GREMLIN Database
YSAA - Putative uncharacterized hydrolase YsaA
UniProt: P94512 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 260 (209)
Sequences: 1038 (863)
Seq/√Len: 59.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
208_I229_V3.6571.00
135_E233_R3.6311.00
197_L207_A3.5021.00
221_A228_T3.4811.00
144_K175_N3.1201.00
161_K182_A3.0181.00
160_Q163_K3.0161.00
249_S253_D2.6641.00
148_E175_N2.6551.00
197_L226_I2.6151.00
133_Y233_R2.5211.00
211_G217_D2.4401.00
202_I207_A2.4261.00
203_E206_D2.3421.00
207_A226_I2.2891.00
150_L176_E2.2551.00
118_L122_F2.2231.00
193_F220_G2.2111.00
248_I254_L2.1611.00
122_F126_R2.0831.00
192_I196_C1.8991.00
229_V254_L1.8481.00
53_Y56_Y1.8411.00
155_G160_Q1.7460.99
190_V194_E1.7450.99
178_V196_C1.7290.99
140_L149_L1.7100.99
154_N217_D1.6730.99
135_E138_A1.6470.99
254_L258_L1.6270.99
150_L200_M1.6210.99
133_Y136_T1.6090.99
48_A51_E1.5640.99
135_E139_V1.5610.99
152_L192_I1.5440.98
163_K212_D1.5430.98
135_E251_L1.5150.98
62_T66_G1.5140.98
164_L211_G1.5050.98
153_T160_Q1.5030.98
155_G159_L1.4760.98
248_I253_D1.4720.98
150_L196_C1.4670.98
180_S211_G1.4410.97
141_D173_Y1.4270.97
71_E74_W1.4250.97
222_S245_D1.4200.97
160_Q186_G1.4190.97
138_A141_D1.3940.97
115_G122_F1.3930.97
152_L209_M1.3930.97
195_H199_L1.3660.97
91_I94_E1.3380.96
218_I228_T1.3260.96
157_P182_A1.3100.96
94_E98_N1.3080.96
178_V195_H1.3010.95
92_V96_R1.2960.95
155_G163_K1.2960.95
160_Q179_I1.2910.95
98_N102_N1.2810.95
219_L223_R1.2780.95
250_S255_F1.2690.95
227_K245_D1.2660.95
174_F177_I1.2640.95
157_P181_G1.2490.94
246_Y257_I1.2470.94
149_L174_F1.2450.94
119_G126_R1.2370.94
210_V229_V1.2360.94
51_E54_M1.2350.94
178_V192_I1.2290.94
180_S192_I1.2190.93
232_N247_I1.2070.93
183_F192_I1.1920.93
154_N188_P1.1700.92
135_E232_N1.1640.92
193_F221_A1.1550.91
145_G203_E1.1480.91
221_A226_I1.1420.91
53_Y57_E1.1340.91
194_E198_K1.1270.90
137_F141_D1.1000.89
231_I234_T1.0900.89
208_I227_K1.0890.89
132_V137_F1.0870.89
153_T163_K1.0820.89
156_D160_Q1.0800.88
97_R116_E1.0790.88
176_E199_L1.0780.88
151_L179_I1.0770.88
221_A224_A1.0720.88
85_F88_L1.0700.88
153_T186_G1.0650.88
185_K191_S1.0410.87
119_G123_A1.0400.87
226_I233_R1.0390.86
154_N230_W1.0340.86
140_L174_F1.0320.86
231_I248_I1.0300.86
155_G179_I1.0240.86
135_E250_S1.0160.85
180_S220_G1.0140.85
52_L55_S1.0130.85
66_G70_F1.0060.85
155_G193_F0.9970.84
185_K189_D0.9970.84
138_A252_H0.9930.84
96_R163_K0.9860.83
61_Y196_C0.9860.83
222_S228_T0.9820.83
154_N211_G0.9760.83
160_Q230_W0.9740.83
213_N220_G0.9720.83
151_L174_F0.9700.82
155_G230_W0.9700.82
193_F209_M0.9680.82
115_G119_G0.9650.82
202_I206_D0.9630.82
183_F195_H0.9610.82
209_M221_A0.9590.82
49_A53_Y0.9530.81
112_P116_E0.9480.81
142_Q145_G0.9440.81
154_N210_V0.9340.80
74_W78_S0.9270.79
72_G76_N0.9250.79
117_Y124_A0.9190.79
77_F81_I0.9190.79
157_P161_K0.9190.79
114_Y118_L0.9160.79
70_F74_W0.9130.78
139_V255_F0.9120.78
218_I230_W0.9100.78
71_E75_S0.9090.78
178_V199_L0.9060.78
186_G211_G0.9030.78
190_V223_R0.9020.78
132_V170_L0.8980.77
164_L174_F0.8960.77
195_H198_K0.8950.77
187_K217_D0.8920.77
101_T115_G0.8740.75
142_Q255_F0.8730.75
96_R158_S0.8720.75
140_L154_N0.8720.75
88_L99_A0.8700.75
92_V100_W0.8700.75
84_G87_K0.8680.75
113_A201_N0.8660.75
179_I220_G0.8640.74
139_V210_V0.8640.74
179_I183_F0.8550.74
66_G69_P0.8550.74
159_L209_M0.8460.73
118_L123_A0.8390.72
213_N216_T0.8330.72
160_Q209_M0.8260.71
248_I255_F0.8190.70
232_N235_D0.8100.70
96_R160_Q0.8080.69
147_Y258_L0.8070.69
165_A171_A0.8060.69
190_V198_K0.8030.69
112_P194_E0.8020.69
155_G181_G0.8000.69
96_R100_W0.7950.68
140_L170_L0.7830.67
71_E82_S0.7820.67
100_W122_F0.7760.66
163_K230_W0.7760.66
130_P153_T0.7740.66
73_L77_F0.7740.66
240_T243_K0.7700.66
128_K132_V0.7670.65
105_K110_D0.7630.65
155_G209_M0.7620.65
218_I229_V0.7580.64
49_A52_L0.7570.64
133_Y232_N0.7540.64
209_M217_D0.7490.64
136_T251_L0.7480.63
190_V196_C0.7480.63
181_G186_G0.7430.63
84_G89_N0.7420.63
115_G118_L0.7420.63
52_L57_E0.7390.63
94_E97_R0.7390.63
78_S82_S0.7360.62
190_V224_A0.7350.62
153_T212_D0.7300.62
154_N186_G0.7290.61
208_I245_D0.7280.61
130_P155_G0.7270.61
50_R54_M0.7240.61
252_H255_F0.7240.61
152_L211_G0.7190.60
51_E199_L0.7170.60
67_I71_E0.7070.59
93_P143_L0.7060.59
112_P115_G0.7040.59
88_L226_I0.7030.59
153_T222_S0.6920.57
217_D220_G0.6900.57
68_N229_V0.6900.57
96_R212_D0.6880.57
135_E152_L0.6870.57
102_N105_K0.6850.57
75_S214_L0.6850.57
164_L212_D0.6810.56
165_A234_T0.6790.56
54_M63_V0.6790.56
218_I246_Y0.6770.56
108_G145_G0.6760.56
90_K94_E0.6760.56
209_M216_T0.6760.56
211_G220_G0.6750.56
142_Q258_L0.6690.55
69_P72_G0.6690.55
154_N220_G0.6650.54
139_V143_L0.6620.54
196_C199_L0.6610.54
131_F134_D0.6590.54
243_K247_I0.6590.54
65_I68_N0.6580.54
136_T175_N0.6540.53
100_W126_R0.6540.53
48_A53_Y0.6490.53
186_G212_D0.6480.53
171_A174_F0.6430.52
165_A253_D0.6420.52
141_D198_K0.6420.52
113_A141_D0.6390.52
149_L206_D0.6390.52
212_D230_W0.6390.52
136_T220_G0.6310.51
241_D252_H0.6310.51
156_D212_D0.6300.51
197_L204_K0.6280.50
177_I207_A0.6170.49
154_N180_S0.6160.49
114_Y124_A0.6160.49
138_A250_S0.6150.49
49_A57_E0.6150.49
130_P156_D0.6130.49
119_G124_A0.6110.48
162_E166_G0.6110.48
51_E55_S0.6080.48
238_N242_V0.6050.48
159_L174_F0.6010.47
233_R236_K0.6010.47
247_I253_D0.5990.47
130_P139_V0.5980.47
143_L210_V0.5970.47
153_T234_T0.5950.47
97_R112_P0.5930.46
125_E128_K0.5920.46
121_F125_E0.5920.46
185_K192_I0.5910.46
139_V233_R0.5880.46
82_S85_F0.5860.46
227_K246_Y0.5860.46
153_T218_I0.5850.46
122_F127_R0.5840.45
131_F139_V0.5790.45
54_M84_G0.5780.45
156_D179_I0.5780.45
100_W159_L0.5740.44
135_E231_I0.5720.44
153_T179_I0.5650.43
100_W153_T0.5620.43
66_G75_S0.5610.43
181_G216_T0.5590.43
160_Q164_L0.5580.43
165_A182_A0.5580.43
80_P84_G0.5540.42
98_N101_T0.5540.42
67_I75_S0.5510.42
182_A252_H0.5510.42
142_Q146_K0.5500.42
181_G213_N0.5480.41
165_A252_H0.5470.41
133_Y213_N0.5460.41
76_N106_A0.5450.41
156_D163_K0.5440.41
249_S252_H0.5430.41
159_L163_K0.5400.41
149_L175_N0.5380.40
156_D209_M0.5350.40
105_K190_V0.5340.40
115_G204_K0.5320.40
163_K179_I0.5310.40
153_T229_V0.5300.40
76_N80_P0.5300.40
80_P105_K0.5280.39
201_N243_K0.5280.39
182_A201_N0.5270.39
117_Y223_R0.5240.39
188_P217_D0.5230.39
214_L219_L0.5230.39
90_K121_F0.5230.39
220_G224_A0.5210.39
249_S256_P0.5180.38
75_S82_S0.5140.38
208_I220_G0.5100.37
70_F73_L0.5060.37
209_M213_N0.5060.37
143_L254_L0.5060.37
121_F124_A0.5050.37
120_E123_A0.5040.37
94_E171_A0.5030.37
186_G233_R0.5030.37
176_E200_M0.5020.37
48_A52_L0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3qnmA 1 0.8808 100 0.39 Contact Map
3ed5A 1 0.8654 100 0.395 Contact Map
3k1zA 1 0.8885 100 0.416 Contact Map
3umgA 2 0.8731 100 0.42 Contact Map
3u26A 1 0.8731 100 0.421 Contact Map
4uw9A 1 0.8154 100 0.422 Contact Map
3smvA 1 0.8462 100 0.422 Contact Map
2om6A 2 0.8615 100 0.423 Contact Map
3umcA 2 0.8615 100 0.426 Contact Map
3vayA 2 0.8615 100 0.427 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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