GREMLIN Database
DHE4 - NADP-specific glutamate dehydrogenase
UniProt: P00370 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10372
Length: 447 (403)
Sequences: 4726 (2276)
Seq/√Len: 113.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
251_A256_A4.4391.00
236_S315_V3.6411.00
336_I358_L3.3521.00
58_E86_S3.2431.00
219_T344_A3.2221.00
237_V248_I2.9261.00
275_T278_K2.9241.00
124_M381_G2.9171.00
312_S334_Q2.7491.00
281_R289_R2.7431.00
248_I261_A2.6851.00
228_M233_M2.6531.00
359_F364_V2.6201.00
141_G145_R2.5801.00
221_A224_K2.5731.00
360_Q424_N2.4631.00
234_R255_G2.4491.00
357_E361_Q2.4121.00
221_A407_L2.4081.00
152_T182_M2.3361.00
327_L351_T2.3311.00
234_R257_R2.3221.00
62_Q103_L2.3161.00
69_D75_Q2.2911.00
217_Y220_E2.2611.00
235_V256_A2.2581.00
225_R414_V2.2341.00
235_V251_A2.1921.00
206_I395_A2.1721.00
84_F110_G2.1671.00
260_T310_P2.1621.00
220_E403_H2.1461.00
66_V149_A2.1411.00
329_V354_E2.1371.00
410_H414_V2.1291.00
172_G175_E2.1261.00
134_D137_G2.1161.00
397_K401_R2.0261.00
200_S439_D2.0201.00
351_T359_F2.0031.00
424_N427_Q1.9901.00
133_F138_K1.9441.00
314_P336_I1.9191.00
225_R410_H1.9011.00
95_M179_M1.8731.00
306_E309_Q1.8651.00
230_F256_A1.8531.00
152_T186_L1.8351.00
215_L346_G1.8151.00
221_A411_H1.8141.00
320_P347_A1.7801.00
229_G232_G1.7801.00
169_I323_T1.7781.00
260_T313_L1.7651.00
332_A358_L1.7641.00
151_M179_M1.7641.00
155_Y187_S1.7401.00
70_D139_S1.7371.00
271_E303_V1.7321.00
79_A150_L1.7291.00
249_E253_E1.7251.00
152_T185_K1.6721.00
236_S260_T1.6701.00
418_G422_Q1.6561.00
133_F146_F1.6551.00
102_N105_I1.6511.00
278_K281_R1.6481.00
37_P40_E1.6451.00
39_L43_P1.6301.00
403_H407_L1.6151.00
344_A372_A1.6081.00
327_L335_L1.5851.00
276_K280_A1.5641.00
217_Y399_D1.5481.00
136_K139_S1.5481.00
265_S292_R1.5401.00
411_H415_E1.5401.00
328_D331_A1.5391.00
267_T305_L1.5331.00
215_L344_A1.5311.00
367_A372_A1.5201.00
49_S73_Q1.5091.00
235_V319_L1.5011.00
344_A367_A1.4981.00
60_V107_K1.4981.00
261_A279_L1.4961.00
173_G208_P1.4951.00
144_M148_Q1.4921.00
182_M185_K1.4861.00
347_A350_P1.4821.00
358_L362_A1.4811.00
274_F279_L1.4791.00
278_K296_Y1.4631.00
247_A321_C1.4581.00
329_V358_L1.4361.00
435_V439_D1.4351.00
82_V110_G1.4311.00
68_V141_G1.4251.00
249_E280_A1.4211.00
155_Y186_L1.4201.00
140_E174_R1.4131.00
148_Q182_M1.4121.00
259_I315_V1.4111.00
38_F42_N1.4061.00
42_N45_Y1.4011.00
394_K397_K1.4001.00
421_E427_Q1.3941.00
243_V346_G1.3941.00
228_M341_K1.3911.00
86_S91_Y1.3761.00
95_M150_L1.3521.00
220_E224_K1.3471.00
251_A258_V1.3341.00
139_S142_E1.3311.00
333_H358_L1.3171.00
410_H425_Y1.3161.00
225_R418_G1.3151.00
48_M442_L1.3121.00
216_V251_A1.3101.00
88_I121_T1.3071.00
267_T307_G1.3010.99
259_I269_V1.2990.99
294_A298_K1.2990.99
60_V103_L1.2960.99
200_S443_A1.2940.99
264_S283_I1.2860.99
236_S259_I1.2810.99
247_A319_L1.2740.99
56_E78_R1.2690.99
237_V256_A1.2630.99
327_L359_F1.2610.99
222_M367_A1.2570.99
387_N392_G1.2540.99
329_V333_H1.2540.99
394_K404_H1.2390.99
65_V149_A1.2350.99
304_Y307_G1.2350.99
45_Y439_D1.2330.99
80_W132_D1.2280.99
39_L42_N1.2270.99
138_K146_F1.2250.99
219_T317_I1.2250.99
149_A152_T1.2220.99
114_T437_V1.2210.99
278_K300_F1.2130.99
235_V258_V1.2090.99
414_V418_G1.2050.99
270_D296_Y1.1990.99
234_R316_D1.1980.99
423_T431_I1.1980.99
97_F171_V1.1970.99
215_L319_L1.1800.99
37_P41_Q1.1800.99
82_V103_L1.1790.99
235_V317_I1.1740.99
63_F154_L1.1700.99
236_S313_L1.1610.99
149_A153_E1.1570.99
208_P246_Y1.1480.99
85_S441_M1.1460.99
234_R271_E1.1460.99
115_F437_V1.1460.99
220_E254_F1.1390.99
35_L38_F1.1320.99
225_R411_H1.1320.99
252_M277_E1.1300.99
215_L321_C1.1300.99
226_H365_L1.1280.99
282_L290_D1.1270.99
378_A382_L1.1250.99
41_Q44_K1.1210.98
291_G295_D1.1210.98
40_E44_K1.1110.98
215_L219_T1.1110.98
279_L282_L1.1080.98
220_E230_F1.1050.98
217_Y403_H1.1020.98
356_T360_Q1.0980.98
252_M276_K1.0980.98
257_R272_S1.0960.98
351_T355_A1.0940.98
270_D278_K1.0930.98
279_L283_I1.0880.98
38_F41_Q1.0850.98
308_Q311_W1.0810.98
219_T319_L1.0800.98
162_T188_N1.0740.98
416_H432_A1.0720.98
282_L296_Y1.0720.98
282_L291_G1.0680.98
222_M344_A1.0610.98
318_A335_L1.0600.98
120_T374_A1.0580.98
52_E55_V1.0540.98
278_K282_L1.0510.98
245_Q280_A1.0490.98
439_D442_L1.0480.98
40_E43_P1.0460.98
319_L344_A1.0450.98
139_S143_V1.0430.98
356_T426_V1.0430.98
104_S107_K1.0370.97
115_F370_K1.0360.97
399_D403_H1.0350.97
179_M182_M1.0320.97
182_M186_L1.0280.97
97_F147_C1.0260.97
35_L39_L1.0260.97
263_D292_R1.0240.97
37_P42_N1.0230.97
61_I157_H1.0220.97
415_E420_G1.0200.97
408_D412_A1.0190.97
342_A365_L1.0120.97
40_E45_Y0.9970.97
91_Y127_G0.9960.97
371_A413_C0.9950.97
420_G423_T0.9890.97
213_Y249_E0.9850.97
63_F79_A0.9800.96
99_P134_D0.9770.96
140_E143_V0.9760.96
288_S291_G0.9760.96
266_G294_A0.9740.96
144_M179_M0.9730.96
98_H323_T0.9710.96
201_F443_A0.9670.96
100_S105_I0.9610.96
120_T378_A0.9610.96
78_R132_D0.9550.96
66_V76_V0.9540.96
336_I362_A0.9530.96
147_C151_M0.9520.96
416_H431_I0.9510.96
415_E418_G0.9460.96
416_H421_E0.9460.96
297_A302_L0.9390.95
180_A190_T0.9390.95
136_K143_V0.9380.95
149_A185_K0.9360.95
268_V293_V0.9360.95
154_L157_H0.9330.95
251_A319_L0.9240.95
290_D293_V0.9230.95
333_H337_A0.9190.95
290_D295_D0.9160.95
218_F413_C0.9140.95
421_E424_N0.9140.95
378_A406_M0.9130.95
49_S52_E0.9120.95
282_L295_D0.9100.95
128_K348_N0.9100.95
309_Q312_S0.9080.95
308_Q312_S0.9080.95
215_L247_A0.9060.95
52_E75_Q0.9050.94
88_I125_G0.9010.94
311_W318_A0.8990.94
404_H408_D0.8980.94
434_F437_V0.8980.94
75_Q137_G0.8970.94
38_F44_K0.8970.94
269_V313_L0.8960.94
60_V82_V0.8950.94
337_A352_T0.8930.94
400_A403_H0.8910.94
37_P45_Y0.8900.94
257_R275_T0.8900.94
136_K140_E0.8880.94
343_V355_A0.8820.94
247_A251_A0.8780.94
153_E156_R0.8770.94
216_V254_F0.8740.93
136_K264_S0.8720.93
68_V75_Q0.8690.93
252_M275_T0.8650.93
351_T366_F0.8650.93
38_F43_P0.8640.93
400_A404_H0.8640.93
374_A378_A0.8620.93
269_V303_V0.8590.93
416_H435_V0.8560.93
40_E46_R0.8520.93
62_Q78_R0.8510.92
60_V106_L0.8500.92
213_Y399_D0.8490.92
69_D77_N0.8470.92
327_L369_G0.8470.92
292_R295_D0.8460.92
346_G372_A0.8450.92
79_A142_E0.8430.92
226_H341_K0.8400.92
263_D266_G0.8380.92
218_F372_A0.8360.92
319_L346_G0.8340.92
244_A263_D0.8330.92
50_L442_L0.8320.92
391_L397_K0.8310.92
273_G282_L0.8310.92
391_L394_K0.8310.92
234_R314_P0.8300.92
151_M194_F0.8280.91
54_L115_F0.8260.91
407_L411_H0.8220.91
359_F366_F0.8210.91
321_C346_G0.8170.91
316_D341_K0.8170.91
121_T405_I0.8160.91
223_L230_F0.8150.91
149_A182_M0.8150.91
312_S331_A0.8140.91
273_G279_L0.8140.91
275_T283_I0.8130.91
34_T38_F0.8120.91
237_V261_A0.8120.91
110_G114_T0.8080.90
39_L45_Y0.8040.90
282_L297_A0.8020.90
328_D352_T0.8000.90
213_Y250_K0.8000.90
222_M365_L0.7970.90
177_G198_G0.7920.90
222_M342_A0.7920.90
330_D333_H0.7890.89
278_K283_I0.7880.89
176_V201_F0.7870.89
156_R188_N0.7860.89
396_E400_A0.7830.89
198_G201_F0.7820.89
36_W40_E0.7810.89
422_Q427_Q0.7790.89
98_H169_I0.7740.88
377_V380_S0.7720.88
240_S347_A0.7710.88
276_K279_L0.7690.88
408_D411_H0.7690.88
219_T342_A0.7670.88
343_V351_T0.7670.88
414_V417_G0.7650.88
273_G278_K0.7650.88
215_L372_A0.7630.88
385_A391_L0.7630.88
251_A317_I0.7600.87
309_Q331_A0.7540.87
248_I279_L0.7510.87
138_K143_V0.7470.87
223_L342_A0.7430.86
300_F305_L0.7400.86
279_L296_Y0.7380.86
386_Q393_W0.7380.86
240_S276_K0.7360.86
267_T312_S0.7360.86
84_F114_T0.7340.86
70_D145_R0.7310.85
273_G281_R0.7310.85
246_Y249_E0.7310.85
218_F371_A0.7300.85
300_F303_V0.7290.85
147_C175_E0.7270.85
336_I364_V0.7260.85
272_S301_G0.7260.85
353_I356_T0.7250.85
308_Q330_D0.7220.85
366_F369_G0.7210.85
275_T279_L0.7210.85
88_I118_A0.7210.85
174_R177_G0.7190.84
223_L317_I0.7180.84
250_K399_D0.7160.84
61_I154_L0.7160.84
97_F131_S0.7150.84
160_A190_T0.7140.84
39_L46_R0.7130.84
209_E246_Y0.7120.84
43_P46_R0.7110.84
276_K283_I0.7100.84
158_L179_M0.7100.84
65_V146_F0.7080.84
439_D443_A0.7080.84
248_I276_K0.7080.84
59_R83_Q0.7050.83
160_A189_N0.7030.83
39_L44_K0.7010.83
38_F45_Y0.7000.83
274_F282_L0.6990.83
267_T313_L0.6980.83
335_L359_F0.6950.82
395_A399_D0.6950.82
265_S304_Y0.6940.82
116_K348_N0.6910.82
106_L110_G0.6820.81
195_T380_S0.6810.81
230_F235_V0.6790.81
62_Q80_W0.6790.81
351_T356_T0.6790.81
213_Y411_H0.6780.81
261_A293_V0.6760.81
166_A377_V0.6750.81
366_F426_V0.6740.80
121_T408_D0.6740.80
409_I413_C0.6730.80
67_W77_N0.6730.80
268_V279_L0.6700.80
75_Q138_K0.6690.80
35_L41_Q0.6690.80
343_V359_F0.6680.80
34_T39_L0.6680.80
170_G243_V0.6670.80
254_F279_L0.6670.80
249_E277_E0.6660.80
161_D388_A0.6660.80
260_T267_T0.6650.80
135_P143_V0.6610.79
314_P337_A0.6610.79
356_T359_F0.6570.79
357_E360_Q0.6560.79
79_A145_R0.6560.79
246_Y250_K0.6560.79
417_G423_T0.6540.78
138_K142_E0.6530.78
95_M165_P0.6530.78
184_K444_Q0.6520.78
82_V127_G0.6520.78
63_F67_W0.6520.78
129_G150_L0.6520.78
36_W39_L0.6510.78
385_A389_A0.6500.78
143_V290_D0.6500.78
236_S267_T0.6480.78
417_G425_Y0.6460.78
160_A191_A0.6430.77
306_E310_P0.6390.77
112_E349_M0.6390.77
281_R288_S0.6360.76
328_D334_Q0.6350.76
234_R269_V0.6330.76
80_W150_L0.6330.76
295_D304_Y0.6320.76
243_V264_S0.6320.76
88_I436_K0.6320.76
328_D351_T0.6290.76
240_S320_P0.6280.76
416_H423_T0.6280.76
422_Q426_V0.6270.75
333_H362_A0.6260.75
68_V74_I0.6260.75
423_T427_Q0.6260.75
379_T398_V0.6250.75
212_G247_A0.6250.75
148_Q181_G0.6220.75
412_A415_E0.6220.75
145_R148_Q0.6200.75
185_K192_C0.6190.75
250_K256_A0.6180.74
69_D89_G0.6180.74
122_L437_V0.6170.74
211_T242_N0.6170.74
212_G243_V0.6150.74
42_N46_R0.6150.74
80_W149_A0.6150.74
45_Y431_I0.6150.74
274_F278_K0.6150.74
262_S310_P0.6130.74
317_I342_A0.6100.73
211_T373_N0.6100.73
204_S398_V0.6090.73
216_V219_T0.6070.73
119_L412_A0.6070.73
108_F349_M0.6060.73
176_V194_F0.6050.73
400_A407_L0.6050.73
87_A437_V0.6040.73
296_Y302_L0.6040.73
433_G437_V0.6020.73
229_G233_M0.6020.73
221_A225_R0.6020.73
34_T37_P0.6020.73
225_R365_L0.6010.72
124_M384_M0.5990.72
365_L425_Y0.5990.72
375_G379_T0.5990.72
327_L366_F0.5990.72
318_A343_V0.5970.72
52_E153_E0.5940.72
233_M316_D0.5920.71
276_K282_L0.5920.71
224_K407_L0.5920.71
120_T409_I0.5910.71
289_R295_D0.5870.71
37_P44_K0.5870.71
83_Q162_T0.5870.71
302_L306_E0.5840.70
360_Q366_F0.5830.70
86_S184_K0.5830.70
40_E55_V0.5820.70
44_K51_L0.5820.70
128_K166_A0.5810.70
204_S386_Q0.5810.70
45_Y435_V0.5800.70
367_A425_Y0.5800.70
323_T326_E0.5800.70
247_A346_G0.5780.69
198_G443_A0.5760.69
327_L343_V0.5760.69
226_H342_A0.5740.69
277_E280_A0.5720.69
43_P51_L0.5710.69
270_D301_G0.5700.68
133_F139_S0.5690.68
267_T310_P0.5690.68
412_A416_H0.5670.68
331_A358_L0.5660.68
302_L305_L0.5640.68
271_E297_A0.5630.67
83_Q91_Y0.5610.67
153_E187_S0.5610.67
121_T412_A0.5600.67
434_F438_A0.5590.67
118_A433_G0.5590.67
128_K211_T0.5590.67
266_G307_G0.5580.67
137_G140_E0.5580.67
307_G310_P0.5580.67
303_V306_E0.5570.67
378_A405_I0.5570.67
237_V251_A0.5560.67
343_V364_V0.5560.67
335_L343_V0.5560.67
228_M342_A0.5550.66
147_C179_M0.5540.66
363_G422_Q0.5510.66
220_E231_E0.5500.66
356_T366_F0.5490.66
116_K128_K0.5490.66
180_A194_F0.5480.65
87_A441_M0.5480.65
179_M194_F0.5470.65
402_L406_M0.5470.65
373_N377_V0.5420.65
101_V130_G0.5420.65
92_K380_S0.5420.65
245_Q276_K0.5410.64
180_A184_K0.5410.64
320_P327_L0.5400.64
216_V250_K0.5400.64
128_K373_N0.5390.64
297_A301_G0.5380.64
79_A149_A0.5370.64
111_F115_F0.5360.64
80_W133_F0.5360.64
206_I379_T0.5360.64
261_A282_L0.5360.64
200_S440_A0.5360.64
270_D274_F0.5360.64
417_G421_E0.5340.63
270_D302_L0.5320.63
69_D73_Q0.5310.63
99_P136_K0.5310.63
305_L313_L0.5300.63
295_D298_K0.5300.63
125_G375_G0.5300.63
128_K242_N0.5300.63
222_M410_H0.5290.63
236_S310_P0.5290.63
195_T207_R0.5280.63
398_V403_H0.5280.63
50_L438_A0.5260.62
212_G346_G0.5260.62
89_G158_L0.5250.62
314_P341_K0.5250.62
40_E47_Q0.5250.62
220_E407_L0.5240.62
411_H414_V0.5220.62
291_G294_A0.5220.62
410_H413_C0.5210.62
380_S383_E0.5200.61
92_K207_R0.5200.61
161_D390_R0.5200.61
145_R152_T0.5180.61
327_L331_A0.5170.61
423_T428_G0.5170.61
248_I252_M0.5160.61
68_V144_M0.5130.60
213_Y253_E0.5130.60
280_A283_I0.5130.60
266_G293_V0.5110.60
236_S314_P0.5110.60
64_R68_V0.5100.60
421_E425_Y0.5090.60
157_H162_T0.5090.60
435_V442_L0.5070.59
273_G283_I0.5070.59
183_M187_S0.5070.59
290_D294_A0.5060.59
117_N377_V0.5060.59
151_M183_M0.5060.59
230_F233_M0.5060.59
240_S293_V0.5050.59
170_G212_G0.5050.59
312_S335_L0.5050.59
150_L401_R0.5040.59
106_L130_G0.5010.59
246_Y288_S0.5010.59
433_G440_A0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3r3jA 5 0.9933 100 0.093 Contact Map
4fccA 5 0.9799 100 0.093 Contact Map
2bmaA 5 0.9933 100 0.096 Contact Map
1bgvA 5 0.9843 100 0.1 Contact Map
3mw9A 5 0.9396 100 0.139 Contact Map
4xgiA 6 0.9217 100 0.14 Contact Map
2yfqA 5 0.868 100 0.141 Contact Map
3aoeE 3 0.9284 100 0.144 Contact Map
2tmgA 5 0.9083 100 0.144 Contact Map
1v9lA 4 0.9195 100 0.155 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0115 seconds.