GREMLIN Database
HFLX - GTPase HflX
UniProt: P94478 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 420 (394)
Sequences: 3536 (2059)
Seq/√Len: 103.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
66_V98_A3.8691.00
37_T82_I3.6021.00
61_I69_L3.5511.00
73_V101_V3.5251.00
133_Q178_N3.5071.00
284_L318_A3.3321.00
66_V95_L2.9811.00
204_A280_A2.9021.00
52_R56_D2.9001.00
34_A47_S2.8671.00
239_T251_L2.7271.00
31_E47_S2.6881.00
287_I317_T2.6691.00
241_K249_S2.6541.00
65_K68_E2.6091.00
35_S38_K2.5691.00
246_S356_Q2.5611.00
81_L99_I2.4341.00
48_V69_L2.4121.00
73_V81_L2.3631.00
354_A357_R2.3391.00
368_E401_D2.3361.00
128_E173_R2.2821.00
70_K74_E2.2381.00
37_T42_G2.2361.00
85_D261_D2.2281.00
46_T72_L2.2211.00
203_I250_V2.1911.00
302_L315_M2.1831.00
219_R348_A2.1731.00
366_S401_D2.1541.00
48_V72_L2.1481.00
125_L177_I2.0921.00
239_T253_S2.0921.00
17_Q51_K2.0891.00
12_I78_A2.0561.00
39_T117_R2.0551.00
215_T342_A2.0431.00
394_N399_Q2.0331.00
368_E399_Q2.0251.00
318_A351_F2.0061.00
95_L99_I2.0001.00
268_A271_R1.9431.00
291_N297_H1.9421.00
14_V83_F1.9161.00
316_L354_A1.9111.00
14_V61_I1.8851.00
306_E311_D1.8841.00
354_A358_Y1.8771.00
390_R401_D1.8651.00
298_E317_T1.8371.00
381_R385_E1.8121.00
165_T169_H1.7591.00
11_A42_G1.7521.00
366_S403_S1.7491.00
182_S185_I1.7321.00
284_L351_F1.7311.00
322_R339_M1.7281.00
226_Y230_L1.7241.00
206_V283_I1.7211.00
112_D131_Q1.7071.00
81_L101_V1.7021.00
264_T304_L1.6981.00
185_I275_E1.6911.00
70_K98_A1.6781.00
318_A338_I1.6681.00
299_K303_R1.6671.00
185_I271_R1.6581.00
282_L355_I1.6531.00
289_S319_Y1.6511.00
143_G147_S1.6211.00
293_D296_G1.6161.00
122_E188_R1.6091.00
8_Q43_K1.5981.00
39_T230_L1.5961.00
143_G146_L1.5871.00
61_I83_F1.5781.00
383_K391_F1.5731.00
176_E180_Q1.5711.00
370_H399_Q1.5711.00
143_G162_K1.5641.00
196_K279_E1.5621.00
188_R275_E1.5621.00
150_G155_A1.5561.00
370_H397_N1.5541.00
287_I298_E1.5491.00
11_A80_L1.5491.00
214_S225_S1.5451.00
218_N227_E1.5261.00
278_K309_E1.5031.00
290_S324_Q1.4931.00
219_R345_E1.4911.00
73_V99_I1.4821.00
303_R307_E1.4771.00
225_S238_M1.4751.00
12_I72_L1.4641.00
121_R180_Q1.4531.00
145_N162_K1.4501.00
330_I334_G1.4491.00
67_E71_A1.4431.00
128_E132_L1.4391.00
379_L391_F1.4261.00
174_I178_N1.4221.00
60_Y85_D1.4201.00
316_L358_Y1.4121.00
285_H301_V1.4071.00
344_F347_D1.3941.00
56_D63_K1.3901.00
66_V99_I1.3761.00
319_Y339_M1.3721.00
149_Q155_A1.3661.00
26_F49_T1.3600.99
15_G30_M1.3520.99
387_M403_S1.3490.99
215_T340_V1.3340.99
187_H190_R1.3250.99
302_L317_T1.3190.99
185_I272_S1.3160.99
221_T242_M1.3070.99
226_Y233_A1.3060.99
330_I333_A1.2980.99
192_R275_E1.2910.99
175_H179_T1.2900.99
9_E102_K1.2880.99
347_D350_A1.2850.99
129_L174_I1.2850.99
379_L400_Y1.2840.99
353_E357_R1.2730.99
373_A397_N1.2730.99
37_T109_L1.2690.99
298_E302_L1.2680.99
31_E34_A1.2590.99
59_T66_V1.2570.99
143_G150_G1.2540.99
382_I400_Y1.2510.99
242_M252_L1.2510.99
67_E98_A1.2420.99
72_L76_L1.2400.99
125_L184_V1.2400.99
265_T307_E1.2380.99
205_L217_F1.2340.99
45_L76_L1.2300.99
243_V249_S1.2220.99
282_L316_L1.2160.99
326_L329_F1.2080.99
12_I46_T1.2060.99
389_D403_S1.2020.99
10_K43_K1.1860.99
42_G82_I1.1850.99
153_I158_P1.1840.99
39_T259_I1.1830.99
336_D354_A1.1820.99
71_A74_E1.1730.98
203_I284_L1.1700.98
17_Q23_D1.1700.98
372_P375_E1.1690.98
289_S294_Y1.1680.98
33_L82_I1.1680.98
285_H298_E1.1680.98
318_A340_V1.1510.98
133_Q265_T1.1510.98
206_V305_L1.1510.98
294_Y319_Y1.1480.98
42_G153_I1.1430.98
287_I294_Y1.1420.98
73_V78_A1.1390.98
42_G79_D1.1340.98
202_Q251_L1.1280.98
46_T78_A1.1230.98
46_T76_L1.1180.98
141_G155_A1.1150.98
242_M250_V1.1010.98
71_A75_E1.0990.98
291_N294_Y1.0980.98
295_A299_K1.0970.98
244_L250_V1.0940.98
190_R193_E1.0920.98
369_A402_I1.0910.98
136_L171_R1.0890.98
316_L355_I1.0800.97
210_N229_D1.0690.97
296_G299_K1.0680.97
149_Q162_K1.0670.97
203_I282_L1.0660.97
141_G144_I1.0630.97
9_E42_G1.0600.97
142_Q145_N1.0490.97
371_V378_L1.0460.97
39_T231_L1.0450.97
141_G150_G1.0410.97
131_Q163_L1.0410.97
94_S97_T1.0390.97
40_A112_D1.0370.97
55_A60_Y1.0340.97
187_H191_Y1.0340.97
42_G112_D1.0260.96
115_A158_P1.0260.96
185_I268_A1.0230.96
131_Q158_P1.0210.96
115_A131_Q1.0200.96
298_E319_Y1.0190.96
16_C50_Q1.0190.96
15_G29_S1.0190.96
375_E378_L1.0150.96
117_R226_Y1.0150.96
310_A313_I1.0120.96
131_Q135_A1.0110.96
264_T267_I1.0100.96
351_F355_I1.0050.96
45_L78_A0.9980.96
141_G145_N0.9960.96
42_G80_L0.9940.96
373_A393_F0.9930.96
393_F398_E0.9910.96
366_S389_D0.9900.96
61_I66_V0.9870.96
245_P356_Q0.9850.96
285_H291_N0.9830.95
80_L102_K0.9790.95
285_H315_M0.9780.95
147_S150_G0.9750.95
74_E100_E0.9700.95
70_K100_E0.9690.95
149_Q161_T0.9640.95
332_T335_R0.9640.95
112_D156_R0.9630.95
160_E168_R0.9620.95
189_S196_K0.9580.95
345_E348_A0.9560.95
34_A38_K0.9560.95
193_E197_K0.9500.95
37_T44_V0.9490.95
122_E184_V0.9490.95
338_I354_A0.9460.94
72_L78_A0.9440.94
16_C55_A0.9420.94
370_H394_N0.9420.94
11_A82_I0.9410.94
156_R163_L0.9280.94
370_H373_A0.9260.94
133_Q174_I0.9250.94
229_D324_Q0.9250.94
336_D358_Y0.9220.94
141_G162_K0.9220.94
380_S384_S0.9210.94
162_K169_H0.9140.93
13_L33_L0.9090.93
123_G158_P0.9090.93
27_E31_E0.9080.93
14_V69_L0.9060.93
12_I69_L0.8990.93
386_T404_G0.8970.93
371_V398_E0.8960.93
183_T186_R0.8930.93
10_K77_E0.8920.93
290_S325_K0.8900.92
28_N293_D0.8890.92
13_L34_A0.8890.92
179_T182_S0.8870.92
97_T100_E0.8860.92
271_R307_E0.8760.92
84_N261_D0.8750.92
217_F300_T0.8710.92
175_H178_N0.8650.91
317_T337_H0.8610.91
217_F252_L0.8590.91
194_R197_K0.8570.91
277_V283_I0.8540.91
294_Y298_E0.8520.91
145_N169_H0.8520.91
63_K67_E0.8520.91
389_D401_D0.8510.91
29_S261_D0.8440.90
69_L99_I0.8430.90
164_E168_R0.8420.90
27_E47_S0.8380.90
228_E233_A0.8360.90
143_G169_H0.8330.90
206_V277_V0.8320.90
394_N397_N0.8320.90
40_A131_Q0.8310.90
340_V348_A0.8290.89
373_A394_N0.8270.89
186_R190_R0.8260.89
120_T123_G0.8260.89
371_V375_E0.8250.89
39_T226_Y0.8250.89
350_A353_E0.8240.89
86_E265_T0.8210.89
382_I402_I0.8190.89
392_Y399_Q0.8180.89
200_V251_L0.8180.89
132_L170_I0.8170.89
172_N175_H0.8140.89
40_A115_A0.8140.89
205_L216_W0.8120.89
48_V78_A0.8110.88
41_D116_K0.8090.88
170_I174_I0.8040.88
123_G237_P0.8020.88
143_G158_P0.7960.88
70_K99_I0.7950.87
188_R269_A0.7920.87
230_L233_A0.7910.87
220_L352_K0.7900.87
42_G131_Q0.7880.87
160_E164_E0.7870.87
382_I386_T0.7840.87
277_V280_A0.7830.87
39_T112_D0.7830.87
39_T228_E0.7830.87
386_T402_I0.7830.87
248_Y360_R0.7810.87
331_P334_G0.7780.86
102_K112_D0.7760.86
386_T391_F0.7760.86
358_Y361_Q0.7700.86
122_E269_A0.7700.86
214_S227_E0.7670.86
102_K131_Q0.7660.85
30_M47_S0.7620.85
59_T63_K0.7610.85
38_K44_V0.7610.85
327_P331_P0.7580.85
129_L177_I0.7570.85
10_K45_L0.7550.85
140_T143_G0.7520.84
50_Q65_K0.7510.84
181_L184_V0.7460.84
147_S153_I0.7410.84
304_L308_L0.7390.83
245_P352_K0.7380.83
146_L166_D0.7350.83
96_A152_G0.7330.83
172_N176_E0.7330.83
123_G269_A0.7300.83
155_A165_T0.7290.83
82_I109_L0.7270.82
379_L398_E0.7260.82
147_S156_R0.7240.82
13_L47_S0.7220.82
349_A352_K0.7220.82
115_A156_R0.7200.82
147_S155_A0.7180.82
30_M33_L0.7160.81
224_D228_E0.7150.81
35_S39_T0.7150.81
120_T124_K0.7120.81
104_I109_L0.7120.81
92_L108_Q0.7110.81
201_L359_L0.7100.81
329_F333_A0.7090.81
383_K388_V0.7090.81
287_I319_Y0.7080.81
300_T304_L0.7060.80
67_E70_K0.7060.80
35_S260_Q0.7060.80
182_S186_R0.7030.80
147_S162_K0.7020.80
236_D301_V0.7010.80
394_N398_E0.6990.80
242_M355_I0.6980.80
324_Q343_K0.6930.79
70_K73_V0.6910.79
299_K302_L0.6910.79
169_H172_N0.6900.79
139_L167_R0.6900.79
15_G26_F0.6900.79
59_T98_A0.6880.79
34_A44_V0.6880.79
390_R399_Q0.6850.78
203_I355_I0.6850.78
40_A116_K0.6840.78
39_T205_L0.6810.78
210_N228_E0.6730.77
30_M49_T0.6720.77
149_Q156_R0.6710.77
221_T250_V0.6710.77
180_Q268_A0.6700.77
344_F349_A0.6690.77
300_T303_R0.6680.77
349_A353_E0.6660.76
214_S252_L0.6630.76
237_P252_L0.6620.76
289_S325_K0.6610.76
42_G102_K0.6600.76
112_D115_A0.6570.76
96_A102_K0.6550.75
15_G261_D0.6550.75
162_K166_D0.6540.75
154_G158_P0.6540.75
223_A237_P0.6490.75
131_Q153_I0.6480.75
247_G356_Q0.6480.75
40_A153_I0.6470.74
370_H396_E0.6460.74
192_R276_E0.6460.74
369_A378_L0.6440.74
367_F402_I0.6430.74
31_E324_Q0.6430.74
115_A155_A0.6410.74
236_D266_L0.6380.73
123_G218_N0.6380.73
334_G357_R0.6370.73
122_E275_E0.6360.73
373_A398_E0.6340.73
28_N291_N0.6330.73
144_I150_G0.6310.73
43_K77_E0.6300.72
141_G146_L0.6300.72
43_K47_S0.6290.72
17_Q26_F0.6290.72
149_Q153_I0.6270.72
147_S158_P0.6270.72
329_F334_G0.6270.72
163_L166_D0.6260.72
353_E356_Q0.6250.72
77_E183_T0.6230.72
88_S91_Q0.6230.72
144_I147_S0.6230.72
13_L16_C0.6210.71
363_L403_S0.6210.71
178_N268_A0.6200.71
146_L162_K0.6190.71
275_E279_E0.6180.71
141_G147_S0.6170.71
319_Y337_H0.6170.71
131_Q165_T0.6170.71
147_S169_H0.6160.71
280_A310_A0.6140.71
86_E107_T0.6140.71
9_E131_Q0.6130.70
50_Q62_G0.6120.70
60_Y83_F0.6120.70
141_G166_D0.6100.70
248_Y356_Q0.6100.70
248_Y363_L0.6090.70
328_D331_P0.6080.70
23_D27_E0.6070.70
219_R343_K0.6050.69
143_G148_R0.6050.69
376_G380_S0.6030.69
137_P217_F0.6030.69
304_L307_E0.6030.69
112_D153_I0.6020.69
288_D321_K0.6020.69
303_R306_E0.6000.69
383_K386_T0.6000.69
194_R198_N0.6000.69
376_G398_E0.5980.69
388_V391_F0.5970.68
329_F332_T0.5970.68
326_L331_P0.5960.68
87_L91_Q0.5950.68
35_S268_A0.5940.68
29_S260_Q0.5930.68
93_K96_A0.5920.68
62_G65_K0.5900.67
238_M383_K0.5900.67
29_S35_S0.5890.67
391_F397_N0.5890.67
158_P301_V0.5880.67
29_S33_L0.5870.67
379_L383_K0.5870.67
205_L286_L0.5870.67
131_Q156_R0.5860.67
69_L73_V0.5860.67
129_L301_V0.5850.67
302_L306_E0.5840.67
97_T101_V0.5840.67
90_S93_K0.5830.67
287_I302_L0.5830.67
203_I358_Y0.5800.66
191_Y268_A0.5790.66
150_G191_Y0.5770.66
108_Q137_P0.5760.66
286_L320_N0.5760.66
331_P335_R0.5750.66
161_T165_T0.5730.65
26_F51_K0.5730.65
145_N149_Q0.5720.65
222_S243_V0.5700.65
121_R124_K0.5700.65
102_K190_R0.5690.65
389_D392_Y0.5660.64
327_P335_R0.5660.64
87_L280_A0.5660.64
33_L83_F0.5650.64
248_Y359_L0.5650.64
214_S226_Y0.5640.64
29_S52_R0.5620.64
92_L145_N0.5620.64
178_N182_S0.5620.64
111_L218_N0.5610.64
367_F400_Y0.5600.63
334_G339_M0.5600.63
153_I156_R0.5590.63
185_I189_S0.5580.63
217_F227_E0.5570.63
25_H296_G0.5570.63
325_K329_F0.5560.63
227_E230_L0.5560.63
221_T224_D0.5550.63
135_A142_Q0.5530.63
217_F239_T0.5530.63
108_Q142_Q0.5500.62
188_R201_L0.5500.62
175_H183_T0.5500.62
26_F47_S0.5500.62
189_S361_Q0.5490.62
79_D272_S0.5480.62
162_K218_N0.5470.62
104_I108_Q0.5460.62
139_L163_L0.5450.61
12_I48_V0.5450.61
348_A352_K0.5440.61
207_G257_G0.5430.61
15_G18_L0.5420.61
303_R331_P0.5410.61
35_S229_D0.5410.61
9_E79_D0.5400.61
291_N295_A0.5400.61
33_L44_V0.5390.61
144_I162_K0.5390.61
264_T307_E0.5390.61
208_Y263_P0.5380.60
211_A269_A0.5380.60
182_S357_R0.5380.60
298_E301_V0.5370.60
193_E196_K0.5370.60
152_G160_E0.5360.60
309_E313_I0.5360.60
371_V379_L0.5350.60
8_Q38_K0.5350.60
340_V343_K0.5340.60
383_K393_F0.5340.60
143_G155_A0.5340.60
340_V344_F0.5330.60
42_G155_A0.5330.60
48_V76_L0.5320.60
206_V310_A0.5320.60
202_Q308_L0.5310.59
369_A393_F0.5290.59
159_G168_R0.5290.59
392_Y398_E0.5280.59
19_P53_N0.5270.59
121_R125_L0.5270.59
12_I81_L0.5270.59
59_T94_S0.5260.59
216_W340_V0.5250.59
279_E343_K0.5240.58
60_Y191_Y0.5240.58
377_K398_E0.5220.58
148_R192_R0.5220.58
250_V355_I0.5220.58
233_A266_L0.5220.58
16_C61_I0.5210.58
369_A372_P0.5210.58
63_K98_A0.5210.58
25_H28_N0.5210.58
350_A354_A0.5200.58
370_H374_S0.5200.58
62_G87_L0.5200.58
39_T260_Q0.5190.58
25_H325_K0.5190.58
310_A315_M0.5180.58
340_V346_D0.5170.57
188_R237_P0.5160.57
217_F253_S0.5150.57
202_Q239_T0.5140.57
129_L216_W0.5140.57
148_R275_E0.5120.57
70_K101_V0.5120.57
156_R165_T0.5110.57
69_L116_K0.5110.57
149_Q158_P0.5110.57
12_I73_V0.5100.56
144_I148_R0.5100.56
213_K254_D0.5080.56
260_Q296_G0.5080.56
104_I154_G0.5080.56
326_L330_I0.5070.56
12_I76_L0.5050.56
160_E166_D0.5040.56
369_A400_Y0.5030.55
371_V400_Y0.5030.55
381_R384_S0.5020.55
330_I335_R0.5000.55
327_P330_I0.5000.55
295_A374_S0.5000.55
136_L174_I0.5000.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qtfA 1 0.7024 100 0.331 Contact Map
3geeA 2 0.8333 100 0.674 Contact Map
3j65o 1 0.7405 100 0.676 Contact Map
1xzpA 4 0.9286 100 0.686 Contact Map
3gehA 2 0.8905 100 0.693 Contact Map
2e87A 1 0.7952 100 0.737 Contact Map
2qm8A 2 0.5857 100 0.76 Contact Map
4a9aA 1 0.6262 100 0.761 Contact Map
3j4jA 1 0.6429 100 0.764 Contact Map
4kjzA 1 0.6452 100 0.77 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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