MREB - BACSU - GREMLIN Contacts

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MREB - Rod shape-determining protein MreB

UniProt:	Q01465	Sequences: MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQTDTKSIVAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPYVMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIGGGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSAEAPEESDNMEIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELAADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKGKTR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	337 (313)
Sequences:	11088 (6303)
Seq/√Len:	356.3

ECOLI - MREB HSCC DNAK HSCA YEGD FTSA EUTJ
BACSU - MREB MBL MREBH DNAK FTSA
HALSA - DNAK

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
102_Y	129_R	3.469	1.00
157_V	260_I	3.259	1.00
179_S	259_T	3.152	1.00
216_G	220_A	3.041	1.00
190_A	255_D	3.006	1.00
226_N	243_E	2.963	1.00
265_K	303_E	2.947	1.00
198_T	248_E	2.865	1.00
63_V	185_D	2.717	1.00
313_D	316_D	2.614	1.00
18_T	86_Y	2.533	1.00
105_V	123_T	2.353	1.00
254_R	258_S	2.349	1.00
298_K	302_E	2.295	1.00
168_I	267_T	2.257	1.00
80_A	125_Q	2.226	1.00
87_I	91_I	2.225	1.00
301_S	307_P	2.201	1.00
191_I	244_I	2.195	1.00
210_A	214_E	2.189	1.00
290_G	293_L	2.141	1.00
106_C	322_T	2.128	1.00
117_R	121_D	2.079	1.00
24_G	27_I	2.076	1.00
245_T	248_E	2.040	1.00
261_V	303_E	2.038	1.00
37_L	81_T	2.012	1.00
85_Y	89_Q	1.971	1.00
299_V	302_E	1.964	1.00
248_E	251_N	1.955	1.00
153_G	170_S	1.921	1.00
292_L	315_L	1.896	1.00
21_F	323_G	1.881	1.00
187_M	256_T	1.880	1.00
297_D	310_I	1.867	1.00
194_Y	252_A	1.835	1.00
159_I	164_T	1.806	1.00
261_V	299_V	1.799	1.00
16_A	161_G	1.791	1.00
194_Y	198_T	1.783	1.00
168_I	271_T	1.732	1.00
257_V	295_N	1.717	1.00
253_L	293_L	1.717	1.00
228_E	241_T	1.710	1.00
15_T	162_G	1.703	1.00
228_E	243_E	1.691	1.00
18_T	33_S	1.690	1.00
249_I	293_L	1.684	1.00
120_I	124_R	1.645	1.00
187_M	253_L	1.642	1.00
73_I	78_T	1.639	1.00
81_T	84_K	1.632	1.00
152_T	281_D	1.632	1.00
49_N	53_N	1.627	1.00
190_A	193_N	1.624	1.00
20_V	86_Y	1.622	1.00
21_F	319_A	1.614	1.00
316_D	320_I	1.611	1.00
102_Y	131_A	1.601	1.00
195_I	215_I	1.590	1.00
246_G	250_S	1.587	1.00
155_M	168_I	1.582	1.00
195_I	242_I	1.581	1.00
76_Y	121_D	1.576	1.00
87_I	128_A	1.572	1.00
251_N	254_R	1.557	1.00
192_I	212_K	1.550	1.00
121_D	124_R	1.546	1.00
254_R	299_V	1.543	1.00
218_A	229_I	1.535	1.00
253_L	257_V	1.525	1.00
257_V	299_V	1.501	1.00
112_T	115_E	1.481	1.00
266_S	270_K	1.477	1.00
84_K	88_N	1.471	1.00
9_L	20_V	1.461	1.00
79_T	119_V	1.458	1.00
19_L	30_R	1.457	1.00
159_I	187_M	1.451	1.00
262_E	266_S	1.432	1.00
261_V	265_K	1.416	1.00
58_T	61_N	1.415	1.00
253_L	256_T	1.412	1.00
267_T	271_T	1.412	1.00
190_A	256_T	1.404	1.00
250_S	298_K	1.382	1.00
107_V	133_P	1.382	1.00
67_P	70_D	1.381	1.00
264_V	300_I	1.380	1.00
77_E	125_Q	1.373	1.00
274_E	277_A	1.334	1.00
85_Y	88_N	1.332	1.00
168_I	279_I	1.325	1.00
313_D	320_I	1.323	1.00
214_E	217_S	1.318	1.00
19_L	319_A	1.317	1.00
135_E	138_F	1.308	1.00
197_K	200_N	1.306	1.00
45_V	81_T	1.302	1.00
198_T	201_L	1.301	1.00
286_L	300_I	1.289	1.00
166_V	267_T	1.289	1.00
164_T	260_I	1.288	1.00
157_V	164_T	1.281	1.00
155_M	166_V	1.281	1.00
194_Y	248_E	1.280	1.00
116_E	135_E	1.278	1.00
178_Q	266_S	1.272	1.00
152_T	280_M	1.270	1.00
217_S	221_P	1.258	1.00
295_N	298_K	1.258	1.00
64_A	79_T	1.256	1.00
189_D	193_N	1.248	1.00
191_I	215_I	1.247	1.00
13_L	79_T	1.246	1.00
10_G	322_T	1.245	1.00
23_K	327_E	1.234	1.00
87_I	103_V	1.228	1.00
102_Y	130_D	1.209	1.00
262_E	265_K	1.203	1.00
298_K	301_S	1.203	1.00
299_V	303_E	1.200	1.00
35_V	78_T	1.198	1.00
17_N	315_L	1.198	1.00
8_D	326_L	1.198	1.00
254_R	295_N	1.190	1.00
91_I	99_R	1.189	1.00
109_S	137_P	1.189	1.00
155_M	264_V	1.188	1.00
195_I	229_I	1.186	1.00
297_D	301_S	1.180	1.00
65_L	136_E	1.170	1.00
88_N	92_K	1.170	1.00
8_D	131_A	1.169	1.00
187_M	290_G	1.168	1.00
258_S	262_E	1.163	1.00
218_A	227_M	1.159	1.00
300_I	308_V	1.158	1.00
13_L	119_V	1.153	1.00
284_I	304_T	1.151	1.00
134_I	325_A	1.143	1.00
268_L	276_A	1.142	1.00
136_E	165_E	1.141	1.00
190_A	253_L	1.128	1.00
64_A	78_T	1.122	1.00
187_M	293_L	1.121	1.00
153_G	273_P	1.119	1.00
166_V	263_A	1.112	1.00
31_E	89_Q	1.103	1.00
159_I	296_L	1.100	1.00
187_M	249_I	1.093	1.00
286_L	291_A	1.082	1.00
64_A	82_M	1.070	1.00
189_D	212_K	1.066	1.00
230_R	241_T	1.060	1.00
192_I	215_I	1.052	1.00
124_R	129_R	1.052	1.00
10_G	319_A	1.048	1.00
295_N	299_V	1.048	1.00
221_P	224_S	1.046	1.00
113_A	117_R	1.044	1.00
162_G	165_E	1.042	1.00
47_V	85_Y	1.041	1.00
325_A	328_H	1.037	1.00
218_A	244_I	1.036	1.00
203_I	238_L	1.030	1.00
18_T	82_M	1.028	1.00
38_Q	41_T	1.028	1.00
274_E	278_D	1.024	1.00
36_A	62_V	1.019	1.00
134_I	139_A	1.017	1.00
281_D	309_L	1.014	1.00
36_A	54_M	1.011	1.00
249_I	290_G	1.010	1.00
158_D	165_E	1.003	1.00
184_G	290_G	1.002	1.00
189_D	192_I	0.996	1.00
86_Y	89_Q	0.994	1.00
8_D	102_Y	0.990	1.00
266_S	269_E	0.978	1.00
198_T	202_M	0.978	1.00
36_A	46_A	0.966	1.00
194_Y	244_I	0.952	1.00
140_A	318_V	0.952	1.00
168_I	273_P	0.950	1.00
88_N	91_I	0.950	1.00
73_I	81_T	0.948	1.00
89_Q	92_K	0.942	1.00
120_I	133_P	0.933	1.00
140_A	287_T	0.930	1.00
158_D	163_T	0.929	1.00
58_T	62_V	0.924	1.00
37_L	73_I	0.924	1.00
208_A	212_K	0.922	1.00
13_L	83_M	0.920	1.00
258_S	261_V	0.919	1.00
225_D	246_G	0.917	1.00
247_K	250_S	0.915	1.00
287_T	317_C	0.915	1.00
144_A	285_V	0.913	1.00
161_G	165_E	0.911	1.00
33_S	82_M	0.908	1.00
250_S	295_N	0.904	1.00
121_D	125_Q	0.900	1.00
198_T	242_I	0.899	1.00
76_Y	118_A	0.899	1.00
233_D	240_K	0.894	1.00
101_P	129_R	0.892	1.00
221_P	227_M	0.889	1.00
158_D	162_G	0.884	1.00
265_K	269_E	0.882	1.00
80_A	84_K	0.876	1.00
248_E	252_A	0.875	1.00
258_S	299_V	0.873	1.00
188_D	216_G	0.871	1.00
107_V	135_E	0.868	1.00
75_D	79_T	0.867	1.00
9_L	90_A	0.864	1.00
320_I	324_K	0.861	1.00
54_M	58_T	0.857	1.00
182_V	259_T	0.854	1.00
250_S	254_R	0.853	1.00
263_A	267_T	0.850	1.00
104_M	326_L	0.849	1.00
117_R	120_I	0.848	1.00
105_V	119_V	0.846	1.00
107_V	116_E	0.845	1.00
34_V	52_K	0.845	1.00
158_D	161_G	0.844	1.00
159_I	290_G	0.841	1.00
253_L	295_N	0.836	1.00
116_E	120_I	0.834	1.00
259_T	262_E	0.833	1.00
301_S	308_V	0.832	1.00
214_E	218_A	0.830	1.00
80_A	122_A	0.827	1.00
66_R	186_E	0.824	1.00
187_M	257_V	0.823	1.00
226_N	245_T	0.823	1.00
9_L	103_V	0.821	1.00
37_L	78_T	0.821	1.00
193_N	197_K	0.816	1.00
11_I	83_M	0.814	1.00
143_G	317_C	0.811	1.00
179_S	182_V	0.808	1.00
216_G	219_E	0.796	1.00
113_A	116_E	0.795	1.00
60_G	205_D	0.793	1.00
261_V	264_V	0.793	1.00
132_Y	325_A	0.793	1.00
159_I	260_I	0.791	1.00
141_A	169_I	0.776	1.00
194_Y	242_I	0.774	1.00
17_N	30_R	0.772	1.00
249_I	253_L	0.771	1.00
13_L	64_A	0.770	1.00
137_P	165_E	0.769	1.00
264_V	303_E	0.767	1.00
153_G	280_M	0.767	1.00
153_G	279_I	0.765	1.00
9_L	100_K	0.758	1.00
247_K	251_N	0.758	1.00
155_M	279_I	0.757	1.00
47_V	82_M	0.757	1.00
273_P	278_D	0.753	1.00
46_A	50_D	0.753	1.00
74_A	77_E	0.753	1.00
215_I	220_A	0.751	1.00
123_T	133_P	0.746	1.00
65_L	163_T	0.744	1.00
123_T	126_A	0.742	1.00
227_M	232_R	0.741	1.00
77_E	81_T	0.740	1.00
192_I	196_R	0.735	1.00
182_V	186_E	0.733	1.00
91_I	100_K	0.733	1.00
29_V	93_N	0.730	1.00
214_E	227_M	0.729	1.00
160_G	289_G	0.728	1.00
103_V	128_A	0.726	0.99
77_E	80_A	0.726	0.99
143_G	320_I	0.722	0.99
142_I	325_A	0.721	0.99
177_S	263_A	0.720	0.99
244_I	252_A	0.719	0.99
137_P	140_A	0.719	0.99
10_G	323_G	0.719	0.99
222_E	294_R	0.718	0.99
79_T	83_M	0.717	0.99
111_I	119_V	0.715	0.99
200_N	203_I	0.714	0.99
177_S	266_S	0.710	0.99
116_E	133_P	0.709	0.99
68_M	79_T	0.704	0.99
309_L	317_C	0.702	0.99
144_A	317_C	0.699	0.99
76_Y	80_A	0.699	0.99
137_P	163_T	0.698	0.99
81_T	85_Y	0.697	0.99
36_A	58_T	0.696	0.99
111_I	115_E	0.695	0.99
244_I	249_I	0.693	0.99
286_L	310_I	0.685	0.99
181_R	186_E	0.685	0.99
68_M	119_V	0.683	0.99
136_E	163_T	0.681	0.99
185_D	188_D	0.678	0.99
157_V	264_V	0.677	0.99
157_V	166_V	0.677	0.99
12_D	15_T	0.677	0.99
138_F	169_I	0.676	0.99
192_I	208_A	0.676	0.99
30_R	316_D	0.675	0.99
145_N	148_V	0.675	0.99
49_N	52_K	0.671	0.99
210_A	232_R	0.668	0.99
15_T	163_T	0.663	0.99
194_Y	197_K	0.662	0.99
215_I	218_A	0.661	0.99
246_G	294_R	0.655	0.99
136_E	318_V	0.655	0.99
136_E	161_G	0.654	0.99
107_V	119_V	0.654	0.99
193_N	196_R	0.646	0.99
161_G	289_G	0.646	0.99
199_Y	203_I	0.646	0.99
166_V	177_S	0.644	0.99
217_S	220_A	0.642	0.99
76_Y	125_Q	0.641	0.99
232_R	237_G	0.641	0.99
109_S	119_V	0.639	0.99
276_A	303_E	0.638	0.99
54_M	57_R	0.638	0.99
11_I	103_V	0.636	0.99
140_A	161_G	0.636	0.99
122_A	126_A	0.636	0.99
224_S	246_G	0.636	0.99
123_T	128_A	0.632	0.99
22_V	93_N	0.631	0.99
198_T	240_K	0.630	0.99
191_I	252_A	0.628	0.99
50_D	53_N	0.627	0.99
106_C	318_V	0.625	0.99
210_A	239_P	0.625	0.99
37_L	45_V	0.622	0.98
160_G	163_T	0.621	0.98
276_A	304_T	0.620	0.98
19_L	28_V	0.619	0.98
177_S	267_T	0.619	0.98
84_K	87_I	0.618	0.98
64_A	68_M	0.618	0.98
229_I	232_R	0.618	0.98
31_E	85_Y	0.618	0.98
153_G	278_D	0.611	0.98
258_S	303_E	0.610	0.98
83_M	87_I	0.610	0.98
257_V	261_V	0.607	0.98
12_D	158_D	0.604	0.98
46_A	54_M	0.603	0.98
59_P	62_V	0.603	0.98
286_L	308_V	0.602	0.98
156_V	285_V	0.602	0.98
52_K	216_G	0.600	0.98
19_L	316_D	0.598	0.98
252_A	255_D	0.598	0.98
219_E	290_G	0.597	0.98
205_D	209_E	0.596	0.98
224_S	227_M	0.594	0.98
119_V	122_A	0.592	0.98
79_T	82_M	0.591	0.98
222_E	227_M	0.587	0.98
83_M	123_T	0.587	0.98
183_A	186_E	0.586	0.98
153_G	168_I	0.583	0.98
224_S	247_K	0.582	0.98
199_Y	248_E	0.580	0.98
35_V	82_M	0.578	0.98
190_A	252_A	0.577	0.97
118_A	121_D	0.576	0.97
260_I	296_L	0.576	0.97
159_I	291_A	0.574	0.97
237_G	240_K	0.573	0.97
56_G	209_E	0.572	0.97
34_V	51_A	0.571	0.97
213_M	217_S	0.571	0.97
191_I	249_I	0.570	0.97
284_I	300_I	0.569	0.97
268_L	271_T	0.569	0.97
74_A	82_M	0.568	0.97
212_K	215_I	0.568	0.97
64_A	67_P	0.565	0.97
188_D	212_K	0.564	0.97
82_M	86_Y	0.563	0.97
207_T	210_A	0.562	0.97
246_G	249_I	0.561	0.97
225_D	294_R	0.560	0.97
68_M	118_A	0.559	0.97
195_I	199_Y	0.558	0.97
76_Y	122_A	0.556	0.97
264_V	304_T	0.556	0.97
197_K	201_L	0.556	0.97
188_D	219_E	0.556	0.97
257_V	293_L	0.554	0.97
250_S	257_V	0.553	0.97
65_L	162_G	0.553	0.97
206_R	239_P	0.552	0.97
210_A	231_G	0.552	0.97
263_A	266_S	0.551	0.97
23_K	323_G	0.549	0.97
210_A	233_D	0.549	0.97
249_I	252_A	0.548	0.97
110_G	179_S	0.546	0.97
108_P	111_I	0.546	0.97
10_G	106_C	0.546	0.97
151_P	170_S	0.543	0.96
263_A	268_L	0.543	0.96
199_Y	235_L	0.543	0.96
148_V	151_P	0.542	0.96
300_I	304_T	0.541	0.96
83_M	119_V	0.540	0.96
261_V	302_E	0.540	0.96
284_I	306_M	0.540	0.96
45_V	84_K	0.538	0.96
177_S	180_I	0.537	0.96
256_T	259_T	0.537	0.96
161_G	184_G	0.537	0.96
276_A	305_K	0.536	0.96
28_V	320_I	0.535	0.96
250_S	293_L	0.535	0.96
199_Y	240_K	0.534	0.96
11_I	105_V	0.533	0.96
202_M	232_R	0.533	0.96
156_V	287_T	0.533	0.96
183_A	290_G	0.533	0.96
63_V	68_M	0.529	0.96
69_K	118_A	0.529	0.96
33_S	64_A	0.529	0.96
124_R	130_D	0.527	0.96
155_M	304_T	0.527	0.96
58_T	64_A	0.527	0.96
186_E	189_D	0.525	0.96
64_A	70_D	0.525	0.96
50_D	89_Q	0.525	0.96
10_G	326_L	0.524	0.96
301_S	309_L	0.524	0.96
156_V	161_G	0.524	0.96
154_S	281_D	0.520	0.95
265_K	304_T	0.520	0.95
119_V	133_P	0.519	0.95
230_R	234_L	0.518	0.95
297_D	311_A	0.518	0.95
46_A	49_N	0.517	0.95
190_A	194_Y	0.516	0.95
13_L	82_M	0.514	0.95
253_L	290_G	0.514	0.95
209_E	213_M	0.512	0.95
106_C	138_F	0.512	0.95
178_Q	270_K	0.510	0.95
195_I	211_I	0.510	0.95
27_I	93_N	0.508	0.95
207_T	211_I	0.506	0.95
287_T	318_V	0.506	0.95
281_D	285_V	0.506	0.95
166_V	264_V	0.506	0.95
102_Y	132_Y	0.502	0.94
265_K	276_A	0.502	0.94
187_M	252_A	0.501	0.94
136_E	162_G	0.500	0.94
110_G	165_E	0.500	0.94
143_G	285_V	0.500	0.94

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4jneA	2	0.9674	100	0.239	Contact Map
4fl9A	1	0.9466	100	0.242	Contact Map
3d2fA	1	0.9585	100	0.249	Contact Map
2v7yA	1	0.9644	100	0.253	Contact Map
4rtfD	1	0.9347	100	0.265	Contact Map
3i33A	1	0.9377	100	0.273	Contact Map
4kboA	1	0.9407	100	0.284	Contact Map
4j8fA	2	0.9644	100	0.307	Contact Map
4gniA	2	0.9377	100	0.321	Contact Map
1jceA	1	0.9496	100	0.367	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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