FLGL - BACSU - GREMLIN Contacts

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FLGL - Flagellar hook-associated protein 3

UniProt:	P96501	Sequences: MRVTQGMIQQNSLRYIGSSYSKLDKLQSQISSGKKISKASDDPVVAMKSLKYNTQLSQVQQYKSNASQAFTWLENTETNITEGIDILSKVRELAVEAQNDTNGEPERQAIGVEVKQLKEQLLNIANTQVNGRYIFNGTNSDKPPVTDNGDGTYTISDTADVVVNISNNMSLKVNSDPKSAFGGTSESGQNVFEMLDSFEKALNSGSLDGMDSVLNDIDHFSDGMSAERSDLGARYNRLELVNTRLSAQEETATKVLSDNEDVELEEVITEFIAQQSVHRATLAVNAQIVQPTLIDFLK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	298 (284)
Sequences:	6388 (4835)
Seq/√Len:	286.9

ECOLI - FLGL FLIC
BACSU - FLGL FLA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
91_R	218_D	4.527	1.00
111_G	115_K	3.671	1.00
83_G	121_L	3.560	1.00
90_V	114_V	3.339	1.00
257_S	261_D	2.949	1.00
89_K	113_E	2.923	1.00
29_Q	267_V	2.860	1.00
63_K	246_S	2.713	1.00
60_Q	249_E	2.651	1.00
103_G	106_E	2.615	1.00
35_K	42_D	2.537	1.00
53_N	256_L	2.494	1.00
266_E	270_E	2.482	1.00
19_S	281_T	2.442	1.00
116_Q	119_E	2.416	1.00
61_Q	64_S	2.384	1.00
256_L	260_E	2.376	1.00
120_Q	123_N	2.362	1.00
262_V	267_V	2.344	1.00
34_K	260_E	2.331	1.00
86_I	121_L	2.328	1.00
86_I	117_L	2.305	1.00
66_A	242_N	2.276	1.00
36_I	45_V	2.246	1.00
57_S	92_E	2.146	1.00
26_L	274_Q	2.141	1.00
89_K	93_L	2.116	1.00
25_K	29_Q	2.046	1.00
77_E	228_R	2.026	1.00
87_L	217_I	2.010	1.00
104_E	108_Q	1.998	1.00
73_L	231_L	1.973	1.00
28_S	38_K	1.963	1.00
227_E	230_D	1.877	1.00
108_Q	112_V	1.836	1.00
90_V	117_L	1.834	1.00
49_S	260_E	1.831	1.00
142_K	145_V	1.814	1.00
93_L	113_E	1.774	1.00
53_N	260_E	1.757	1.00
94_A	214_L	1.748	1.00
115_K	203_N	1.734	1.00
218_D	222_D	1.729	1.00
82_E	85_D	1.692	1.00
89_K	92_E	1.672	1.00
83_G	224_M	1.650	1.00
87_L	224_M	1.635	1.00
100_D	107_R	1.591	1.00
87_L	221_S	1.544	1.00
100_D	207_L	1.538	1.00
143_P	146_T	1.536	1.00
139_N	142_K	1.522	1.00
42_D	45_V	1.521	1.00
110_I	114_V	1.518	1.00
86_I	124_I	1.512	1.00
109_A	112_V	1.488	1.00
111_G	114_V	1.463	1.00
93_L	110_I	1.459	1.00
110_I	204_S	1.458	1.00
59_V	245_L	1.453	1.00
45_V	259_N	1.453	1.00
115_K	119_E	1.446	1.00
90_V	217_I	1.446	1.00
83_G	86_I	1.428	1.00
26_L	270_E	1.426	1.00
115_K	118_K	1.420	1.00
112_V	230_D	1.404	1.00
86_I	120_Q	1.394	1.00
97_A	110_I	1.391	1.00
118_K	122_L	1.382	1.00
102_N	106_E	1.381	1.00
157_D	163_V	1.364	1.00
70_F	239_E	1.341	1.00
106_E	109_A	1.336	1.00
26_L	267_V	1.325	1.00
204_S	210_M	1.323	1.00
98_Q	211_D	1.322	1.00
36_I	49_S	1.310	1.00
79_N	128_Q	1.307	1.00
249_E	253_T	1.299	1.00
122_L	126_N	1.296	1.00
105_P	222_D	1.290	1.00
79_N	124_I	1.286	1.00
14_R	18_S	1.276	1.00
105_P	108_Q	1.269	1.00
272_I	275_Q	1.268	1.00
56_L	253_T	1.263	1.00
206_S	209_G	1.262	1.00
193_E	197_S	1.255	1.00
268_I	272_I	1.243	1.00
123_N	127_T	1.239	1.00
80_I	83_G	1.233	1.00
91_R	95_V	1.229	1.00
88_S	91_R	1.215	1.00
118_K	199_E	1.214	1.00
208_D	212_S	1.212	1.00
243_T	246_S	1.206	1.00
18_S	22_K	1.205	1.00
84_I	221_S	1.200	1.00
102_N	110_I	1.189	1.00
215_N	219_H	1.187	1.00
84_I	225_S	1.187	1.00
81_T	85_D	1.186	1.00
30_I	267_V	1.181	1.00
67_S	242_N	1.175	1.00
205_G	209_G	1.172	1.00
66_A	245_L	1.156	1.00
56_L	252_A	1.149	1.00
282_L	292_T	1.146	1.00
22_K	274_Q	1.140	1.00
230_D	234_R	1.131	1.00
8_I	24_D	1.129	1.00
116_Q	234_R	1.126	1.00
80_I	224_M	1.124	1.00
96_E	110_I	1.122	1.00
112_V	226_A	1.120	1.00
62_Y	245_L	1.120	1.00
51_K	106_E	1.113	1.00
207_L	211_D	1.109	1.00
50_L	53_N	1.108	1.00
126_N	136_N	1.105	1.00
64_S	68_Q	1.104	1.00
36_I	41_D	1.102	1.00
133_Y	136_N	1.098	1.00
61_Q	92_E	1.093	1.00
203_N	209_G	1.092	1.00
268_I	271_F	1.088	1.00
254_K	258_D	1.086	1.00
157_D	160_D	1.083	1.00
95_V	214_L	1.080	1.00
7_M	11_N	1.063	1.00
63_K	249_E	1.060	1.00
82_E	124_I	1.046	1.00
90_V	118_K	1.045	1.00
271_F	275_Q	1.044	1.00
28_S	37_S	1.043	1.00
70_F	242_N	1.042	1.00
66_A	238_L	1.041	1.00
264_L	268_I	1.034	1.00
84_I	88_S	1.023	1.00
283_A	287_Q	1.022	1.00
153_Y	156_S	1.017	1.00
195_L	198_F	1.009	1.00
83_G	124_I	1.001	1.00
28_S	32_S	0.986	1.00
60_Q	63_K	0.985	1.00
270_E	273_A	0.982	1.00
219_H	223_G	0.981	1.00
59_V	249_E	0.976	1.00
141_D	144_P	0.973	1.00
9_Q	13_L	0.970	1.00
141_D	146_T	0.970	1.00
23_L	27_Q	0.961	1.00
34_K	262_V	0.961	1.00
53_N	57_S	0.956	1.00
20_Y	278_H	0.955	1.00
11_N	14_R	0.951	1.00
88_S	221_S	0.943	1.00
19_S	23_L	0.942	1.00
27_Q	271_F	0.938	1.00
219_H	222_D	0.937	1.00
61_Q	65_N	0.934	1.00
13_L	17_G	0.934	1.00
114_V	118_K	0.925	1.00
120_Q	237_R	0.922	1.00
25_K	28_S	0.922	1.00
94_A	210_M	0.921	1.00
179_S	182_G	0.920	1.00
63_K	245_L	0.917	1.00
95_V	98_Q	0.915	1.00
59_V	63_K	0.914	1.00
49_S	256_L	0.913	1.00
14_R	250_E	0.912	1.00
84_I	224_M	0.912	1.00
115_K	199_E	0.906	1.00
99_N	102_N	0.905	1.00
95_V	218_D	0.905	1.00
26_L	262_V	0.905	1.00
70_F	74_E	0.904	1.00
94_A	198_F	0.902	1.00
71_T	129_V	0.893	1.00
70_F	235_Y	0.892	1.00
94_A	213_V	0.889	1.00
94_A	98_Q	0.888	1.00
212_S	216_D	0.886	1.00
161_V	164_N	0.884	1.00
91_R	214_L	0.880	1.00
160_D	163_V	0.879	1.00
278_H	282_L	0.872	1.00
184_T	189_Q	0.870	1.00
202_L	205_G	0.869	1.00
133_Y	144_P	0.860	1.00
267_V	270_E	0.859	1.00
114_V	202_L	0.858	1.00
199_E	202_L	0.856	1.00
125_A	135_F	0.853	1.00
89_K	117_L	0.852	1.00
190_N	193_E	0.851	1.00
13_L	269_T	0.851	1.00
215_N	218_D	0.850	1.00
199_E	203_N	0.850	1.00
93_L	96_E	0.850	1.00
19_S	22_K	0.848	1.00
94_A	217_I	0.848	1.00
82_E	240_L	0.847	1.00
270_E	274_Q	0.847	1.00
4_T	7_M	0.845	1.00
176_D	179_S	0.840	1.00
192_F	195_L	0.839	1.00
15_Y	32_S	0.839	1.00
4_T	34_K	0.838	1.00
159_A	164_N	0.834	1.00
16_I	278_H	0.830	1.00
85_D	88_S	0.826	1.00
74_E	235_Y	0.824	1.00
32_S	37_S	0.824	1.00
22_K	25_K	0.817	1.00
18_S	21_S	0.812	1.00
16_I	282_L	0.810	0.99
141_D	145_V	0.805	0.99
161_V	167_N	0.802	0.99
241_V	245_L	0.802	0.99
56_L	256_L	0.794	0.99
193_E	196_D	0.793	0.99
24_D	27_Q	0.792	0.99
226_A	230_D	0.791	0.99
157_D	161_V	0.789	0.99
291_P	295_D	0.789	0.99
222_D	226_A	0.787	0.99
98_Q	214_L	0.786	0.99
177_P	181_F	0.783	0.99
29_Q	262_V	0.783	0.99
81_T	228_R	0.778	0.99
275_Q	279_R	0.777	0.99
73_L	238_L	0.776	0.99
175_S	178_K	0.775	0.99
114_V	204_S	0.774	0.99
122_L	125_A	0.770	0.99
292_T	295_D	0.768	0.99
235_Y	239_E	0.764	0.99
138_T	157_D	0.763	0.99
69_A	238_L	0.763	0.99
283_A	295_D	0.760	0.99
63_K	67_S	0.757	0.99
210_M	213_V	0.751	0.99
142_K	146_T	0.750	0.99
111_G	118_K	0.750	0.99
47_M	109_A	0.747	0.99
88_S	92_E	0.745	0.99
196_D	200_K	0.740	0.99
118_K	196_D	0.736	0.99
19_S	274_Q	0.736	0.99
37_S	41_D	0.730	0.99
158_T	161_V	0.730	0.99
90_V	195_L	0.726	0.99
22_K	270_E	0.725	0.99
179_S	183_G	0.724	0.99
98_Q	210_M	0.721	0.99
248_Q	252_A	0.721	0.99
102_N	109_A	0.719	0.99
189_Q	193_E	0.718	0.99
98_Q	102_N	0.714	0.99
151_G	154_T	0.713	0.99
50_L	225_S	0.713	0.99
117_L	120_Q	0.713	0.99
23_L	275_Q	0.704	0.99
93_L	117_L	0.701	0.99
158_T	163_V	0.701	0.99
166_S	169_M	0.700	0.99
282_L	285_N	0.699	0.99
151_G	156_S	0.698	0.99
151_G	157_D	0.694	0.98
178_K	182_G	0.689	0.98
44_V	48_K	0.688	0.98
206_S	210_M	0.682	0.98
111_G	203_N	0.682	0.98
103_G	109_A	0.679	0.98
136_N	139_N	0.679	0.98
155_I	161_V	0.678	0.98
9_Q	269_T	0.675	0.98
111_G	205_G	0.675	0.98
242_N	246_S	0.674	0.98
16_I	281_T	0.671	0.98
139_N	157_D	0.671	0.98
57_S	60_Q	0.670	0.98
159_A	165_I	0.670	0.98
246_S	249_E	0.669	0.98
250_E	254_K	0.669	0.98
21_S	25_K	0.669	0.98
112_V	115_K	0.669	0.98
79_N	135_F	0.667	0.98
102_N	107_R	0.667	0.98
94_A	114_V	0.665	0.98
9_Q	12_S	0.661	0.98
107_R	210_M	0.659	0.98
19_S	278_H	0.658	0.98
77_E	231_L	0.657	0.98
79_N	127_T	0.656	0.98
213_V	217_I	0.655	0.98
76_T	175_S	0.654	0.98
271_F	278_H	0.652	0.98
66_A	241_V	0.652	0.98
221_S	225_S	0.652	0.98
113_E	233_A	0.651	0.98
97_A	107_R	0.647	0.98
23_L	26_L	0.646	0.98
216_D	219_H	0.646	0.98
223_G	226_A	0.644	0.97
279_R	283_A	0.643	0.97
273_A	277_V	0.642	0.97
175_S	181_F	0.642	0.97
60_Q	266_E	0.642	0.97
76_T	231_L	0.635	0.97
63_K	242_N	0.634	0.97
73_L	234_R	0.633	0.97
118_K	121_L	0.633	0.97
137_G	144_P	0.632	0.97
8_I	27_Q	0.631	0.97
137_G	141_D	0.631	0.97
150_D	155_I	0.630	0.97
103_G	131_G	0.630	0.97
21_S	24_D	0.629	0.97
165_I	241_V	0.628	0.97
209_G	213_V	0.626	0.97
139_N	144_P	0.625	0.97
281_T	284_V	0.623	0.97
153_Y	157_D	0.622	0.97
113_E	117_L	0.622	0.97
146_T	151_G	0.620	0.97
8_I	12_S	0.617	0.97
275_Q	287_Q	0.615	0.97
26_L	29_Q	0.615	0.97
239_E	243_T	0.609	0.96
175_S	180_A	0.607	0.96
192_F	197_S	0.607	0.96
191_V	195_L	0.604	0.96
136_N	142_K	0.601	0.96
55_Q	58_Q	0.599	0.96
7_M	10_Q	0.599	0.96
84_I	228_R	0.594	0.96
90_V	121_L	0.593	0.96
10_Q	14_R	0.593	0.96
112_V	229_S	0.591	0.96
213_V	216_D	0.591	0.96
280_A	283_A	0.590	0.96
71_T	74_E	0.589	0.96
81_T	84_I	0.589	0.96
97_A	210_M	0.588	0.96
158_T	178_K	0.587	0.96
212_S	215_N	0.585	0.96
35_K	64_S	0.583	0.96
78_T	251_T	0.582	0.95
23_L	278_H	0.580	0.95
247_A	251_T	0.579	0.95
247_A	250_E	0.578	0.95
57_S	88_S	0.577	0.95
83_G	135_F	0.577	0.95
47_M	130_N	0.577	0.95
15_Y	28_S	0.577	0.95
203_N	206_S	0.573	0.95
68_Q	71_T	0.572	0.95
133_Y	143_P	0.572	0.95
284_V	287_Q	0.569	0.95
160_D	165_I	0.569	0.95
116_Q	237_R	0.567	0.95
119_E	123_N	0.566	0.95
76_T	134_I	0.565	0.95
233_A	236_N	0.563	0.95
35_K	45_V	0.562	0.95
30_I	268_I	0.561	0.94
155_I	163_V	0.561	0.94
283_A	286_A	0.560	0.94
177_P	182_G	0.559	0.94
202_L	213_V	0.557	0.94
176_D	182_G	0.556	0.94
94_A	97_A	0.556	0.94
257_S	260_E	0.555	0.94
157_D	165_I	0.555	0.94
204_S	207_L	0.554	0.94
178_K	183_G	0.554	0.94
111_G	202_L	0.553	0.94
214_L	218_D	0.550	0.94
146_T	156_S	0.549	0.94
161_V	166_S	0.548	0.94
72_W	134_I	0.543	0.93
25_K	40_S	0.540	0.93
204_S	213_V	0.540	0.93
50_L	260_E	0.540	0.93
194_M	198_F	0.540	0.93
285_N	289_V	0.538	0.93
12_S	15_Y	0.538	0.93
161_V	168_N	0.536	0.93
125_A	192_F	0.536	0.93
278_H	281_T	0.534	0.93
96_E	102_N	0.534	0.93
264_L	293_L	0.533	0.93
150_D	154_T	0.532	0.93
79_N	83_G	0.531	0.93
8_I	16_I	0.529	0.93
80_I	228_R	0.528	0.93
229_S	237_R	0.527	0.92
128_Q	131_G	0.525	0.92
117_L	240_L	0.525	0.92
175_S	179_S	0.522	0.92
194_M	197_S	0.521	0.92
73_L	235_Y	0.521	0.92
165_I	168_N	0.521	0.92
196_D	199_E	0.520	0.92
163_V	167_N	0.519	0.92
25_K	38_K	0.518	0.92
143_P	154_T	0.516	0.92
17_G	20_Y	0.510	0.91
244_R	248_Q	0.510	0.91
151_G	155_I	0.509	0.91
111_G	204_S	0.509	0.91
151_G	160_D	0.508	0.91
54_T	131_G	0.508	0.91
136_N	144_P	0.506	0.91
46_A	50_L	0.505	0.91
151_G	161_V	0.504	0.91
164_N	168_N	0.503	0.91
75_N	129_V	0.503	0.91
208_D	211_D	0.503	0.91
158_T	162_V	0.502	0.91
36_I	260_E	0.502	0.91
271_F	287_Q	0.500	0.90

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1ucuA	1	1	100	0.262	Contact Map
3pwxA	2	0.7349	100	0.367	Contact Map
2d4xA	1	0.6477	100	0.39	Contact Map
3k8wA	1	0.7181	100	0.45	Contact Map
4nx9A	1	0.6409	100	0.57	Contact Map
3v47C	1	0.5604	99.9	0.611	Contact Map
4cfiA	1	0.5705	99.9	0.658	Contact Map
1io1A	1	0.6711	99.8	0.666	Contact Map
3k1iC	1	0.3993	99.4	0.775	Contact Map
1oryB	2	0.1275	97.3	0.878	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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