YWQG - BACSU - GREMLIN Contacts

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YWQG - Uncharacterized protein YwqG

UniProt:	P96719	Sequences: MNHLPEKMRPYRDLLEKSAKEYVKLNVRKGKTGRYDSKIAGDPYFPKHETYPTDENGQPMKLLAQINFSHIPQLDGYPSSGILQFYISVHDDVYGLNFDDRCEQKNFRVIYFENIVENDDELVSDFSFIGTGECDFPILSEAAVEPVKSSEWVLPTDFQFEQYTGMETMEFFGQFGEDEEDIYNELAENGFGHKIGGYASFTQHDPREYAYKEHTIMLLQIDSDDDIDSMWGDVGIANFFITPEDLRKKDFSNVLYNWDCS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	261 (237)
Sequences:	599 (525)
Seq/√Len:	34.1

BACSU - YWQG

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
193_H	224_D	4.161	1.00
25_L	146_P	3.759	1.00
85_F	109_V	3.660	1.00
151_E	194_K	3.503	1.00
35_Y	44_Y	3.092	1.00
217_M	220_Q	3.086	1.00
86_Y	112_F	3.068	1.00
242_T	245_D	3.064	1.00
85_F	239_F	2.845	1.00
223_S	231_W	2.772	1.00
241_I	246_L	2.573	1.00
245_D	250_D	2.559	1.00
238_N	259_D	2.549	1.00
36_D	70_H	2.531	1.00
113_E	249_K	2.507	1.00
84_Q	112_F	2.474	1.00
45_F	49_E	2.458	1.00
51_Y	86_Y	2.413	1.00
245_D	253_N	2.406	1.00
54_D	88_S	2.331	0.99
18_S	153_V	2.291	0.99
77_Y	196_G	2.244	0.99
25_L	39_I	2.234	0.99
63_L	239_F	2.215	0.99
67_N	70_H	2.199	0.99
215_T	242_T	2.195	0.99
217_M	238_N	2.125	0.99
63_L	221_I	2.082	0.99
202_T	259_D	2.056	0.98
22_Y	194_K	1.942	0.98
37_S	65_Q	1.913	0.97
223_S	233_D	1.896	0.97
44_Y	84_Q	1.863	0.97
104_Q	108_R	1.851	0.97
232_G	260_C	1.789	0.96
37_S	146_P	1.780	0.96
230_M	233_D	1.780	0.96
74_L	77_Y	1.777	0.96
203_Q	235_G	1.762	0.96
69_S	80_S	1.750	0.96
230_M	234_V	1.749	0.96
39_I	221_I	1.708	0.95
33_G	36_D	1.666	0.94
214_H	253_N	1.648	0.94
27_V	144_V	1.645	0.94
60_M	108_R	1.612	0.93
66_I	195_I	1.590	0.93
222_D	236_I	1.573	0.92
223_S	230_M	1.563	0.92
68_F	71_I	1.559	0.92
39_I	144_V	1.556	0.92
19_A	150_S	1.530	0.91
203_Q	260_C	1.521	0.91
111_Y	249_K	1.513	0.91
216_I	243_P	1.504	0.90
51_Y	59_P	1.502	0.90
68_F	111_Y	1.483	0.90
201_F	205_D	1.462	0.89
51_Y	123_V	1.453	0.89
12_R	16_E	1.447	0.89
22_Y	149_S	1.446	0.89
27_V	39_I	1.432	0.88
97_N	101_R	1.420	0.88
222_D	235_G	1.390	0.87
63_L	233_D	1.386	0.86
65_Q	84_Q	1.384	0.86
45_F	112_F	1.382	0.86
246_L	251_F	1.379	0.86
45_F	51_Y	1.369	0.86
220_Q	232_G	1.351	0.85
72_P	196_G	1.347	0.85
64_A	85_F	1.334	0.84
74_L	196_G	1.325	0.84
38_K	44_Y	1.323	0.84
142_A	227_I	1.318	0.83
67_N	82_I	1.301	0.83
68_F	86_Y	1.290	0.82
199_A	205_D	1.285	0.82
250_D	253_N	1.281	0.82
46_P	68_F	1.277	0.81
193_H	220_Q	1.261	0.81
44_Y	65_Q	1.236	0.79
87_I	259_D	1.231	0.79
66_I	146_P	1.226	0.79
54_D	58_Q	1.217	0.78
68_F	85_F	1.209	0.78
43_P	86_Y	1.199	0.77
203_Q	220_Q	1.193	0.77
95_G	256_Y	1.161	0.75
21_E	75_D	1.128	0.73
215_T	243_P	1.110	0.71
178_D	184_N	1.105	0.71
194_K	217_M	1.098	0.70
240_F	259_D	1.093	0.70
96_L	258_W	1.088	0.70
65_Q	219_L	1.087	0.70
20_K	198_Y	1.082	0.69
202_T	238_N	1.077	0.69
87_I	238_N	1.075	0.69
63_L	87_I	1.065	0.68
22_Y	151_E	1.064	0.68
255_L	258_W	1.062	0.68
71_I	248_K	1.053	0.67
84_Q	115_I	1.052	0.67
144_V	195_I	1.049	0.67
46_P	112_F	1.049	0.67
21_E	148_K	1.047	0.67
231_W	237_A	1.039	0.66
40_A	77_Y	1.038	0.66
192_G	222_D	1.036	0.66
84_Q	196_G	1.034	0.66
230_M	260_C	1.033	0.66
27_V	221_I	1.029	0.65
40_A	48_H	1.019	0.65
23_V	195_I	1.017	0.64
203_Q	259_D	1.016	0.64
40_A	199_A	1.015	0.64
66_I	71_I	1.014	0.64
44_Y	67_N	1.013	0.64
64_A	220_Q	1.005	0.64
59_P	108_R	0.987	0.62
36_D	67_N	0.971	0.61
220_Q	239_F	0.968	0.61
34_R	199_A	0.964	0.60
45_F	70_H	0.953	0.59
169_M	173_G	0.947	0.59
59_P	86_Y	0.939	0.58
49_E	112_F	0.929	0.57
26_N	145_E	0.920	0.57
178_D	185_E	0.917	0.56
243_P	247_R	0.911	0.56
229_S	237_A	0.909	0.56
12_R	20_K	0.899	0.55
217_M	222_D	0.898	0.55
35_Y	84_Q	0.892	0.54
217_M	240_F	0.890	0.54
43_P	62_L	0.888	0.54
220_Q	235_G	0.884	0.54
223_S	235_G	0.883	0.54
248_K	252_S	0.881	0.53
118_N	121_E	0.881	0.53
62_L	65_Q	0.878	0.53
174_Q	185_E	0.876	0.53
37_S	70_H	0.874	0.53
201_F	207_R	0.873	0.53
46_P	110_I	0.868	0.52
241_I	251_F	0.863	0.52
156_T	188_E	0.863	0.52
245_D	252_S	0.855	0.51
68_F	77_Y	0.851	0.51
45_F	86_Y	0.848	0.51
151_E	154_L	0.847	0.50
233_D	260_C	0.847	0.50
183_Y	188_E	0.846	0.50
48_H	247_R	0.838	0.50
220_Q	260_C	0.834	0.49
71_I	146_P	0.830	0.49
143_A	146_P	0.829	0.49
32_T	38_K	0.826	0.49
13_D	244_E	0.823	0.48
203_Q	217_M	0.822	0.48
68_F	107_F	0.819	0.48
66_I	219_L	0.818	0.48
35_Y	86_Y	0.817	0.48
83_L	86_Y	0.816	0.48
84_Q	201_F	0.815	0.48
69_S	250_D	0.812	0.48
89_V	138_I	0.808	0.47
93_V	96_L	0.796	0.46
77_Y	215_T	0.791	0.46
239_F	254_V	0.780	0.45
25_L	165_G	0.777	0.45
13_D	169_M	0.773	0.44
25_L	71_I	0.763	0.43
234_V	260_C	0.763	0.43
223_S	232_G	0.752	0.43
82_I	112_F	0.746	0.42
71_I	97_N	0.743	0.42
145_E	163_Y	0.739	0.41
54_D	106_N	0.738	0.41
15_L	24_K	0.732	0.41
199_A	259_D	0.730	0.41
37_S	219_L	0.729	0.41
80_S	113_E	0.729	0.41
29_K	113_E	0.728	0.41
76_G	243_P	0.723	0.40
201_F	238_N	0.719	0.40
77_Y	216_I	0.718	0.40
55_E	106_N	0.715	0.39
69_S	178_D	0.711	0.39
167_E	185_E	0.711	0.39
17_K	221_I	0.710	0.39
118_N	254_V	0.710	0.39
240_F	257_N	0.710	0.39
160_F	203_Q	0.709	0.39
85_F	230_M	0.709	0.39
138_I	142_A	0.708	0.39
74_L	86_Y	0.707	0.39
45_F	61_K	0.706	0.39
97_N	103_E	0.706	0.39
136_F	159_Q	0.700	0.38
25_L	32_T	0.699	0.38
56_N	105_K	0.698	0.38
240_F	256_Y	0.697	0.38
72_P	77_Y	0.696	0.38
44_Y	115_I	0.694	0.38
50_T	120_D	0.694	0.38
162_Q	184_N	0.693	0.38
87_I	237_A	0.691	0.38
104_Q	107_F	0.691	0.38
39_I	216_I	0.688	0.37
44_Y	112_F	0.688	0.37
60_M	250_D	0.687	0.37
220_Q	238_N	0.679	0.37
68_F	196_G	0.675	0.36
215_T	245_D	0.671	0.36
24_K	149_S	0.668	0.36
54_D	237_A	0.667	0.36
238_N	251_F	0.665	0.36
74_L	111_Y	0.664	0.35
15_L	37_S	0.660	0.35
161_E	186_L	0.660	0.35
23_V	46_P	0.658	0.35
92_D	260_C	0.655	0.35
11_Y	15_L	0.653	0.35
241_I	244_E	0.652	0.35
49_E	110_I	0.651	0.34
34_R	159_Q	0.649	0.34
77_Y	153_V	0.646	0.34
27_V	224_D	0.644	0.34
202_T	205_D	0.643	0.34
50_T	176_G	0.642	0.34
69_S	72_P	0.639	0.34
186_L	244_E	0.638	0.33
236_I	257_N	0.636	0.33
212_K	215_T	0.635	0.33
41_G	52_P	0.634	0.33
60_M	135_D	0.631	0.33
197_G	220_Q	0.631	0.33
88_S	106_N	0.629	0.33
43_P	118_N	0.628	0.33
80_S	115_I	0.624	0.32
47_K	121_E	0.623	0.32
116_V	141_E	0.620	0.32
112_F	115_I	0.619	0.32
118_N	225_D	0.619	0.32
38_K	198_Y	0.618	0.32
199_A	217_M	0.616	0.32
27_V	193_H	0.615	0.32
38_K	141_E	0.614	0.32
83_L	112_F	0.613	0.32
169_M	175_F	0.612	0.32
47_K	125_D	0.607	0.31
92_D	96_L	0.604	0.31
110_I	211_Y	0.603	0.31
15_L	59_P	0.603	0.31
202_T	220_Q	0.602	0.31
214_H	255_L	0.601	0.31
43_P	65_Q	0.599	0.31
243_P	252_S	0.597	0.30
193_H	227_I	0.596	0.30
46_P	78_P	0.594	0.30
23_V	196_G	0.594	0.30
65_Q	70_H	0.592	0.30
60_M	88_S	0.590	0.30
80_S	111_Y	0.590	0.30
28_R	143_A	0.589	0.30
77_Y	195_I	0.587	0.30
253_N	257_N	0.585	0.30
21_E	150_S	0.585	0.30
235_G	238_N	0.585	0.30
163_Y	186_L	0.584	0.30
45_F	237_A	0.580	0.29
11_Y	97_N	0.580	0.29
103_E	252_S	0.578	0.29
65_Q	82_I	0.576	0.29
95_G	101_R	0.576	0.29
238_N	258_W	0.574	0.29
68_F	246_L	0.574	0.29
29_K	79_S	0.573	0.29
141_E	248_K	0.573	0.29
111_Y	239_F	0.572	0.29
96_L	101_R	0.572	0.29
14_L	165_G	0.570	0.29
238_N	257_N	0.563	0.28
109_V	239_F	0.562	0.28
201_F	206_P	0.562	0.28
14_L	17_K	0.561	0.28
86_Y	123_V	0.559	0.28
84_Q	114_N	0.559	0.28
107_F	239_F	0.557	0.28
21_E	72_P	0.557	0.28
202_T	236_I	0.556	0.28
64_A	251_F	0.556	0.28
144_V	219_L	0.554	0.27
237_A	254_V	0.554	0.27
193_H	221_I	0.552	0.27
201_F	240_F	0.550	0.27
176_G	181_D	0.547	0.27
220_Q	240_F	0.546	0.27
238_N	260_C	0.545	0.27
110_I	219_L	0.545	0.27
64_A	239_F	0.542	0.27
51_Y	61_K	0.542	0.27
48_H	121_E	0.541	0.27
12_R	23_V	0.541	0.27
257_N	260_C	0.541	0.27
86_Y	173_G	0.541	0.27
56_N	156_T	0.540	0.27
77_Y	152_W	0.540	0.27
77_Y	226_D	0.540	0.27
57_G	85_F	0.539	0.26
241_I	245_D	0.538	0.26
51_Y	62_L	0.537	0.26
55_E	213_E	0.536	0.26
31_K	55_E	0.535	0.26
76_G	247_R	0.535	0.26
207_R	232_G	0.533	0.26
103_E	164_T	0.533	0.26
219_L	250_D	0.533	0.26
224_D	227_I	0.532	0.26
229_S	260_C	0.530	0.26
28_R	162_Q	0.530	0.26
82_I	103_E	0.529	0.26
53_T	59_P	0.527	0.26
39_I	142_A	0.525	0.26
203_Q	232_G	0.525	0.26
43_P	146_P	0.525	0.26
43_P	84_Q	0.524	0.25
179_E	182_I	0.524	0.25
236_I	240_F	0.523	0.25
39_I	226_D	0.523	0.25
47_K	118_N	0.522	0.25
51_Y	68_F	0.520	0.25
16_E	30_G	0.518	0.25
108_R	169_M	0.518	0.25
16_E	194_K	0.517	0.25
218_L	246_L	0.517	0.25
236_I	259_D	0.517	0.25
66_I	186_L	0.514	0.25
35_Y	118_N	0.514	0.25
37_S	68_F	0.510	0.25
65_Q	111_Y	0.510	0.25
206_P	210_A	0.509	0.25
178_D	183_Y	0.509	0.25
202_T	235_G	0.508	0.24
45_F	59_P	0.508	0.24
37_S	67_N	0.508	0.24
151_E	199_A	0.506	0.24
63_L	231_W	0.504	0.24
223_S	234_V	0.504	0.24
80_S	226_D	0.503	0.24
201_F	256_Y	0.503	0.24
164_T	256_Y	0.501	0.24
32_T	72_P	0.500	0.24
244_E	247_R	0.500	0.24

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1pv5A	1	0.9962	100	0.149	Contact Map
1j8bA	2	0.1533	8.9	0.962	Contact Map
3s9gA	2	0.1111	4.7	0.967	Contact Map
4lh9A	4	0.1533	4.2	0.967	Contact Map
2js3A	2	0.2797	4.1	0.968	Contact Map
4c1aA	2	0.1264	3.2	0.969	Contact Map
3m1fV	1	0.0843	3	0.97	Contact Map
1z8uA	1	0.0958	2.3	0.971	Contact Map
1mz9A	3	0.1341	2.2	0.972	Contact Map
3h6pC	1	0.0536	2.1	0.972	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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