GREMLIN Database
NICK - Putative DNA relaxase NicK
UniProt: P96635 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 352 (321)
Sequences: 298 (220)
Seq/√Len: 12.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
193_C221_R5.7551.00
150_K155_E5.6231.00
101_R104_E4.4211.00
296_S307_T3.9010.99
136_D215_W3.3750.98
194_F218_Y3.2930.98
196_E216_N3.1810.97
100_C114_W3.1780.97
226_R251_Y3.0890.97
311_V314_A3.0250.96
137_D141_Y2.6440.92
334_D337_K2.6360.92
142_F275_F2.5650.91
104_E114_W2.5320.90
35_I118_F2.5230.90
101_R114_W2.4890.89
73_Y95_M2.4510.89
137_D254_F2.3700.87
137_D275_F2.3690.87
180_T193_C2.3430.86
73_Y91_V2.3350.86
96_S193_C2.2210.83
300_L326_M2.2080.83
97_G100_C2.1320.80
36_R92_L2.1230.80
313_E316_E2.1040.79
38_S215_W2.1000.79
32_V35_I2.0890.79
34_Y193_C2.0440.77
163_S169_F1.9810.75
315_D320_K1.9450.73
78_I101_R1.8810.71
35_I54_L1.8800.71
137_D272_W1.8750.71
113_T116_D1.8690.70
201_Q223_K1.8600.70
34_Y248_I1.8390.69
34_Y94_E1.8260.68
198_N215_W1.7790.66
157_I253_R1.7610.66
192_L248_I1.7280.64
323_L326_M1.7210.64
156_C250_N1.6920.62
100_C104_E1.6900.62
133_L161_R1.6800.62
117_F181_I1.6550.61
190_A220_L1.6410.60
40_K129_T1.6290.59
195_Y219_E1.6220.59
195_Y217_R1.6220.59
178_G299_W1.6210.59
97_G104_E1.6130.58
304_C308_M1.6090.58
150_K299_W1.6020.58
57_D338_K1.5690.56
46_R50_E1.5680.56
172_S340_L1.5580.56
310_M340_L1.5340.55
155_E266_W1.5290.54
192_L220_L1.5150.54
150_K275_F1.5110.53
49_E131_F1.5080.53
40_K127_S1.5030.53
132_D195_Y1.4930.52
34_Y92_L1.4650.51
77_Y98_Q1.4460.50
292_F308_M1.4330.49
29_V118_F1.4170.49
107_L204_K1.4140.48
132_D217_R1.4120.48
132_D219_E1.4120.48
197_K217_R1.4070.48
197_K219_E1.4070.48
136_D214_D1.4070.48
201_Q288_P1.4010.48
90_G200_E1.3940.47
101_R272_W1.3810.47
137_D144_I1.3700.46
34_Y221_R1.3580.46
127_S136_D1.3540.45
168_S208_P1.3530.45
78_I97_G1.3500.45
102_Q106_F1.3440.45
38_S129_T1.3380.45
169_F192_L1.3360.44
300_L307_T1.3270.44
37_V93_I1.3230.44
306_P336_H1.3220.44
94_E193_C1.3200.44
305_A327_I1.3070.43
282_L315_D1.2970.43
131_F230_A1.2940.42
218_Y248_I1.2860.42
216_N299_W1.2860.42
97_G101_R1.2780.42
193_C248_I1.2770.42
132_D197_K1.2770.42
230_A247_I1.2650.41
32_V222_L1.2500.40
70_V99_G1.2450.40
136_D195_Y1.2330.39
28_L135_I1.2220.39
300_L311_V1.2070.38
202_A218_Y1.1990.38
194_F202_A1.1910.37
137_D299_W1.1900.37
189_E226_R1.1820.37
184_G195_Y1.1780.37
172_S311_V1.1750.37
321_T335_K1.1680.36
99_G245_M1.1670.36
34_Y272_W1.1640.36
186_K191_Y1.1630.36
77_Y102_Q1.1600.36
130_R195_Y1.1600.36
80_V93_I1.1480.35
144_I254_F1.1440.35
128_F154_G1.1250.34
115_Y175_I1.1230.34
69_Y197_K1.1210.34
191_Y220_L1.1180.34
294_Q334_D1.1170.34
107_L216_N1.1090.34
134_A222_L1.1040.33
34_Y295_K1.1010.33
47_I51_V1.0990.33
48_I54_L1.0980.33
184_G217_R1.0960.33
184_G219_E1.0960.33
98_Q201_Q1.0960.33
231_I234_L1.0930.33
147_L196_E1.0920.33
335_K344_M1.0910.33
136_D219_E1.0910.33
136_D217_R1.0910.33
29_V75_L1.0900.33
116_D288_P1.0900.33
59_M240_L1.0780.32
130_R217_R1.0760.32
130_R219_E1.0760.32
84_A209_L1.0750.32
54_L266_W1.0660.32
76_D166_N1.0610.31
192_L245_M1.0550.31
97_G134_A1.0470.31
133_L260_N1.0420.31
118_F303_S1.0360.30
93_I173_D1.0340.30
38_S92_L1.0330.30
189_E225_E1.0300.30
153_K257_A1.0200.30
103_F290_K1.0180.30
305_A330_A1.0170.30
258_D280_G1.0160.29
147_L341_D1.0140.29
253_R284_L1.0060.29
118_F252_V0.9990.29
33_D195_Y0.9960.29
137_D252_V0.9940.29
196_E199_Y0.9920.28
245_M275_F0.9920.28
53_H205_Y0.9900.28
151_A183_F0.9900.28
146_E271_F0.9880.28
272_W299_W0.9830.28
182_Y299_W0.9790.28
117_F201_Q0.9780.28
100_C284_L0.9680.28
236_K328_A0.9680.28
133_L226_R0.9660.27
99_G199_Y0.9650.27
34_Y192_L0.9640.27
161_R304_C0.9640.27
180_T223_K0.9610.27
131_F306_P0.9610.27
191_Y221_R0.9610.27
46_R61_E0.9600.27
65_G154_G0.9590.27
184_G197_K0.9590.27
66_F81_F0.9580.27
80_V189_E0.9530.27
133_L206_N0.9520.27
104_E272_W0.9510.27
132_D184_G0.9470.27
157_I255_V0.9460.27
48_I56_K0.9450.27
231_I235_L0.9430.26
103_F152_Q0.9430.26
130_R197_K0.9410.26
293_Y311_V0.9380.26
304_C307_T0.9380.26
32_V54_L0.9350.26
136_D197_K0.9320.26
210_E270_L0.9310.26
169_F322_D0.9300.26
222_L247_I0.9290.26
336_H340_L0.9220.26
36_R145_P0.9220.26
54_L106_F0.9140.25
190_A251_Y0.9100.25
125_G284_L0.9090.25
33_D217_R0.9040.25
33_D219_E0.9040.25
226_R285_Y0.8960.25
156_C171_L0.8900.24
287_K341_D0.8890.24
132_D136_D0.8840.24
32_V86_D0.8820.24
179_T196_E0.8780.24
125_G187_K0.8770.24
89_R177_G0.8720.24
196_E215_W0.8680.24
81_F194_F0.8680.24
30_S298_N0.8620.23
36_R277_G0.8610.23
38_S291_D0.8610.23
30_S136_D0.8580.23
199_Y210_E0.8570.23
202_A213_G0.8560.23
154_G174_G0.8530.23
68_G90_G0.8520.23
331_E340_L0.8510.23
191_Y314_A0.8440.23
167_G315_D0.8420.23
69_Y195_Y0.8420.23
157_I223_K0.8420.23
48_I222_L0.8410.23
313_E341_D0.8400.23
114_W272_W0.8380.23
82_Y136_D0.8310.22
333_A336_H0.8310.22
97_G272_W0.8260.22
102_Q289_Q0.8240.22
135_I294_Q0.8220.22
314_A344_M0.8190.22
257_A266_W0.8170.22
252_V282_L0.8040.21
39_F99_G0.8020.21
171_L249_N0.8010.21
160_F275_F0.7970.21
161_R323_L0.7970.21
292_F328_A0.7970.21
106_F154_G0.7970.21
94_E221_R0.7950.21
178_G308_M0.7930.21
157_I284_L0.7930.21
42_H246_Q0.7910.21
143_S149_K0.7910.21
113_T231_I0.7890.21
176_T340_L0.7890.21
237_T293_Y0.7880.21
68_G251_Y0.7870.21
96_S142_F0.7860.21
298_N329_E0.7840.21
149_K325_D0.7810.21
186_K223_K0.7740.20
272_W275_F0.7730.20
90_G201_Q0.7700.20
80_V149_K0.7680.20
102_Q213_G0.7640.20
194_F213_G0.7640.20
33_D197_K0.7640.20
171_L308_M0.7620.20
296_S339_M0.7580.20
78_I221_R0.7570.20
38_S228_Q0.7570.20
129_T136_D0.7560.20
33_D132_D0.7560.20
52_L200_E0.7560.20
39_F67_Y0.7560.20
39_F326_M0.7550.20
107_L121_C0.7520.20
32_V146_E0.7470.19
221_R266_W0.7460.19
36_R223_K0.7440.19
28_L244_A0.7410.19
61_E71_G0.7400.19
150_K216_N0.7400.19
129_T193_C0.7400.19
122_M335_K0.7400.19
153_K280_G0.7390.19
312_L327_I0.7370.19
97_G299_W0.7360.19
69_Y219_E0.7360.19
69_Y217_R0.7360.19
209_L244_A0.7360.19
54_L79_K0.7350.19
259_E345_A0.7340.19
129_T224_N0.7340.19
149_K236_K0.7330.19
107_L188_S0.7320.19
34_Y96_S0.7310.19
154_G321_T0.7300.19
32_V226_R0.7260.19
25_E327_I0.7250.19
82_Y129_T0.7240.19
191_Y295_K0.7240.19
229_V284_L0.7240.19
112_K302_N0.7200.19
79_K121_C0.7190.19
112_K120_D0.7180.19
131_F313_E0.7170.19
103_F118_F0.7160.19
136_D212_L0.7160.19
39_F93_I0.7140.18
102_Q199_Y0.7110.18
29_V49_E0.7070.18
255_V266_W0.7040.18
202_A216_N0.7030.18
99_G107_L0.7020.18
244_A300_L0.7010.18
221_R248_I0.7010.18
173_D334_D0.7000.18
38_S261_I0.7000.18
266_W275_F0.7000.18
87_D98_Q0.6980.18
206_N328_A0.6960.18
141_Y198_N0.6950.18
152_Q167_G0.6930.18
230_A234_L0.6930.18
77_Y300_L0.6870.18
178_G272_W0.6850.18
195_Y200_E0.6760.17
59_M340_L0.6750.17
138_K340_L0.6740.17
78_I282_L0.6720.17
252_V314_A0.6720.17
178_G247_I0.6720.17
34_Y291_D0.6710.17
187_K286_V0.6710.17
309_K330_A0.6640.17
107_L201_Q0.6620.17
69_Y340_L0.6600.17
160_F294_Q0.6580.17
179_T293_Y0.6580.17
228_Q232_D0.6540.17
160_F251_Y0.6530.17
37_V53_H0.6480.17
37_V121_C0.6470.16
149_K239_D0.6460.16
118_F158_S0.6440.16
98_Q327_I0.6420.16
186_K254_F0.6420.16
254_F275_F0.6410.16
333_A342_V0.6400.16
100_C272_W0.6380.16
299_W304_C0.6340.16
100_C134_A0.6330.16
90_G94_E0.6330.16
77_Y322_D0.6320.16
54_L75_L0.6320.16
121_C126_G0.6310.16
80_V337_K0.6300.16
50_E341_D0.6300.16
320_K335_K0.6290.16
93_I238_K0.6290.16
162_K177_G0.6270.16
161_R224_N0.6240.16
231_I259_E0.6230.16
293_Y322_D0.6220.16
258_D277_G0.6220.16
111_K206_N0.6210.16
340_L344_M0.6200.16
51_V80_V0.6200.16
65_G244_A0.6180.16
300_L323_L0.6180.16
176_T190_A0.6150.16
321_T325_D0.6130.16
334_D340_L0.6090.15
53_H110_R0.6090.15
97_G195_Y0.6080.15
270_L328_A0.6070.15
93_I314_A0.6060.15
292_F311_V0.6030.15
66_F335_K0.6020.15
75_L232_D0.6010.15
138_K244_A0.6010.15
136_D274_D0.6000.15
174_G218_Y0.6000.15
67_Y168_S0.6000.15
136_D139_K0.5970.15
221_R315_D0.5960.15
137_D266_W0.5960.15
184_G200_E0.5960.15
104_E200_E0.5950.15
112_K198_N0.5950.15
49_E312_L0.5930.15
143_S192_L0.5920.15
32_V50_E0.5900.15
150_K271_F0.5840.15
177_G199_Y0.5840.15
78_I104_E0.5820.15
137_D150_K0.5820.15
69_Y88_N0.5800.15
200_E219_E0.5790.15
200_E217_R0.5790.15
130_R184_G0.5780.15
101_R284_L0.5770.15
161_R256_D0.5750.15
135_I155_E0.5740.15
319_G344_M0.5740.15
40_K126_G0.5730.15
99_G266_W0.5730.15
334_D341_D0.5700.14
48_I261_I0.5680.14
218_Y299_W0.5660.14
83_S122_M0.5650.14
115_Y294_Q0.5640.14
76_D199_Y0.5640.14
124_Q284_L0.5640.14
137_D179_T0.5630.14
161_R191_Y0.5630.14
156_C310_M0.5620.14
282_L337_K0.5620.14
106_F281_R0.5610.14
142_F183_F0.5610.14
26_S273_S0.5600.14
99_G204_K0.5600.14
137_D188_S0.5590.14
196_E244_A0.5590.14
328_A344_M0.5590.14
69_Y132_D0.5570.14
45_D49_E0.5570.14
194_F216_N0.5560.14
104_E134_A0.5550.14
44_V56_K0.5550.14
131_F209_L0.5550.14
194_F198_N0.5540.14
160_F307_T0.5530.14
234_L252_V0.5530.14
230_A248_I0.5500.14
193_C223_K0.5480.14
41_T230_A0.5460.14
97_G220_L0.5450.14
173_D317_H0.5440.14
171_L196_E0.5430.14
311_V321_T0.5420.14
187_K279_V0.5410.14
221_R300_L0.5380.14
185_S305_A0.5380.14
58_F204_K0.5370.14
165_F183_F0.5360.14
133_L240_L0.5350.14
244_A321_T0.5350.14
42_H183_F0.5340.14
154_G310_M0.5340.14
113_T230_A0.5330.14
101_R200_E0.5320.14
85_P337_K0.5320.14
144_I147_L0.5310.13
179_T186_K0.5290.13
51_V165_F0.5290.13
136_D305_A0.5270.13
80_V90_G0.5260.13
121_C162_K0.5220.13
146_E149_K0.5200.13
33_D38_S0.5190.13
47_I52_L0.5170.13
259_E308_M0.5170.13
30_S135_I0.5170.13
151_A171_L0.5160.13
55_S239_D0.5140.13
255_V331_E0.5130.13
177_G276_I0.5120.13
173_D270_L0.5110.13
78_I100_C0.5110.13
254_F276_I0.5100.13
64_S223_K0.5090.13
99_G162_K0.5090.13
319_G346_D0.5070.13
183_F294_Q0.5070.13
289_Q306_P0.5060.13
142_F269_S0.5060.13
311_V325_D0.5060.13
97_G219_E0.5050.13
97_G217_R0.5050.13
142_F218_Y0.5040.13
125_G337_K0.5040.13
150_K256_D0.5040.13
92_L291_D0.5020.13
30_S232_D0.5010.13
80_V159_R0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4cijA 2 0.7244 100 0.37 Contact Map
4cwcA 2 0.7301 100 0.7 Contact Map
4nj6A 3 0.2557 6.4 0.975 Contact Map
2hyeC 1 0.196 6 0.976 Contact Map
2g31A 1 0.1705 5.1 0.976 Contact Map
2do7A 1 0.2131 4.4 0.977 Contact Map
1k8kF 1 0.3494 4.2 0.977 Contact Map
1ldjA 1 0.2017 3.5 0.978 Contact Map
4yvqA 2 0.1477 3.4 0.978 Contact Map
3dplC 1 0.2017 3.3 0.978 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0348 seconds.