GREMLIN Database
CWLC - Sporulation-specific N-acetylmuramoyl-L-alanine amidase
UniProt: Q06320 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 255 (220)
Sequences: 978 (794)
Seq/√Len: 53.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
107_I138_L4.4451.00
31_I142_N3.3241.00
6_I32_A3.2211.00
76_L171_L2.9271.00
34_A161_Q2.9031.00
186_Y191_G2.8811.00
36_R50_L2.8021.00
30_Q160_I2.7821.00
32_A78_I2.7341.00
28_T80_V2.6761.00
23_Q26_T2.5691.00
81_N89_E2.5331.00
75_F129_V2.4401.00
3_K47_S2.4231.00
8_P32_A2.2901.00
89_E123_K2.2531.00
188_V191_G2.2411.00
36_R40_T2.2271.00
64_T128_H2.2041.00
82_S146_D2.1621.00
110_E173_Q2.1291.00
17_A145_I2.1001.00
5_F51_S2.0601.00
140_T167_H2.0291.00
19_G151_A2.0211.00
65_N69_N1.9851.00
59_S62_D1.9281.00
193_F199_A1.9101.00
108_H119_D1.8670.99
22_L151_A1.8360.99
189_Q192_A1.8190.99
25_K55_D1.8000.99
27_L80_V1.7590.99
49_L66_A1.7590.99
88_F111_V1.7450.99
67_A135_M1.7310.99
128_H132_E1.7260.99
62_D65_N1.6630.99
39_L171_L1.6600.99
90_S108_H1.6500.99
35_L168_A1.6260.98
49_L70_W1.6150.98
37_T40_T1.6000.98
128_H131_R1.5760.98
158_S162_S1.5730.98
165_R169_N1.5570.98
12_G25_K1.5500.98
22_L155_K1.5400.98
198_N210_G1.5270.98
110_E169_N1.5030.97
29_L52_R1.4860.97
38_I165_R1.4510.97
103_Y138_L1.4410.96
120_R123_K1.4380.96
20_N151_A1.4360.96
30_Q34_A1.4330.96
11_G25_K1.4230.96
11_G55_D1.4220.96
218_K223_K1.4180.96
38_I168_A1.4150.96
186_Y192_A1.3800.95
7_D63_R1.3780.95
74_F103_Y1.3780.95
142_N167_H1.3550.95
5_F66_A1.3540.95
193_F196_K1.3350.95
185_L190_I1.3330.95
186_Y189_Q1.3250.94
34_A157_S1.3030.94
197_A214_I1.2960.94
104_Q122_K1.2920.94
187_K191_G1.2810.93
62_D69_N1.2730.93
96_V126_N1.2680.93
111_V140_T1.2410.92
193_F202_L1.2330.92
129_V139_L1.2250.92
14_D25_K1.2200.91
89_E120_R1.2110.91
199_A206_A1.1980.91
4_I46_V1.1880.90
12_G55_D1.1870.90
4_I39_L1.1810.90
127_F139_L1.1780.90
91_Y127_F1.1730.90
208_A212_D1.1700.90
101_T124_T1.1490.89
93_Y137_A1.1240.88
52_R55_D1.1170.87
83_G146_D1.1040.87
115_V143_G1.0910.86
86_T118_A1.0770.85
12_G52_R1.0750.85
85_G146_D1.0740.85
7_D129_V1.0660.85
106_T174_A1.0660.85
84_G146_D1.0660.85
29_L55_D1.0600.84
143_G150_D1.0590.84
11_G14_D1.0560.84
117_F143_G1.0560.84
207_E213_S1.0490.84
14_D57_Y1.0480.84
215_V219_D1.0480.84
25_K29_L1.0470.84
18_T23_Q1.0440.83
4_I74_F1.0360.83
209_K212_D1.0350.83
181_S184_G1.0340.83
146_D150_D1.0320.83
218_K222_Y1.0200.82
91_Y125_A1.0150.82
68_N135_M1.0120.82
215_V218_K1.0110.81
40_T48_L1.0110.81
40_T53_T0.9980.81
196_K200_D0.9960.80
206_A211_F0.9820.80
194_K197_A0.9730.79
5_F72_A0.9670.78
106_T170_G0.9660.78
109_S173_Q0.9650.78
25_K52_R0.9640.78
91_Y123_K0.9630.78
61_N65_N0.9620.78
153_K162_S0.9560.78
153_K159_F0.9420.77
156_T159_F0.9280.76
51_S66_A0.9130.74
105_S109_S0.9100.74
35_L167_H0.9020.73
3_K71_G0.9010.73
151_A155_K0.8950.73
106_T173_Q0.8920.73
110_E113_Q0.8900.72
195_V204_S0.8880.72
200_D204_S0.8800.72
186_Y190_I0.8780.71
205_N209_K0.8780.71
34_A38_I0.8770.71
9_G141_E0.8770.71
61_N128_H0.8740.71
81_N121_G0.8690.71
14_D52_R0.8660.70
97_G131_R0.8600.70
96_V125_A0.8570.70
152_N156_T0.8520.69
60_L128_H0.8510.69
216_L222_Y0.8480.69
43_Y46_V0.8370.68
14_D29_L0.8360.68
188_V192_A0.8340.67
186_Y199_A0.8290.67
35_L78_I0.8250.67
193_F211_F0.8210.66
189_Q202_L0.8170.66
175_F179_K0.8140.66
196_K213_S0.8100.65
197_A204_S0.8030.65
202_L205_N0.8030.65
79_H141_E0.8020.64
35_L164_A0.7980.64
62_D66_A0.7950.64
39_L168_A0.7910.63
35_L76_L0.7770.62
10_H141_E0.7740.62
71_G99_P0.7720.61
12_G29_L0.7660.61
194_K198_N0.7650.61
38_I161_Q0.7620.60
43_Y172_E0.7620.60
196_K204_S0.7600.60
8_P50_L0.7590.60
194_K204_S0.7590.60
33_L36_R0.7530.60
108_H170_G0.7520.59
208_A213_S0.7500.59
14_D55_D0.7480.59
58_V63_R0.7440.59
32_A50_L0.7430.58
34_A160_I0.7390.58
216_L223_K0.7250.57
203_A206_A0.7210.56
39_L46_V0.7210.56
211_F214_I0.7160.56
15_P18_T0.7150.56
17_A91_Y0.7150.56
86_T149_S0.7090.55
28_T127_F0.7080.55
215_V223_K0.7000.54
210_G214_I0.6960.54
100_T122_K0.6940.53
153_K156_T0.6940.53
206_A210_G0.6940.53
39_L76_L0.6900.53
8_P51_S0.6900.53
14_D145_I0.6890.53
203_A207_E0.6850.52
29_L33_L0.6850.52
96_V127_F0.6820.52
219_D223_K0.6810.52
195_V201_S0.6810.52
27_L31_I0.6770.52
205_N208_A0.6760.51
74_F174_A0.6720.51
122_K127_F0.6690.51
207_E211_F0.6650.50
36_R53_T0.6650.50
217_L222_Y0.6650.50
185_L192_A0.6630.50
6_I35_L0.6620.50
204_S208_A0.6610.50
15_P31_I0.6610.50
68_N134_A0.6600.50
185_L188_V0.6580.50
196_K199_A0.6550.49
199_A211_F0.6530.49
57_Y60_L0.6530.49
190_I206_A0.6500.49
195_V205_N0.6460.48
88_F108_H0.6450.48
90_S140_T0.6440.48
36_R48_L0.6440.48
219_D222_Y0.6430.48
31_I163_L0.6410.48
187_K190_I0.6410.48
28_T78_I0.6390.48
127_F132_E0.6370.47
140_T170_G0.6350.47
72_A135_M0.6340.47
80_V151_A0.6330.47
33_L53_T0.6320.47
71_G171_L0.6310.47
100_T124_T0.6300.47
66_A70_W0.6280.46
220_G223_K0.6280.46
207_E217_L0.6270.46
218_K221_L0.6260.46
68_N119_D0.6260.46
88_F121_G0.6180.45
6_I171_L0.6180.45
183_S186_Y0.6170.45
67_A75_F0.6140.45
201_S212_D0.6090.44
5_F70_W0.6070.44
122_K126_N0.6060.44
197_A212_D0.6040.44
93_Y126_N0.6030.44
31_I80_V0.6030.44
7_D52_R0.6030.44
92_I124_T0.6030.44
182_S185_L0.6020.44
193_F201_S0.6020.44
51_S174_A0.6010.44
32_A35_L0.6000.43
7_D51_S0.5990.43
92_I101_T0.5980.43
31_I78_I0.5940.43
207_E212_D0.5920.43
193_F204_S0.5900.42
10_H79_H0.5900.42
22_L27_L0.5850.42
9_G79_H0.5820.42
34_A164_A0.5810.41
11_G129_V0.5780.41
107_I140_T0.5770.41
21_G155_K0.5770.41
180_T184_G0.5760.41
51_S75_F0.5730.41
97_G101_T0.5730.41
209_K213_S0.5730.41
94_P124_T0.5720.41
14_D27_L0.5710.40
38_I169_N0.5690.40
59_S128_H0.5670.40
97_G127_F0.5670.40
193_F198_N0.5620.40
207_E210_G0.5600.39
108_H117_F0.5540.39
183_S187_K0.5500.38
216_L221_L0.5500.38
36_R51_S0.5490.38
201_S208_A0.5490.38
205_N210_G0.5480.38
208_A214_I0.5470.38
125_A130_L0.5470.38
130_L139_L0.5450.38
75_F126_N0.5440.38
37_T180_T0.5410.37
28_T32_A0.5390.37
197_A210_G0.5390.37
9_G16_G0.5370.37
19_G155_K0.5370.37
17_A146_D0.5360.37
33_L54_S0.5340.37
116_D121_G0.5320.37
217_L220_G0.5300.36
91_Y139_L0.5280.36
194_K201_S0.5280.36
217_L221_L0.5280.36
192_A202_L0.5270.36
195_V211_F0.5250.36
75_F135_M0.5250.36
35_L172_E0.5250.36
193_F206_A0.5250.36
93_Y125_A0.5240.36
207_E218_K0.5230.36
75_F137_A0.5230.36
105_S170_G0.5220.36
172_E177_L0.5170.35
206_A215_V0.5170.35
203_A213_S0.5170.35
11_G52_R0.5160.35
215_V222_Y0.5150.35
19_G22_L0.5140.35
43_Y168_A0.5130.35
147_T160_I0.5130.35
198_N208_A0.5110.34
187_K192_A0.5100.34
215_V221_L0.5100.34
97_G126_N0.5090.34
93_Y96_V0.5080.34
197_A211_F0.5070.34
181_S188_V0.5070.34
201_S204_S0.5060.34
198_N201_S0.5060.34
48_L121_G0.5040.34
7_D77_S0.5030.34
64_T133_S0.5000.33
3_K49_L0.5000.33
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1jwqA 1 0.6824 100 0.262 Contact Map
4rn7A 1 0.6941 100 0.269 Contact Map
1xovA 1 0.9804 100 0.271 Contact Map
4binA 1 0.6863 100 0.274 Contact Map
3ne8A 2 0.7176 100 0.279 Contact Map
4lq6A 1 0.6902 100 0.305 Contact Map
3czxA 2 0.6706 100 0.318 Contact Map
3qayA 1 0.6588 100 0.354 Contact Map
1x60A 1 0.3098 97.2 0.897 Contact Map
1utaA 1 0.2941 96.8 0.903 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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