GREMLIN Database
NFRA2 - FMN reductase [NAD(P)H]
UniProt: P94424 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 249 (238)
Sequences: 1453 (1091)
Seq/√Len: 70.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
52_Q185_E4.2311.00
122_T132_T3.4521.00
80_C155_V3.3551.00
60_I145_L3.3121.00
8_L123_A3.1311.00
23_A26_Q3.0021.00
56_R185_E2.9771.00
81_A110_G2.8891.00
20_E165_H2.8821.00
58_K71_D2.8101.00
150_E186_T2.8001.00
78_L149_L2.5711.00
141_N144_E2.5461.00
14_I170_S2.5091.00
237_R241_E2.4281.00
38_P132_T2.3651.00
30_I121_A2.3601.00
143_Q242_K2.2761.00
3_E6_K2.2671.00
122_T161_L2.2241.00
50_T182_N2.2151.00
138_V145_L2.2041.00
26_Q165_H2.1551.00
50_T75_V2.1521.00
17_Y162_V2.0561.00
18_T167_A2.0151.00
54_K58_K2.0151.00
56_R186_T1.9571.00
130_L165_H1.9421.00
79_F121_A1.9381.00
52_Q184_Q1.9121.00
28_D178_Q1.8571.00
24_Q52_Q1.8421.00
118_L134_P1.8341.00
201_D221_S1.8251.00
146_I243_Q1.8071.00
197_I221_S1.7651.00
144_E147_E1.7521.00
63_L141_N1.7371.00
146_I239_M1.7331.00
59_K62_E1.7331.00
55_E58_K1.7201.00
77_L121_A1.6991.00
29_Q128_L1.6811.00
48_V77_L1.6771.00
37_A116_I1.6771.00
26_Q30_I1.6751.00
34_V121_A1.6741.00
62_E66_G1.6270.99
63_L144_E1.6260.99
133_V162_V1.6200.99
16_S167_A1.6170.99
53_D56_R1.6100.99
31_I182_N1.6050.99
84_N189_N1.5990.99
22_V74_P1.5670.99
108_L111_A1.5620.99
18_T165_H1.5460.99
143_Q147_E1.5370.99
48_V79_F1.5330.99
9_T29_Q1.5020.99
59_K144_E1.4610.99
140_G233_Y1.4350.99
65_G137_A1.4240.98
76_F160_G1.4220.98
26_Q128_L1.4170.98
135_I162_V1.4120.98
53_D185_E1.3930.98
76_F135_I1.3810.98
32_E35_Q1.3420.98
28_D32_E1.3370.98
207_Y211_R1.3350.98
77_L163_I1.3230.98
59_K148_L1.3140.97
24_Q28_D1.3130.97
26_Q130_L1.2670.97
27_L77_L1.2610.97
206_E209_N1.2550.97
201_D204_M1.2390.96
56_R59_K1.2360.96
46_V117_A1.2340.96
22_V25_E1.2270.96
6_K10_D1.2050.96
61_S71_D1.2000.96
105_E139_R1.1940.95
194_T198_Q1.1830.95
38_P122_T1.1730.95
87_K154_Y1.1610.95
132_T163_I1.1580.95
35_Q181_V1.1510.94
27_L31_I1.1270.94
202_E206_E1.1260.94
76_F162_V1.1240.94
153_K243_Q1.1130.93
69_W168_D1.1030.93
118_L159_S1.0980.93
56_R148_L1.0940.93
107_V110_G1.0900.93
5_I124_A1.0890.93
84_N88_I1.0880.93
64_A137_A1.0690.92
125_A132_T1.0690.92
30_I125_A1.0590.92
235_H238_E1.0560.91
205_S219_N1.0480.91
180_A196_H1.0340.91
198_Q201_D1.0320.90
36_S172_K1.0260.90
188_L191_D1.0250.90
54_K186_T1.0220.90
198_Q202_E1.0100.89
122_T134_P1.0090.89
11_H120_T0.9920.89
69_W135_I0.9880.88
55_E59_K0.9870.88
206_E210_K0.9870.88
81_A158_L0.9840.88
32_E178_Q0.9800.88
113_D136_G0.9790.88
195_S198_Q0.9710.88
216_E223_S0.9670.87
238_E242_K0.9660.87
48_V117_A0.9590.87
108_L112_V0.9460.86
107_V240_L0.9440.86
179_E196_H0.9360.86
218_R223_S0.9350.85
117_A121_A0.9330.85
48_V182_N0.9320.85
29_Q33_A0.9240.85
56_R150_E0.9220.85
132_T161_L0.9190.84
112_V159_S0.9150.84
9_T33_A0.9070.84
23_A165_H0.9050.84
24_Q184_Q0.9050.84
61_S64_A0.9030.83
4_V7_S0.9010.83
49_I80_C0.8970.83
47_T183_H0.8870.82
28_D179_E0.8870.82
16_S170_S0.8820.82
40_S105_E0.8820.82
4_V123_A0.8780.82
11_H126_E0.8770.82
83_F110_G0.8760.82
50_T184_Q0.8760.82
6_K9_T0.8750.81
9_T32_E0.8690.81
54_K57_K0.8680.81
31_I50_T0.8590.80
42_N204_M0.8570.80
34_V117_A0.8550.80
115_G159_S0.8510.80
68_P71_D0.8500.80
240_L246_K0.8490.79
237_R246_K0.8470.79
46_V181_V0.8360.78
16_S168_D0.8350.78
4_V127_S0.8330.78
134_P159_S0.8320.78
8_L124_A0.8290.78
235_H239_M0.8250.77
119_G134_P0.8240.77
111_A156_F0.8220.77
171_A212_T0.8210.77
21_P74_P0.8170.77
63_L148_L0.8150.77
68_P72_Q0.8140.77
11_H132_T0.8120.76
8_L33_A0.8110.76
198_Q204_M0.8110.76
5_I29_Q0.8100.76
125_A163_I0.8090.76
87_K153_K0.8090.76
142_P239_M0.8020.75
138_V157_P0.8000.75
201_D219_N0.7980.75
238_E241_E0.7980.75
64_A70_I0.7930.75
14_I168_D0.7900.74
78_L138_V0.7820.74
38_P134_P0.7730.73
73_A162_V0.7610.72
22_V26_Q0.7610.72
84_N155_V0.7590.71
76_F137_A0.7560.71
16_S72_Q0.7540.71
124_A127_S0.7510.71
12_R170_S0.7480.70
26_Q121_A0.7440.70
105_E233_Y0.7410.70
75_V182_N0.7410.70
219_N222_Q0.7400.69
200_Y204_M0.7370.69
5_I128_L0.7370.69
111_A158_L0.7340.69
151_L155_V0.7320.69
151_L187_Y0.7200.67
5_I127_S0.7160.67
74_P130_L0.7160.67
241_E246_K0.7120.66
33_A123_A0.7040.66
52_Q75_V0.7030.65
112_V115_G0.7030.65
110_G156_F0.7020.65
57_K75_V0.6920.64
79_F114_A0.6910.64
79_F118_L0.6890.64
150_E179_E0.6880.64
25_E28_D0.6870.64
194_T202_E0.6850.63
57_K162_V0.6830.63
43_G106_S0.6820.63
191_D195_S0.6750.62
22_V163_I0.6750.62
112_V134_P0.6740.62
46_V115_G0.6720.62
235_H242_K0.6690.62
12_R169_R0.6690.62
22_V28_D0.6660.61
88_I189_N0.6660.61
199_A202_E0.6610.61
40_S140_G0.6610.61
50_T57_K0.6570.60
64_A67_Q0.6560.60
77_L161_L0.6550.60
45_Q109_V0.6540.60
209_N226_S0.6520.60
58_K61_S0.6480.59
121_A161_L0.6480.59
50_T77_L0.6460.59
5_I33_A0.6440.59
218_R222_Q0.6420.59
146_I153_K0.6410.58
17_Y69_W0.6400.58
6_K81_A0.6390.58
215_K218_R0.6370.58
216_E221_S0.6310.57
40_S109_V0.6270.57
135_I220_W0.6270.57
30_I163_I0.6260.57
224_I228_Y0.6250.56
99_E117_A0.6230.56
139_R220_W0.6230.56
11_H38_P0.6220.56
70_I85_R0.6190.56
183_H188_L0.6180.56
108_L236_I0.6150.55
42_N200_Y0.6150.55
153_K244_G0.6130.55
209_N217_T0.6110.55
179_E199_A0.6100.55
51_V78_L0.6070.54
19_D72_Q0.6070.54
32_E58_K0.6070.54
38_P161_L0.6070.54
86_A224_I0.6070.54
31_I37_A0.6020.54
31_I178_Q0.6010.54
201_D211_R0.5990.53
178_Q182_N0.5970.53
10_D169_R0.5960.53
67_Q70_I0.5940.53
57_K73_A0.5930.53
75_V163_I0.5920.52
171_A210_K0.5900.52
48_V121_A0.5900.52
26_Q29_Q0.5890.52
233_Y236_I0.5890.52
5_I9_T0.5870.52
40_S160_G0.5860.52
205_S217_T0.5860.52
208_M211_R0.5850.52
212_T215_K0.5840.52
84_N153_K0.5810.51
60_I138_V0.5810.51
34_V120_T0.5810.51
36_S173_K0.5790.51
144_E148_L0.5760.51
63_L145_L0.5730.50
173_K200_Y0.5720.50
208_M223_S0.5720.50
69_W133_V0.5700.50
96_F226_S0.5680.50
63_L137_A0.5680.50
38_P159_S0.5680.50
38_P126_E0.5680.50
210_K242_K0.5670.49
40_S173_K0.5670.49
47_T136_G0.5660.49
32_E172_K0.5610.49
50_T74_P0.5600.49
10_D172_K0.5600.49
51_V60_I0.5600.49
195_S205_S0.5570.48
156_F239_M0.5560.48
56_R63_L0.5530.48
116_I120_T0.5520.48
156_F245_F0.5500.47
33_A124_A0.5490.47
43_G228_Y0.5480.47
39_S233_Y0.5480.47
142_P236_I0.5470.47
61_S66_G0.5460.47
81_A155_V0.5450.47
46_V114_A0.5450.47
142_P157_P0.5440.47
21_P75_V0.5430.47
61_S137_A0.5430.47
51_V149_L0.5430.47
40_S139_R0.5410.46
224_I230_R0.5400.46
40_S136_G0.5400.46
28_D198_Q0.5390.46
7_S10_D0.5390.46
234_P238_E0.5380.46
224_I232_Y0.5380.46
21_P52_Q0.5350.46
208_M216_E0.5350.46
58_K62_E0.5320.45
38_P173_K0.5300.45
105_E112_V0.5300.45
131_G166_P0.5280.45
6_K32_E0.5270.45
69_W134_P0.5250.44
120_T123_A0.5240.44
11_H123_A0.5240.44
22_V128_L0.5240.44
195_S199_A0.5240.44
74_P165_H0.5230.44
25_E153_K0.5230.44
105_E109_V0.5210.44
77_L182_N0.5210.44
30_I128_L0.5200.44
41_I134_P0.5190.44
27_L182_N0.5190.44
150_E189_N0.5180.44
203_Q226_S0.5180.44
44_Q176_L0.5160.43
86_A142_P0.5160.43
224_I229_E0.5160.43
8_L120_T0.5160.43
46_V75_V0.5150.43
112_V139_R0.5130.43
24_Q27_L0.5120.43
116_I119_G0.5120.43
60_I64_A0.5080.42
72_Q202_E0.5080.42
73_A76_F0.5070.42
87_K98_M0.5070.42
44_Q200_Y0.5060.42
43_G86_A0.5040.42
117_A181_V0.5030.42
84_N154_Y0.5020.42
83_F111_A0.5010.41
115_G220_W0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zchA 2 0.988 100 0.24 Contact Map
3n2sA 2 0.988 100 0.248 Contact Map
1f5vA 2 0.9639 100 0.255 Contact Map
1bkjA 2 0.9237 100 0.256 Contact Map
3eofA 2 0.9398 100 0.338 Contact Map
3gagA 4 0.739 100 0.495 Contact Map
3bemA 2 0.7309 100 0.496 Contact Map
1noxA 2 0.7349 100 0.498 Contact Map
3gbhA 3 0.7309 100 0.5 Contact Map
3ge6A 2 0.7349 100 0.501 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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