FLHF - BACSU - GREMLIN Contacts

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FLHF - Flagellar biosynthesis protein FlhF

UniProt:	Q01960	Sequences: MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLEKKTPQKAAEPKQTLKTPVSSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILCDMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTKEEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVPVNQYIFTKIDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	366 (284)
Sequences:	6652 (3208)
Seq/√Len:	190.4

ECOLI - FTSY SRP54
BACSU - FLHF FTSY SRP54
HALSA - Q9HMN3 SRP54
SULSO - Q980G1 SRP54

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
227_A	234_L	6.332	1.00
131_N	333_A	3.557	1.00
208_A	253_Y	3.387	1.00
199_S	255_H	3.385	1.00
303_V	307_S	3.126	1.00
272_D	275_K	3.075	1.00
180_L	192_L	3.007	1.00
210_I	246_A	2.976	1.00
135_K	158_I	2.874	1.00
206_K	253_Y	2.865	1.00
156_Q	362_R	2.840	1.00
158_I	161_D	2.834	1.00
224_K	236_V	2.776	1.00
275_K	308_S	2.681	1.00
224_K	228_E	2.634	1.00
109_P	137_L	2.423	1.00
221_E	224_K	2.355	1.00
209_F	227_A	2.351	1.00
243_F	258_V	2.300	1.00
287_F	315_I	2.297	1.00
197_A	202_E	2.288	1.00
237_C	245_Q	2.197	1.00
356_S	359_G	2.185	1.00
198_I	203_K	2.180	1.00
345_G	351_D	2.159	1.00
304_K	307_S	2.159	1.00
200_M	231_Q	2.154	1.00
285_Q	340_G	2.122	1.00
303_V	334_E	2.096	1.00
230_L	348_V	2.082	1.00
306_F	337_I	2.075	1.00
192_L	257_F	2.052	1.00
278_I	310_P	1.966	1.00
139_F	158_I	1.957	1.00
191_T	315_I	1.892	1.00
210_I	256_V	1.883	1.00
265_F	297_E	1.881	1.00
212_T	243_F	1.851	1.00
233_P	253_Y	1.834	1.00
147_T	150_N	1.773	1.00
302_I	335_S	1.768	1.00
160_C	358_L	1.725	1.00
208_A	233_P	1.701	1.00
316_F	328_V	1.697	1.00
287_F	313_Q	1.693	1.00
268_P	272_D	1.672	1.00
285_Q	312_N	1.669	1.00
171_E	176_K	1.659	1.00
198_I	341_F	1.658	1.00
225_T	228_E	1.658	1.00
311_V	337_I	1.656	1.00
200_M	232_A	1.620	1.00
180_L	257_F	1.611	1.00
135_K	161_D	1.601	1.00
139_F	157_E	1.586	1.00
131_N	135_K	1.564	1.00
198_I	354_T	1.562	1.00
171_E	174_H	1.558	1.00
196_A	209_F	1.537	1.00
193_A	226_Y	1.524	1.00
272_D	276_E	1.516	1.00
177_Y	247_K	1.514	1.00
287_F	340_G	1.500	1.00
124_I	329_F	1.462	1.00
292_A	316_F	1.454	1.00
197_A	350_E	1.453	1.00
159_L	329_F	1.404	1.00
160_C	361_V	1.397	1.00
192_L	209_F	1.396	1.00
353_Q	359_G	1.378	1.00
197_A	348_V	1.376	1.00
159_L	361_V	1.375	1.00
209_F	223_L	1.374	1.00
224_K	234_L	1.370	1.00
86_R	91_Q	1.367	1.00
178_I	255_H	1.359	1.00
205_K	254_D	1.344	1.00
180_L	195_L	1.340	1.00
247_K	256_V	1.326	1.00
193_A	230_L	1.315	1.00
196_A	232_A	1.308	1.00
313_Q	340_G	1.302	1.00
120_Q	129_K	1.302	1.00
242_E	276_E	1.291	1.00
179_V	258_V	1.288	1.00
156_Q	160_C	1.271	1.00
205_K	255_H	1.260	1.00
176_K	284_I	1.259	1.00
319_I	342_M	1.252	1.00
238_Y	241_E	1.247	1.00
235_E	245_Q	1.245	1.00
306_F	311_V	1.235	1.00
246_A	256_V	1.232	1.00
191_T	343_T	1.232	1.00
209_F	257_F	1.230	1.00
92_I	96_Q	1.222	1.00
208_A	256_V	1.221	1.00
127_S	334_E	1.219	1.00
245_Q	248_E	1.219	1.00
129_K	133_L	1.216	1.00
226_Y	348_V	1.215	1.00
275_K	279_P	1.214	1.00
344_N	353_Q	1.212	1.00
237_C	241_E	1.209	1.00
347_N	350_E	1.208	1.00
177_Y	258_V	1.193	1.00
80_S	84_E	1.174	1.00
153_G	157_E	1.173	1.00
300_K	304_K	1.167	1.00
290_L	316_F	1.159	1.00
180_L	315_I	1.159	1.00
300_K	303_V	1.156	1.00
220_V	236_V	1.143	1.00
191_T	352_I	1.131	1.00
258_V	281_E	1.122	1.00
186_A	192_L	1.121	1.00
294_A	298_D	1.119	1.00
221_E	239_T	1.107	1.00
130_T	134_K	1.105	1.00
244_Q	248_E	1.096	1.00
207_I	253_Y	1.087	1.00
197_A	200_M	1.074	1.00
177_Y	256_V	1.069	1.00
295_K	298_D	1.069	1.00
341_F	354_T	1.068	1.00
172_P	176_K	1.066	1.00
139_F	161_D	1.059	1.00
197_A	230_L	1.050	1.00
161_D	165_S	1.037	1.00
90_P	93_P	1.037	1.00
178_I	257_F	1.034	1.00
265_F	300_K	1.029	1.00
173_I	176_K	1.017	1.00
193_A	348_V	1.016	1.00
239_T	242_E	1.015	1.00
181_F	286_S	1.013	1.00
278_I	286_S	0.983	0.99
167_D	356_S	0.981	0.99
86_R	90_P	0.980	0.99
95_Q	99_G	0.979	0.99
314_Y	331_I	0.979	0.99
211_T	220_V	0.978	0.99
319_I	360_F	0.975	0.99
88_Y	92_I	0.974	0.99
132_T	136_L	0.963	0.99
299_M	330_N	0.963	0.99
220_V	237_C	0.958	0.99
87_T	90_P	0.955	0.99
131_N	134_K	0.953	0.99
134_K	138_R	0.949	0.99
128_T	333_A	0.944	0.99
212_T	258_V	0.942	0.99
238_Y	242_E	0.931	0.99
142_E	145_G	0.930	0.99
210_I	258_V	0.929	0.99
306_F	310_P	0.927	0.99
228_E	231_Q	0.917	0.99
170_Q	173_I	0.915	0.99
131_N	162_M	0.915	0.99
357_P	361_V	0.915	0.99
186_A	294_A	0.911	0.99
199_S	257_F	0.911	0.99
134_K	137_L	0.910	0.99
210_I	243_F	0.910	0.99
164_P	357_P	0.906	0.99
114_K	117_K	0.900	0.99
156_Q	365_C	0.897	0.99
116_E	129_K	0.893	0.99
139_F	143_A	0.884	0.99
209_F	234_L	0.884	0.99
123_G	297_E	0.881	0.99
263_R	273_E	0.879	0.99
179_V	286_S	0.877	0.99
198_I	202_E	0.874	0.99
200_M	230_L	0.874	0.99
290_L	331_I	0.874	0.99
343_T	352_I	0.873	0.99
237_C	242_E	0.871	0.99
243_F	281_E	0.865	0.99
276_E	279_P	0.860	0.98
176_K	285_Q	0.860	0.98
170_Q	176_K	0.845	0.98
288_L	314_Y	0.842	0.98
167_D	354_T	0.840	0.98
180_L	191_T	0.838	0.98
358_L	362_R	0.837	0.98
208_A	251_S	0.836	0.98
85_E	88_Y	0.831	0.98
135_K	138_R	0.830	0.98
83_I	87_T	0.823	0.98
275_K	307_S	0.818	0.98
177_Y	284_I	0.814	0.98
207_I	233_P	0.812	0.98
119_L	124_I	0.810	0.98
286_S	309_V	0.809	0.98
328_V	339_V	0.809	0.98
109_P	113_R	0.806	0.98
159_L	365_C	0.805	0.98
93_P	97_E	0.803	0.98
227_A	230_L	0.801	0.98
269_Q	272_D	0.798	0.98
154_K	158_I	0.797	0.98
299_M	334_E	0.797	0.98
130_T	133_L	0.794	0.97
100_D	107_V	0.791	0.97
96_Q	100_D	0.788	0.97
81_P	84_E	0.783	0.97
179_V	277_T	0.782	0.97
107_V	151_V	0.782	0.97
304_K	308_S	0.781	0.97
81_P	85_E	0.781	0.97
118_L	121_E	0.779	0.97
138_R	142_E	0.777	0.97
170_Q	174_H	0.775	0.97
139_F	154_K	0.772	0.97
166_A	169_W	0.771	0.97
135_K	333_A	0.770	0.97
226_Y	230_L	0.768	0.97
224_K	227_A	0.767	0.97
265_F	301_H	0.765	0.97
160_C	357_P	0.763	0.97
94_A	97_E	0.762	0.97
117_K	121_E	0.762	0.97
164_P	358_L	0.760	0.97
136_L	158_I	0.759	0.97
160_C	362_R	0.759	0.97
298_D	322_T	0.754	0.97
112_L	137_L	0.754	0.97
285_Q	313_Q	0.752	0.96
344_N	352_I	0.751	0.96
124_I	132_T	0.749	0.96
210_I	235_E	0.749	0.96
235_E	249_L	0.746	0.96
158_I	162_M	0.744	0.96
209_F	230_L	0.741	0.96
96_Q	110_E	0.741	0.96
197_A	352_I	0.740	0.96
226_Y	229_L	0.738	0.96
306_F	309_V	0.734	0.96
249_L	252_E	0.730	0.96
332_L	336_K	0.721	0.96
184_T	346_Q	0.720	0.96
290_L	298_D	0.719	0.95
278_I	309_V	0.717	0.95
292_A	319_I	0.715	0.95
220_V	224_K	0.713	0.95
286_S	312_N	0.712	0.95
243_F	247_K	0.712	0.95
112_L	151_V	0.710	0.95
125_K	330_N	0.706	0.95
112_L	133_L	0.706	0.95
95_Q	98_L	0.706	0.95
160_C	164_P	0.704	0.95
199_S	203_K	0.703	0.95
218_A	261_A	0.703	0.95
146_L	150_N	0.702	0.95
227_A	233_P	0.701	0.95
211_T	237_C	0.701	0.95
233_P	251_S	0.700	0.95
272_D	279_P	0.697	0.95
179_V	278_I	0.695	0.95
277_T	281_E	0.693	0.94
183_S	266_K	0.693	0.94
111_P	148_E	0.692	0.94
299_M	335_S	0.692	0.94
157_E	161_D	0.691	0.94
170_Q	255_H	0.688	0.94
321_E	346_Q	0.688	0.94
107_V	110_E	0.686	0.94
108_L	111_P	0.686	0.94
112_L	140_S	0.683	0.94
180_L	287_F	0.681	0.94
265_F	304_K	0.681	0.94
181_F	232_A	0.681	0.94
157_E	160_C	0.676	0.94
139_F	144_G	0.675	0.94
143_A	150_N	0.672	0.94
213_D	222_Q	0.672	0.94
95_Q	100_D	0.672	0.94
220_V	239_T	0.669	0.93
244_Q	280_F	0.667	0.93
218_A	265_F	0.664	0.93
206_K	252_E	0.662	0.93
133_L	137_L	0.660	0.93
276_E	280_F	0.658	0.93
297_E	300_K	0.657	0.93
174_H	249_L	0.657	0.93
333_A	336_K	0.654	0.93
140_S	154_K	0.653	0.93
232_A	240_K	0.652	0.92
211_T	236_V	0.652	0.92
165_S	357_P	0.651	0.92
149_E	152_V	0.651	0.92
257_F	352_I	0.646	0.92
298_D	327_S	0.645	0.92
127_S	130_T	0.642	0.92
140_S	151_V	0.642	0.92
217_I	226_Y	0.641	0.92
235_E	251_S	0.640	0.92
103_A	142_E	0.638	0.92
199_S	204_H	0.637	0.92
116_E	133_L	0.634	0.91
216_R	222_Q	0.633	0.91
243_F	277_T	0.630	0.91
97_E	102_S	0.630	0.91
286_S	311_V	0.628	0.91
310_P	337_I	0.628	0.91
206_K	254_D	0.627	0.91
230_L	233_P	0.627	0.91
295_K	322_T	0.627	0.91
152_V	156_Q	0.626	0.91
113_R	117_K	0.625	0.91
237_C	251_S	0.622	0.91
212_T	277_T	0.621	0.91
270_Y	274_L	0.618	0.90
227_A	236_V	0.614	0.90
206_K	233_P	0.614	0.90
97_E	101_F	0.613	0.90
207_I	257_F	0.612	0.90
195_L	199_S	0.612	0.90
196_A	230_L	0.611	0.90
299_M	327_S	0.611	0.90
108_L	112_L	0.610	0.90
166_A	332_L	0.608	0.90
183_S	186_A	0.607	0.90
202_E	350_E	0.605	0.89
83_I	363_M	0.605	0.89
263_R	271_I	0.602	0.89
209_F	232_A	0.602	0.89
315_I	341_F	0.600	0.89
86_R	344_N	0.597	0.89
211_T	234_L	0.596	0.89
208_A	250_F	0.594	0.89
88_Y	91_Q	0.594	0.89
132_T	158_I	0.593	0.88
87_T	91_Q	0.591	0.88
181_F	309_V	0.591	0.88
83_I	344_N	0.588	0.88
106_S	109_P	0.587	0.88
243_F	246_A	0.587	0.88
303_V	331_I	0.586	0.88
184_T	215_Y	0.586	0.88
267_D	270_Y	0.584	0.88
179_V	329_F	0.578	0.87
97_E	118_L	0.576	0.87
342_M	355_V	0.576	0.87
192_L	294_A	0.573	0.87
286_S	310_P	0.573	0.87
184_T	264_N	0.572	0.87
275_K	309_V	0.571	0.87
236_V	239_T	0.570	0.86
226_Y	346_Q	0.570	0.86
192_L	212_T	0.569	0.86
220_V	238_Y	0.569	0.86
123_G	295_K	0.569	0.86
207_I	254_D	0.568	0.86
196_A	348_V	0.566	0.86
127_S	333_A	0.564	0.86
199_S	205_K	0.563	0.86
237_C	240_K	0.562	0.86
355_V	360_F	0.562	0.86
181_F	311_V	0.561	0.86
99_G	104_Y	0.561	0.86
297_E	301_H	0.560	0.86
328_V	363_M	0.560	0.86
267_D	307_S	0.560	0.86
245_Q	251_S	0.559	0.85
281_E	284_I	0.559	0.85
118_L	152_V	0.558	0.85
317_T	321_E	0.557	0.85
229_L	351_D	0.556	0.85
171_E	175_S	0.553	0.85
88_Y	93_P	0.551	0.85
327_S	330_N	0.550	0.85
83_I	90_P	0.547	0.84
140_S	148_E	0.546	0.84
182_G	185_G	0.545	0.84
300_K	334_E	0.545	0.84
186_A	267_D	0.545	0.84
258_V	284_I	0.544	0.84
220_V	242_E	0.542	0.84
107_V	112_L	0.541	0.84
83_I	86_R	0.541	0.84
103_A	141_V	0.538	0.83
197_A	229_L	0.538	0.83
186_A	292_A	0.538	0.83
178_I	341_F	0.537	0.83
175_S	256_V	0.536	0.83
175_S	254_D	0.535	0.83
219_A	222_Q	0.535	0.83
200_M	206_K	0.535	0.83
187_G	259_D	0.531	0.82
89_P	92_I	0.530	0.82
348_V	351_D	0.529	0.82
143_A	154_K	0.529	0.82
265_F	271_I	0.529	0.82
332_L	340_G	0.528	0.82
130_T	135_K	0.526	0.82
129_K	132_T	0.526	0.82
108_L	141_V	0.525	0.82
298_D	302_I	0.524	0.82
295_K	324_S	0.524	0.82
143_A	147_T	0.520	0.81
247_K	280_F	0.518	0.81
82_K	86_R	0.516	0.81
164_P	167_D	0.516	0.81
295_K	325_L	0.515	0.80
86_R	93_P	0.514	0.80
243_F	256_V	0.513	0.80
103_A	112_L	0.513	0.80
268_P	308_S	0.510	0.80
220_V	318_K	0.510	0.80
112_L	136_L	0.509	0.80
120_Q	126_E	0.509	0.80
84_E	88_Y	0.509	0.80
183_S	321_E	0.508	0.80
222_Q	262_G	0.507	0.79
313_Q	338_G	0.507	0.79
163_L	360_F	0.507	0.79
80_S	83_I	0.506	0.79
119_L	129_K	0.505	0.79
141_V	144_G	0.503	0.79
261_A	289_V	0.501	0.79
124_I	326_G	0.501	0.79
221_E	238_Y	0.500	0.79
101_F	118_L	0.500	0.79

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2px0A	4	0.7049	100	0.487	Contact Map
1vmaA	1	0.7678	100	0.531	Contact Map
2yhsA	1	0.8005	100	0.546	Contact Map
2og2A	1	0.7814	100	0.553	Contact Map
2v3cC	1	0.7705	100	0.563	Contact Map
4c7oA	1	0.7732	100	0.563	Contact Map
3b9qA	1	0.7623	100	0.566	Contact Map
1ls1A	1	0.7568	100	0.577	Contact Map
2j37W	1	0.7514	100	0.588	Contact Map
1zu4A	1	0.7678	100	0.589	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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