GREMLIN Database
ISPF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
UniProt: Q06756 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 158 (155)
Sequences: 3001 (1709)
Seq/√Len: 137.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
18_P25_E3.1071.00
75_F120_G2.6641.00
34_G38_A2.6061.00
14_V33_L2.3801.00
80_H83_G2.3771.00
42_L117_I2.2901.00
41_L97_C2.2551.00
6_Q150_Q2.2261.00
45_V95_I2.1971.00
22_G41_L2.1861.00
49_C85_V2.1751.00
82_W121_L2.0281.00
10_V138_L2.0141.00
103_K144_A1.9261.00
46_A81_V1.8911.00
45_V97_C1.8521.00
80_H84_I1.7921.00
141_T148_A1.7561.00
100_I150_Q1.7561.00
123_A127_Q1.7421.00
135_T141_T1.7361.00
60_K110_I1.6751.00
93_G156_Q1.6531.00
99_I106_M1.6171.00
88_K126_S1.5951.00
101_A147_I1.5491.00
86_K122_E1.5341.00
42_L46_A1.5251.00
116_R119_E1.5221.00
75_F116_R1.5071.00
53_V85_V1.5011.00
4_I154_L1.4951.00
67_P70_K1.4881.00
72_A77_L1.4761.00
32_L38_A1.4441.00
55_E88_K1.4431.00
46_A77_L1.4401.00
12_Q33_L1.4361.00
6_Q98_T1.4281.00
82_W92_L1.3931.00
69_F72_A1.3911.00
82_W86_K1.3751.00
53_V155_I1.3751.00
8_F135_T1.3741.00
100_I141_T1.3721.00
94_N154_L1.3651.00
79_Q120_G1.3571.00
56_G60_K1.3531.00
41_L99_I1.3511.00
76_K79_Q1.3471.00
22_G113_M1.3401.00
28_Y144_A1.3251.00
96_D131_K1.3131.00
85_V155_I1.3091.00
92_L123_A1.3071.00
124_D127_Q1.2951.00
15_E30_K1.2891.00
51_G96_D1.2771.00
93_G154_L1.2711.00
114_R125_V1.2541.00
115_K125_V1.2481.00
109_Y112_D1.2321.00
115_K119_E1.2291.00
117_I130_V1.2111.00
20_I25_E1.1991.00
6_Q152_T1.1891.00
92_L95_I1.1690.99
61_H84_I1.1620.99
18_P31_G1.1550.99
140_F146_G1.1510.99
54_G129_N1.1390.99
21_I106_M1.1130.99
91_V156_Q1.1120.99
86_K91_V1.0950.99
50_L81_V1.0950.99
51_G131_K1.0920.99
107_L111_E1.0890.99
111_E114_R1.0890.99
83_G87_Q1.0880.99
64_D102_Q1.0840.99
69_F77_L1.0760.99
68_E80_H1.0710.99
60_K107_L1.0690.99
106_M110_I1.0640.99
44_T151_A1.0610.99
10_V135_T1.0590.99
111_E125_V1.0530.99
72_A76_K1.0520.99
26_I109_Y1.0500.99
42_L75_F1.0370.99
102_Q135_T1.0290.99
111_E115_K1.0260.99
52_A155_I1.0200.99
102_Q137_K0.9900.98
112_D115_K0.9860.98
21_I40_V0.9830.98
8_F148_A0.9820.98
48_A151_A0.9810.98
32_L101_A0.9800.98
14_V17_R0.9650.98
45_V117_I0.9630.98
29_E103_K0.9510.98
105_K108_P0.9420.98
47_D58_I0.9260.97
7_G47_D0.9210.97
46_A72_A0.9060.97
95_I121_L0.9040.97
8_F100_I0.9000.97
2_F93_G0.8960.97
101_A106_M0.8920.97
100_I148_A0.8870.96
17_R33_L0.8540.96
49_C155_I0.8530.96
42_L120_G0.8500.95
17_R31_G0.8460.95
46_A58_I0.8450.95
138_L146_G0.8420.95
116_R120_G0.8370.95
30_K144_A0.8370.95
90_Y155_I0.8340.95
106_M132_A0.8300.95
62_F80_H0.8260.95
48_A153_V0.8150.94
139_G146_G0.8150.94
95_I153_V0.8140.94
32_L147_I0.8070.94
55_E84_I0.8040.94
54_G114_R0.8040.94
29_E144_A0.7970.94
137_K144_A0.7910.93
110_I114_R0.7780.93
75_F79_Q0.7780.93
21_I147_I0.7650.92
100_I135_T0.7540.92
77_L81_V0.7520.92
56_G61_H0.7490.91
64_D136_E0.7450.91
19_L147_I0.7360.91
82_W123_A0.7350.91
64_D69_F0.7230.90
76_K80_H0.7220.90
135_T140_F0.7170.89
135_T143_R0.7150.89
133_T146_G0.7010.88
20_I116_R0.6990.88
40_V147_I0.6980.88
135_T148_A0.6980.88
10_V146_G0.6960.88
117_I128_V0.6960.88
22_G99_I0.6950.88
10_V140_F0.6950.88
47_D133_T0.6930.88
60_K114_R0.6830.87
84_I88_K0.6820.87
27_P109_Y0.6820.87
12_Q137_K0.6800.87
17_R38_A0.6790.87
106_M109_Y0.6790.87
82_W122_E0.6720.86
95_I123_A0.6710.86
91_V127_Q0.6700.86
17_R71_D0.6650.86
40_V149_A0.6590.85
13_L32_L0.6580.85
108_P111_E0.6570.85
2_F154_L0.6520.85
10_V141_T0.6420.84
14_V30_K0.6420.84
99_I149_A0.6400.83
84_I87_Q0.6390.83
79_Q122_E0.6360.83
8_F150_Q0.6340.83
23_G117_I0.6230.82
13_L30_K0.6200.81
19_L32_L0.6200.81
93_G123_A0.6140.81
107_L110_I0.6130.81
112_D116_R0.6130.81
113_M116_R0.6110.81
22_G97_C0.6110.81
8_F140_F0.6100.80
80_H87_Q0.6060.80
135_T138_L0.6060.80
82_W95_I0.6040.80
49_C53_V0.6030.80
98_T129_N0.6020.80
2_F156_Q0.5950.79
54_G94_N0.5930.79
33_L64_D0.5910.78
101_A141_T0.5850.78
4_I152_T0.5770.77
85_V90_Y0.5750.76
67_P71_D0.5730.76
19_L31_G0.5710.76
113_M130_V0.5690.76
78_L120_G0.5660.75
17_R20_I0.5640.75
92_L128_V0.5630.75
20_I31_G0.5620.75
13_L17_R0.5520.73
61_H129_N0.5500.73
137_K143_R0.5470.73
66_D69_F0.5440.72
7_G58_I0.5440.72
5_G52_A0.5440.72
102_Q142_G0.5410.72
53_V88_K0.5370.71
73_D77_L0.5320.71
20_I38_A0.5310.71
82_W117_I0.5260.70
139_G142_G0.5260.70
73_D76_K0.5240.70
117_I121_L0.5240.70
110_I113_M0.5220.69
68_E119_E0.5210.69
13_L147_I0.5200.69
24_I75_F0.5180.69
17_R25_E0.5180.69
98_T150_Q0.5160.68
61_H81_V0.5130.68
83_G86_K0.5110.68
41_L45_V0.5110.68
12_Q138_L0.5090.67
10_V139_G0.5090.67
60_K129_N0.5080.67
118_A128_V0.5050.67
99_I147_I0.5040.67
7_G10_V0.5030.66
49_C92_L0.5020.66
13_L146_G0.5020.66
141_T146_G0.5010.66
10_V133_T0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4c8eA 3 1 100 0.014 Contact Map
3f0dA 3 0.9873 100 0.015 Contact Map
1t0aA 3 1 100 0.017 Contact Map
3re3A 5 0.9241 100 0.018 Contact Map
1yqnA 3 0.9937 100 0.019 Contact Map
2pmpA 3 1 100 0.02 Contact Map
5b8fA 3 1 100 0.024 Contact Map
2uzhA 3 0.981 100 0.04 Contact Map
4c81A 3 0.9367 100 0.041 Contact Map
3b6nA 3 0.8924 100 0.052 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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