GREMLIN Database
MISCA - Membrane protein insertase MisCA
UniProt: Q01625 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 261 (209)
Sequences: 4513 (2938)
Seq/√Len: 203.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
181_G214_I3.9251.00
121_A125_K3.7601.00
98_Q101_K3.7411.00
118_E121_A3.7101.00
96_E99_K3.5371.00
94_Q98_Q2.9331.00
183_A232_N2.7911.00
48_E52_Y2.7521.00
100_L115_L2.7021.00
98_Q102_E2.6751.00
113_Q117_Q2.4401.00
164_W171_D2.4391.00
96_E125_K2.4101.00
114_K118_E2.3771.00
41_Y45_P2.3341.00
114_K117_Q2.3001.00
66_I230_V2.2721.00
96_E126_H2.2361.00
148_Y152_M2.1801.00
141_M145_I2.1611.00
57_T67_L2.1421.00
115_L119_T2.0941.00
74_L234_F2.0171.00
179_V229_V2.0021.00
97_M119_T1.9841.00
93_L128_V1.9041.00
93_L122_L1.8931.00
166_D169_E1.8761.00
77_L81_I1.8241.00
212_M216_F1.8081.00
101_K105_S1.7931.00
101_K115_L1.7231.00
145_I149_H1.6901.00
189_K207_W1.6431.00
117_Q121_A1.6411.00
65_I223_A1.6071.00
100_L122_L1.5671.00
174_Y177_P1.5621.00
187_Q236_I1.5581.00
81_I85_R1.5151.00
103_K118_E1.5051.00
89_A92_A1.4901.00
119_T123_F1.4731.00
203_A207_W1.4601.00
138_L142_P1.4471.00
174_Y218_I1.4351.00
237_A241_L1.4321.00
185_F189_K1.4321.00
116_Q120_M1.4081.00
99_K103_K1.3941.00
53_V67_L1.3911.00
149_H152_M1.3781.00
204_M208_I1.3671.00
54_A61_Y1.3631.00
99_K102_E1.3571.00
88_K91_Q1.3531.00
116_Q119_T1.3531.00
63_L66_I1.3491.00
149_H153_R1.3361.00
241_L245_P1.3141.00
63_L67_L1.2981.00
52_Y56_L1.2711.00
51_T55_K1.2711.00
185_F207_W1.2661.00
162_F165_F1.2591.00
73_R235_M1.2551.00
102_E105_S1.2511.00
204_M207_W1.2501.00
182_V186_V1.2331.00
97_M122_L1.2281.00
74_L77_L1.2191.00
82_K85_R1.2031.00
112_Q116_Q1.1971.00
83_Q87_S1.1971.00
215_V219_N1.1891.00
189_K193_A1.1801.00
171_D177_P1.1801.00
76_I143_I1.1651.00
178_I182_V1.1511.00
200_P203_A1.1431.00
137_I141_M1.1401.00
214_I218_I1.1381.00
217_A225_S1.1201.00
209_M212_M1.1151.00
105_S115_L1.1101.00
85_R89_A1.1051.00
62_G162_F1.0991.00
201_Q204_M1.0991.00
53_V71_L1.0971.00
187_Q235_M1.0921.00
54_A64_S1.0831.00
71_L75_L1.0691.00
240_F244_G1.0681.00
104_Y107_K1.0681.00
73_R238_Q1.0561.00
200_P204_M1.0491.00
107_K114_K1.0371.00
233_L237_A1.0361.00
164_W222_A1.0191.00
155_Q158_S1.0171.00
237_A240_F1.0091.00
179_V183_A1.0041.00
121_A124_Q1.0021.00
104_Y114_K0.9991.00
171_D174_Y0.9981.00
47_S154_T0.9881.00
50_I64_S0.9851.00
164_W172_P0.9781.00
176_L222_A0.9620.99
183_A229_V0.9610.99
52_Y55_K0.9600.99
69_T231_G0.9590.99
211_I215_V0.9560.99
190_L240_F0.9550.99
89_A126_H0.9540.99
66_I226_L0.9520.99
231_G235_M0.9490.99
105_S112_Q0.9470.99
154_T157_I0.9450.99
232_N235_M0.9410.99
161_S164_W0.9300.99
201_Q205_M0.9260.99
208_I212_M0.9220.99
120_M124_Q0.9160.99
170_K174_Y0.9140.99
93_L126_H0.9140.99
188_Q191_M0.9120.99
228_W232_N0.9030.99
53_V68_V0.9000.99
44_Y48_E0.8930.99
92_A126_H0.8840.99
62_G226_L0.8800.99
39_D45_P0.8720.99
38_W41_Y0.8670.99
176_L225_S0.8550.99
49_L52_Y0.8470.99
220_F228_W0.8420.99
123_F130_P0.8410.99
183_A187_Q0.8380.99
53_V64_S0.8370.99
68_V147_F0.8320.98
53_V57_T0.8290.98
70_I234_F0.8270.98
171_D222_A0.8250.98
76_I140_Q0.8130.98
70_I230_V0.8100.98
40_K44_Y0.8100.98
180_A229_V0.8100.98
172_P175_I0.8090.98
184_T232_N0.8070.98
49_L68_V0.8060.98
161_S166_D0.8050.98
155_Q159_E0.7980.98
199_N202_M0.7960.98
81_I242_I0.7900.98
73_R80_M0.7850.98
73_R76_I0.7830.98
48_E51_T0.7820.98
183_A233_L0.7820.98
217_A228_W0.7720.98
78_P82_K0.7720.98
47_S51_T0.7720.98
82_K86_S0.7700.98
176_L226_L0.7670.97
45_P49_L0.7630.97
77_L241_L0.7590.97
141_M144_L0.7570.97
216_F220_F0.7560.97
73_R231_G0.7560.97
214_I217_A0.7540.97
220_F224_L0.7540.97
37_F41_Y0.7520.97
93_L123_F0.7500.97
186_V189_K0.7490.97
112_Q115_L0.7480.97
88_K92_A0.7470.97
245_P248_K0.7410.97
87_S90_M0.7360.97
191_M194_G0.7360.97
107_K113_Q0.7360.97
62_G66_I0.7320.97
39_D42_V0.7300.97
107_K112_Q0.7270.96
143_I147_F0.7190.96
67_L71_L0.7180.96
96_E122_L0.7170.96
148_Y220_F0.7130.96
92_A250_N0.7110.96
84_L242_I0.7080.96
226_L230_V0.7060.96
122_L126_H0.7050.96
40_K43_V0.7000.96
68_V72_I0.6960.95
158_S166_D0.6950.95
142_P146_G0.6940.95
113_Q116_Q0.6930.95
53_V56_L0.6930.95
84_L87_S0.6910.95
73_R140_Q0.6910.95
118_E125_K0.6890.95
198_Q201_Q0.6890.95
176_L229_V0.6830.95
129_N134_C0.6820.95
193_A196_A0.6820.95
144_L235_M0.6800.95
139_I142_P0.6800.95
49_L71_L0.6790.95
115_L118_E0.6760.95
83_Q143_I0.6750.95
100_L105_S0.6750.95
38_W45_P0.6730.95
196_A199_N0.6720.95
208_I211_I0.6710.95
66_I162_F0.6710.95
47_S150_A0.6650.94
170_K219_N0.6570.94
165_F176_L0.6560.94
245_P249_K0.6480.93
43_V153_R0.6470.93
144_L231_G0.6430.93
183_A236_I0.6370.93
161_S165_F0.6370.93
86_S133_G0.6360.93
39_D43_V0.6330.93
180_A213_I0.6330.93
212_M215_V0.6300.92
63_L162_F0.6280.92
172_P177_P0.6230.92
80_M242_I0.6230.92
137_I140_Q0.6210.92
181_G185_F0.6200.92
128_V132_A0.6180.92
117_Q120_M0.6150.92
65_I151_I0.6140.92
106_S115_L0.6130.91
197_Q203_A0.6120.91
101_K123_F0.6100.91
81_I84_L0.6100.91
158_S168_G0.6070.91
104_Y115_L0.6040.91
45_P48_E0.6040.91
90_M94_Q0.6020.91
180_A214_I0.5990.90
178_I181_G0.5980.90
192_M206_L0.5970.90
156_A160_H0.5950.90
178_I214_I0.5910.90
106_S112_Q0.5900.90
46_L150_A0.5830.89
54_A60_N0.5820.89
186_V190_L0.5790.89
211_I214_I0.5760.89
173_Y177_P0.5750.89
72_I147_F0.5750.89
57_T63_L0.5730.88
185_F206_L0.5720.88
229_V233_L0.5720.88
84_L88_K0.5690.88
41_Y44_Y0.5690.88
42_V45_P0.5690.88
188_Q192_M0.5680.88
100_L119_T0.5660.88
164_W177_P0.5660.88
42_V146_G0.5650.88
40_K216_F0.5620.88
177_P214_I0.5570.87
241_L244_G0.5550.87
43_V149_H0.5550.87
88_K243_K0.5550.87
164_W170_K0.5530.87
80_M83_Q0.5530.87
157_I168_G0.5520.87
166_D171_D0.5510.86
76_I83_Q0.5490.86
155_Q161_S0.5430.86
165_F222_A0.5430.86
199_N203_A0.5410.85
148_Y228_W0.5390.85
86_S90_M0.5380.85
100_L104_Y0.5380.85
55_K170_K0.5370.85
80_M84_L0.5360.85
72_I227_Y0.5340.85
184_T228_W0.5270.84
87_S191_M0.5230.84
83_Q137_I0.5220.83
45_P148_Y0.5220.83
135_F138_L0.5220.83
183_A186_V0.5210.83
101_K119_T0.5210.83
213_I217_A0.5210.83
97_M123_F0.5200.83
81_I241_L0.5190.83
164_W174_Y0.5190.83
220_F225_S0.5170.83
93_L97_M0.5130.82
187_Q190_L0.5120.82
202_M205_M0.5120.82
158_S169_E0.5120.82
89_A127_G0.5100.82
180_A225_S0.5100.82
83_Q132_A0.5090.82
86_S132_A0.5090.82
82_K132_A0.5080.82
37_F42_V0.5080.82
205_M216_F0.5070.82
136_P210_P0.5060.82
187_Q232_N0.5060.82
43_V145_I0.5040.81
167_L226_L0.5040.81
206_L210_P0.5040.81
170_K173_Y0.5020.81
113_Q120_M0.5020.81
71_L74_L0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3wo6A 1 0.7969 100 0.235 Contact Map
3wvfA 1 0.8429 100 0.241 Contact Map
3vouA 4 0.4176 18.6 0.956 Contact Map
3effK 4 0.4559 16.1 0.957 Contact Map
4h33A 3 0.2835 12.8 0.959 Contact Map
3pjsK 4 0.4521 9.2 0.962 Contact Map
3rvyA 3 0.3257 8 0.962 Contact Map
4ja0A 6 0.4023 5.6 0.965 Contact Map
3blcA 4 0.1992 4.8 0.966 Contact Map
2q2fA 2 0.2184 4.2 0.967 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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