173_L225_T2.3551.00
268_V277_L2.3061.00
172_E191_Y2.2941.00
230_A258_L2.2861.00
226_L277_L2.2181.00
327_K330_E2.1961.00
112_N441_I2.1751.00
370_A374_A2.1171.00
226_L279_G2.1041.00
141_A144_V2.1031.00
248_R258_L2.0911.00
325_T374_A2.0841.00
103_T368_D2.0771.00
155_L165_T2.0741.00
322_A369_E2.0491.00
157_M461_F2.0311.00
322_A373_R2.0301.00
138_Y141_A2.0261.00
70_L367_L2.0221.00
155_L340_F2.0201.00
169_S346_A1.9631.00
83_A364_T1.9551.00
156_E184_H1.9301.00
102_L372_Q1.9251.00
366_D369_E1.9061.00
367_L371_V1.9061.00
327_K331_L1.8931.00
80_I442_A1.8841.00
345_G367_L1.8631.00
76_G390_A1.8541.00
329_I374_A1.8391.00
330_E377_F1.8331.00
322_A366_D1.8321.00
153_T156_E1.8221.00
378_A386_V1.8091.00
68_I374_A1.7891.00
325_T343_V1.7681.00
133_T143_G1.7601.00
102_L368_D1.7441.00
154_I464_M1.7401.00
83_A365_V1.7291.00
407_G410_Q1.6760.99
303_K320_T1.6740.99
137_T141_A1.6550.99
393_A437_S1.6530.99
76_G393_A1.6420.99
373_R376_E1.6420.99
192_G232_T1.6410.99
78_S437_S1.6220.99
70_L374_A1.6160.99
71_I131_W1.5980.99
143_G180_A1.5940.99
328_A341_L1.5850.99
70_L386_V1.5670.99
215_E245_K1.5630.99
110_A455_T1.5480.99
143_G153_T1.5430.99
64_A467_A1.4800.99
112_N460_L1.4770.99
286_M324_M1.4580.99
75_M348_I1.4470.98
122_T126_A1.4380.98
379_K382_G1.4250.98
300_N320_T1.4170.98
167_N181_L1.4170.98
348_I364_T1.4150.98
166_G341_L1.3970.98
356_N359_G1.3910.98
175_D350_K1.3870.98
154_I461_F1.3820.98
185_V216_Q1.3790.98
67_I468_L1.3760.98
300_N303_K1.3740.98
415_K418_A1.3730.98
71_I177_T1.3630.98
70_L371_V1.3620.98
70_L345_G1.3520.98
142_L179_A1.3500.98
300_N366_D1.3460.98
131_W460_L1.3430.97
378_A444_Y1.3340.97
117_K138_Y1.3230.97
226_L281_F1.3230.97
79_E352_D1.3200.97
126_A139_N1.3170.97
72_G75_M1.3070.97
285_N350_K1.2970.97
288_V347_S1.2940.97
226_L268_V1.2940.97
249_E252_Q1.2920.97
143_G151_H1.2890.97
282_A287_P1.2830.97
87_A99_A1.2750.97
171_A350_K1.2730.97
168_V328_A1.2630.96
450_N466_A1.2560.96
128_A132_S1.2560.96
374_A378_A1.2500.96
110_A441_I1.2470.96
136_K456_D1.2460.96
77_D440_R1.2410.96
133_T151_H1.2400.96
191_Y285_N1.2390.96
122_T457_Q1.2370.96
268_V274_Q1.2310.96
405_A408_L1.2080.95
87_A365_V1.2070.95
387_I451_V1.1990.95
260_S264_S1.1980.95
76_G437_S1.1960.95
322_A370_A1.1910.95
158_A470_L1.1860.95
345_G370_A1.1860.95
142_L185_V1.1840.95
279_G324_M1.1810.95
129_T143_G1.1770.95
110_A452_V1.1730.94
231_K284_G1.1720.94
282_A318_V1.1710.94
172_E284_G1.1690.94
363_E366_D1.1670.94
131_W342_Q1.1660.94
459_D462_Y1.1530.94
116_G139_N1.1490.94
172_E285_N1.1430.94
75_M80_I1.1400.94
405_A409_T1.1390.94
158_A468_L1.1320.93
405_A418_A1.1320.93
125_A142_L1.1260.93
258_L280_L1.1220.93
226_L324_M1.1210.93
127_S457_Q1.1200.93
459_D463_T1.1160.93
163_L468_L1.1160.93
167_N182_V1.1150.93
69_L340_F1.1110.93
325_T329_I1.1090.93
152_P157_M1.1080.93
159_K165_T1.1080.93
375_L444_Y1.1060.93
461_F465_K1.0970.92
302_D359_G1.0930.92
277_L331_L1.0910.92
288_V351_Q1.0900.92
451_V463_T1.0890.92
234_A246_T1.0880.92
377_F381_E1.0860.92
119_D139_N1.0790.92
256_Y276_P1.0700.91
155_L222_A1.0670.91
182_V217_L1.0660.91
87_A368_D1.0660.91
223_D276_P1.0520.91
132_S181_L1.0500.90
158_A163_L1.0330.90
441_I460_L1.0330.90
418_A426_N1.0310.90
84_R438_Q1.0310.90
122_T139_N1.0310.90
345_G374_A1.0300.90
354_A431_S1.0300.90
378_A384_T1.0270.89
326_D330_E1.0270.89
285_N347_S1.0270.89
112_N455_T1.0260.89
175_D285_N1.0250.89
132_S143_G1.0250.89
65_K383_N1.0220.89
249_E253_A1.0200.89
67_I464_M1.0140.89
387_I464_M1.0130.89
117_K146_I1.0110.89
108_H448_A1.0090.89
368_D372_Q0.9980.88
116_G122_T0.9960.88
113_K454_L0.9930.88
281_F324_M0.9920.88
158_A165_T0.9910.88
320_T323_Q0.9880.88
185_V190_C0.9830.87
107_T444_Y0.9820.87
379_K444_Y0.9740.87
346_A350_K0.9730.87
365_V369_E0.9730.87
157_M465_K0.9720.87
68_I378_A0.9680.86
375_L386_V0.9670.86
163_L337_K0.9660.86
386_V444_Y0.9640.86
248_R252_Q0.9630.86
151_H184_H0.9610.86
259_V277_L0.9600.86
142_L180_A0.9570.86
288_V354_A0.9560.86
251_A278_L0.9540.86
408_L411_A0.9530.86
269_T274_Q0.9520.86
138_Y146_I0.9500.85
140_G187_S0.9490.85
145_D149_K0.9480.85
352_D360_Q0.9470.85
106_Y444_Y0.9460.85
174_Q214_T0.9450.85
226_L265_L0.9420.85
367_L388_V0.9410.85
175_D191_Y0.9410.85
174_Q232_T0.9350.85
224_V328_A0.9340.84
183_A213_I0.9310.84
214_T217_L0.9250.84
406_P409_T0.9230.84
373_R377_F0.9230.84
172_E175_D0.9210.84
218_L247_L0.9200.84
72_G390_A0.9180.83
90_A362_G0.9160.83
380_K383_N0.9110.83
190_C193_P0.9100.83
106_Y375_L0.9100.83
127_S131_W0.9100.83
108_H452_V0.9050.83
79_E393_A0.9050.83
268_V331_L0.9030.82
455_T459_D0.9000.82
230_A260_S0.8980.82
455_T463_T0.8970.82
233_F280_L0.8950.82
370_A373_R0.8930.82
69_L131_W0.8920.82
164_A223_D0.8860.81
175_D189_K0.8850.81
274_Q331_L0.8840.81
408_L412_L0.8740.80
174_Q233_F0.8710.80
155_L181_L0.8700.80
410_Q413_N0.8700.80
75_M389_T0.8620.79
167_N222_A0.8610.79
235_E243_Q0.8530.79
378_A382_G0.8520.79
420_M424_Y0.8510.78
441_I455_T0.8490.78
322_A326_D0.8470.78
153_T184_H0.8430.78
406_P410_Q0.8400.78
82_A422_M0.8390.77
265_L268_V0.8390.77
113_K456_D0.8340.77
81_T398_I0.8330.77
460_L464_M0.8310.77
72_G389_T0.8280.77
263_A337_K0.8280.77
263_A266_N0.8220.76
103_T106_Y0.8220.76
102_L375_L0.8210.76
81_T85_N0.8190.76
84_R104_G0.8120.75
269_T273_Q0.8050.75
394_H398_I0.8030.74
265_L327_K0.8030.74
281_F319_P0.8030.74
266_N327_K0.8030.74
343_V370_A0.7990.74
407_G411_A0.7990.74
443_A451_V0.7920.73
248_R260_S0.7900.73
321_L366_D0.7900.73
80_I440_R0.7870.73
389_T439_L0.7870.73
408_L413_N0.7860.73
135_V151_H0.7840.73
86_Y365_V0.7830.72
182_V225_T0.7780.72
174_Q285_N0.7760.72
77_D111_L0.7740.72
166_G224_V0.7690.71
164_A333_S0.7690.71
261_D318_V0.7690.71
275_K334_K0.7680.71
265_L281_F0.7660.71
345_G366_D0.7660.71
261_D317_S0.7650.71
418_A421_V0.7590.70
365_V368_D0.7580.70
132_S340_F0.7570.70
75_M390_A0.7570.70
374_A386_V0.7570.70
379_K383_N0.7570.70
393_A435_T0.7560.70
70_L343_V0.7550.70
75_M364_T0.7530.70
304_P317_S0.7510.69
419_V422_M0.7470.69
224_V331_L0.7460.69
112_N387_I0.7440.69
168_V224_V0.7430.68
94_F98_D0.7400.68
445_G448_A0.7390.68
329_I378_A0.7380.68
282_A286_M0.7330.67
387_I441_I0.7300.67
235_E246_T0.7290.67
443_A448_A0.7290.67
69_L389_T0.7280.67
412_L418_A0.7260.67
259_V268_V0.7240.66
69_L387_I0.7210.66
288_V350_K0.7210.66
343_V374_A0.7210.66
103_T442_A0.7180.66
355_A395_A0.7150.66
378_A381_E0.7130.65
385_L443_A0.7120.65
164_A337_K0.7110.65
266_N323_Q0.7100.65
162_G337_K0.7090.65
265_L279_G0.7080.65
111_L440_R0.7080.65
194_S213_I0.7060.65
213_I216_Q0.7060.65
175_D288_V0.7060.65
126_A187_S0.7050.64
224_V277_L0.7020.64
142_L183_A0.7010.64
89_G92_G0.7010.64
164_A334_K0.6960.63
405_A410_Q0.6950.63
259_V265_L0.6930.63
79_E360_Q0.6920.63
339_F377_F0.6910.63
345_G348_I0.6910.63
415_K420_M0.6900.63
330_E336_E0.6890.63
351_Q356_N0.6880.63
249_E263_A0.6880.63
231_K283_D0.6870.62
132_S342_Q0.6830.62
154_I465_K0.6820.62
385_L448_A0.6820.62
76_G116_G0.6790.62
70_L370_A0.6770.61
191_Y284_G0.6760.61
286_M318_V0.6750.61
171_A347_S0.6750.61
76_G396_S0.6750.61
352_D396_S0.6740.61
191_Y232_T0.6730.61
100_L104_G0.6730.61
159_K221_R0.6720.61
170_T286_M0.6710.61
243_Q246_T0.6690.60
393_A396_S0.6680.60
326_D377_F0.6670.60
419_V424_Y0.6630.60
358_C362_G0.6580.59
82_A361_I0.6550.59
175_D346_A0.6530.59
131_W177_T0.6520.58
165_T222_A0.6490.58
376_E380_K0.6490.58
333_S377_F0.6480.58
71_I342_Q0.6470.58
352_D394_H0.6460.58
369_E372_Q0.6440.57
183_A419_V0.6430.57
68_I226_L0.6410.57
118_P138_Y0.6410.57
234_A248_R0.6400.57
94_F101_P0.6400.57
353_H392_H0.6400.57
71_I367_L0.6390.57
226_L259_V0.6390.57
248_R278_L0.6390.57
385_L445_G0.6380.57
347_S363_E0.6370.57
93_F99_A0.6360.57
252_Q337_K0.6340.56
157_M470_L0.6330.56
395_A429_E0.6320.56
304_P315_N0.6310.56
424_Y427_S0.6310.56
252_Q258_L0.6290.56
131_W387_I0.6290.56
115_T456_D0.6290.56
234_A283_D0.6260.55
387_I463_T0.6260.55
86_Y361_I0.6240.55
178_P353_H0.6240.55
156_E160_A0.6210.55
175_D347_S0.6200.55
83_A101_P0.6190.55
405_A412_L0.6190.55
407_G415_K0.6180.54
246_T250_Q0.6170.54
255_G275_K0.6170.54
269_T327_K0.6170.54
329_I377_F0.6170.54
421_V424_Y0.6160.54
415_K426_N0.6150.54
442_A454_L0.6140.54
237_A244_G0.6140.54
186_T190_C0.6140.54
166_G332_L0.6110.54
172_E347_S0.6090.53
407_G412_L0.6080.53
60_S449_A0.6050.53
371_V388_V0.6030.53
301_I359_G0.6020.52
128_A181_L0.6000.52
376_E379_K0.5980.52
421_V426_N0.5970.52
222_A225_T0.5960.52
396_S399_V0.5960.52
326_D372_Q0.5960.52
55_L466_A0.5940.52
184_H216_Q0.5920.51
121_V436_G0.5900.51
128_A461_F0.5900.51
377_F380_K0.5890.51
122_T130_A0.5880.51
104_G438_Q0.5880.51
66_N224_V0.5880.51
381_E446_P0.5880.51
221_R276_P0.5860.51
415_K427_S0.5840.50
392_H434_H0.5840.50
69_L464_M0.5840.50
71_I389_T0.5800.50
323_Q326_D0.5790.50
230_A248_R0.5780.50
251_A255_G0.5770.49
387_I461_F0.5760.49
224_V341_L0.5750.49
346_A351_Q0.5730.49
173_L280_L0.5730.49
409_T413_N0.5730.49
64_A383_N0.5730.49
68_I384_T0.5720.49
105_Q334_K0.5720.49
253_A263_A0.5710.49
351_Q359_G0.5690.49
121_V400_A0.5680.48
168_V341_L0.5680.48
133_T153_T0.5670.48
129_T137_T0.5660.48
178_P392_H0.5650.48
418_A422_M0.5650.48
352_D424_Y0.5650.48
129_T363_E0.5650.48
72_G388_V0.5640.48
92_G325_T0.5640.48
170_T324_M0.5640.48
304_P316_D0.5630.48
381_E386_V0.5630.48
214_T247_L0.5610.48
279_G319_P0.5600.47
110_A453_G0.5590.47
163_L470_L0.5590.47
353_H434_H0.5590.47
64_A446_P0.5580.47
187_S190_C0.5570.47
452_V455_T0.5570.47
389_T441_I0.5560.47
70_L329_I0.5560.47
175_D351_Q0.5550.47
129_T461_F0.5540.47
148_E186_T0.5530.47
131_W461_F0.5530.47
247_L250_Q0.5520.46
60_S447_H0.5520.46
88_E94_F0.5510.46
91_G99_A0.5470.46
71_I128_A0.5450.46
59_L62_K0.5450.46
132_S153_T0.5430.45
409_T412_L0.5420.45
240_G260_S0.5420.45
399_V423_S0.5410.45
81_T438_Q0.5390.45
218_L250_Q0.5390.45
169_S173_L0.5380.45
405_A411_A0.5370.45
450_N463_T0.5370.45
359_G362_G0.5370.45
257_Q268_V0.5360.45
170_T347_S0.5350.44
339_F378_A0.5340.44
380_K444_Y0.5340.44
159_K220_A0.5330.44
86_Y362_G0.5330.44
107_T452_V0.5330.44
265_L319_P0.5330.44
405_A415_K0.5320.44
412_L415_K0.5310.44
276_P334_K0.5300.44
88_E97_I0.5300.44
318_V324_M0.5290.44
86_Y358_C0.5290.44
101_P364_T0.5280.44
263_A267_S0.5260.43
88_E92_G0.5260.43
126_A137_T0.5260.43
173_L217_L0.5250.43
126_A140_G0.5240.43
142_L145_D0.5230.43
224_V279_G0.5230.43
329_I339_F0.5210.43
419_V423_S0.5190.43
100_L105_Q0.5190.43
114_K439_L0.5190.43
171_A175_D0.5180.42
230_A283_D0.5170.42
413_N418_A0.5170.42
64_A163_L0.5170.42
258_L323_Q0.5160.42
400_A433_E0.5160.42
103_T371_V0.5150.42
180_A342_Q0.5150.42
77_D80_I0.5150.42
71_I132_S0.5150.42
78_S396_S0.5120.42
355_A429_E0.5120.42
375_L383_N0.5110.42
150_D185_V0.5110.42
155_L185_V0.5110.42
190_C216_Q0.5100.42
252_Q255_G0.5090.41
154_I158_A0.5080.41
417_G423_S0.5080.41
127_S394_H0.5080.41
69_L181_L0.5070.41
228_G286_M0.5060.41
325_T370_A0.5060.41
169_S342_Q0.5040.41
420_M426_N0.5030.41
360_Q400_A0.5030.41
155_L158_A0.5020.41
390_A439_L0.5020.41
393_A397_Q0.5000.40
369_E373_R0.5000.40
390_A394_H0.5000.40
334_K337_K0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3e2dA 2 0.7856 100 0.204 Contact Map
1k7hA 2 0.862 100 0.25 Contact Map
1zedA 2 0.8747 100 0.34 Contact Map
2x98A 4 0.7749 100 0.367 Contact Map
3wbhA 2 0.7834 100 0.421 Contact Map
3a52A 2 0.7367 100 0.441 Contact Map
3tg0A 2 0.9172 100 0.462 Contact Map
2w5vA 2 0.7282 100 0.667 Contact Map
2mlyB 1 0.2781 100 0.779 Contact Map
3q3qA 2 0.7431 99.9 0.835 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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