MIND - BACSU - GREMLIN Contacts

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MIND - Septum site-determining protein MinD

UniProt:	Q01464	Sequences: MGEAIVITSGKGGVGKTTTSANLGTALAILGKRVCLVDTDIGLRNLDVVMGLENRIIYDLVDVVEGRCKMHQALVKDKRFDDLLYLMPAAQTSDKTAVAPEQIKNMVQELKQEFDYVIIDCPAGIEQGYKNAVSGADKAIVVTTPEISAVRDADRIIGLLEQEENIEPPRLVVNRIRNHLMKNGDTMDIDEIVQHLSIDLLGIVADDDEVIKASNHGEPIAMDPKNRASIAYRNIARRILGESVPLQVLEEQNKGMMAKIKSFFGVRS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	268 (232)
Sequences:	17016 (11090)
Seq/√Len:	728.1

ECOLI - MIND BCSQ MRP
BACSU - MIND SOJ YLXH SALA YWQD YVEL
HALSA - Q9HNF1 Q9HQY5 Q9HQJ7 Q9HHZ7 O51969 Q9HHU2 O54573 O51991 Q9HHL5
SULSO - Q97ZW4 Q981B3 Q97VX7 Q97WA2 Q97YM8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
205_A	231_A	3.942	1.00
85_Y	114_F	3.804	1.00
20_S	118_I	3.738	1.00
30_L	237_R	3.164	1.00
32_K	116_Y	3.038	1.00
6_V	136_A	2.877	1.00
27_L	116_Y	2.865	1.00
3_E	138_K	2.817	1.00
19_T	232_Y	2.788	1.00
144_T	175_R	2.759	1.00
36_L	46_L	2.652	1.00
35_C	114_F	2.619	1.00
175_R	206_D	2.531	1.00
21_A	46_L	2.513	1.00
180_L	183_N	2.259	1.00
24_G	36_L	2.182	1.00
49_V	148_S	2.116	1.00
19_T	172_V	2.103	1.00
209_E	212_K	2.095	1.00
76_K	85_Y	2.065	1.00
7_I	23_L	2.056	1.00
29_I	233_R	2.025	1.00
26_A	221_A	1.982	1.00
177_R	206_D	1.976	1.00
26_A	236_A	1.970	1.00
33_R	115_D	1.952	1.00
48_V	151_R	1.937	1.00
20_S	36_L	1.933	1.00
190_D	194_Q	1.903	1.00
172_V	235_I	1.851	1.00
27_L	118_I	1.850	1.00
138_K	169_P	1.742	1.00
39_T	132_A	1.719	1.00
21_A	49_V	1.704	1.00
85_Y	88_P	1.679	1.00
4_A	119_I	1.646	1.00
30_L	233_R	1.627	1.00
24_G	118_I	1.620	1.00
6_V	121_C	1.616	1.00
178_N	187_M	1.610	1.00
32_K	115_D	1.603	1.00
32_K	240_L	1.584	1.00
71_H	113_E	1.578	1.00
207_D	228_A	1.578	1.00
7_I	140_I	1.560	1.00
39_T	60_L	1.534	1.00
92_T	95_K	1.529	1.00
176_I	203_I	1.461	1.00
23_L	239_I	1.461	1.00
190_D	203_I	1.444	1.00
191_E	195_H	1.437	1.00
59_D	62_D	1.428	1.00
91_Q	94_D	1.427	1.00
185_D	188_D	1.416	1.00
95_K	99_A	1.414	1.00
138_K	170_R	1.410	1.00
21_A	50_M	1.405	1.00
184_G	188_D	1.400	1.00
202_G	238_R	1.393	1.00
173_V	189_I	1.392	1.00
140_I	172_V	1.390	1.00
39_T	119_I	1.388	1.00
183_N	187_M	1.348	1.00
23_L	236_A	1.340	1.00
45_N	152_D	1.339	1.00
24_G	46_L	1.334	1.00
3_E	137_D	1.332	1.00
170_R	201_L	1.323	1.00
145_P	173_V	1.321	1.00
208_D	212_K	1.306	1.00
15_G	174_N	1.280	1.00
139_A	160_L	1.274	1.00
230_I	233_R	1.266	1.00
35_C	117_V	1.265	1.00
8_T	156_I	1.260	1.00
7_I	20_S	1.257	1.00
226_N	229_S	1.253	1.00
202_G	235_I	1.248	1.00
146_E	149_A	1.243	1.00
19_T	174_N	1.236	1.00
27_L	34_V	1.214	1.00
192_I	196_L	1.207	1.00
30_L	240_L	1.203	1.00
209_E	223_D	1.196	1.00
17_T	49_V	1.191	1.00
91_Q	95_K	1.184	1.00
58_Y	62_D	1.175	1.00
204_V	228_A	1.164	1.00
98_V	102_Q	1.160	1.00
50_M	84_L	1.151	1.00
28_A	84_L	1.146	1.00
210_V	228_A	1.133	1.00
218_E	222_M	1.129	1.00
207_D	226_N	1.107	1.00
92_T	96_T	1.102	1.00
25_T	29_I	1.093	1.00
70_M	87_M	1.092	1.00
8_T	121_C	1.089	1.00
68_C	72_Q	1.089	1.00
61_V	65_E	1.078	1.00
44_R	47_D	1.072	1.00
212_K	216_H	1.067	1.00
231_A	234_N	1.066	1.00
200_L	203_I	1.062	1.00
234_N	237_R	1.053	1.00
45_N	148_S	1.047	1.00
22_N	25_T	1.047	1.00
94_D	97_A	1.043	1.00
5_I	116_Y	1.041	1.00
233_R	237_R	1.038	1.00
103_I	106_M	1.035	1.00
17_T	46_L	1.030	1.00
26_A	233_R	1.022	1.00
191_E	194_Q	1.014	1.00
116_Y	240_L	1.011	1.00
184_G	187_M	1.010	1.00
27_L	236_A	1.009	1.00
22_N	221_A	1.002	1.00
148_S	214_S	1.001	1.00
138_K	167_E	0.993	1.00
146_E	211_I	0.991	1.00
98_V	101_E	0.991	1.00
93_S	96_T	0.987	1.00
71_H	74_L	0.985	1.00
104_K	108_Q	0.978	1.00
237_R	241_G	0.977	1.00
19_T	23_L	0.974	1.00
96_T	100_P	0.967	1.00
18_T	210_V	0.963	1.00
84_L	88_P	0.953	1.00
26_A	30_L	0.951	1.00
69_K	72_Q	0.945	1.00
142_V	174_N	0.939	1.00
58_Y	68_C	0.933	1.00
95_K	100_P	0.933	1.00
4_A	117_V	0.923	1.00
33_R	113_E	0.922	1.00
99_A	102_Q	0.918	1.00
121_C	132_A	0.910	1.00
234_N	238_R	0.906	1.00
7_I	19_T	0.902	1.00
72_Q	75_V	0.897	1.00
177_R	186_T	0.896	1.00
228_A	231_A	0.896	1.00
8_T	129_Y	0.894	1.00
37_V	119_I	0.893	1.00
154_D	195_H	0.893	1.00
173_V	176_I	0.886	1.00
60_L	63_V	0.885	1.00
4_A	111_K	0.885	1.00
18_T	214_S	0.883	1.00
41_I	128_G	0.883	1.00
63_V	73_A	0.882	1.00
174_N	232_Y	0.881	1.00
224_P	229_S	0.879	1.00
93_S	99_A	0.875	1.00
106_M	109_E	0.870	1.00
25_T	221_A	0.870	1.00
71_H	75_V	0.862	1.00
221_A	233_R	0.851	1.00
29_I	221_A	0.849	1.00
141_V	157_I	0.848	1.00
73_A	87_M	0.845	1.00
5_I	239_I	0.838	1.00
207_D	227_R	0.835	1.00
158_G	161_E	0.834	1.00
229_S	233_R	0.833	1.00
235_I	239_I	0.832	1.00
91_Q	96_T	0.827	1.00
94_D	98_V	0.826	1.00
105_N	109_E	0.826	1.00
103_I	107_V	0.823	1.00
221_A	229_S	0.816	1.00
220_I	229_S	0.810	1.00
130_K	159_L	0.808	1.00
37_V	117_V	0.805	1.00
26_A	29_I	0.804	1.00
14_V	149_A	0.803	1.00
93_S	97_A	0.803	1.00
97_A	101_E	0.801	1.00
3_E	169_P	0.801	1.00
28_A	82_D	0.801	1.00
204_V	231_A	0.801	1.00
94_D	102_Q	0.800	1.00
39_T	128_G	0.798	1.00
5_I	138_K	0.798	1.00
91_Q	100_P	0.795	1.00
91_Q	99_A	0.792	1.00
42_G	126_E	0.781	1.00
7_I	118_I	0.775	1.00
216_H	222_M	0.770	1.00
145_P	176_I	0.769	1.00
144_T	149_A	0.769	1.00
48_V	147_I	0.765	1.00
10_G	159_L	0.764	1.00
24_G	84_L	0.763	1.00
141_V	153_A	0.758	1.00
34_V	116_Y	0.757	1.00
189_I	193_V	0.756	1.00
45_N	151_R	0.753	1.00
25_T	219_P	0.747	1.00
180_L	185_D	0.742	1.00
4_A	137_D	0.741	1.00
94_D	99_A	0.736	1.00
183_N	186_T	0.735	1.00
173_V	203_I	0.731	1.00
44_R	89_A	0.728	1.00
193_V	202_G	0.728	1.00
36_L	50_M	0.727	1.00
227_R	230_I	0.721	1.00
94_D	100_P	0.720	1.00
72_Q	76_K	0.710	1.00
114_F	117_V	0.702	1.00
201_L	238_R	0.696	1.00
154_D	158_G	0.695	1.00
22_N	232_Y	0.693	1.00
26_A	232_Y	0.693	1.00
169_P	201_L	0.690	1.00
19_T	142_V	0.689	1.00
111_K	137_D	0.689	1.00
132_A	136_A	0.688	1.00
170_R	199_D	0.688	1.00
69_K	109_E	0.686	1.00
121_C	129_Y	0.684	1.00
101_E	104_K	0.682	1.00
230_I	234_N	0.680	1.00
81_D	84_L	0.674	1.00
65_E	69_K	0.672	1.00
140_I	239_I	0.667	1.00
95_K	102_Q	0.667	1.00
91_Q	98_V	0.661	1.00
180_L	208_D	0.653	1.00
77_D	80_F	0.650	1.00
106_M	110_L	0.648	1.00
92_T	97_A	0.645	1.00
223_D	226_N	0.643	1.00
143_T	171_L	0.643	1.00
141_V	156_I	0.639	1.00
22_N	219_P	0.639	1.00
74_L	78_K	0.637	1.00
35_C	74_L	0.635	1.00
39_T	103_I	0.634	1.00
126_E	129_Y	0.633	1.00
73_A	76_K	0.633	1.00
232_Y	235_I	0.632	1.00
20_S	46_L	0.631	1.00
225_K	230_I	0.630	1.00
199_D	238_R	0.627	1.00
34_V	118_I	0.623	1.00
190_D	193_V	0.622	1.00
84_L	89_A	0.621	1.00
118_I	239_I	0.619	1.00
62_D	68_C	0.618	1.00
220_I	228_A	0.617	1.00
93_S	98_V	0.616	1.00
34_V	115_D	0.612	1.00
157_I	161_E	0.612	1.00
97_A	102_Q	0.611	1.00
72_Q	77_D	0.611	1.00
74_L	85_Y	0.610	1.00
60_L	87_M	0.607	1.00
3_E	32_K	0.606	1.00
11_K	148_S	0.606	1.00
16_K	123_A	0.605	1.00
193_V	203_I	0.604	1.00
227_R	231_A	0.603	1.00
9_S	14_V	0.603	1.00
58_Y	73_A	0.602	1.00
11_K	16_K	0.602	1.00
70_M	73_A	0.602	1.00
59_D	90_A	0.601	1.00
95_K	101_E	0.601	1.00
238_R	241_G	0.601	1.00
62_D	67_R	0.598	1.00
63_V	66_G	0.596	1.00
19_T	204_V	0.595	1.00
119_I	132_A	0.594	1.00
80_F	85_Y	0.594	1.00
52_L	86_L	0.594	1.00
147_I	151_R	0.592	1.00
31_G	82_D	0.592	1.00
47_D	84_L	0.589	1.00
93_S	102_Q	0.588	1.00
92_T	100_P	0.588	1.00
95_K	98_V	0.587	1.00
192_I	195_H	0.584	1.00
5_I	169_P	0.583	1.00
84_L	90_A	0.581	1.00
26_A	229_S	0.580	1.00
62_D	65_E	0.576	1.00
66_G	69_K	0.574	1.00
171_L	193_V	0.574	1.00
82_D	85_Y	0.574	1.00
108_Q	111_K	0.574	1.00
158_G	162_Q	0.573	1.00
212_K	222_M	0.572	1.00
68_C	73_A	0.570	1.00
155_R	158_G	0.568	1.00
211_I	214_S	0.566	1.00
213_A	220_I	0.565	1.00
125_I	159_L	0.563	1.00
81_D	85_Y	0.563	1.00
45_N	48_V	0.561	1.00
205_A	227_R	0.561	1.00
236_A	240_L	0.554	1.00
186_T	190_D	0.554	1.00
44_R	90_A	0.551	1.00
133_V	139_A	0.547	1.00
49_V	214_S	0.546	1.00
99_A	128_G	0.543	1.00
92_T	102_Q	0.543	1.00
21_A	36_L	0.542	1.00
75_V	78_K	0.541	1.00
96_T	99_A	0.540	1.00
208_D	211_I	0.540	1.00
147_I	215_N	0.537	1.00
138_K	168_P	0.537	1.00
11_K	14_V	0.536	1.00
23_L	235_I	0.536	1.00
25_T	28_A	0.535	1.00
179_H	186_T	0.534	1.00
6_V	132_A	0.534	1.00
77_D	81_D	0.533	1.00
184_G	189_I	0.527	1.00
137_D	165_N	0.527	1.00
62_D	66_G	0.525	1.00
97_A	100_P	0.522	1.00
63_V	106_M	0.522	1.00
87_M	90_A	0.520	1.00
47_D	88_P	0.519	1.00
213_A	219_P	0.516	1.00
28_A	34_V	0.516	1.00
100_P	131_N	0.516	1.00
183_N	189_I	0.514	1.00
33_R	114_F	0.514	1.00
187_M	190_D	0.513	1.00
4_A	136_A	0.512	1.00
29_I	224_P	0.512	1.00
143_T	153_A	0.508	1.00
101_E	105_N	0.506	1.00
48_V	148_S	0.506	1.00
131_N	134_S	0.505	1.00
104_K	135_G	0.504	0.99
96_T	102_Q	0.504	0.99
154_D	196_L	0.504	0.99
22_N	220_I	0.503	0.99
176_I	186_T	0.503	0.99
209_E	226_N	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1hyqA	1	0.8582	100	0.295	Contact Map
3q9lA	2	0.9291	100	0.298	Contact Map
4v03A	2	0.8769	100	0.299	Contact Map
4pfsA	1	0.8657	100	0.3	Contact Map
4rz3A	2	0.8806	100	0.307	Contact Map
1g3qA	1	0.8769	100	0.315	Contact Map
3fwyA	2	0.9291	100	0.335	Contact Map
3ea0A	2	0.8657	100	0.337	Contact Map
3pg5A	2	0.806	100	0.339	Contact Map
1wcv1	2	0.8657	100	0.341	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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