GREMLIN Database
MSCL - Large-conductance mechanosensitive channel
UniProt: P94585 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 130 (121)
Sequences: 2842 (1820)
Seq/√Len: 165.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
32_T36_N3.6191.00
3_N7_A3.4091.00
110_A113_E3.0991.00
4_E88_R2.8951.00
29_K32_T2.2651.00
110_A116_K2.1551.00
56_S63_V2.1071.00
4_E91_N2.1031.00
28_G32_T2.0341.00
33_S37_D1.9951.00
65_K68_S1.9541.00
81_F85_I1.9511.00
15_V90_L1.9241.00
55_L66_Y1.9021.00
37_D68_S1.8791.00
109_D112_E1.7631.00
34_L38_I1.7331.00
34_L69_F1.7071.00
111_Q114_L1.6961.00
94_R97_K1.6701.00
17_L21_V1.6321.00
107_A113_E1.6121.00
21_V28_G1.6041.00
56_S61_D1.6011.00
38_I64_V1.5581.00
75_N79_I1.5241.00
7_A95_R1.5031.00
108_V113_E1.4821.00
29_K75_N1.4641.00
9_A80_S1.4581.00
17_L20_G1.4551.00
109_D113_E1.4431.00
71_Q75_N1.4321.00
113_E116_K1.4181.00
56_S65_K1.3411.00
68_S72_T1.3331.00
42_L64_V1.3091.00
56_S60_G1.2701.00
108_V116_K1.2441.00
115_L119_R1.2421.00
57_F64_V1.2321.00
58_T63_V1.2251.00
4_E84_F1.2091.00
117_E123_K1.2061.00
44_G52_F1.2011.00
85_I89_T1.1961.00
9_A77_L1.1751.00
25_G29_K1.1561.00
121_L124_Q1.1521.00
52_F70_I1.1411.00
116_K121_L1.1321.00
94_R98_E1.1301.00
57_F66_Y1.1091.00
30_I73_I1.0901.00
32_T35_V1.0851.00
108_V112_E1.0670.99
96_K99_A1.0510.99
117_E120_D1.0410.99
37_D69_F1.0390.99
110_A117_E1.0340.99
112_E116_K1.0320.99
5_F81_F1.0300.99
58_T61_D1.0250.99
14_I34_L1.0190.99
11_R94_R1.0030.99
92_G96_K0.9850.99
45_L59_F0.9810.99
15_V86_V0.9660.99
22_V86_V0.9660.99
5_F80_S0.9650.99
32_T75_N0.9530.99
83_I86_V0.9410.99
95_R99_A0.9280.99
108_V117_E0.9280.99
14_I21_V0.9240.98
4_E89_T0.9240.98
89_T93_L0.9130.98
52_F74_V0.9110.98
38_I69_F0.8970.98
37_D72_T0.8950.98
99_A102_E0.8910.98
2_W77_L0.8850.98
33_S68_S0.8700.98
58_T62_A0.8670.98
40_M44_G0.8560.98
109_D114_L0.8550.97
95_R98_E0.8400.97
37_D65_K0.8330.97
91_N94_R0.8330.97
13_N16_D0.8310.97
112_E121_L0.8220.97
42_L46_L0.8020.96
22_V82_S0.8000.96
111_Q116_K0.7990.96
14_I30_I0.7910.96
51_D54_G0.7880.96
44_G62_A0.7830.96
7_A91_N0.7820.96
111_Q121_L0.7820.96
10_M14_I0.7810.96
40_M52_F0.7760.96
40_M43_V0.7610.95
47_L55_L0.7580.95
114_L119_R0.7580.95
22_V35_V0.7580.95
19_I87_I0.7570.95
21_V25_G0.7510.95
53_S71_Q0.7500.95
71_Q79_I0.7300.94
49_G55_L0.7280.94
89_T92_G0.7220.94
56_S62_A0.7220.94
105_E108_V0.7220.94
92_G95_R0.7190.93
78_I81_F0.7140.93
52_F71_Q0.7090.93
36_N40_M0.7060.93
28_G82_S0.7050.93
56_S59_F0.6980.92
23_I83_I0.6980.92
28_G79_I0.6960.92
109_D116_K0.6890.92
39_I82_S0.6840.92
39_I78_I0.6780.91
44_G67_G0.6780.91
40_M71_Q0.6740.91
99_A103_A0.6720.91
42_L47_L0.6530.90
16_D20_G0.6500.89
33_S69_F0.6460.89
44_G47_L0.6450.89
108_V111_Q0.6400.89
61_D65_K0.6390.89
110_A121_L0.6320.88
35_V40_M0.6240.87
69_F73_I0.6190.87
24_G90_L0.6180.87
47_L50_L0.6150.87
104_A107_A0.6100.86
56_S66_Y0.6090.86
11_R16_D0.6050.86
77_L81_F0.6030.86
9_A20_G0.5960.85
93_L97_K0.5950.85
87_I91_N0.5940.85
20_G91_N0.5940.85
45_L62_A0.5890.84
70_I74_V0.5890.84
19_I83_I0.5890.84
15_V19_I0.5820.84
43_V79_I0.5820.84
28_G31_V0.5790.83
3_N6_K0.5770.83
51_D71_Q0.5760.83
81_F89_T0.5690.82
74_V78_I0.5690.82
5_F9_A0.5670.82
97_K102_E0.5660.82
57_F62_A0.5650.82
24_G28_G0.5610.81
113_E121_L0.5610.81
46_L49_G0.5590.81
102_E105_E0.5560.81
45_L49_G0.5530.80
5_F8_F0.5500.80
90_L94_R0.5440.79
16_D19_I0.5400.79
98_E102_E0.5370.78
22_V79_I0.5370.78
66_Y70_I0.5260.77
86_V89_T0.5250.77
44_G50_L0.5230.77
44_G49_G0.5170.76
55_L70_I0.5170.76
16_D24_G0.5160.76
14_I19_I0.5120.75
119_R123_K0.5110.75
106_E109_D0.5100.75
16_D26_A0.5090.75
42_L59_F0.5010.74
46_L82_S0.5010.74
45_L64_V0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2oarA 7 0.9154 100 0.117 Contact Map
3hzqA 3 0.7077 100 0.285 Contact Map
4lkuA 3 0.2231 95.8 0.863 Contact Map
2riqA 2 0.2692 41.1 0.926 Contact Map
4rfsS 1 0.6385 24 0.934 Contact Map
2j69A 1 0.7385 21.8 0.936 Contact Map
2kluA 1 0.4462 21.4 0.936 Contact Map
3rlbA 1 0.6462 16.4 0.939 Contact Map
2mi2A 1 0.5 16.3 0.939 Contact Map
2yfvC 1 0.1769 14.7 0.94 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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